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Lussana Rossita Dewi
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Bioma : Jurnal Ilmiah Biologi
Published by Universitas PGRI Semarang
ISSN : 20865481     EISSN : 25499890     DOI : https://doi.org/10.26877
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Jurnal Ilmiah Biologi focuses on the publication of result’s research related with the major of biology and biology education. For the major of biology, including systematics, physiology and metabolism, ecology, bioconservation, cell and developmental biology, microbiology, genetics, molecular biology, biotechnology, and bioinformatics. For the major of biology education, including biology curriculum, teaching biology, instructional media, and assessment.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 70 Documents
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THE IMPACT OF INADEQUATE LABORATORY PERSONNEL (LABORATORY HEADS, TECHNICIANS, AND ASSISTANTS) ON BIOLOGY PRACTICUM IMPLEMENTATION Ana, Ana; Wulandari, Dhea Aprilia Ayu; Mafimudah, Rifatul; Sari, Fatma Mutia; Saifuddin, Much Fuad; Puspitasari, Etika Dyah
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/3s4exd29

Abstract

This study aims to examine the limitations of human resources (HR) in school laboratories and their impact on the implementation of biology practicum activities at the senior high school level. A descriptive qualitative method was employed in Kotagede District, Yogyakarta, using purposive sampling to select two schools with active biology laboratories. Data were collected through in-depth interviews with laboratory personnel and biology teachers to explore the availability, involvement, and roles of laboratory staff in supporting practicum activities. The findings revealed significant differences between the two schools. School A had a complete laboratory staff, including a head of the laboratory and a laboratory assistant, which allowed practicum activities to be conducted in a structured and systematic manner, effectively supporting students' conceptual understanding and scientific understanding. supporting students' conceptual understanding and scientific skills. In contrast, School B lacked laboratory personnel, resulting in all practicum responsibilities being assigned to the biology teacher, which led to suboptimal implementation and limited student learning experiences. Therefore, efforts to increase the availability and competence of laboratory personnel in accordance with national standards are necessary. The presence of professional laboratory staff plays a crucial role in creating effective, meaningful, and skill-oriented biology learning through practicum-based approaches.    
ANATOMICAL STRUCTURE STUDY OF Solanum torvum Sw. Agustina, Dwi; Ermayanti
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v14i2.2685

Abstract

The Anatomy of Tekokak (Solanum torvum Sw.) is a plant that grows wild in the forest and its fruit is used as a vegetable. This study aims to complete the study of the anatomical structure of tekokak and identify typical microscopic characteristics that can be used as specific markers. This research is important in order to contribute to the scientific documentation of local plant taxonomy and support the utilization of plants in the fields of pharmacy, conservation, and as an a reference for botanical education in Biology. The research data were analyzed using a qualitative descriptive method by comparing the characteristics of the samples found with references. The results of this study include image data and qualitative descriptive data obtained from the anatomy of the roots, stems and leaves of Solanum torvum Sw The root of S. torvum Sw. consists of the epidermis, exodermis, a cortex layer made up of cortex parenchyma, endodermis with Casparian strips, a pericycle or pericambium layer, special medullary ray structures, and vascular tissues, xylem, and phloem. The stem part is composed of an epidermis with star-shaped non-glandular trichomes, lamellar collenchyma, cortex, endodermis layer, pericycle, vascular tissue comprising xylem and phloem, and pith. The anatomical difference between unbranched and branched stems lies in the arrangement of the vascular bundles, the thickness of the supportive tissue, and the complexity of the vascular tissue, indicating structural adaptations to the position and function of the stem. On the stem found scalariform trachea, annular trachea, reticulate trachea, thickened reticulate trachea, tracheid, fiber tracheid and libriform fiber. The leaf cross-section shows adaxial epidermis with cuboid-shaped cells and anisocytic stomata, palisade parenchyma, spongy parenchyma, abaxial epidermis with anomocytic stomata, and non-glandular star-shaped trichomes.
VISUAL REPRESENTATIONS ANALYSIS OF SENIOR HIGH SCHOOL BIOLOGY TEXTBOOKS ABOUT THE HUMAN MOVEMENT SYSTEM Antasena, Muhamad Yafi B.; Agung Wijaya Subiantoro
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v14i2.2711

