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Jurnal Akademika Biologi
Published by Universitas Diponegoro
ISSN : -     EISSN : 26219824     DOI : -
Core Subject : Science,
Biochemistry, Genetics & Molecular Biology
Jurnal Akademika Biologi diterbitkan oleh Departemen Biologi Fakultas Sains dan Matematika Universitas Diponegoro Semarang. Jurnal ini sebagai media publikasi hasil karya ilmiah lulusan S1 Departemen Biologi. Jurnal Akademika Biologi menerima artikel-artikel yang berhubungan dengan bidang ilmu biologi.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 234 Documents
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DETEKSI MRSA (Methicillin Resistant Staphylococcus aureus) PADA PASIEN RUMAH SAKIT DENGAN METODE MALDI-TOF MS DAN MULTIPLEX PCR Dian Wahyu Kemalaputri; Siti Nur Jannah; Anto Budiharjo
Jurnal Akademika Biologi Vol. 6 No. 4 Oktober 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Methicillin Resistant Staplylococcus aureus (MRSA) is a strain of Staphylococcus aureus which has been resistant to methicillin antibiotics and β-lactam group. The mechanism of MRSA resistance occurs because the Staphylococcus aureus produces Penicillin Binding Protein (PBP2a) encoded by the mecA gene that has low affinity to methicillin. Nowadays, MRSA is tested by resistance test using Oxacillin 1 ug. This method requires pure isolate and bacterial culture, so that the results can be seen in 5 days. To find a rapid and precise diagnostic technique for detecting MRSA, mecA gene detection utilizing PCR multiplex technique as the alternative diagnostics. This study aims to find a quick and precise alternative diagnostic technique for MRSA examination, which is utilizing MALDI-TOF MS and multiplex PCR technique.Keywords: MRSA, Staplylococcus aureus, MALDI-TOF MS, Multiplex PCR.
POTENSI RIZOBAKTERI PEMBENTUK ENDOSPORA DARI TANAMAN PADI SEBAGAI BIOKONTROL FITOPATOGEN Xanthomonas oryzae Maerani Sumarno; Anto Budiharjo; Sri pujiyanto
Jurnal Akademika Biologi Vol. 3 No. 3 Juli 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Xanthomonas oryzae is phytopathogen causing bacterial leaf blight which decreases in agricultural product reaching 20-70 % in Asia. Bacterial leaf blight symptoms is characterized by the formation of lines in the leaf blade turnings yellow, then white, causing the plant to wither and die. Endospore-forming rhizobacteria are soil microbes potential as biocontrol to inhibit phytopathogen growth. The aims of this study were to isolate endospore-forming rhizobacteria from rice plant and determine its ability as biocontrol against X. oryzae. The methods used consisted of isolation, antibacterial activity test, molecular identification, and biochemical characterization. Twenty isolates of endospore-forming rhizobacteria were obtained from the isolation of the rice crop. Isolate P-10 had the greatest ability against X. oryzae with inhibition zone of 18.89 mm. Molecular identification using 16S rRNA gene showed that isolates P-10 had 98 % homology with Bacillus pumilus. Biochemical characterization showed the isolate P-10 had a rod-shaped with center of endospores, gram-positive, catalase positive, are motile, negative in starch hydrolyze, not forming gas on glucose, these characteristics fitted with B. pumilus character. Keywords : Rice plant, antibacterial, Xanthomonas oryzae, endospore-forming, rhizobacteria
Keanekaragaman Vegetasi Riparian Sungai Panjang Bagian Hilir di Kecamatan Ambarawa Kabupaten Semarang Famella Beatris Putri Nurika; Erry Wiryani; Jumari Jumari
Jurnal Akademika Biologi Vol. 8 No. 1 Januari 2019
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Sungai Panjang merupakan salah satu sungai yang berumuara ke Danau Rawa Pening. Salah satu wilayah yang dilalui Sungai Panjang bagian hilir adalah Desa Bejalen di Kecamatan Ambarawa kabupaten Semarang. Sejauh ini belum banyak didapatkan informasi mengenai komposisi vegetasi riparian di kawasan tersebut.Tujuan penelitian ini adalah mengkaji keanekaragaman vegetasi riparian yang ada di Sungai Panjang bagian hilir. Penelitian dilakukan pada bulan Juni-Juli 2018 di Kecamatan Ambarawa Kabupaten Semarang dan Laboratorium Ekologi dan Biosistematik Departemen Biologi Undip. Metode yang digunakan dalam penelitian ini adalah metode plot dengan jumlah plot sebanyak18 dan terbagi dalam 3 stasiun penelitian. Ukuran plot 10 m x 10 m untuk pohon, sub plot 5 m x 5m untuk perdu dan subplot 1 m x 1 m untuk herba. Hasil penelitian didapatkan 11 spesies tanaman pohon, 15 spesies tanaman perdu dan 23 spesies tanaman herba. 
