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Jurnal Penelitian Saintek

saintek
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Published by Universitas Negeri Yogyakarta

ISSN : 14123991 EISSN : 25287036 DOI : 10.21831

Core Subject : Science,

Decision Sciences, Operations Research & Management

Arjuna Subject : -

Articles 286 Documents

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THE EFFECT OF ADDITIVE COMBINATION AND Azotobacter sp. TOWARDS Glycine max. L. GROWTH Yunia Vella Alfani; Pudjawati Suryatmana; Ade Setiawan
Jurnal Penelitian Saintek Vol 24, No 2: Oktober 2019
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

This study was aimed at determining the increasing of growth and production of soybean crops by giving Azotobacter sp. and additive materials such as coconut water, molasses, and bran. This research was conducted on Januari 2018 to April 2018 at Ciparanje Experimental Field Faculty of Agriculture. Universitas Padjadjaran, Jatinangor, Sumedang, West Java Province, on ± 774 meters above sea level (asl) using single factor randomized block design with 10 treatments; Azotobacter sp. and additive materials combination such as coconut water, molasses, and bran in three repetitions. The observations on the parameters observed in the final vegetative phase were on plant height, chlorophyll content, and population of Azotobacter sp. using Total Plate Count (TPC) method.The results show that the application of combination additives materials and Azotobacter sp. effect on populations Azotobacter sp., chlorophyll content, plant height and number of soybean pods (Glycine max L.). Application of coconut water independently, water added with Azotobacter sp., and combination of additive with Azotobacter sp. can give the best effect to the content of chlorophyll, plant height, and soybean crops components (Glycine max. L.).PENGARUH KOMBINASI BAHAN ADITIF DAN Azotobacter sp. TERHADAP PERTUMBUHAN Glycine max. L.Penelitian ini bertujuan untuk mengetahui pertumbuhan dan produksi tanaman kedelai dengan memberikan Azotobacter sp. dan aditif air kelapa, molase, serta dedak. Penelitian ini dilaksanakan pada Januari 2018-April 2018 di Kebun Percobaan Ciparanje Fakultas Pertanian Universitas Padjadjaran, Jawa Barat pada ± 774 meter di atas permukaan laut (dpl). Penelitian ini menggunakan rancangan acak kelompok dengan 10 perlakuan pemberian Azotobacter sp. dan aditif air kelapa, molase, serta dedak dengan tiga ulangan. Pengamatan terhadap parameter yang diamati pada fase vegetatif akhir yaitu tinggi tanaman, kandungan klorofil, dan populasi Azotobacter sp. dengan metode Total Plate Count (TPC). Hasil penelitian menunjukkan aplikasi kombinasi bahan aditif dan penambahan pupuk hayati Azotobacter sp. berpengaruh terhadap populasi Azotobacter sp., kadar klorofil, tinggi tanaman dan jumlah polong tanaman kedelai (Glycine max L.). Aplikasi air kelapa secara mandiri, air kelapa yang ditambahkan dengan Azotobacter sp., dan kombinasi bahan aditif dengan Azotobacter sp. dapat memberikan pengaruh terbaik terhadap kandungan klorofil, tinggi tanaman, dan komponen hasil tanaman kedelai (Glycine max. L.).

DESIGN OF WORK EQUIPMENT SYSTEM AND STEAM SYSTEM WITH HYDRAULIC SYSTEM Heri Wibowo
Jurnal Penelitian Saintek Vol 9, No 1: April 2004
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The weight vehicle was used to work the weight job of human such as digging loading and lifting. the materials It was completed bv system work such as attachment svstem and driving system. The both of system must he supported by the engine follow with hydraulicsystem. Design 0/ attachment svst m used cylinder hydraulicmovement for work 01 digging. loading or lifting. and so do cylinder hydraulic was used to turning all bodv of vehicles. The movement and power a/attachment system and driving system was designed to could work a/digging. loading and lifiing. The result calculation of attachment force, the /ifi hvdraulic cylinder is designed with 2cvlinders. each has 15 em cvlinder diameter and I em width 0/cylinder with uses stainless steel (0., = 5100 kg/em']. The dump cylinder is designed with I cvlinder. has /8 CI7I ofcylinder diameter and I em width of cvlinder with lise the same materials. Hydraulic svstem uses the hydraulic pump to central work of attachment system and driving system [hut drive from engine power.

DETERMINING ISOSBESTIC POINT AND DETERMINATION OF P-NITROFENOL COMPOUND by SPEKTROFOTOMETRI Heru Pratomo
Jurnal Penelitian Saintek Vol 8, No 1: April 2003
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The determined of isosbestic point from weak acids or weak bases areusually important for indicator solutions. The indicators that used in acid-base titration have pH exactly, and this is usefulfor determine the end of titration. The aims of this research are (1) to determine ofwavelength on isosbestic point of p-nitro phenol, and (2) to determine of dissociation constant of p-nitro phenol. The determined of isosbestic has done with spectrophotometric method, to the p-nitro phenol solutions in several pH, at region of wavelength between 280 nm - 450 nm. In this research, indicate that the isosbestic point ofp-nitro phenol at the wavelength of 348 nm, and pH about 6.7. From this research also can be determined that the dissociation constant (KaYof p-nitro phenol is 6.48 x 10-8,not too different compared with K, literature, is7.08 x 10-8.