Abstract

This study aims to identify the proportions and types of visual representations in Grade XI Biology textbooks, specifically on the topic of the Human Locomotion System. Visual content in Biology textbooks plays a crucial role in facilitating students’ understanding of biological concepts; however, previous studies have shown that such content remains limited in depth and rarely connects biological material to real-life contexts. The objective of this research was to examine the functions and forms of visual representations in selected textbooks, and to evaluate their alignment with principles of effective visual representation. Employing a qualitative content analysis approach, four textbooks were analyzed using a classification framework that classified the visual representations by their function (decorative, illustrative, explanatory, complementary) and by their form (e.g. illustrations, diagrams, tables, maps). The results revealed that visual representations were predominantly illustrative in both function and form across all Biology textbook samples. Among the samples, the YT textbook demonstrated the highest quantity and diversity of visual content, while KM had the fewest. These findings suggest that the textbooks have not fully adhered to the principles of effective visual representation, which may limiting students’ understanding of complex biological concepts. It is recommended that future editions increase both the quantity and variation of visual content to enhance pedagogical effectiveness and promote deeper learning.
PHYTOCHEMICAL SCREENING AND ANTIBACTERIAL ACTIVITY OF SOFT CORAL Nephthea sp. EXTRACT ISOLATED AGAINST Escherichia coli Setyaningrum, Tri Wahyu; Nurulfadilah; Candri, Dining Aidil; Dharmayani, Ni Komang Tri; Ghazali, Mursal; Faturrahman
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 1 (2026): April 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i1.3070

Abstract

This study aimed to evaluate the antibacterial potential of Nephthea sp. extract against Escherichia coli and to analyze the phytochemical constituents present in Nephthea sp. using various solvents. The extraction process was carried out using the maceration method with three different solvents: n-hexane, ethyl acetate, and methanol. The resulting extracts were analyzed through phytochemical screening and antibacterial activity assays. The results showed that Nephthea sp. extract contained several classes of bioactive metabolites, including alkaloids, flavonoids, triterpenoids, steroids, tannins, and saponins. Antibacterial testing demonstrated that the ethyl acetate extract produced the largest inhibition zone, measuring 3.16 mm. Based on the ANOVA test results, the 25% concentration showed a significant effect on the inhibition zone. Based on phytochemical analysis, the ethyl acetate extract contained triterpenoid compounds, whereas the other solvents did not
MORPHOLOGICAL CHARACTERIZATION OF BRAIN TUMOR TISSUE IN MRI IMAGES USING CNN AND TRANSFER LEARNING Dafa Fadhilah Hilmi; Aji Prasetya Wibawa; Ardha Ardhana Putra Agustavada; Abdullah Sholum; Felix Andika Dwiyanto
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 1 (2026): April 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i1.3550

Abstract

This study evaluates the role of computational pattern recognition as an observational method for analyzing morphological characteristics of brain tumor tissue in MRI data. A total of 6,056 labeled MRI images, including glioma, meningioma, and pituitary tumor cases, were examined. The images were standardized to maintain uniform structural representation and processed using three convolutional-based architectures: a baseline CNN, MobileNetV2, and EfficientNet-B0. Model performance was assessed using accuracy, precision, recall, F1-score, AUC-ROC, and a confusion matrix. The findings show variation in identification performance across tumor categories, with pituitary tumors consistently recognized, while misclassification predominantly occurred between glioma and meningioma. Models based on transfer learning achieved stronger agreement with the reference labels than the baseline CNN, with MobileNetV2 demonstrating the most stable performance. The recurrence of similar misclassification patterns across models suggests the presence of shared morphological characteristics in MRI representations of certain tumor types. Overall, the results support the use of computational image analysis as a structured observational framework that enables consistent evaluation of brain tumor tissue morphology in MRI, providing complementary insights for biological interpretation.
SCREENING OF LDPE PLASTIC-WASTE-DEGRADING BACTERIA USING WINOGRADSKY COLUMN Bowo Sugiharto; Alifia Harisma Rustanti
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 1 (2026): April 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i1.3201

Abstract

This study aims to screen Low-Density Polyethylene (LDPE)-degrading bacteria from three highly plastic-contaminated environments: the Jatimalang River stream, Bekonang Market soil, and Joho Village Waste Disposal Site. The research used the Winogradsky column method with two incubation periods: 1 month and 2 months. This research was conducted with 3 repetitions using a descriptive-quantitative data analysis approach, and was a preliminary screening. Observed parameters included the percentage of plastic degradation, biofilm thickness, physical changes in LDPE, and bacterial morphology isolated using Nutrient Agar. The results showed that the highest degradation occurred in the waste-disposal soil (TPS), with values of 34.37% (1 month) and 40.62% (2 months), followed by river soil (22.72% and 27.27%) and market soil (13.63% and 18.18%). Biofilm thickness was also most significant in TPS samples, reaching 0.57 ± 0.01 and 0.91 ± 0.03 μm. Physical changes included yellowish to brownish discoloration, increased roughness, and enhanced elasticity of LDPE. All bacterial isolates exhibited irregular colony forms, lobate margins, flat elevations, and cream-to-yellow pigmentation. Gram staining identified all isolates as Gram-positive bacteria. These findings indicate that plastic-contaminated environments hold strong potential as natural sources of LDPE-degrading bacteria.
EFFECTS OF PRETREATMENT METHODS ON THE LIGNOCELLULOSIC COMPOSITION OF SALACCA MIDRIBS Berlian Simanjuntak; Isabella Kristin Hotma Silalahi
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 1 (2026): April 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i1.3473

