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Biodiversitas Journal of Biological Diversity
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Articles 6,269 Documents
Identification of bacteria in Rhizophora mucronata leaf litter and sediment YULMA YULMA; ASIHING KUSTANTI; SOEMARNO SOEMARNO; MOHAMMAD MAHMUDI; BURHANUDDIN IHSAN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 6 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260648

Abstract

Abstract. Yulma, Kustanti A, Soemarno, Mahmudi M, Ihsan B. 2025. Identification of bacteria in Rhizophora mucronata leaf litter and sediment. Biodiversitas 26: 3054-3060. The mangrove ecosystem is an area that has high productivity, with litter production that contributes to increasing nutrients. This study aimed to determine the bacterial community in mangrove vegetation, especially in sediment and decomposed Rhizophora mucronata leaf litter. This study was conducted in the Mangrove and Crab Conservation Area. R. mucronata leaf litter was collected with a 2 × 2 m container. Furthermore, the leaf litter was dried at 105°C until its weight was constant. The decomposition rate and bacterial identification were measured at intervals of 14, 28, 42 and 56 days. Meanwhile, sediment was taken at different depths, namely 10, 20, and 30 cm, and as much as 250 g for each depth using a biopore drill. Identification of bacteria in litter and sediment was carried out with three tests, namely: Gram staining test (Gram-negative, Gram-positive and bacterial forms), primary test (3% KOH, 3% H2O2 and Oxidase) and further test (O/f, Glucose and motility). The results showed that the bacterial community that plays a role in decomposing organic matter and accelerating the rate of decomposition in R. mucronata leaf litter is diverse with different characteristics, including Bacillus sp., Aeromonas sp., Pseudomonas sp., Listeria sp., Actinobacillus sp., Micrococcus sp., and Acinetobacter sp. At the same time, the bacterial community found in the sediment includes Pseudomonas sp., Aeromonas sp., Micrococcus sp., Bacillus sp., Flavobacterium sp., Enterobacteria sp., and Listeria sp. The most dominant bacteria found is Bacillus sp. because one of the endophytic bacteria found in plant tissue can increase plant growth through mechanisms such as biological nitrogen fixation, phosphate and potassium solubilization.
Isolation and characterization of lactic acid bacteria from fermented quinoa (Chenopodium quinoa) for use in vegetable biopreservation SUNGEY NAYNEE SANCHEZ LLAGUNO; JEAN PIERRE MUÑOZ GUZMÁN; JUAN ALEJANDRO NEIRA MOSQUERA; JHONNATAN PLACIDO ALDAS MOREJON; KAROL YANNELA REVILLA ESCOBAR
Biodiversitas Journal of Biological Diversity Vol. 26 No. 7 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260708

Abstract

Abstract. Llaguno-sungey NS, Muñoz Guzmán JP, Neira Mosquera JA, Aldas Morejon JP, Revilla Escobar KY. 2025. Isolation and characterization of lactic acid bacteria from fermented quinoa (Chenopodium quinoa) for use in vegetable biopreservation. Biodiversitas 26: 3174-3182. The objective of this study was to isolate and characterize Lactic Acid Bacteria (LAB) from fermented quinoa for biopreservation of broccoli (Brassica oleracea var. italica) and zucchini (Cucurbita pepo). A completely randomized design with bifactorial arrangement A×B was used, where factor A corresponded to the type of vegetable and factor B to the LAB concentration (1.0 × 10? and 2.0 × 10? CFU/mL). Analysis of the fermented musts showed a decrease in pH to 3.60 in white quinoa and 3.83 in red quinoa at 72 hours. Soluble solids also decreased to 8.2 and 8.6 °Brix, respectively. As for acidity, it was significantly higher in red quinoa (0.21%) compared to white quinoa (0.18%) at the end of fermentation. Lactococcus lactis subsp. lactis was identified as the predominant LAB (bootstrap of 92%). In the biopreservation application, broccoli treated with 2.0 × 10? CFU/mL showed a decrease in pH from 5.20 to 5.11, an increase in acidity from 0.19 to 0.23%, and soluble solids from 4.43 to 4.87 °Brix. For zucchini, pH decreased from 6.31 to 6.14, and acidity increased from 0.12 to 0.15%. Weight loss was greater in zucchini (from 0.42 to 0.82%) than in broccoli (from 0.31 to 1.46%). Microbiological analysis showed a reduction in mold and yeast counts in broccoli treated with 2.0 × 10³ CFU/mL at day 10. In terms of sensory analysis, zucchini received better ratings. In conclusion, fermented quinoa musts represent an effective source of LAB with biotechnological potential for vegetable preservation, highlighting their positive effect on physicochemical, microbiological, and sensory parameters, as well as their viability as a natural alternative for postharvest preservation.
Present and future habitat suitability of an endemic tree frog, Zhangixalus jarujini, under climate change scenario CHANTIP CHUAYNKERN; NUTTAPONG KHAJITMATHEE; RATCHATA PHOCHAYAVANICH; WASSANA MAIPHROM; EKACHAI PHETCHARAT; AINGORN CHAIYES; PRATEEP DUENGKAE; YODCHAIY CHUAYNKERN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 9 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260917

