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Lussana Rossita Dewi
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Bioma : Jurnal Ilmiah Biologi
Published by Universitas PGRI Semarang
ISSN : 20865481     EISSN : 25499890     DOI : https://doi.org/10.26877
Core Subject : Science, Education, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Education Immunology & microbiology
Jurnal Ilmiah Biologi focuses on the publication of result’s research related with the major of biology and biology education. For the major of biology, including systematics, physiology and metabolism, ecology, bioconservation, cell and developmental biology, microbiology, genetics, molecular biology, biotechnology, and bioinformatics. For the major of biology education, including biology curriculum, teaching biology, instructional media, and assessment.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 63 Documents
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DEVELOPMENT OF SCIENCE LEARNING DEVICES ORIENTED ESD ON ENVIRONMENTAL POLLUTION MATERIALS TO IMPROVE STUDENTS' CRITICAL THINKING SKILLS AND ENVIRONMENTAL LITERACY Tahmid; khoiri, Nur; Hayat, M. Syaiful
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.823

Abstract

This research aims to develop Education for Sustainable Development (ESD)-oriented science learning tools on environmental pollution material to improve critical thinking skills and environmental literacy for junior high school students. This research is development research (R&D) using a 4D model which includes the define, design, develop and disseminate stages. The tools developed consist of Teaching Modules, Student Worksheets, learning media, and evaluation instruments. Expert validation results show the device is feasible with an average score of 4.2/5. In a limited trial at SMP Negeri 2 Bojong Tegal, the implementation of the Teaching Module was 89%, 81% of students were actively involved, 87% gave positive responses. There was an increase in students' critical thinking skills (average 80.2) and environmental literacy (average 84.5). Success was supported by the application of the ESD approach with project learning, case studies, group discussions, and contextual-interactive media. Implementation of the Independent Curriculum with Teaching Modules and ATP increases student learning independence. The constraints of limited time and inadequate facilities were overcome by efficient time management and teacher creativity. Overall, the tools are valid, practical, effective, in accordance with the Independent Curriculum and 21st century learning with high-level thinking skills, environmental literacy and learning technology.
INDUCTION OF DROUGHT RESISTANCE IN MELON (Cucumis melo L.) M15 WITH HORMOPRIMING BRASSINOSTEROID BASED ON MORFOLOGY, ANATOMY, AND PHYSIOLOGY ASPECTS Rifki, Arif Nur Rifki; Solichatun; Pitoyo, Ari
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.893

Abstract

Melon productivity in Indonesia has been declining. Global climate change is affecting the productivity of melons. Long-term droughts have contributed to a reduction in plant growth and development. Hormopriming is an alternative to increase the germination and growth of melon plants by soaking seeds in a solution of plant growth regulators. Brassinosteroid can enhance germination and plant tolerance under drought conditions. The objective of this research is to examine the impact of brassinosteroid hormopriming treatment on the germination and growth of melon plants subjected to various degrees of drought stress. The study utilized a two-factor fully randomized design. The brassinosteroid concentrations used were 0, 0.05, 0.10, and 0.15 ppm. Media with different water capacities of 75%, 50%, and 25% Space Capacity (SC) were used to test drought stress resistance. Water capacities were examined using the gravimetric method. This study's findings suggest that treating melon plants with brassinosteroid variations can induce drought resistance in M15 melons. A brassinosteroid concentration of 0.15 ppm is the best concentration, as it can increase all parameters of plant growth and adaptation under each variation of water availability provided. The findings of this study can serve as a reference for melon cultivators constrained by dry land conditions to increase the efficiency of cultivation.
OPTIMIZING FIXATION DURATION FOR ENHANCED CLARITY IN Pediculus humanus-capitis WHOLE MOUNT PREPARATIONS USING 10% KOH AT 70°C agustina, Tika putri; Rukmana, Musma; Hasmiati; Watung, Fernando Andre
BIOMA : Jurnal Ilmiah Biologi Vol. 13 No. 1 (2024): April 2024
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/bioma.v13i1.1041

