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Journal of Artha Biological Engineering
ISSN : 30464889     EISSN : 30324394     DOI : https://doi.org/10.62521/pm6jjq40
The scope of this journal is to publish original research papers, research notes, and reviews in the field of Biological Engineering widely. As such, high quality research papers or reviews dealing with any aspect of Biological Engineering are welcome. Papers that are theoretical, interpretive or experimental.
Articles 25 Documents
Effectiveness of Minaraya Probiotics on The Growth Rate and Immune Response of Catfish (Clarias gariepinus) fitriadi, ren; Sukardi, Purnama; Muhammad Riady, reza; nurhafid, mohmammad; Heratri, Agnes; Selia Irawati, Maghfira
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/bdxbgh67

Abstract

The growing demand for catfish in the market is prompting farmers to consistently enhance their production capacity. Several obstacles are encountered during production growth, such as pathogen attacks, suboptimal growth, and low survival rates. This study aimed to determine the effectiveness of Minaraya probiotics with different doses in feed on the growth and immune response of catfish. The method used in this study was an experimental method with a completely randomized design (CRD) consisting of four treatments: control (without probiotics), and treatments P1, P2, and P3 with the addition of Minaraya probiotics at 2, 5, and 10 mL per 2.5 kg of feed, respectively, each with three replications. The parameters observed included survival rate, SGR, DGR, FCR, PER, AF, RB, and leukocyte differentiation. The results showed that the survival rate in all treatments was relatively high, ranging from 95% to 98.89%. The results also showed that the addition of Minaraya probiotics significantly (p<0.05) increased growth parameters (SGR and DGR) and feed efficiency (FCR and PER), with the best dose being in treatment 3 (P3) at a dose of 10 mL/2.5 kg of feed. On the other hand, the non-specific immune response also increased in the treatment given Minaraya probiotics, as indicated by increased phagocytic activity, respiratory burst, and proportion of lymphocytes, neutrophils, and monocytes, as well as an increase in the total number of erythrocytes after Aeromonas hydrophila infection. The application of Minaraya probiotics in a sustainable cultivation system can improve catfish productivity and health.
Screening of Amylolytic Bacteria from the Intestines of Tilapia (Oreochromis niloticus) Cultivated in Rice Fish Farming in Panembangan Village, Cilongok District, Banyumas nabila, salsa; Kasprijo; Oktavia Kusuma, Rima; Marnani, Sri; Ikhwan Ihtifazhuddin, Muhammad
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/p5vczh22

Abstract

Amylolytic bacteria in the digestive tract of tilapia are bacteria that produceamylase enzymes, which function to break down carbohydrates, thereby aidingthe digestive process. The presence of amylolytic bacteria is influenced by feedand the environment. The purpose of this study was to determine the proportionand activity index of amylolytic bacteria in the intestines of tilapia. This studyused an observational method with purposive sampling. Amylolytic bacteriawere isolated from the intestines of tilapia in Rice fish farming Blocks A and E.The successfully isolated bacteria were grown on 1% starch agar medium withan incubation time of 48 hours and observed at 24-hour intervals. Then, severalcharacteristic tests were carried out, including Gram staining, KOH, catalase,and oxidase tests. Based on the results of the study, the proportion of amylolyticbacteria was found in 10 isolates with a proportion of 44% and 12 isolates witha proportion of 48% in the intestines of tilapia from the two blocks. The averageamylolytic activity index obtained from block A was 0.96 ± 0.237, and fromblock E was 1.49 ± 0.501. The largest amylolytic activity index was obtainedin isolate KSA 19 with a value of 1.33 from block A, while from block E, thelargest amylolytic activity index value was 2.6 in isolate KSE 5.
Fluctuation of Green Algae and Effects on the Growth of Pacific White Shrimp (Litopenaeus vannamei) Pinandita, Mia; Nugrayani, Dewi; Setyawan, Agung Cahyo; Marzuki, Imam
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/tcxv0s65

