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Bioma : Jurnal Ilmiah Biologi
Published by Universitas PGRI Semarang
ISSN : 20865481     EISSN : 25499890     DOI : https://doi.org/10.26877
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Jurnal Ilmiah Biologi focuses on the publication of result’s research related with the major of biology and biology education. For the major of biology, including systematics, physiology and metabolism, ecology, bioconservation, cell and developmental biology, microbiology, genetics, molecular biology, biotechnology, and bioinformatics. For the major of biology education, including biology curriculum, teaching biology, instructional media, and assessment.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 63 Documents
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ANALYSIS OF LEARNING PREFERENCES AND STYLES OF STUDENTS IN AGRIBUSINESS PLANT EDUCATION Suratman, Ikhsan Hidayat; Nurwahyunani, Atip; Cahyati, Zubaidah Gesit
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/8sww6752

Abstract

Agribusiness vocational education has a strategic role in preparing skilled workers who are able to compete in the world of work. This study aims to analyze the preferences and learning styles of students in the Food Crops and Horticulture Agribusiness program at SMK Negeri 1 Bawen. The research method used is quantitative descriptive with data collection through questionnaires and interviews. The main focus of this study includes preferences for learning methods, learning methods, enthusiasm levels, and other supporting factors such as access to agricultural land and learning facilities. The results showed that 48.28% of students preferred a combination of theory and practice learning methods because they provided a deeper understanding and were relevant to the needs of the world of work. As many as 79.31% of students chose to study in groups because they allowed discussion and collaboration, while 20.69% of students preferred independent learning to focus on individual understanding. The level of student enthusiasm for agribusiness learning varied, with the majority showing high involvement especially in field practice activities. Supporting factors such as access to agricultural land, practice facilities, and teacher support played a significant role in increasing student motivation and skills. This study concludes that a balanced learning approach between theory and practice, supported by adequate facilities and cooperation with the industrial world, can optimize student learning outcomes. Recommendations include the development of an industry-based curriculum and increased support for students who do not have access to agribusiness land.
ISOLATION OF AnsB GENE FRAGMENT ENCODING L-ASPARAGINASE 2 ENZYME FROM Serratia plymuthica UBCF_13 AND IT’S IN-SILICO DOMAIN CHARACTERISTIC Ananda, Abi Awfa Rahman; Nursyafi, Fauzan Syarif; Aliya, Lisana Shidiqqin; Hasibuan, Imron Martua; Alioes, Yustini; Endrinaldi , Endrinaldi; Adrial, Adrial; Elmatris , Elmatris; Jamsari, Jamsari; Lily Syukriani
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/n5mfhy69

Abstract

Acute lymphoblastic leukemia(ALL) is one of the cancer diseases often occurs in children and causes high mortality in children. One of the chemotherapy treatment suggested is using L-Asparaginase 2. However due to its difficult production process making this approach expensive for the public. Therefore production technology of this enzyme is crucial enabling cheaper for ALL treatment. This study aimed to isolate the AnsB gene sequence from Serratia plymuthica UBCF_13 and perform its further in-silico analysis. The research was started by designing specific primers for the AnsB gene, isolating the AnsB gene fragment using PCR-based approach, sequencing the AnsB gene fragment, cloning the fragment to the plasmid vector and further transformed into E. coli DH5α cell. Further data analysis was carried out using some bioinformatics tools such as BLAST, MEGA X, I-TASSER InterPro. Sequence data result successfully verified that the full length of AnsB gene is 1047 bp. InterPro analysis indicated that the L-Asparaginase 2 from S. plymuthica UBCF_13 has 2 domains, namely L-Asparaginase N-terminal spanning from amino acid 26 to 216, while its C-terminal spanned from amino acid 235 to 345. The physical fragment of the gene was also successfully cloned to the pGEM-T Easy vector and subsequently transformed into E. coli DH5α cell. This result provided information for alternative sources of L-Asparaginase 2 and it’s possible engineering.
CLASS EVALUATION: DEVELOPMENT OF HIGHER ORDER THINKING SKILLS TEST INSTRUMENTS IN DIGESTIVE SYSTEM LEARNING Sumiati, Iis; Windayani, Neneng; Farida, Ida; Cahyanto, Tri
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/x6n7mj33