PENGARUH CARA PEMUPUKAN PUPUK CAIR NANOSILIKA MELALUI MEDIUM & PENYEMPROTAN PADA PERTUMBUHAN SUBKULTUR BIBIT ANGGREK Dyah Ayu Kusuma Ningrum; Erma Prihastanti; Endah Dwi Hastuti; Agus Subagyo
Jurnal Akademika Biologi Vol. 5 No. 2 April 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

One way to increase supply orchid seedlings by subculture. Subculture of orchids often have hyperhidrisition. Hyperhidrisition can be overcome by unsure silica. This study aims to determine the effect of different ways through spraying fertilizer with the addition of fertilizer in the medium and analyze the results of the most effective influence on the growth of subculture of orchids. The study was conducted using a completely randomized design (CRD) with a single factor nanosilica fertilizer treatment in the medium and through spraying and each treatment was repeated three times. The treatment in this study are as follows: liquid fertilizer nanosilica M0K0 = 0% in the medium, liquid fertilizer nanosilica M0K1 = 75% in the medium, liquid fertilizer nanosilica M1K0 = 0% through spraying and liquid fertilizer nanosilica M1K1 = 75% by spraying. The data obtained were analyzed by ANOVA significance level of 95%. The results showed nanosilica fertilizer through spraying and the medium Vacint and Went (VW) increased the growth of subcultures Dendrobium sp. Liquid fertilizer application nanosilica with a concentration of 75% through the subculture medium most effectively promote the growth of plantlets subculture Dendrobium sp. Keywords: Dendrobium sp., subculture, fertilization, spraying, nanosilica
KOMUNITAS RHIZOBAKTERIA TANAMAN TEH DENGAN APLIKASI FORMULA BIOIMUNIZER (Chryseobacterium Sp DAN Bacillus Sp) BERDASARKAN GEN 16S rRNA Agnistisya Widaranti; Siti Nur Jannah; Hermin Pancasakti Kusumaningrum; Dwi Ningsih Susilowati
Jurnal Akademika Biologi Vol. 5 No. 4 Oktober 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Commodity of tea (Camellia sinensis) has an important role in the national economy, especially in the field of agro-industries. Based on data obtained from the Directorate General of Plantation, the tea plant productivity declined over the last few years. This is due to the pest attack which result in decreasing the productivity of the tea plant. Increased crop productivity of tea have been done, such as the use of herbicides and insecticides, but until now there is very little effort to increase the production of tea plants by the use of biological agents. Chryseobacterium sp and Bacillus sp are rhizobacteria in tea rhizosphere that could potentially be used as a biocontrol agent (bioimmunizer). The purpose of this study is to determine the community rhizobacteria in tea soil with the addition of bioimmunizer based on 16S rRNA gene using T-RFLP technique. The method used in this research is T-RFLP technique (Terminal Restriction Fragment Length Polymorphism) using enzymes Msp I and Rsa I. The calculated value are relative abundance, Shannon diversity index (H '), evenness index (E), and dominance index. The results of this study indicate that soil samples with the addition of bioimunizer consisting of Arthrobacter sp, Bacillus sp, Actinobacteria, and Chryseobacterium sp.Keyword: T-RFLP, Chryseobacterium sp, Bacillus sp
Pengaruh Jenis dan Konsentrasi Larutan Perendam Alami Terhadap Perkecambahan Biji dan Pertumbuhan Bibit Kelapa Sawit (Elaeis guineensis Jacq). A Amirudin; Endah Dwi Hastuti; Erma Prihastanti
Jurnal Akademika Biologi Vol. 4 No. 1 Januari 2015
