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Radiosensitivity and the Influence of Gamma Rays Irradiation on Local Samosir Shallots Mariati Sinuraya; Rosmayati Rosmayati; Hasanuddin Hasanuddin; Diana Sofia Hanafiah
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 2 (2015): Life Sciences
Publisher : Syiah Kuala University

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Bulbs of localSamosir Shallot with the weight ranging from 1,3 to1,7 g were irradiated by several doses of gamma rays in order to investigate the radiosensitivity and the effects of irradiatons on the plants. Dry bulbs 2,5 months after harvest were exposed to gamma raysradiation ranging from 0 Gy to 20 Gy to determine their responses to radiation stress and the effective radiation dose for identification of Lethal dose 50 (LD50). Percentage of shoot growth was measured on 35th days after planting. The variation in morphological and agronomic characters were also determined. The results indicated that increasing doses of gamma irradiation had significant effect on shoot growth. Increasing in gamma rays doses from 0 Gy to 11Gy had little effect on percentage of shoot growth.With the increase in radiation dosesabove 11Gy, agreat reduction in percentage of shoot growth was observed in irradiated bulbs as compared to control. The LD50 values of local Samosir Shallot determined from linear regression analysis (using Curve-fit Analysis software) based on percentage of regenerated shoot growth was 11.60 Gy. There were also siqnificantdifferenciesbetweenregenerated plants growth from irradiated bulbs and control (unirradiated).Treated bulbs produced shorther plant lenght and less leave number

Simulation of sea surface temperature (SST) and sea surface salinity (SSS) in the Bay of Bengal Syamsul Rizal; . Muhammad; Taufiq Iskandar; Ichsan Setiawan; Agus Satriadi,; . Radinal
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 2 (2011): Engineering
Publisher : Syiah Kuala University

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The simulation of Bay of Bengal (included Andaman Sea) has been done. This investigation used equation of motion (Navier-Stokes equation). The equation of motion was solved by means of Hamburg Shelf Ocean Model (HAMSOM). The analysis is done for the year of 2007. The National Centers for Environmental Prediction (NCEP) data for year of 2007 is used to force the Bay of Bengal. The sea surface temperature (SST) and sea surface salinity (SSS) have been obtained and analyzed. The highest SST occurs in April 2007, while the lowest SST occurs in October 2007. The pattern of SST depends on the wind vector. From January untill June 2007, the SSS pattern is a west-east pattern. The SSS value is lower in the east and higher in the west. From July untill December, the higher value of SSS is generally in the middle of the Bay of Bengal. Generally, the value of SSS is higher in July and August, while in December and January the value of SSS is lower. Some results have been compared and consistent with the study of Vinayachandran dan Kurian (2008) and Vinayachandran and Yamagata (1998).

Asses humoral immunity of wistar strains rats (Rattus norvegicus L.) which given ethanol extractbuas buas (Premna pubescens Blume) leaves Martina Restuati; Restianna Eliawaty Simanjuntak
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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The aim of the research was to to assess the effect of ethanol extract Premna pubescens leaves on humoral immunity and the body weight of rats (Rattus norvegicus L.). The white rats use 24 tails and devided into 4 groups with each group replicates 6 times. There are A0 as control which give with aquadest, A1 give with ethanol extract of Premna pubescens leaves, A2 give with ethanol extract of Premna pubescens leaves and Sheep Red Blood Cells (SRBC), and A3 give with SRBC only. White Wistar rats aged 3 months with body weight 100-300 grams. Aquadest and ethanol extracts of Premna pubescens given orally every day and SRBC given by injections on the 8th and 15th day . The rats blood taken by decapitation on 31th day. Measurements antibody titer of the rat as humoral immunity used hemagglutination method using a V microplate 96. The results were analyzed statistically with ANOVA. The result showed that the antibody titer at A2 increased significantly , where t (65,78) t table (4,94). This suggests that the ethanol extract of Premna pubescens leaves influence the humoral imunnity of Wistar strains rats using SRBC, The result of body weight statistically, tcount 0.23 ttable 3.10. It means that ethanol extract of Premna pubescens leaves did not influence the body weigh.

