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Journal of Artha Biological Engineering
Published by PT. Artha GenetikaLab Indonesia
ISSN : 30464889     EISSN : 30324394     DOI : https://doi.org/10.62521/pm6jjq40
Core Subject : Health, Science, Agriculture, Social, Engineering,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Chemical Engineering, Chemistry & Bioengineering Environmental Science Medicine & Pharmacology
The scope of this journal is to publish original research papers, research notes, and reviews in the field of Biological Engineering widely. As such, high quality research papers or reviews dealing with any aspect of Biological Engineering are welcome. Papers that are theoretical, interpretive or experimental.
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 30 Documents
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Effectiveness of Minaraya Probiotics on The Growth Rate and Immune Response of Catfish (Clarias gariepinus) ren fitriadi; Purnama Sukardi; reza Muhammad Riady; mohmammad nurhafid; Agnes Heratri; Maghfira Selia Irawati
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/bdxbgh67

Abstract

The growing demand for catfish in the market is prompting farmers to consistently enhance their production capacity. Several obstacles are encountered during production growth, such as pathogen attacks, suboptimal growth, and low survival rates. This study aimed to determine the effectiveness of Minaraya probiotics with different doses in feed on the growth and immune response of catfish. The method used in this study was an experimental method with a completely randomized design (CRD) consisting of four treatments: control (without probiotics), and treatments P1, P2, and P3 with the addition of Minaraya probiotics at 2, 5, and 10 mL per 2.5 kg of feed, respectively, each with three replications. The parameters observed included survival rate, SGR, DGR, FCR, PER, AF, RB, and leukocyte differentiation. The results showed that the survival rate in all treatments was relatively high, ranging from 95% to 98.89%. The results also showed that the addition of Minaraya probiotics significantly (p<0.05) increased growth parameters (SGR and DGR) and feed efficiency (FCR and PER), with the best dose being in treatment 3 (P3) at a dose of 10 mL/2.5 kg of feed. On the other hand, the non-specific immune response also increased in the treatment given Minaraya probiotics, as indicated by increased phagocytic activity, respiratory burst, and proportion of lymphocytes, neutrophils, and monocytes, as well as an increase in the total number of erythrocytes after Aeromonas hydrophila infection. The application of Minaraya probiotics in a sustainable cultivation system can improve catfish productivity and health.
Screening of Amylolytic Bacteria from the Intestines of Tilapia (Oreochromis niloticus) Cultivated in Rice Fish Farming in Panembangan Village, Cilongok District, Banyumas salsa nabila; Kasprijo; Rima Oktavia Kusuma; Sri Marnani; Muhammad Ikhwan Ihtifazhuddin
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/p5vczh22

Abstract

Amylolytic bacteria in the digestive tract of tilapia are bacteria that produceamylase enzymes, which function to break down carbohydrates, thereby aidingthe digestive process. The presence of amylolytic bacteria is influenced by feedand the environment. The purpose of this study was to determine the proportionand activity index of amylolytic bacteria in the intestines of tilapia. This studyused an observational method with purposive sampling. Amylolytic bacteriawere isolated from the intestines of tilapia in Rice fish farming Blocks A and E.The successfully isolated bacteria were grown on 1% starch agar medium withan incubation time of 48 hours and observed at 24-hour intervals. Then, severalcharacteristic tests were carried out, including Gram staining, KOH, catalase,and oxidase tests. Based on the results of the study, the proportion of amylolyticbacteria was found in 10 isolates with a proportion of 44% and 12 isolates witha proportion of 48% in the intestines of tilapia from the two blocks. The averageamylolytic activity index obtained from block A was 0.96 ± 0.237, and fromblock E was 1.49 ± 0.501. The largest amylolytic activity index was obtainedin isolate KSA 19 with a value of 1.33 from block A, while from block E, thelargest amylolytic activity index value was 2.6 in isolate KSE 5.
Fluctuation of Green Algae and Effects on the Growth of Pacific White Shrimp (Litopenaeus vannamei) Mia Pinandita; Dewi Nugrayani; Agung Cahyo Setyawan; Imam Marzuki
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/tcxv0s65