Abstract

Abstract. Chuaynkern C, Khajitmathee N, Phochayavanich R, Maiphrom W, Phetcharat E, Chaiyes A, Duengkae P, Chuaynkern Y. 2025. Present and future habitat suitability of an endemic tree frog, Zhangixalus jarujini, under climate change scenario. Biodiversitas 26: 4445-4456. Zhangixalus jarujini, a tree frog in the family Rhacophoridae and endemic to northeastern Thailand, is increasingly threatened by habitat degradation and the impacts of climate change. This study utilized ecological niche modeling with the Maximum Entropy (MaxEnt) algorithm to evaluate the species’ current and future habitat suitability across Thailand, Laos, and Cambodia. A total of 16 confirmed occurrence records and 14 environmental variables were used to generate highly accurate models (mean AUC: 0.991±0.001). The most influential predictor variables were distance to water bodies (25.7%), precipitation seasonality (Bio15: 24.5%), precipitation of the warmest quarter (Bio18: 15.0%), normalized difference water index (NDWI: 13.3%), precipitation of the driest month (Bio14: 10.8%), and isothermality (Bio3: 9.2%). Under current conditions, the total area of suitable habitat was estimated at 9,964.58 km², comprising 5,837.82 km² of low suitability, 3,393.10 km² of medium suitability, and 733.67 km² of high suitability. Future projections, based on the ACCESS-CM2 climate model, were conducted under two Shared Socioeconomic Pathways: SSP2-4.5 and SSP5-8.5. By 2050, suitable habitat is projected to expand to 12,178.84 km² (22.22%) under SSP2-4.5 and 12,451.58 km² (24.96%) under SSP5-8.5. By 2070, it may reach 12,113.35 km² (21.56%) and 12,559.14 km² (26.44%) under the respective scenarios. High suitability areas are predicted to increase significantly, with a maximum of 1,228.58 km² under SSP5-8.5 by 2070, representing a 67.46% gain. The most influential environmental variables shaping habitat suitability were distance to water bodies, precipitation seasonality, and precipitation during the driest month. While these projections indicate a potential expansion of suitable habitat, actual population persistence may be constrained by ecological limitations, habitat fragmentation, and human pressures. Based on the model outputs and the availability of similar habitats in adjacent regions, this study strongly recommends systematic and intensive field surveys in Thailand, southern Laos, and northern Cambodia to identify potential undocumented populations and refine conservation priorities for Z. jarujini.
Seagrass ecosystems as habitats for target fish (Siganidae and Lethrinidae) in the waters of the Aruri Islands, Papua Province, Indonesia SELVI TEBAIY; JEMMY MANAN; EMANNUEL MANANGKALANGI; SAMPARI S. SURUAN; DENNY C. MAMPIOPER; PHILIPUS MUSYERI; AGNESTESYA MANUPUTTY; DODI SAWAKI
Biodiversitas Journal of Biological Diversity Vol. 26 No. 11 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261131

