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Life Science
Published by Universitas Negeri Semarang
ISSN : -     EISSN : 25285009     DOI : https://doi.org/10.15294/unnesjlifesci
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Life Science published original and significant articles on all aspects of Life Sciences (Biology, Genetics, Biological Anthropology, Botany, Medical Sciences, Veterinary Sciences, Biochemical Genetics, Biometry, Clinical Genetics, Cytogenetics, Genetic Epidemiology, Genetic Testing, Evolution and Population Genetics, Immunogenetics and Molecular Genetics). Starting from Vol. 14 (1) 2025, all submitted manuscripts must be in English and include Turnitin and AI plagiarism scan results. Authors are also required to attach ethical clearance files from relevant official institutions for research using human and animal samples.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 30 Documents
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Potensi Cadangan Karbon Tersimpan dan Serapan Karbon pada Ekosistem Hutan Kota Tinjomoyo Semarang Nur Puji Lestari; Nana Kariada Tri Martuti
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.592

Abstract

The increase in GHGs in Semarang City, caused by the increase in motorized vehicles and industrial activity, has resulted in a decrease in environmental quality in urban areas and air pollution. Data from the Semarang City Environmental Service (2019), shows that total CO2e emissions in 2018 were dominated by CO2, namely 33,505,357.22 tons of CO2 (85.24%) of the total CO2e tons of GHG emissions. To reduce CO2 emissions in Semarang City, one way that can be done is by utilizing urban forests which can absorb carbon dioxide. Based on these conditions, estimates of stored carbon reserves and carbon uptake in urban forests need to be calculated to determine the CO2 that plants in urban forests can absorb. This research aims to analyze the amount of stored carbon reserves and carbon uptake in the Tinjomoyo Semarang City Forest ecosystem. This research uses an exploratory method carried out through direct observation and measurement in the field. The sampling technique used is the belt transect method. The stand measurement method is carried out using the Non-Destructive Test (NDT) method or by not damaging the research samples. Stand data analysis was carried out using the allometric equation method. Based on calculation results, the number of individual stands in the Tinjomoyo Semarang City Forest area is 367 individual stands. The total amount of biomass is 125.5 tonnes/ha, the total carbon stock is 57.72 tonnes/ha and the total amount of carbon uptake is 211.8 tonnes/ha. This research shows that the estimated carbon reserves stored in the Tinjomoyo City Forest ecosystem, Semarang are 57.72 tonnes/ha with a carbon uptake value of 211.8 tonnes/ha.
Aktivitas Antibakteri Ekstrak Buah Asam Kalimbawan (Sarcotheca diversifolia (Miq) Hallier F) terhadap Bakteri Staphylococcus aureus dan Escherichia coli Indah Pratiwi; Rikhsan Kurniatuhadi; Rahmawati
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.1312

Abstract

Asam kalimbawan (Sarcotheca diversifolia (Miq) Hallier F) is a native plant found in Kalimantan, especially in West Kalimantan. This species belongs to the Oxalidaceae family, known for producing compounds with antioxidant and anti-inflammatory properties. This study aims to investigate the antibacterial activity of kalimbawan tamarind fruit extract against Staphylococcus aureus and Escherichia coli bacteria. Fruit extraction was carried out using the maceration method with methanol solvent, and the antibacterial activity was performed using the disc diffusion method. This research utilized a Completely Randomized Design (CRD) with seven treatment levels and four replications for each bacterium. The test concentrations of the extract against S. aureus and E. coli were 0.1, 0.2, 0.3, 0.4, and 0.5 g/mL, as well as ciprofloxacin and 10% DMSO. The study results revealed that all concentrations of the extract were able to inhibit the growth of the test bacteria, as indicated by the presence of a clear zone. The effective extract concentrations against S. aureus and E. coli bacteria were 0.4 g/mL and 0.3 g/mL, respectively, with corresponding zone diameters of 16.55 mm and 14.22 mm, respectively. Asam kalimbawan, which has been traditionally used by the people of Sambas Regency, has the potential to be developed as a source of natural metabolite-derived medicines.
Kepadatan Bakteri Biofertilizer Cair Air Kelapa Menggunakan Bakteri Pelarut Fosfat (Bacillus spp.) Asal Tanah Gambut Kalimantan Barat: Density of Liquid Biofertilizer Coconut Water Bacteria Using Phosphate Solubilizing Bacteria (Bacillus spp.) from West Kalimantan Peat Soil Sari, Lala; Rahmawati; Khotimah, Siti; Mukarlina
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.1673