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Oil palm (Elaeis guineensis Jacq) is one of the leading commodity Indonesian plantation product that has economic value International market. In the process of oil palm cultivation experience problems due to oil palm seeds have a hard endocarp and contain lignin content is high enough that it is difficult to germination. Purpose of this study the effect of the type and marinade solution concentration as well as the interaction between the type and concentration of  the  solution  marinade  for  seed  germination  and  seedling  growth  of  oil  palm  (Elaeis guineensis Jacq). This research is random full factorial design consisting of two factor 2 x 5. The first factor is the type of marinade: coconut water and cow urine, the second factor is the concentration of  the solution marinade. This study uses 10 treatments with 3 repetitions. Parameters observed that the percentage of germination, plant height, leaf number, leaf color, number of roots, fresh weight and dry  weight.  Analysis of the data used is Analysist of Variances (ANOVA) showed a significant effect if it continued with Duncan’ Multiple Range Test (DMRT) at the level of 95%. The results showed that the type of marinade effect on plantheight. The results showed that the percentage of seed germination is influenced by the concentration of soaking solution with the highest rates at a concentration of 75% was kind of marinade between coconut water and cow urine had no effect. Oil palm seedling growth is influenced by the type of solution shredded  coconut milk and cow urine concentration and interact with the marinade. Type shredded cow urine at a concentration of 75% to produce the highest seedling growth.Keywords: Oil Palm (Elaeis guineensis Jacq), Coconut milk, Cow urine,     Germination, Seed
KONSTRUKSI PLASMID PRHA SEBAGAI PEMBAWA GEN ARAA PENYANDI ENZIM L-ARABINOSA ISOMERASE DARI Thermotoga thermarum M Masfuroh; Anto Budiharjo; Hermin Pancasakti Kusumaningrum
Jurnal Akademika Biologi Vol. 6 No. 3 Juli 2017
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

D-tagatosa as natural low-calorie sweeterners is likely to be a sugar substitute for diabetics type II. D-tagatosa sweetness levels is by 92% the sweetness of sucrose, but only 38% of calories of sucrose. This study aimed to obtain a recombinant plasmid construction pRHA::araA. Results subcloning was then used in biotransformation processes produce D-tagatosa. Propagation vector soure pRHA   was done with the cloning procedure in E. coli TOP’10. The process  used the vector source cut with enzyme NcoI and XhoI and producing pRHA vectore for gene insertion araA. AraA gene amplification was done using Polymerase Chain Reaction (PCR)  with  primers of AITth-For and AITth-Rev. Ligation was done using T4 ligase enzyme and transformed in E. coli TOP’10 by heat-shock methods. E. coli was grown in LB medium Agar with ampicillin concentration of 100 mg/ml. Selection was made on a liquid LB and LB Agar with ampicillin concentration range of 100-200 mg/ml. Based on the result of electrophoresis visualization of the pRHA::araA recombinant plasmid isolation were negative. Keyword:  L-arabinosa isomerase, araA, D-tagatosa, T. thermarum, E. coli
Pengaruh Pemberian Kombinasi Pupuk Organik Padat dan Organik Cair Terhadap Porositas Tanah dan Pertumbuhan Tanaman Bayam (Amarantus tricolor L.) Imelda Anastasia; Munifatul Izzati; Sri Widodo Agung Suedy
Jurnal Akademika Biologi Vol. 3 No. 2 April 2014
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