Abstract

Abstract. Tebaiy S, Manan J, Manangkalangi E, Suruan SS, Mampioper DC, Musyeri P, Manuputty A, Sawaki D. 2025. Seagrass ecosystems as habitats for target fish (Siganidae and Lethrinidae) in the waters of the Aruri Islands, Papua Province, Indonesia. Biodiversitas 26: 5726-5736. This study aimed to identify seagrass species, analyze their composition, percentage, and average cover, and determine the distribution and composition of fish species within seagrass ecosystems. Additionally, the relationships among various species and the presence of target fish, namely Siganidae and Lethrinidae, were examined. The research was conducted from November to December 2022 at three locations: Rayori Village, Nyambarai Village, and Munsaki Island in the Aruri Islands, Supiori District, Papua Province, Indonesia. Seagrass sampling employed the quadrat transect method modified from the Seagrass Watch protocol, while fish samples were collected using gill nets with the circle method. The results revealed the presence of six seagrass species in the study area, namely Cymodocea rotundata, Enhalus acoroides, Halodule pinifolia, Halophila ovalis, Oceana serrulata, and Thalassia hemprichii. Target fish, Siganus sp. and Lethrinus sp. were found at all observation sites, with 139 and 9 individuals, respectively. Furthermore, T. hemprichii showed a clear relationship with the presence of Siganus sp. and Lethrinus sp. indicating its essential role in seagrass ecosystems as a habitat for these target fish species. This study's findings underscore the crucial role of T. hemprichii in seagrass ecosystems and its implications for the conservation of these vital habitats in the Supiori District.
Decoding halophilic biofilm development across salinity gradients in hypersaline environments ILHAM MISBAKUDIN AL ZAMZAMI; DEFRI YONA; ABD RAHEM FAQIH; ANDI KURNIAWAN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 6 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d260622

Abstract

Abstract. Al Zamzami IM, Yona D, Faqih AR, Kurniawan A. 2025. Decoding halophilic biofilm development across salinity gradients in hypersaline environments. Biodiversitas 26: 2773-2785. Halophilic microorganisms possess distinctive adaptations that enable survival and biofilm formation under extreme salinity, exhibiting considerable promise for biotechnological applications. However, halophilic biofilms' structural and biochemical properties across varying salinity gradients remain insufficiently characterized. The present study investigates the biofilm formation on High-Density Polyethylene (HDPE) substrates in NaCl concentrations ranging from 2% to 40% over 21 days. A multidisciplinary approach was employed to characterize biofilm development and microbial responses. This approach included metagenomics, Confocal Laser Scanning Microscopy (CLSM), Fourier-Transform Infrared Spectroscopy (FTIR), and water quality analyses. The results demonstrated that optimal biofilm formation occurred at 20% NaCl, exhibiting peak microbial abundance, biovolume, protein content, and metabolic activity, accompanied by increased acid production, decreased pH, and reduced dissolved oxygen. Conversely, 40% NaCl elicited a substantial response characterized by severe osmotic stress, a marked deterioration in cell viability and biofilm integrity, and a notable elevation in turbidity consequent to cellular damage. Biofilms at 2% NaCl were stable but exhibited reduced metabolic activity and simpler structures. These results highlight the adaptive capacity of halophilic biofilms, especially at moderate salinities, providing essential insights into their ecological roles and applications in saline wastewater treatment and sustainable environmental management.
Tolerance of rhizospheric and endophytic microbes to pesticide residues and their potential for rice growth ERYNA ELFASARI RANGKUTI; ALINA AKHDIYA; ABDUL MUNIF; ISKANDAR ZULKARNAEN SIREGAR; SYAIFUL ANWAR
Biodiversitas Journal of Biological Diversity Vol. 26 No. 10 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261040