Abstract

Biofertilizer is an environmentally friendly fertilizer, has a long release time so it can be absorbed by plants long enough, and has enzymatic activities such as binding nitrogen and dissolving phosphate. Phosphate solubilizing bacteria (BPF) can dissolve phosphate that was previously unavailable to become available. This research aims to further examine the potential of BPF isolates from Kalimantan as a liquid biofertilizer agent using coconut water as a medium. The research was conducted for 3 months, from June to August 2023 at the Microbiology Laboratory, Biology Department, Faculty of Mathematics and Natural Science Tanjungpura University, Pontianak. This research used a Completely Randomized Design (CRD) with 8 treatments and 3 replications using the Total Plate Count method. The results of the study showed that the highest treatment is a treatment of A4 on day 6 with an average density of 9,4x108 CFU/mL. The BPF which has the highest potential as a biofertilizer is found in treatment A4, the combination bacterial isolates of Bacillus sp1 and Bacillus sp2, supported by the high population treatment of A4 during each storage. Liquid biofertilizers have many advantages compared to solid biofertilizers, including having a higher number of microbial cells, longer shelf life, greater protection against the environment and better effectiveness in the field. This study can provide information on the density of liquid biofertilizer bacteria using environmentally friendly coconut water media for the community by using phosphate-solubilizing bacteria (Bacillus spp.) from West Kalimantan peat soil.
Potensi Bakteri Pelarut Fosfat dan Bakteri Penghasil IAA Asal Tanah Gambut terhadap Perkecambahan  Tanaman Jagung (Zea mays L.) Anggita, Via; Khotimah, Siti; Rahmawati; Mukarlina
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.1729

Abstract

Phosphate solubilizing bacteria and IAA-producing bacteria are bacteria that can help plant growth through their use by increasing the availability of phosphate (P) in the soil and helping plants absorb nutrients in the soil. This study aims to determine the effect of phosphate solubilizing bacteria and IAA-producing bacteria isolated from peat soil on the germination of corn (Zea mays L.) plants grown on peat soil.  This research was conducted from June to September 2023 with a completely randomized design (CRD). Testing the effect of phosphate-solubilizing and IAA-producing bacteria was carried out using the method of soaking seeds and watering bacterial suspensions on plants.  The results showed that A5 treatment had a significant effect on the number of leaves parameter, with A5 treatment (Bacillus cereus PF12, P13 and PF16 + Pseudomonas stutzeriIAA1) producing a germination percentage (100%), the number of leaves (5.09 strands) while the parameters of plant height and root length showed that all treatments were not significantly different.    
Potensi Pemberian Bakteri Penghasil IAA (Pseudomonas sp. IAA1 dan Bacillus sp. IAA2) terhadap Perkecambahan Zea mays saccharata Sturt.: Potential of Giving IAA Producing Bacteria (Pseudomonas sp. IAA1 and Bacillus sp. IAA2) on Germination of Zea mays saccharata Sturt. Andini Widyaningsih; Siti Khotimah; Rahmawati
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.2214

Abstract

Indigenous peatland bacteria producing indole acetic acid (IAA) hormone function to help in the physiological process of plants during vegetative growth of plants. This study aims to determine the effect of giving IAA-producing bacteria (Pseudomonas sp. IAA1 and Bacillus sp. IAA2) and to determine the best density of IAA-producing bacteria for sweet corn germination (Zea mays saccharata Sturt). This study used a completely randomized design (CRD), with 8 treatment levels and 3 replications. The research data were analyzed using Ms. Excel by finding the average germination time. The results showed that the provision of IAA-producing bacteria had an effect on the germination time of sweet corn plants. The treatment of giving Bacillus sp. IAA2 bacteria with a bacterial density of 1.04 × 107 cells/mL gave the best results in the germination time of sweet corn with an average germination time of 2.00 days, while Pseudomonas sp. IAA1 has a bacterial density of 1.51 × 107 cells/mL and an average sweet corn germination time of 2.25 days. The bacterial consortium of Pseudomonas sp. IAA 1 and Bacillus sp. IAA2 has a bacterial cell density of 2.04 cells/mL and an average sweet corn germination time of 2.30 days. These indigenous bacteria producing IAA can be developed into biofertilizers so that they can replace chemical fertilizers that can harm the environment, especially the soil.
Pengaruh Variasi Metode Ekstraksi Metabolit Sekunder Bakteri Endofit Daun Rambutan (Nephelium lappaceum L.) terhadap Pertumbuhan Escherichia coli dan Bacillus subtilis Hasyim Abdurrasyid; Ibnul Mubarok; Dewi Mustikaningtyas; Pramesti Dewi
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.2327