Optimization of Glucose as Source of Bioethanol from Reed (Imperatacylindrica) Using Cellulase Enzyme at Various pH and Substrate Particle Size Eti Indarti; Yusya Abubakar; Normalina Arpi; . Satriana; Yuza Arfiansyah
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 2 (2012): Engineering
Publisher : Syiah Kuala University

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Due to increasing demand of petroleum and limited source of fossil fuel, it isneeded to explore and find source of a renewable energy. The biomass from agricultural residues, forest and farm waste has been known as a great source of energy and can be converted into biogas, bioethanol, biodiesel, etc. Reed which is abundant in Indonesia is one of potential sources of lignocellulose biomass that can be utilized as (Imperata cylindrica) a source of renewable energy such as bioethanol. The aim of this study was to optimize yield of glucose from reed using cellulose enzyme. Factors used in this research werepH and substrate particle size. Reed particle sizes were 80 and 100 mesh and pH of the solution were 4.2, 4.8 and 5.5. The results showedthat dried reedwith100 meshes in particle size which hydrolysed in enzyme solution with pH 5.5 at 37oC for 72 hours, producedthe highest yield of glucose in the amount of 80.2 mg/ml (8.02 %). When the incubation time was extended, glucose yield increased to 121.8 mg/ml (12.1%) at 96 hours and then decreased as the incubation time was extended further

Effect of NPK fertilizer and biochar residue on paddy growth and yield of second planting Diana Samira; . Sufardi; . Zaitun; . Chairunas; Anischan Gani; Peter Slavich; Malem McLeod
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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The objective of experiment was to study effects of NPK fertilizer and Biochar residue on paddy growth and yield of second planting. The research was conducted at Empetrieng village, Aceh Besar district, Aceh Province. The experimental arranged in a randomized complate block design with two factors and four replications. Biochar consisted of two levels, i.e. without biochar residue and with biochar residue 10 ton ha-1. NPK fertilizer consisted of three levels, i.e. without NPK, NPK 60 kg ha-1, and NPK 120 kg ha-1. The result showed that application of NPK significantly affected, plant height 35, 45 and 90 day after planting (DAP), number of tiller 35 and 45 DAP, number of panicle per clump, number of total grain per panicle, percentage of unfilled grain, percentage of filled grain per panicle, 1.000 grain weight, and potential yield per ha. Biochar Residue significantly affected potential yield per ha.

Biodegradable Plastic from Cassava Waste using Sorbitol as Plasticizer Wahyu Rinaldi; Mirna Rahman Lubis; Umi Fathanah
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 5, No 1 (2015): Engineering
Publisher : Syiah Kuala University

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Cassava waste can be utilized as raw material for biodegradable plastics. This research studied the influence of sorbitol, carboxymethyl cellulose (CMC) and the gelatinization temperature in the preparation of biodegradable plastic from cassava waste. 12 gram of starch from cassava waste was stired with 68.2 gram of water-ethanol solution at 75 rpm and is fixed during this study. While the amount of sorbitol, CMC and gelatinization temperature are varied. The best results are showed at 44.90% in water absorption, 101.4 MPa in tensile strength, 76.57% in elongation, 4.17 MPa in Young's modulus, and decomposition time are 64, 67, and 79 days.

Ovitrap use in epidemiology study of Aedes aegypti and Aedes albopictus in Kuta Alam sub-district Banda Aceh, Indonesia Athaillah Farida; Abu Hasan Ahmad; Upik K. Hadi; Muhammad Hambal; Yudha Fahrimal; Rimaya Shafitri
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 1, No 1 (2011): Life Sciences
Publisher : Syiah Kuala University

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A study on the mosquitoes Aedes spp. has been conducted in Kuta Alam Sub-district, Banda Aceh, to find out the percentage and distribution of positive ovitrap with eggs of Aedes aegypti and A. albopictus, based on location of ovitrap, level of rainfall as well as different landuse of settlement areas. Sampling was conducted by setting up ovitrap monthly, from January to December 2010 in settlement area that have more trees versus less trees, both indoor and outdoor. Data of the ovitrap percentage that was proved to be positive with eggs of A. aegypti and A. albopictus was analized using Student T-Test. The study revealed that rainfall value did not significantly affect (P0.05) the percentage of positive A. aegypti and A. albopictus ovitraps and neither did the location of ovitrap (indoor versus outdoor). The settlement areas with more trees have significantly higher percentage of ovitraps containing A. albopictus than areas with less trees, but not significant for A. aegypti (P0.05). It was suggested that rainfall and location of ovitrap did not influence the number of ovitrap containing Aedes aegypti and Aedes albopictus eggs, while settlement landuse influenced significantly the percentage of positive ovitraps for Aedes albopictus, but not for Aedes. aegypti. Between two villages in Kuta Alam sub-district, it was found out that Gampong Beurawe was dominated by Aedes albopictus while Gampong Kuta Alam by Aedes aegypti. Overall, the sensitivity of ovitrap in Kuta Alam subdistrict was 83,7%. The usage of ovitrap is thus recommended for monitoring of Aedes spp. control programs