COEFFICIENT VALUE OF NUTRITIONAL FISH AND DIVERSITY PLANKTON LAKE RAWA PENING JAWA TENGAH Sukiya Sukiya
Jurnal Penelitian Saintek Vol 8, No 1: April 2003
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The objective of the research is to investigate the nutrition vaLuecoefficient of fish and plankton diversity of Rawapening Lake due to pollutant from inlet water. Research was conducted in west, middle and south sides of the lake. The independent variable was the physicochemical quality of the water body, and the dependent one was the nutrition value coefficient of fish and plankton diversity. Samples were the mixture of 10 litres surface and 10 litres of two-meter deep' waterfor every station sampled. Temperature, clarity, total suspended solid and pH measured in situ. Microwinkler method was applied to measure alcalinity, DO and free CO2 in water body. Identification of plankton diversity used the book of Fresh Water Biology by Edmonson published in 1966 and Planktonology by Sachlan published in 1978, the nutrition value coefficient of fish was calculated using Fulton formula. Results show that 43 plankton and 16 necton species are investigated. Based on the coefficient of nutrition value it is concluded that Rawapening lake is consideredfeasiblefor nilafish (Oreochromys nilotica) to culture.

TEST ACTIVITIES SOME COMPOUNDS OLIGORESVERATROL RESULT OF INSULATION OF SKIN HOPEA ODORATA PLANT AS PREVENT 2-DEOKSIRIBOSE DEGRADATION Sri Atun
Jurnal Penelitian Saintek Vol 13, No 1: April 2008
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The objective of the study were measured inhibitory of 2-deoxyribose degradation activity of balanocarpol (1), ampelopsin H (2), hopheaphenol (3), and hemlesyanol C (4) which are resveratrol compounds from isolation of stem bark of Hopea odorata, and to study of different activity as 2-deoxyribose degradation activity from this compounds. Methods of this study to measured 2-deoxyribose degradation activity test with Fenton reaction method. Activity of 2-deoxyribose degradation activity calculated as percentage absorbansi decreased sample with bioactive compounds compared with a blank. As positif control used vitamin C and BHT (Butylated Hydroxy Toluena). The result of this study showed activity each compounds as 2-deoxyribose degradation activity of balanocarpol (1), ampelopsin H (2), hopheaphenol (3), and hemlesyanol C (4) with an IC50 1802,3; 4840,0; 61,8 and 425,5 mg/ml respectively. Hopeaphenol (3) showed more active than vitamin C (IC50 83,9 mg/ml) and BHT (1328,1mg/ml)

TWO-LINK FLEXIBLE MANIPULATOR AS AN ALTERNATIVE IN IMPROVING ENERGY EFFICIENCY OF MANIPULATOR ROBOT Moh Khairudin
Jurnal Penelitian Saintek Vol 20, No 2: Oktober 2015
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The dynamic model and characterization of a two-link ﬂ exible manipulator as an alternative for manipulator robot to achieve the efﬁ ciency on consuming electrical energy comparing with rigid manipulator. A planar two-link ﬂ exible manipulator was combined with structural damping, hub inertia and payload that moving horizontally. A dynamic model system was developed using the combination of Euler-Lagrange and assumed mode methods. Armed with the developed model, then some simulations were done to examine the dynamic model and the response of the system on the hub and the end point of both link that were presented and analyzed in function of time and frequency. The results show that the bang-bang input of 0.15 volt can move the link 1 on 55 0 and link-2 on 122 respectively. It means that the dynamic model uses more efﬁ cient energy compared with rigid manipulator robot which required bigger input for moving.

THE EFFECT OF COLEUS TUBEROSUS CONTAINING RESISTANT STARCH TYPE 3 CRACKERS ON HYPERCHOLESTEROLEMIA SUFFERING ANIMALS Mutiara Nugraheni; Siti Hamidah; Rizqie Auliana
Jurnal Penelitian Saintek Vol 21, No 1: April 2016
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

This study was aimed at determining the effect of Coleus tuberosus containing resistant starch type 3 on the hypercholesterolemia suffering animals. The research method used was lipid proﬁ le analysis on experimental animals suffering from hypercholesterolemia. The results showed that the consumption of Coleus tuberosus crackers containing resistant starch type 3 as a raw material for making crackers is able to decrease the lipid proﬁ le (total cholesterol, triglycerides, LDL) and increase the HDL level.