Abstract

In an era of ever-developing education, it is important to ensure that the evaluation tools used in the classroom can reflect students’ true abilities in understanding complex concepts. Measuring higher order thinking skills (HOTs) is an integral part of assessing student understanding. The aim of the research is to develop and validate a test instrument specifically designed to measure higher order thinking skills in digestive system learning at the secondary school level. The Higher Order Thinking Skills framework by Resnick, 1987 was used to develop the instrument. This research uses a Research and Development Design (R&D) approach by adapting the ADDIE model which consists of five stages, that is: Analysis, Design, Development, Implementation and Evaluation. The research sample consisted of 30 class IX students at Al-Qona'ah Middle School who were selected using a simple random sampling technique. The research results showed that there were eight out of ten valid questions with high reliability that were ready to be used to comprehensively measure students' HOTs abilities.
PHYTOREMEDIATION OF TSS AND TDS FROM LANDFILL LEACHATE BY Equisetum hyemale Ulfah, Maria; Kurniawati, Septiana; Nurwahyunani, Atip; Wijaya, Angelica Tricia
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 1 (2025): April 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/ayvxv673

Abstract

Leachate contains TSS (Total Suspended Solid) and TDS (Total Dissolved Solid) which causes turbidity and a blackish brown color in the leachate. One effort that can be made to deal with environmental pollution caused by leachate is phytoremediation. This research aims to determine the effectiveness of water bamboo as a phytoremediation agent for TSS and TDS from landfill leachate. This research is an experimental study with a completely randomized design (CRD) with three treatment levels and three repetitions. The treatments given in this research were three variations of water bamboo, namely P0 : without water bamboo, P1: 750 g water bamboo, and P2: 1.000 g water bamboo. Based on research results, water bamboo is not effective in reducing TSS and TDS. The most optimal percentage reduction in TSS occurred in the P2 on the third day of the study (16.83%). The most optimal percentage reduction in TDS occurred in the P1 on the seventh day of the study (6.56%). The results of research show that phytoremediation can reduce Jatibarang Landfill leachate, especially TSS and TDS levels accumulated by the roots of water bamboo.
IMPLEMENTATION OF TPACK APPROACH TOWARD CRITICAL THINKING ABILITY AND COGNITIVE LEARNING OUTCOMES OF STUDENTS OF SMA NEGERI 1 JAKENAN ON ENVIRONMENTAL CHANGE MATERIAL Khoiril, Dina; Nurwahyunani, Atip
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.356

Abstract

This study aims to determine the effect of implementing the TPACK approach to developing critical thinking skills and student learning outcomes. The design used in this research is Experimental (Quasi-experimental) with Non Equivalent Control Group Design. The research subjects consisted of 72 high school students who were divided into two homogeneous classes, namely 36 students in the control class and 36 students in the experimental class. Students in the control class were taught conventional learning and the experimental class was taught with the TPACK approach. The TPACK approach to critical thinking skills is sufficient. Learning with the TPACK approach is more effective in improving learning outcomes than conventional learning. The results of the tcount test in the experimental class were 63.375 and the ttable was 1.666 while the control class the tcount test results were 25.102 and the ttable was 1.666.
COLIFORM CONTAMINATION LEVEL OF WASHING WATER AT STREET VENDORS TENT STALLS IN YOGYAKARTA Amarantini, Charis; Budiarso, Tri Yahya; -, Tri Wahyuni Sekar Sari
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.365