Abstract

Algae are eukaryotic organisms with chlorophyll. Green algae contain chlorophyll, allowing them to photosynthesize directly and provide shrimp ponds with food. This study is entitled Fluctuations of Green Algae and Effects on the Growth of Pacific White Shrimp (Litopenaeus vannamei) in CV. Cemarasewu Sumber Rejeki, Cilacap. This observational study Oct–Dec 2022 monitored a pond in Cilacap. Water green algae and Pacific White Shrimp samples were taken every seven days age 64–120 days. Algae density was calculated hemocytometer, while shrimp growth parameters MBW, ADG, FCR, SR and water quality temperature, pH, DO, salinity, nutrients were measured. Data were analyzed descriptively to observe the relationship between algae fluctuations and shrimp growth. The results of the study showed that green algae experienced fluctuations during the Pacific White Shrimp cultivation period. The presence of green algae can have a good effect on the growth rate of Pacific White Shrimp. The growth rate of Pacific White Shrimp in the research pond was classified as good. The results obtained by ADG in plot B13 0.39%, B14 0.45% and B15 0.38%. MBW in plot B13 30.75 g, B14 31.68 and B15 32.05 g. FCR in plot B13 1.42, B14 1.45 and B15 1.39 and SR in plot B13 66.7%, B14 71.5% and B15 70.7%.  
Monitoring Of Water Quality In Vanamei Shrimp (Litopenaeus vannamei) Farming In Cv. Cemarasewu Sumber Rejeki, Cilacap Mia Pinandita; Dewi Nugrayani; Imam Marzuki
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/jma13a03

Abstract

Pacific White Shrimp (Litopenaeus vannamei) is a frequently cultivated shrimp species. This is because it offers promising prospects and profits. Superior vannamei can be produced by considering several aspects of the cultivation process. Internal factors include the origin and quality of the seeds, as well as external factors, including cultivation water quality, feeding, technology, and pest and disease control. This research was conducted from September 15 to October 15, 2022, at CV. Cemarasewu Sumber Rejeki, Jetis Village, Cilacap, Central Java. The purpose of this study was to determine the water quality conditions in Pacific White Shrimp rearing ponds at CV. Cemarasewu Sumber Rejeki, Cilacap. Three ponds were used for data collection: plots B13, B14, and B15, with shrimp rearing ages of 100-129 days. Research results indicated that plots B13, B14, and B15 at CV. Cemarasewu Sumber Rejeki provides good water quality for Pacific White Shrimp cultivation. The results of water quality parameters measured in this study include: brightness up to 35 cm, temperature ranging from 27-29 ºC, pH ranging from 7.6-8.3, salinity ranging from 14-22 ppt, alkalinity ranging from 125-169.6 ppm, TOM ranging from 70.4-79.2 ppm, DO ranging from 4.32-4.98 ppm, phosphate ranging from 0.25-0.5 ppm, nitrite ranging from 0.013-0.085 ppm, nitrate 0 ppm, ammonia ranging from 0.00-0.085 ppm, ammonium ranging from 0.00-0.50 ppm and plankton ranging from 2,500-282,500. It can be concluded that the water quality in pond plots B13, B14, and B15 at CV. Cemarasewu Sumber Rejeki meets the appropriate standards to support Pacific white shrimp cultivation.
Proteolytic Bacterial Activity from the Digestive Tract of Udikan Fish (Tor Sp.) Caught in Banjaran River Roni Ruswanda; Anandita Ekasanti; Rima Oktavia Kusuma
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/jmdy2152