Abstract

This study aims to determine the effect of pretreatment on hemicellulose, cellulose, and lignin content of Salacca midrib. Unlike previous studies, this study was designed to evaluate the effects of single-, double-, and triple-stage pretreatments on the sallaca midrib. The study was also designed to evaluate the differences in the physical and biological pretreatment methods. The pretreatments applied consist of physical, chemical, biological, physicochemical, chemical-biological, physical-biological, and physicochemical-biological pretreatments. Physical pretreatment is carried out in an autoclave at 121 °C, chemical pretreatment using Sodium Hydroxide (NaOH) 4%, and biological pretreatment using Debaryomyces hansenii. Based on the ANOVA test, the various pretreatment methods have a significant effect on lignocellulose content. The highest hemicellulose content was the chemical pretreatment at 34.13%, the cellulose content was the chemical pretreatment at 40.85%, and the highest lignin reduction was the physical-chemical-biological at 27.15%
PROFILING GRADE STUDENTS’ SCIENCE PROCESS SKILLS IN LEARNING INHERITANCE OF TRAITS Nikmah Alfiatun; Endah Rita Sulistya Dewi; M. Syaipul Hayat
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 1 (2026): April 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i1.3637

Abstract

This study aims to investigate and comprehensively describe the profile of Science Process Skills (SPS) among grade XII students in the biology material on Inheritance of Traits at SMA Negeri 1 Ngawen. The study uses a quantitative approach, employing the Research and Development (R&D) method and adapting the ADDIE development model. Quantitative data collection was carried out through tests using SPS assessment instruments in the form of structured essay questions that include 6 main indicators: observing, classifying, predicting, measuring and using numbers, concluding, and communicating. The results of data analysis show a very uneven distribution of students' SPS abilities: only 1.47% of students are included in the "good" category, 19.12% are categorized as "sufficient", 20.59% are categorized as "less", and the absolute majority of 58.82% are in the "very less" category. Cumulatively, the average student KPS score was 41.67% (very poor). This finding underscores the need for a radical reconstruction of biology learning strategies by shifting toward a scientific approach integrated with the STEAM framework to boost students' scientific investigation skills.    
STUDENTS' HABITS OF MIND IN BIOLOGY LEARNING AS A REPRESENTATION DEEP LEARNING PREPARATION Dina Alfiyana; Muhammad Syaipul Hayat; Praptining Rahayu
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 1 (2026): April 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i1.3691

Abstract

This study aims to determine students' readiness and thinking habits in deep learning-based biology instruction, based on this premise. This research was conducted using descriptive qualitative and quantitative methods, in which data were collected through questionnaires and observations during the learning process, with interviews to support students' opinions. This study involved 103 students from grade X digital media at SMA Islam Sultan Agung 1 Semarang, who were studying biology material on environmental change. The research collected through the questionnaire showed that the self-regulation score was 82 (good), the critical thinking score was 80 (good), while the creative thinking score was 68.50 (enough). The observation results showed that the self-regulation score was 80.56 (good), the critical thinking score was 62.50 (enough), and the creative thinking score was 69.44 (enough). Interview data corroborate that self-regulation is optimal, but critical and creative thinking have not become a habit in students' thinking. Therefore, the thinking habits of class X students at SMA Islam Sultan Agung 1 Semarang still need improvement, especially in creativity and critical thinking.
APPLICATION OF THE PROJECT-BASED LEARNING MODEL ON PROKARYOTIC MATERIAL TO IMPROVE COGNITIVE, AFFECTIVE, AND PSYCHOMOTOR LEARNING OUTCOMES OF GRADE ilmi maehanifa; Atip Nurwahyunani; Praptining Rahayu
BIOMA : Jurnal Ilmiah Biologi Vol. 15 No. 1 (2026): April 2026
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v15i1.3686

Abstract

This study aimed to determine the effect of the Project-Based Learning (PjBL) model on students' cognitive, affective, and psychomotor learning outcomes in learning prokaryotic material. The research was conducted at SMA Kesatrian 2 Semarang using a quasi-experimental method with a nonequivalent control group design. The final sample consisted of 25 students in the experimental class and 29 students in the control class, selected through purposive sampling. The research instruments included cognitive learning outcome tests, affective observation sheets, and psychomotor observation sheets. Data were analyzed using the Shapiro–Wilk normality test, Levene's homogeneity test, the Mann–Whitney U test, the independent samples t-test, and the N-Gain test. The results showed a significant difference in cognitive learning outcomes (p < 0.001) and affective learning outcomes (p = 0.044) between the experimental and control groups. However, no significant difference was found in psychomotor learning outcomes (p = 0.282). The average N-Gain scores were 0.63 for the experimental group and 0.69 for the control group, both categorized as moderate. Because the two groups differed significantly in their initial abilities before the intervention, the findings should be interpreted with caution.

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