Abstract

Food quality in Indonesia is significantly influenced by street vendors, particularly from a microbiological aspect. The use of unclean washing water in these settings can cause tableware contamination, increasing the risk of food-borne diseases. Therefore, this research aimed to determine the level of coliform contamination in washing water of street vendors. A total of 21 samples were collected from different locations, isolated, and identified morphologically and biochemically. These samples were resuscitated in 1% peptone medium for 12 hours and inoculated on Chromocult Coliform Agar (CCA) medium. Purified coliform colonies were tested morphologically and biochemically to the genus level and confirmed using API 20E KIT reagents. The results showed coliform contamination levels ranging from 5.1 x 105 to 2.7 x 108 CFU/mL, exceeding the quality standard. Confirmation results from testing using API 20E with ID ≥ 95% found bacteria contaminants, including Escherichia coli, Klebsiella pneumoniae subsp. Pneumoniae and Pantoea spp. These results showed the urgent need to recognize the risks associated with tableware contamination at tent stalls. Keywords: API 20E, Coliform, Street Vendors, Washing Water.
DIGITALIZATION TRANSFORMATION OF ESD-ORIENTED E-CATALOGUE-BASED BIOLOGY LEARNING TO IMPROVE STUDENT LEARNING OUTCOMES Utami, DianTri; Dewi, Endah Rita Sulistya; Nugroho, Ary Susatyo
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.863

Abstract

Digitalization transformation in learning refers to the use of information and communication technology to improve the learning and teaching process. In the digital era, teaching materials can be accessed in electronic form. One of the electronic teaching materials that can be used is e-catalog, which is an electronic-based catalog innovation, which is easier to learn anytime and anywhere, cheap and easy to share. However, teaching materials used in learning biology in high school are mostly textbooks that have not been digitized. Various environmental problems that currently arise are not entirely derived from the development of the industrial and technological sectors, but the weak level of human literacy, including attitudes and behaviors towards the environment. This causes the relationship between humans and the environment to be less dynamic. Good environmental education can help raise public awareness about the importance of protecting the environment. Education for Sustainable Development (ESD) is considered necessary to be integrated into the curriculum content and implemented into learning. The purpose of this study is to determine the implementation and effectiveness of ESD-oriented e-catalog-based learning digitalization transformation on the concept of environmental change can improve student learning outcomes. This research method uses a Research and Development (R&D) approach, adapting the ADDIE development model. The ESD-oriented e-catalog on the concept of environmental change has a validity value of 96.40% with a very valid category. The practicality value is 94.56% with a very practical category. The effectiveness value of the N-Gain score for cognitive learning outcomes is 75.12% with a fairly effective category. This ESD-oriented e-catalog can be implemented by being used as a comparison and reference material in developing innovative teaching materials to prepare students for the 21st century.
IDENTIFICATION OF PTERIDOPHYTES DIVERSITY IN THE MOUNTAINOUS AREA OF BUNTU SARONG VILLAGE, MASALLE DISTRICT, ENREKANG REGENCY Hasmiati, Hasmiati; Rukmana, Musma; Agustina, Tika Putri; Watung, Fernando Andre
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.1010