Abstract

Rambutan (Nephelium lappaceum L.) is one of the many tropical plants found in Indonesia. The leaves of the rambutan plant are known to be used as medicine, so they have the potential to be a source of endophytic bacteria that can produce beneficial compound. This study aims to isolate, identify of endophytic bacteria from rambutan leaves, and test their activity as an antibacterial with two different secondary metabolite extraction methods, namely maceration with 70% methanol and without maceration. This study used experimental research with a Completely Ramdomized Design (CRD). Isolation of endophytic bacteria from rambutan leaves produced two isolates (D1 and D2). The results of identification through macroscopic, microscopic, and biochemical test showed that two pure isolates had similarities to genus Bacillus sp. Antibacterial activity testing was carried out using well diffusion method. Based on the results of antibacterial test showed that extraction method with 70% methanol produced an extract that was more effective as an antibacterial with average diameter of clear zone formed for Escherichia coli bacteria were 13.55 mm (Isolate D1) and 12.13 mm (Isolate D2) and that of Bacillus subtilis bacteria were 16.27 mm (Isolate D1) and 19.69 mm (Isolate D2). This finding contributes to the fact that the type of secondary metabolite extraction method of rambutan leaf endophytic bacteria influences the sensivity as an antibacterial.
Tingkat Pemanfaatan Teripang Ekonomis Penting di Perairan Kepulauan Karimunjawa Amalo, Nancy Marcelina Reinatha; Muskananfola, Max Rudolf; Rudiyanti, Siti
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.4417

Abstract

Sea cucumbers are an economically important fishery commodity in great demand by both local and international audiences. As time goes by, the need for sea cucumbers increases and people make excessive use of sea cucumber resources until they reach the status of over-exploitation. This makes it necessary to know the level of utilization of sea cucumbers in Karimunjawa. This research was carried out on August - September 2023 in Tanjung Gelam, Karimunjawa. The purpose of this research is to find out the catch per unit effort, maximum sustainable potential/maximum sustainable yield, and the level of utilization of sea cucumbers in Karimunjawa waters. This research uses a survey method with the survey carried out to collect data regarding catches and fishing trips for fishermen directly. The data analysis method uses model calculations Schaefer. From the research results, it was found that the average CPUE production value was 4 kg/trip. The potential MSY value obtained is 2.857 kg/year with an optimum effort (f opt) of 620 trips/year. The level of utilization of sea cucumbers has an average value of 93,27% which is categorized as optimum, so there is no need to make additional efforts because the catch is already optimal and if it is added it will cause an excess catch.  
Pengaruh Lama Penyimpanan Biji Kopi Liberika pada Suhu Kamar terhadap Mutu Mikrobiologis dan Kadar Okratoksin A: The Effect of Storage Time of Liberica Coffee Beans at Room Temperature on Microbiological Quality and Ochratoxin A Levels Ramadani, Mutiara Bintang; Dewi Mustikaningtyas; Siti Harnina Bintari; Pramesti Dewi
Life Science Vol. 14 No. 1 (2025): May 2025
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v14.i1.5810