EXPLORATION OF THERMOFILIC BACTERIA POST ERUPSI MERAPI AS EXTRACELULAR ENZYME MANUFACTURER Anna Rakhmawati; Evy Yulianti
Jurnal Penelitian Saintek Vol 17, No 1: April 2012
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Penelitian mengenai bakteri termofilik pasca erupsi Merapi masih sangat terbatas padahal masih banyak potensi yang dapat diteliti, salah satunya mengenai enzim ekstraseluler yang dihasilkannya. Tujuan penelitian ini adalah untuk isolasi, karakterisasi dan memperoleh isolat bakteri termofilik yang mampu menghasilkan enzim ekstraseluler. Isolasi bakteri termofilik dilakukan dengan menggunakan 2 (dua) metode yaitu dilution dan enrichment dengan media Nutrient Broth kemudian diinkubasi pada 55 ºC, dilanjutkan dengan seleksi pada suhu 70 ºC. Karakterisasi yang dilakukan, yaitu karakterisasi morfologi koloni. Setelah itu dilakukan skrining aktivitas enzim amilase, protease, dan selulase. Hasil penelitian menunjukkan bakteri termofilik pasca erupsi Merapi yang diisolasi dari sampel air dan pasir Kali Gendol Atas dengan suhu inkubasi 55⁰C diperoleh 480 isolat, setelah diseleksi pada suhu 70 ⁰C diperoleh 253 isolat. Karakter fenotipik isolat bakteri termofilik pasca erupsi Merapi menunjukkan keanekaragaman morfologi koloni meliputi warna, bentuk, ukuran, tepi, dan elevasi koloni. Isolat bakteri termofilik yang menghasilkan enzim ekstraseluler amilase sebanyak 9 isolat, enzim protease sebanyak 4 isolat, dan 1 isolat penghasil enzim selulase pada suhu inkubasi 70 ⁰C .Kata kunci: bakteri, termofilik, enzim ekstraseluler

REDUCING SULPHATE AND NATURAL ZEOLITE IN Mn METAL SEDIMENTATION USING BACTERIA CONSORTIUM Nur'aini Purnamaningsih; Endah Retnaningrum; Wahyu Wilopo
Jurnal Penelitian Saintek Vol 22, No 1: April 2017
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

The objective of this study were determining the effects of natural zeolite from Wonosari on the activites of Sulfate Reducing Bacteria in Mangan (Mn) sedimentation of continous culture scale, and identifying the biofilm character of Sulfate Reducing Bacteria on consortium activites on natural zeolite using continous culture scale. The first studies was activation of zeolites, followed by testing the activity of consortium activities of Sulfate Reduction Bacteria in continuous culture scales; and biofilm characterization of Sulfate Reducing Bacteria consortium. The consortium of Sulfate Reducing Bacteria derived from goat feces. The size of zeo natural zeolites used in the consortium activity test of Sulfate Reducing Bacteria in Mangan sedimentation was 0.8 to 1.2 cm. The results showed that the consortium of Sulfate Reducing Bacteria in the bioreactor with the addition of zeolite had an activity that was more effective than the bioreactor without the addition of zeolite, where the pH was increased to pH 6.9; Mangan sedimentation was 61,16%, and the higher MPN index. Zeolite in a bioreactor with biofilm formation in 5th days and 9th days seems that there were biofilm consortium of Sulfate Reducing Bacteria growing attached to the surface of the zeolite.

CONTROL OF FLOWER PATHOGEN ACTIVITIES OF SOYBEAN PLANTS IN STRAIN COCONUTION PSEUDOMONAS SP. AND BACILLUS SP. WITH BRADYRHIZOBIUMJAPONICUM Nunung Sulistyani; Aris Tri Wahyudi; Giyanto Giyanto
Jurnal Penelitian Saintek Vol 18, No 1: April 2013
Publisher : Institute of Research and Community Services, Universitas Negeri Yogyakarta

Plant growth promoting rhizobacteria (PGPR) is a bacterial colonization of plant roots that can stimulate the growth of plants directly or indirectly. The PGPR mechanism indirectly results in plant growth as it suppresses the growth of phytopathogen. This study aims to determine the ability of Pseudomonas sp. and Bacillus sp. in controlling the pathogenic root fungal pathogenic fungi. Isolates in this study were Pseudomonas sp. Crb 3, Crb 17, Crb 17i, Crb 68 and Bacillus sp. Cr 24, Cr 44, Cr 66 co-inoculated with B. japonicum in soybean plants in greenhouses. An antagonistic activity test was performed on all isolates and all isolates showed no antagonistic activity. Treatment of combination of strain Pseudomonas sp.Crb 17, Crb 17i and Bacillus sp. Cr 24 inoculated with B. japonicum suppresses the growth of root rot fungi and increases peroxidase. Therefore, Pseudomonas sp. Crb 17, Crb 17i and Bacillus sp. Cr 24 can be used as a biocontrol agent for pathogenic fungi causing plant root disease.

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