Abstract

Algae are eukaryotic organisms with chlorophyll. Green algae contain chlorophyll, allowing them to photosynthesize directly and provide shrimp ponds with food. This study is entitled Fluctuations of Green Algae and Effects on the Growth of Pacific White Shrimp (Litopenaeus vannamei) in CV. Cemarasewu Sumber Rejeki, Cilacap. This observational study Oct–Dec 2022 monitored a pond in Cilacap. Water green algae and Pacific White Shrimp samples were taken every seven days age 64–120 days. Algae density was calculated hemocytometer, while shrimp growth parameters MBW, ADG, FCR, SR and water quality temperature, pH, DO, salinity, nutrients were measured. Data were analyzed descriptively to observe the relationship between algae fluctuations and shrimp growth. The results of the study showed that green algae experienced fluctuations during the Pacific White Shrimp cultivation period. The presence of green algae can have a good effect on the growth rate of Pacific White Shrimp. The growth rate of Pacific White Shrimp in the research pond was classified as good. The results obtained by ADG in plot B13 0.39%, B14 0.45% and B15 0.38%. MBW in plot B13 30.75 g, B14 31.68 and B15 32.05 g. FCR in plot B13 1.42, B14 1.45 and B15 1.39 and SR in plot B13 66.7%, B14 71.5% and B15 70.7%.  
Monitoring Of Water Quality In Vanamei Shrimp (Litopenaeus vannamei) Farming In Cv. Cemarasewu Sumber Rejeki, Cilacap Pinandita, Mia; Nugrayani, Dewi; Marzuki, Imam
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/jma13a03

Abstract

Pacific White Shrimp (Litopenaeus vannamei) is a frequently cultivated shrimp species. This is because it offers promising prospects and profits. Superior vannamei can be produced by considering several aspects of the cultivation process. Internal factors include the origin and quality of the seeds, as well as external factors, including cultivation water quality, feeding, technology, and pest and disease control. This research was conducted from September 15 to October 15, 2022, at CV. Cemarasewu Sumber Rejeki, Jetis Village, Cilacap, Central Java. The purpose of this study was to determine the water quality conditions in Pacific White Shrimp rearing ponds at CV. Cemarasewu Sumber Rejeki, Cilacap. Three ponds were used for data collection: plots B13, B14, and B15, with shrimp rearing ages of 100-129 days. Research results indicated that plots B13, B14, and B15 at CV. Cemarasewu Sumber Rejeki provides good water quality for Pacific White Shrimp cultivation. The results of water quality parameters measured in this study include: brightness up to 35 cm, temperature ranging from 27-29 ºC, pH ranging from 7.6-8.3, salinity ranging from 14-22 ppt, alkalinity ranging from 125-169.6 ppm, TOM ranging from 70.4-79.2 ppm, DO ranging from 4.32-4.98 ppm, phosphate ranging from 0.25-0.5 ppm, nitrite ranging from 0.013-0.085 ppm, nitrate 0 ppm, ammonia ranging from 0.00-0.085 ppm, ammonium ranging from 0.00-0.50 ppm and plankton ranging from 2,500-282,500. It can be concluded that the water quality in pond plots B13, B14, and B15 at CV. Cemarasewu Sumber Rejeki meets the appropriate standards to support Pacific white shrimp cultivation.
Proteolytic Bacterial Activity from the Digestive Tract of Udikan Fish (Tor Sp.) Caught in Banjaran River Ruswanda, Roni; Ekasanti, Anandita; Kusuma, Rima Oktavia
Journal Of Artha Biological Engineering Vol. 3 No. 2 (2025): Volume 3 no 2 2025
Publisher : PT. Artha GenetikaLab Indonesia

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.62521/jmdy2152

Abstract

The type of feed commonly consumed by fish in nature can be predicted from the presence of microorganism communities in their digestive tract. One of the bacteria that plays an important role in the digestive tract is proteolytic bacteria. The purpose of this study was to determine the activity index and proportion of proteolytic bacteria from the digestive tract of udikan fish (Tor sp.) caught in the Banjaran River. The method used in this study was observation with a sampling technique conducted by purposive sampling. The activity index of proteolytic bacteria from the digestive tract of udikan fish (Tor sp.) was relatively low with an average value of 0.06 in 24-hour observations and 0.05 in 48-hour observations. The proportion of proteolytic bacteria obtained from the digestive tract of udikan fish (Tor sp.) was 33%.

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