Abstract

The type of feed commonly consumed by fish in nature can be predicted from the presence of microorganism communities in their digestive tract. One of the bacteria that plays an important role in the digestive tract is proteolytic bacteria. The purpose of this study was to determine the activity index and proportion of proteolytic bacteria from the digestive tract of udikan fish (Tor sp.) caught in the Banjaran River. The method used in this study was observation with a sampling technique conducted by purposive sampling. The activity index of proteolytic bacteria from the digestive tract of udikan fish (Tor sp.) was relatively low with an average value of 0.06 in 24-hour observations and 0.05 in 48-hour observations. The proportion of proteolytic bacteria obtained from the digestive tract of udikan fish (Tor sp.) was 33%.
The Effect of Shrimp Pond Solid Waste Utilization on Protein and Chlorophyll Content in Caulerpa lentillifera Cultivation Muhammad Ikhwan Ihtifazhuddin; Nyoman Robby Manik Saputra; Ramanda Ahmad Rizal Rifa’i; Priatno Khanna
Journal Of Artha Biological Engineering Vol. 4 No. 1 (2026)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/4m8gx056

Abstract

Aquaculture waste is material produced by fish or shrimp farming that can pollute aquatic environments if not promptly managed. The solution to solve this problem is to utilize shrimp pond solid waste as organic fertilizer. The shrimp pond solid waste will be used as fertilizer to cultivate Caulerpa lentillifera. Caulerpa lentillifera cultivation has begun to develop in Indonesia because, in addition to being edible fresh, it also contains good nutrition for the body. This study aims to determine the effect of shrimp pond solid waste on protein and chlorophyll levels of Caulerpa lentillifera. This study was conducted with 4 (four) treatments in the form of different doses of shrimp pond waste (0, 2, 4, and 6 g/L) and 3 (three) replications in each treatment. The parameters tested included nutrient, protein, and chlorophyll content, as well as water quality, in Caulerpa lentillifera cultivation. The results obtained from this study are Caulerpa lentillifera is able to utilize the nutrient content in shrimp pond solid waste as fertilizer to add nutrients. Treatment C had the highest effect with protein content (8.27%) and chlorophyll-a (3.46 mg/g), chlorophyll-b (7.41 mg/g); and total chlorophyll (16.08 mg/g) in Caulerpa lentillifera. Based on these data, it can be seen that shrimp pond solid waste can be used as fertilizer in the cultivation of Caulerpa lentillifera.
A Anti-Vibrio Activity of Bacteria Isolated from the Intestine of Mantis Shrimp (Harpiosquilla sp.): Aktivitas Anti-Vibrio Bakteri yang Diisolasi dari Usus Udang Mantis (Harpiosquilla sp.) Navita Desy Ratnaningsih; Petrus Hary Tjahja Soedibya; Jefri Anjaini
Journal Of Artha Biological Engineering Vol. 4 No. 1 (2026)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/nc8tjk69

Abstract

Shrimp diseases caused by Vibrio spp. remain a major constraint in aquaculture, necessitating the exploration of alternative antibacterial agents from natural sources. This study aimed to determine the proportion and antibacterial activity of bacterial isolates derived from the intestines of mantis shrimp. Samples were collected from Jepara, Pemalang, and Cilacap waters using a purposive sampling approach. The research methods included bacterial isolation, morphological characterization, and screening of antivibrio activity. Antibacterial activity was evaluated against Vibrio parahaemolyticus, Vibrio alginolyticus, and Vibrio harveyi using dotting and disc diffusion methods. The results showed that 27% of isolates from Jepara, 44% from Pemalang, and 16% from Cilacap exhibited antivibrio activity. The inhibition zones ranged from 1.1–11.0 mm against V. parahaemolyticus, 3.3–9.0 mm against V. alginolyticus, and 2.2–11.8 mm against V. harveyi. These findings indicate that intestinal bacteria of mantis shrimp from all sampling locations possess antibacterial activity against Vibrio spp., highlighting their potential as promising candidates for the development of biocontrol agents in sustainable aquaculture systems.
FARMING OF NILE TILAPIA (Oreochromis niloticus) AT THE KRIDO YUWONO FARM IN PANEMBANGAN Emyl Rohayati; Kasprijo
Journal Of Artha Biological Engineering Vol. 4 No. 1 (2026)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/6r5e8b07