Abstract

Abstract. Rangkuti EE, Akhdiya A, Munif A, Siregar IZ, Anwar S. 2025. Tolerance of rhizospheric and endophytic microbes to pesticide residues and their potential for rice growth. Biodiversitas 26: 5267-5276. Rhizospheric and endophytic bacteria are recognized for their ability to enhance plant growth by producing hormones and acting as biocontrol agents. Therefore, this study aimed to identify potential rhizosphere and endophytic bacteria tolerant to three pesticide-active substances. Based on the antagonistic activity against Rhizoctonia solani, Bacillus sp. and Bacillus siamensis isolates achieved the highest inhibitory activities of 81.13 % and 78.55 %, respectively. Bacillus sp., B. siamensis, Acinetobacter radioresistens, and Providencia vermicola were able to solubilize phosphate. Production of Indole-3-Acetic Acid (IAA) was confirmed in Bacillus sp., Bacillus toyonensis, and Acinetobacter radioresistens, and all isolates also possessed the ability to fix nitrogen (N). Based on these parameters, Bacillus sp. and B. siamensis were selected for further analysis. The results showed that Bacillus sp. isolates supported rice seed tolerance to herbicides and insecticides in the range of 5-10 ppm and improved growth in the presence of difenoconazole and glyphosate at 10 ppm. Meanwhile, B. siamensis was tolerant to fungicides in the 5-10 ppm range. None of the selected isolates showed reduced growth after 24 h of incubation in the tolerance test for pesticide contamination. Observably, Bacillus sp. isolate treated with fipronil (5 ppm) was better than the control. It also matched the performance of the control treated with difenoconazole at 5 ppm. These suggested that the selected isolates improved rice growth and tolerance to the active ingredients of the pesticides, contributing to sustainable cultivation practices.
Morphophysiological evaluation of hybrid maize lines from peak and test crosses for high-yield development NASARUDDIN NASARUDDIN; MUH. FARID; MUHAMMAD FUAD ANSHORI; AMIN NUR; NENI IRIANY; ANNASTYA NUR FADHILAH
Biodiversitas Journal of Biological Diversity Vol. 26 No. 10 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261034

Abstract

Abstract. Nasaruddin, Farid M, Anshori MF, Nur A, Iriany N, Fadhilah AN. 2025. Morphophysiological evaluation of hybrid maize lines from peak and test crosses for high-yield development. Biodiversitas 26: 5204-5210. The increasing demand for maize in Indonesia amid stagnant productivity underscores the need for innovative breeding approaches to enhance yield potential and resilience. This study integrates morphophysiological trait analysis with hybrid maize lines derived from transgressive segregants and elite line, aiming to identify promising genotypes and key traits associated with high productivity under tropical conditions. A total of 96 genotypes, consisting of 60 hybrids, 20 parental lines and 4 commercial controls, were evaluated using an augmented design. Observations were made on agronomic and physiological traits. This is the first report combining peak and test cross schemes with morphophysiological evaluation in tropical maize, which broadens the genetic base and addresses the limitation of narrow parental pools. Several hybrid maize lines, particularly G29 (8.7 t.ha-1), G28, and G39, outperformed their parents in yield performance. Traits such as cob weight, number of seeds per row, and plant height exhibited high heritability and strong direct effects on yield, highlighting their suitability as selection criteria in breeding programs. Overall, integrating source traits (e.g., plant height, chlorophyll index) and sink traits (e.g., cob characteristics) provides an effective strategy for developing high-yielding and resilient maize hybrids suitable for smallholder-based production systems in tropical environments.
Forage species diversity and composition, and carrying capacity of rangelands in Teluk Wondama Regency, West Papua, Indonesia MARLYN N. LEKITOO; TEDDY LEKITOO; BUDI SANTOSO; EVI W. SARAGIH; ONESIMUS YOKU; BAMBANG TJ. HARIADI; DENY A. IYAI; LUKAS Y. SONBAIT
Biodiversitas Journal of Biological Diversity Vol. 27 No. 1 (2026)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d270111