Abstract

This study evaluates the optimal fixation duration using 10% potassium hydroxide (KOH) at 70°C for whole mount preparations of Pediculus humanus capitis (head lice). Accurate head lice identification is crucial for effective public health management. Specimens were fixed for 20, 25, 30, 35, and 40 minutes at 70°C, then processed through rinsing, acetic acid treatment, dehydration, and clearing before mounting for microscopic examination. Results showed that 25 minutes of fixation provided the clearest and most detailed preparations. This duration effectively achieved tissue dehydration and clarification without significant damage. Fixation for 20 minutes was insufficient, resulting in opaque specimens due to incomplete KOH diffusion. Conversely, 40 minutes of fixation caused over-fixation, leading to tissue damage and poorer color quality. The study underscores the importance of finding the right fixation duration. While shorter times are inadequate, longer durations can degrade specimen quality. A 25-minute fixation at 70°C is optimal for balancing clarity and structural preservation, improving head lice identification. This method enhances the quality of whole mount preparations and provides practical guidance for accurate and reliable ectoparasite examination in clinical and research settings.
ETHNOBOTANICAL STUDY OF RAJABIYAH TRADITION Nurul Mufidah, Elyza Rohman; Darmawan, Ericka; Prajoko, Setiyo
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/8npfcq21

Abstract

Indonesia is a country with diverse tribes, cultures, and customs in each region. The Rajabiyah Tradition is a local culture of the Prawoto Village community in Pati Regency, the implementation of which is related to the use of surrounding plants, both for traditional rituals and religious rituals. This study aims to determine the ethnobotanical profile of the Rajabiyah Tradition which includes the istighosah procession, grave pilgrimage, buka luwur, washing of grave banners, luwur procession and cultural procession, and tabligh akbar. This study is a combined study, namely ethnographic research with a qualitative descriptive and quantitative descriptive research approach. Samples were taken using snowball sampling and purposive sampling techniques. Data collection was carried out through semi-structured interviews. Interviews were conducted with 30 informants who were 1 village official, 1 traditional leader, 1 grave caretaker, and 1 community leader and 26 residents of Prawoto Village. The results of the study obtained 49 types of plants used in each Rajabiyah Tradition procession which were grouped into 26 diversities of order levels. The most widely used order is the Zingiberales order and the most widely used part of the plant is the fruit. Each plant used has a different aspect of meaning which is the good hope of the people of Prawoto Village.
IMPACT OF THE STUDENT FACILITATOR AND EXPLAINING MODEL INTEGRATED WITH SOCIO-SCIENTIFIC ISSUES ON ANALYTICAL THINKING SKILLS IN BIOLOGY EDUCATION Maryanti; Wicaksana, Ervan J; Mursyd, Danial `
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/sy0vtt35

Abstract

The Merdeka Curriculum is designed to transform education by enhancing student engagement and preparing learners to face the complexities of the modern era. However, observations at SMA Negeri 9 revealed that students still struggle to connect information from various sources and show low participation in class discussions. This study aimed to examine the effect of the Student Facilitator and Explaining (SFE) model integrated with Socio-Scientific Issues (SSI) on students’ analytical thinking in biology learning. The research used a quasi-experimental method with a non-randomized control group pretest-posttest design. Samples were selected using purposive sampling, consisting of an experimental class applying the SFE-SSI model and a control class using Problem-Based Learning (PBL). Data were collected through essay tests and observation sheets, and then analyzed using One-Way ANCOVA. The results showed a significant effect of the learning model on analytical thinking [F(1,69) = 12.245, p < 0.001, ηp² = 0.151]. It is concluded that the SFE model integrated with SSI effectively enhances students’ analytical thinking in biology learning.
STUDY OF THE EFFECTIVENESS OF ENVIRONMENTAL ENRICHMENT ON LONG-TAILED MONKEYS (Macaca fascicularis) WITH ABNORMAL BEHAVIOR Zaman, Syifa Faujiah; Siagian, Tetty Barunawati; Saputro, Suryo
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/ec91sa91