Optimum allocation unit catching small pelagic fish in the water in Kei Island Southeast Maluku J. Tahapary; Bruri B. Tumiwa
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 3, No 1 (2013): Life Sciences
Publisher : Syiah Kuala University

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Small pelagic fish is one of the fish are potential resources and high compared to other kinds of fish in Kei Island Southeast Maluku. The aim of this research is to determine the optimum allocation unit arrest in the utilization of small pelagic fish in the Kei Island waters. An instrument grap used for catching small pelagic fish in the waters Kei Island is a lift net, purse seine, drift gill net and encircling gill net. A method of collecting data covering data resources fish, fuel, perational cost labor, the number of units arrest, and tracing of references pertaining to an object research. Analysis of data using linear goal programming ( LGP ) to provide information is important in allocating a unit of catching small pelagic fishoptimally, and that is how allocation grap, optimal an instrument used how big achievement the purpose of which is willed according to the target, and just how much resources be used in achieving its goal. A kind of small pelagic fish widely distributed in Kei Island waters is Decapterus sp., Rastrelliger sp., Selaroides spp., Sardinela sp., Clupea s.p and Stolephorus sp. The result of this research is optimum allocation unit catching small pelagic fish in the Kei Island waters for the implementation of allocation technology usually in the utilization of resources fish

Crystallization of thermostable lipase from Geobacillus thermodenitrificans IBRL-nra Anuradha Balan; Darah Ibrahim; Rashidah Abdul Rahim
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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Crystallization of a protein is a process of formation of a solid crystal from a homogeneous solution which leads to the knowledge of its three dimensional structures and it plays important role in designing and engineering protein for specific purposes. Thermostable lipases are commercially significant for their potential use in industries as it is stable and active in organic solvents, possess a wide range of substrate specificity, resistance to high temperature and chemical denaturation. The purified thermostable lipase from Geobacillus thermodenitrificans IBRL-nra was screened for crystal formation using Hampton Research screening kit, HR2-121 and HR2-122 using hanging drop vapour diffusion and microbatch methods. Microcrystals and rod clusters grew from mixtures in formulation 14, 20, 21 and 38 of HR2-121 and formulation 14, 16, 32 and 45 of HR2-122. Further enhancement of the parameters was carried out for the formation of well-defined crystal. The parameters tested are pH (4.6, 6.5, 7.5 and 8.5) and concentration of CaCl2 (0.15-0.38M)

Utilisation of DNA barcoding for identification of fish products G. Ahmad Faisal; M. N. Siti Azizah; M. N. Darlina
Proceedings of The Annual International Conference, Syiah Kuala University - Life Sciences & Engineering Chapter Vol 2, No 1 (2012): Life Sciences
Publisher : Syiah Kuala University

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DNA barcoding has been accepted nowadays as a globally accessible tool to delimitate and identify new species, as it can provide accurate and automated species identification through the use of standard gene, Cytochrome oxidase I (COI). As a result, DNA barcoding has become a perfect molecular tool to identify fish products and detect wrongly labelled fish in the market as it can identify fish species even without the presenceof complete morphological characteristics. Here we collected samples from sushi restaurants to investigate and identify the precise species identity of raw fish sushi products because most of them were labelled with only common names such as Tuna, Salmon and Butterfish without providing the scientific name of the fishes. Species identities of seven specimens from two sushi restaurants were DNA barcoded utilising the cytochrome oxidase I (COI) gene. The data were BLAST with GenBank and BOLD to confirm species identification. The results showed 100% matches that there was nosubstitution and wrongly labelling of species as the Salmon sushi matched the database sequences of Salmo salar, Tuna to Thunnus albacares and Butterfish to Lepidocybium flavobrunneum. The investigation of these fishproduct true identities should be carried out routinely to ensure consumer protection from mislabelling and substitution for a cheaper fishin order to avoid health issuessuch as allergic reaction due to intake of the wronglylabelled product

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Contact Name
-

Contact Email
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Phone
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Journal Mail Official
-

Editorial Address
-

Location
Kab. aceh besar,

Aceh

INDONESIA