Abstract

Liberica coffee is one of the leading commodities in several regions so it has quite high economic potential. Coffee beans can be stored for quite a long time, more than one year, even more than 4 years before going through the roasting process. However, during long storage periods coffee beans can be attacked by microorganisms such as mold (ochratoxigenic and non-ochratoxigenic). This research aims to determine the effect of storage time on the quality of Liberica coffee beans, both microbiologically and chemically. The benefit of this research is to provide information on the quality of Liberica coffee beans. This research is a laboratory experimental research. The long storage time for coffee beans was 36 and 39 weeks. The results of the research showed that the yeast mold rate (AKK) on Liberica coffee beans stored for 39 weeks was greater than those stored for 36 weeks. The increase in AKK during storage is thought to be due to the presence of insects, temperature and humidity factors. A total of three mold isolates were found at week 36 and there were six mold isolates at week 39. The percentage of fungal isolate attacks at both weeks 36 and 39 was dominated by fungi of the Mucorales order (Mucor sp. and Absidia sp.). In the 36th week the attack of Mucor sp. amounted to 36.67% while in the 39th week the Mucor sp attack. 20%. and Absidia sp. 23.33%. Ochratoxin A (OTA) levels from week 36 to week 39 did not increase but instead decreased. This occurs due to OTA degradation by ochratoxin-degrading molds which are thought to be Aspergillus sp. during storage.
Keanekaragaman Serangga dan Tingkat Kerusakan Akibat Serangan Hama pada Tanaman Jeruk Melinda, Aura Tirsa; Dyah Rini Indriyanti; Priyantini Widiyaningrum; Niken Subekti
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.10758

Abstract

Citrus are a horticultural commodity that has high economic value and tends to be liked by many people. In the Bawen area, Semarang Regency, there is a plantation that cultivates various types of orange plants. Many citrus plants have signs of pest attacks that have not yet been identified. This research aims to obtain data related to insect diversity and the level of damage caused by insect pest attacks on citrus plants. This research is an exploratory type of research, using a survey method. The research location was determined using purposive sampling by taking three fields of Siam Honey, Sumowono and Pamello oranges. Insect sampling was carried out using pitfall trap, yellow trap, direct observation and interviews. The results of the study showed that the diversity index was H' = 3.3, honey siam = 3.3, pamello orange (H' = 3.1) and sumowono siam (H' = 2.9). The similarity value of the three types of orange is 0.8 and the dominance is 0.05 (honey siamese), 0.06 (pamello) and 0.07 (sumowono). At the time of research, orange plants were in the generative phase so they could attract more insects. The level of damage due to pest attacks is classified as moderate. The conclusion of this research is that the highest species diversity value is Siam Honey, then Pamello orange and Siam Sumowono, the presence of insects is evenly distributed and no one species dominates. The benefit of this research is to obtain information regarding the diversity of insect types and the level of damage caused by insect pest attacks.
Estimasi Populasi dan Habitat Monyet Ekor Panjang (Macaca fascicularis Raffles, 1821) di Desa Ngesrepbalong, Jawa Tengah S, Christine Nadya; Rahayuningsih, Margareta
Life Science Vol. 13 No. 2 (2024): November 2024
Publisher : Universitas Negeri Semarang

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.15294/unnesjlifesci.v13.i2.11787

Abstract

Mount Ungaran leaves an important ecosystem as a habitat for various species, including long-tailed macaques (Macaca fascicularis Raffles, 1821). Long-tailed macaques (MEP) are a type of animal that is not protected according to PP No. 106 of 2018. In the IUCN, the conservation status of MEP has changed from the vulnerable category to endangered and is included in Appendix II based on CITES. Estimation of the population of a species is very necessary to provide an overview of the existence of the species in an area. There has been no research on the estimation of the population and habitat of MEP in Ngesrepbalong Village. The purpose of this study was to analyze the estimation of the MEP population and the condition of the MEP habitat in Ngesrepbalong Village, Central Java. The method used in this study was the survey method with the calculation of concentration points (concentration count). This method is used on various types of wild animals that gather in groups at observation points that have been previously determined based on the results of preliminary surveys along with the habitat conditions analyzed to produce an estimate of the MEP population and habitat at the research location. The results of the analysis of the estimated MEP population in Ngesrepbalong Village found 53 individuals in two groups at two observation points, namely Gunungsari and Separe with the percentage of the MEP population based on sex, namely 28% juveniles, 38% juveniles, and 34% adults. The increasing number of young MEP individuals indicates that the population will increase assuming a constant mortality rate. Poaching and habitat carrying capacity are the most influential factors in the difference in population numbers at the two observation points. The highest MEP encounters were at the Gunungsari observation point.

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