Abstract

Macaca fascicularis merupakan salah satu jenis primata penghuni hutan yang memiliki arti penting bagi kehidupan di alam. Pemeliharaan Macaca fascicularis secara eks situ dalam kandang individu sering kali memicu perilaku abnormal pada Macaca fascicularis. Oleh karena itu, diperlukan pengayaan lingkungan untuk meminimalisir perilaku abnormal tersebut. Penelitian ini bertujuan untuk mengidentifikasi efektivitas program pengayaan ekologi dalam menurunkan perilaku abnormal pada Macaca fascicularis. Pengamatan perilaku dilakukan terhadap 4 ekor Macaca fascicularis yang berperilaku abnormal dengan jumlah jantan 3 ekor dan betina 1 ekor menggunakan metode focal animal sampling. Pengamatan perilaku dimulai tanpa pengayaan, kemudian diberikan perlakuan pengayaan berupa puzzle ball, dan feeding tube yang berisi wortel dan kacang panjang. Setiap pengamatan dilakukan selama 2 minggu, dengan frekuensi pengamatan sebanyak 2 kali sehari selama 30 menit. Hasil penelitian menunjukkan bahwa perilaku dominan Macaca fascicularis sebelum diberikan pengayaan, menunjukkan perilaku abnormal, istirahat, dan agonistik. Perilaku setelah diberikan pengayaan puzzle ball dan feeding tube yaitu mencari makan, makan, dan perilaku abnormal. Efektivitas kedua pengayaan tersebut dibuktikan dengan uji repeated measure ANOVA yang memiliki hasil (Sig. 0,007 < 0,05). Artinya terdapat perbedaan rata-rata yang signifikan dalam mengurangi perilaku abnormal pada Macaca fascicularis. Berdasarkan hasil penelitian, pengayaan lingkungan dapat membantu mengurangi perilaku abnormal dan meningkatkan kesejahteraan Macaca fascicularis di penangkaran.
THE FEASIBILITY OF HEYZINE-BASED E-FLIPBOOK MEDIA FOR KINGDOM MONERA SUB-MATERIAL Annisa Nabilah Mufidah; Eko Sri Wahyuni; Laili Fitri Yeni
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/207e2356

Abstract

E-flipbook media is one of the audio-visual media presented in HTML format. It can be presented by using the online application service, Heyzine. The e-flipbook presents the results of the antibacterial test of thorny bajakah stem extract (Caesalpinia sumatrana Roxb.) against Salmonella typhi and its related to the kingdom monera sub-material in the tenth grade senior high school. The research was conducted as descriptive quantitative with the presentation of data from the analysis of media feasibility. The data of media feasibility was collected in the form of a validation sheet conducted by 5 validators using a Likert scale with 5 aspects of validation, namely format, content, language, practicality, and effectiveness of the media. The feasibility test results show an average Aiken’s V value of 0.88 valid category with an ICC value of 0.863 very good category. Based on the results of this research, it can be concluded that e-flipbook is suitable for use as a learning media in schools.
BUILDING SUSTAINABILITY AWARENESS: INTEGRATING ESD INTO HUMAN DIGESTIVE SYSTEM EDUCATION Setiani, Rina; M. Syaipul Hayat; Roshayanti, Fenny; Sumarno
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/fy85sf26