The porosity of soil is one of the parameters of soil porosity. Tanah Merah in Madura island  has low fertility. This soil not good to used because the physical structure and  has low nutrition. The addition of organic materials from solid and liqiud organic fertilizer can increase soil porosity and spinach  growth. The research aimed to determine the effect of solid, liquid organic fertilizer, and their combination to  increase porosity and spinach growth. The design of the research is completely randomized design (RAL) with a single factor with combination treatment media, control treatment with no treatment to media (P0), media were given solid organic fertilizer (P1),  media were given liquid organic fertilizer (P2), and media were given combination of solid and liquid organic fertilizer (P3). Analysis of the data used  Analysis of Variance ( ANOVA ) continued by DMRT test with significance level of 95%. The results of the research showed organic fertilizer given effect to the increase in porosity of the soil and plant growth, as well as the provision of combination solid and liquid organic fertilizer are the most optimal fertilizer for soil porosity and spinach growth. Keywords : Spinach, solid organic fertilizer, liquid organic fertilizer, soil porosity, spinach growth
Kemampuan Akumulasi Logam Berat Tembaga (Cu) pada Akar Mangrove Jenis Avicennia marina (Forsk.) Dan Rhizophora mucronata (Lamk.) di Lahan Tambak Putri Kusumaning Dewi; Endah Dwi Hastuti; Rini Budihastuti
Jurnal Akademika Biologi Vol. 7 No. 4 Oktober 2018
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Aktivitas manusia dalam memanfaatkan pesisir seperti adanya pemukiman, industri serta pertanian sering menghasilkan bahan pencemar dapat membahayakan kehidupan perairan laut tersebut. Salah satu bahan pencemar yang dapat membuat lingkungan tercemar adalah logam tembaga (Cu). Keberadaan mangrove dikawasan perairan pesisir menjadi sangat penting karena vegetasi mangrove mempunyai kemampuan mengakumulasi logam berat. Penelitian ini bertujuan untuk mengetahui kemampuan akumulasi logam berat Cu pada akar mangrove jenis Avicennia marina dan Rhizophora mucronata. Rancangan penelitian ini menggunakan Rancangan Acak Lengkap 2 x 1 dengan 6 kali ulangan. Faktor I jenis (Avicennia marina dan Rhizophora mucronata), Faktor II organ (akar). Parameter penelitian ini yaitu kadar logam berat Cu pada sedimen, air, akar mangrove jenis Avicennia marina dan Rhizophora mucronata serta nilai BCF (Bio Concentration Factor). Hasil penelitian ini menunjukkan kadar logam berat Cu pada sedimen berkisar antara 0,685-1,545 mg/kg dan kadar logam berat Cu pada air berkisar antara 0,0035-0,4765 mg/l. The accumulation of Cu metal at the mangrove root of Avicennia marina is higher. 
STRUKTUR KOMPOSISI TUMBUHAN BAWAH TEGAKAN JATI DI KEBUN BENIH KLON (KBK) PADANGAN BOJONEGORO Nazilatun Nikmah; J Jumari; Erry Wiryani
Jurnal Akademika Biologi Vol. 5 No. 1 Januari 2016
Publisher : Departemen Biologi, Fakultas Sains dan Matematika Undip

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Abstract

Undergrowth vegetation are bottom layer of the tree community. Community of undergrowth vegetation are always identical as the weeds that had been considered as a disturber and harm. When viewed from another perspective undergrowth is a component of diversity. This study aimed to analyze the structure of the composition and reviewing the use of plants from three different plots under cropping Tectona grandis in seeds clones garden (KBK) Padangan. Data were collected at three research stations are in plots 1983, 1988 and 1993 using the plot as much as 36 squared, each size of 1 m x 1 m. Undergrowth plants were  obtained from three research stations is 19 species from 12 families. Family Fabaceae or legumes are most often found. The highest undergrowth had Importance Value Index (IVI) from plots 1983 and 1993 was Sentrosema (Centrosema pubescens) and on plots 1988 is grass (Brachiaria decumbens). Index of species diversity (H') in the three different  research stations showed the diversity of species are moderate at the region. Values of similarity index (IS) undergrowth vegetation showed  the plant community from  three research stations are different.Keywords: Undergrowth vegetation, species diversity, KBK  Padangan,  vegetation Analysis

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