Abstract

Ferns (Pteridophyte) are members of the kingdom plantae that contribute to Indonesia's biodiversity, especially the diversity of flora. Ferns have an important role in maintaining the balance of the ecosystem. In addition to having ecological value in the ecosystem as a source of germplasm, ferns also have the potential to be useful to support people's lives. However, in fact ferns are still less well-known and receive less attention than other plants. This is because one of the reasons is that people are less familiar with ferns. This study aims to identify the types of ferns (Pteridophyte) in the mountainous area of ​​Buntu Sarong Village, Masalle District, Enrekang Regency. Data collection was carried out using the exploration method at certain points in the research location that were considered potential, then recording, documenting, identifying and classifying the Pterydophyta found. The research at the research location successfully identified and documented 16 species of Pteridophyte, namely Psilotum nudum, Lycopodium, Selaginella delicatula, Equisetum hyemale, Odontosoria chinensis, Gleichenia linearis, Adiantum sp., Christella parasitica, Aspelinum nidus, Cyathea cooperi, Angiopteris evecta, Deparia petersenii, Microsorum punctatum, Drynaria quercifolia, Microsorum punctatum, Microsorum scolopendria, Drymoglossum piloselloides. The identified Pteridophyte are classified into 13 families (Lycopodiaceae, Psilotaceae, Equisetaceae, Marattiaceae, Lindsaceae, Gleicheniaceae, Adiantaceae, Thelypteridaceae, Asplenidaceae, Chyatheaceae, Athyriaceae, Polypodiaceae) and 5 classes (Lycopodiopsida, Psilotopsida, Equisetopsida, Marattiopsida, Polypodiopsida).
INNOVATION OF MUSHROOM NUGGETS FORTIFIED MORINGA LEAVES AS ANTIOXIDANT SOURCE Pangastuti, Kharisma Diani; Khastini, Rida Oktorida; Hendriyani, Mila Ermila; Murni, Dewi
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.1085

Abstract

This study aims to determine the effect of the type of mushroom on the product quality of mushroom nuggets fortified with moringa leaves with organoleptic and chemical parameters and to determine the level of antioxidant activity. This study used a completely randomized design (CRD) with one treatment factor (type of mushroom). The mushrooms used are oyster mushrooms, straw mushrooms, and enoki mushrooms. The result showed that all samples of mushroom nuggets were acceptable to the panelists, with levels of preference ranging from neutral to favorable. Mushroom nuggets fortified with Moringa leaves have a protein content of 14.90-15.51% and a fat content of 8.78-9.68%. The results of the data analysis showed that the control nuggets were significantly different from all the nuggets treated with different types of mushrooms in the parameters of aroma, taste, and texture. However, the treatment of the nuggets with different types of mushrooms did not show significant differences in the parameters of aroma, taste, and texture. The effects of different types of mushrooms on the protein and fat content of the nuggets were not significant (p > 0.05). The IC50 value of the antioxidant activity of mushroom nuggets fortified with Moringa leaves ranged from 5,500 ppm–34,547 ppm, with a very active category.
ECO-ENZYME : LIQUID ORGANIC FERTILIZER ON THE GROWTH OF MUSTRAIN PLANTS (Brassica chinensis) AS SUPPORTING LITERACY AND NUMERATION Tuapattinaya, Prelly M. J; Tiven, Jessica Gabriella; Fretes, Gracia de; Madubun, Gloria; Lopulalan, Gabriela
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 2 (2024): October 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i2.1098

Abstract

Eco-enzyme is a mixture of organic fruit peel waste fermented with a mixture of brown sugar and water. It can be used as organic fertilizer. The aim of the research is to find out how eco-enzymes help literacy and numeracy in the growth of mustard greens (Brassica chinensis). Eco-enzymes are made with a composition of 10:3:1: 10 liters of water, 3 kilograms of vegetable waste or fruit peels, and 1 kilogram of brown sugar. In a fifteen liter plastic container, dissolve brown sugar in water. Then, add the skins and vegetable waste, and cover tightly and ferment for three months. Mustard seeds are sown and planted in polybags. Watering is done with water every day; watering with eco-enzyme solution is carried out once every three days. The amount of eco-enzyme used is 0 ml, 7.5 ml, 15 ml, and 22.5 ml in one liter of water. The parameters measured for mustard plants are height (cm) and number of leaves (sheets). The research results showed that the application of eco-enzymes as organic fertilizer at a concentration of 7.5 ml had a positive impact on the height and number of leaves of mustard plants, but had a negative impact on eco-enzyme concentrations of 15 ml and 22.5 ml. Thus, it can be concluded that administering eco-enzymes as organic fertilizer provides the most effective effect at the level of 7.5 ml

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