Abstract

Tilapia farming is the practice of raising fish from fry to marketable size to meet increasing consumer demand. This study aims to evaluate tilapia farming activities at the Krido Yuwono Panembangan Fish Farming unit. The research methods employed were direct observation and active participation in all farming processes to obtain comprehensive and accurate data. The variables observed included farming stages, fish length and weight, survival rate (SR), specific growth rate (SGR), and feed conversion ratio (FCR). The farming activities consisted of several stages, including pond preparation (drying, liming, fertilization, and water filling), seed stocking, feeding management, and water quality control. The results showed that the survival rate reached 86.67%, indicating a relatively high level of fish survival during the culture period. The specific growth rate was recorded at 1.77% per day, while the feed conversion ratio was 1.35, reflecting efficient feed utilization. Overall, the applied farming practices were effective and supported optimal tilapia production.
The Growth response of patin fish (pangasius sp.) To nutrinex enerc supplementation in feed at various dose levelsE LEVELS Muhammad Gilang Ramadhan; Ren Fitriadi; Agnes Heratri; Maghfira Selia Irawati; Sreshi Asa Mahendra
Journal Of Artha Biological Engineering Vol. 4 No. 1 (2026)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/byske238

Abstract

Optimizing feed utilization is an important strategy in increasing the productivity of Patin Fish (Pangasius sp.) rearing. The addition of multivitamin supplements to feed can increase metabolic efficiency and nutrient utilization, thereby improving fish growth performance. This study aims to evaluate the effects of Nutrinex EnerC supplementation at different doses on the growth performance and feed utilization efficiency of Patin Fish. The study used a Completely Randomized Design (CRD) with three treatments and three replications, namely P1 (0 g/kg feed), P2 (3 g/kg feed), and P3 (6 g/kg feed). Fish were reared in a biofloc system and fed at a feeding rate of 3% of body weight during the maintenance period. The parameters observed included weight growth, length growth, Average Daily Growth (ADG), Specific Growth Rate (SGR), Feed Conversion Ratio (FCR), feed efficiency, and Survival Rate (SR). Data were analyzed using analysis of variance (ANOVA) followed by Tukey's test at a 95% confidence level. The results showed that Nutrinex EnerC supplementation significantly affected weight growth, ADG, SGR, FCR, and feed efficiency (p<0.05), but did not significantly affect length growth and fish survival. The best treatment was obtained at a dose of 6 g/kg feed which resulted in weight growth of 8.87 ± 0.40 g, ADG 0.25 ± 0.01 g/day, SGR 4.84 ± 0.02%/day, FCR 0.54 ± 0.02, and feed efficiency of 186.59 ± 8.50%. The results of this study indicate that Nutrinex EnerC supplementation has the potential to increase feed utilization efficiency and growth performance of Patin Fish in intensive rearing systems.
The Method Study And Stability Testing on Liquid Probiotic Products at CV. Pradipta Paramita, Karanganyar Regency, Central Java Alzahni Meila Putri; Ren Fitriadi; Agnes Heratri; Maghfira Selia Irawati; Kamilatun Nisa&#039; Agustina
Journal Of Artha Biological Engineering Vol. 4 No. 1 (2026)
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/t6rb6c18

Abstract

Evapond Bacillus is a liquid probiotic product developed to enhance the health of fish and shrimp. Maintaining its quality can be achieved through encapsulation using coating materials. This study applied an experimental method with three treatments and one replication. The encapsulation material concentration was set at 10% of the liquid volume, with variations in the ratio of maltodextrin and guar gum, namely (70%:30%), (50%:50%), and (60%:40%), each prepared in 100 ml of water. The experiment was conducted for two months to evaluate product stability after encapsulation. The results showed that the combination of maltodextrin and guar gum did not significantly affect the stability of Evapond Bacillus. However, the (60%:40%) ratio demonstrated relatively better performance compared to other treatments. These findings indicate that although the differences were not statistically significant, certain compositions may provide more favorable outcomes. Further studies are recommended using additional or alternative encapsulation materials to obtain more optimal and applicable results.

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