Abstract

In response to global environmental challenges such as climate change, pollution, and resource depletion, this study aimed to develop and evaluate Education for Sustainable Development (ESD)–oriented science learning materials on the human digestive system to enhance students’ sustainability awareness. Employing a Research and Development (R&D) approach and the ADDIE model, the instructional materials were implemented in a quasi‑experimental design with two eighth‑grade groups at SMP Negeri 2 Tulis during the 2024/2025 odd semester. Sustainability awareness was measured using a 26‑item questionnaire administered as a pretest and posttest. The experimental group, which received the ESD‑oriented intervention, demonstrated a mean pretest score of 70.55 and a posttest score of 92.06 (mean N‑Gain = 0.63). The experimental group showed significantly higher gains in both sustainability awareness—especially in emotional engagement—compared to the control group. These outcomes suggest that integrating ESD into science learning not only improves conceptual understanding but also shaping environmentally responsible and reflective students.
IN SILICO ANALYSIS ON BINDING ACTION OF TERPENE NATURAL COMPOUNDS FROM APOCYNACEAE FAMILY AGAINST SHV-1 BETA-LACTAMASE FROM Klebsiella pneumoniae A M Sabbah, Sajida; Indri Astuti, Rika; Budiarti, Sri
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/585f2k97

Abstract

Antibiotic resistance is a growing threat to public health, particularly with hospital-acquired infections caused by Klebsiella pneumoniae. The limited treatment options for these infections are further complicated by the emergence of antibiotic-resistant strains due to the overuse of antibiotics. This study investigates the potential of Monoterpene Indole Alkaloids (MIAs) derived from six genera within the Apocynaceae family as inhibitors of the beta-lactamase enzyme (bla-SHV-1), which plays a key role in antibiotic resistance in Klebsiella pneumoniae. Using the PyRx program for molecular docking, we assessed the binding affinity and interaction profiles of various MIAs with bla-SHV-1. Our results identified Paucidisine, (-)-19-Oxoisoeburnamine, and Paucidactine A as the most promising candidates, based on their interaction energies and binding modes. Detailed analysis of ligand-protein interactions, including hydrogen bonds and hydrophobic interactions, revealed that these MIAs exhibit higher binding affinity and structural similarity to the native ligand bla-SHV-1. While these findings are promising, it is important to note that they are based on in silico models. Further in vitro and in vivo studies are required to validate the efficacy and safety of these MIAs as potential therapeutic agents. This research marks a significant step towards developing alternative treatment options for antibiotic-resistant Klebsiella infections, potentially improving patient outcomes and addressing the global challenge of antibiotic resistance. In conclusion, our study offers novel insights into the potential use of MIAs as inhibitors of bla-SHV-1, contributing to the ongoing efforts to combat antibiotic resistance. Future research should focus on validating these in silico results through rigorous experimental and clinical studies, considering the ethical implications of introducing new treatments.
POTENTIAL IMPLEMENTATION OF ETHNOSCIENCE-ORIENTED LEARNING ON BIOTECHNOLOGY MATERIAL Anisak, Hijjriyah; Patonah, Siti; `Hayat, M.Syaipul
BIOMA : Jurnal Ilmiah Biologi Vol. 14 No. 2 (2025): October 2025
Publisher : Prodi Pendidikan Biologi, FPMIPATI, Universitas PGRI Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.26877/h1m2ct96

Abstract

There are still many science learning practices that focus on memorization and do not relate the subject matter to real life or the local cultural context of students. Science is often perceived as a difficult and boring subject because it relies heavily on technical terms and abstract material. This study aims to         explore the potential implementation of     ethnoscience oriented learning in biotechnology material at the junior high school level. Data were collected through interviews with 11 science teachers from Sub Rayon 05 of Grobogan Regency and classroom observations involving 3 peer teachers at SMP Negeri 1 Ngaringan. The        interview was conducted using a Google Form-based questionnaire, while observation data were obtained through direct observation using a structured     observation sheet. The results showed that teachers had a good understanding of ethnoscience (78.7%), and the potential for    implementation was considered very high (84%). However, curriculum development remained the lowest aspect observed (67%). These findings indicate that while the conceptual understanding and enthusiasm of teachers toward ethnoscience are strong, effective implementation           requires systemic support, training, and curricular alignment.

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