Abstract

Abstract. Lekitoo MN, Lekitoo T, Santoso B, Saragih EW, Yoku O, Hariadi BT, Iyai DA, Sonbait LY. 2026. Forage species diversity and composition, and carrying capacity of rangelands in Teluk Wondama Regency, West Papua, Indonesia. Biodiversitas 27 (1): d270111. https://doi.org/10.13057/biodiv/d270111. Rangelands and natural pastures serve as the backbone of livestock feed. However, there is limited empirical data on their productivity and capacity to sustain livestock populations in lesser-known regions, such as Papua. The study aims to assess the botanical diversity and composition of forage in natural rangeland in Teluk Wondama Regency, West Papua, Indonesia, and to evaluate its carrying capacity. Botanical sampling was conducted by establishing 30 sample plots measuring 1×1 m2 in areas of 64 ha within the Warayaro natural rangelands. Forage productivity was estimated based on botanical composition, fresh forage, dry matter, and total digestible nutrients. The findings reveal that the rangeland in Warayaro is a heterogeneous pasture comprising 5 types of fodder, including 4 grasses and 1 legume species. Non-native species from 3 families dominated the pasture, namely Paspalum dilatatum, followed by Paspalum conjugatum, Centrosema pubescens, Eleusine indica, and Cyperus rotundus. The botanical composition consisted of 86.7% grasses and 13.3% legumes. Average forage yield is 2.0 t FM, 0.4 t ha-1 and 1.02 t ha-1 TDN. The existing Warayaro forage has a carrying capacity for cattle of 0.4 AU ha-1 yr-1 (FM), 0.3 AU ha-1 yr-1 (DM) and 0.8 AU ha-1 yr-1 (TDN). The Warayaro natural pasture has low production potential (264.7 kg TDN per 100 kg of livestock body weight) and moderate nutritional quality, with a protein content of only 11.2%. The Warayaro pasture is more suitable for growing young stock to reach a body weight of 182 kg. The carrying capacity of the rangeland for cattle and goat is 32 head yr-1 is 320 head yr-1, respectively. Key ecological recommendations include increasing the proportion of legumes through reseeding, implementing rotational grazing, and monitoring and controlling Cyperus rotundus to prevent competitive exclusion of forage species.
Carbon and biodiversity responses to fire and timber harvesting in dry dipterocarp community forest in Thailand PHANIN NONTHAKHOT; CHATTANONG PODONG
Biodiversitas Journal of Biological Diversity Vol. 26 No. 10 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261023

Abstract

Abstract. Nonthakhot P, Podong C. 2025. Carbon and biodiversity responses to fire and timber harvesting in dry dipterocarp community forest in Thailand. Biodiversitas 26: 5081-5088. Understanding how burning and harvesting systems in tropical dry forests influence both carbon storage and biodiversity is crucial for sustainable management. This study presents the first landscape-scale assessment of above- and below-ground carbon pools and biodiversity in dry dipterocarp forest managed by community in Thailand under four management regimes: community-based prescribed fire (Ching-Pao), selective harvesting (Ching-Kep), unmanaged natural forest, and wildfire-affected areas. Forty-eight stratified plots (i.e., 12 plots per management regime, each plot measuring 20 × 20 m) were established to measure aboveground biomass, litter biomass, Soil Organic Carbon (SOC), and tree species composition. Prescribed fire plots exhibited the highest aboveground carbon (36.74 ± 2.13 t C/ha) and Shannon diversity index (H? = 3.21), significantly exceeding wildfire plots (24.11 ± 1.84 t C/ha; H? = 2.48). SOC was also greatest in prescribed fire plots (38.21 ± 2.87 t C/ha), followed by selective harvesting, unmanaged forest, and wildfire treatments. Beta diversity analysis (Bray–Curtis) revealed distinct community assemblages among treatments, with prescribed fire fostering fire-tolerant species such as Shorea obtusa and Dipterocarpus tuberculatus. These differences indicate that low-intensity, community-managed burns can enhance carbon sequestration and sustain higher functional and taxonomic diversity compared to unmanaged or severely burned areas. The integration of functional traits—such as thick bark, resprouting ability, and high wood density—appears to underpin the resilience and productivity of fire-managed forests. Overall, the findings provide empirical evidence supporting “fire-smart” community-based forest management as a strategy to align biodiversity conservation with climate mitigation goals in tropical dry forest landscapes.
Environmental factors influencing lek site selection in the red bird of paradise (Paradisaea rubra) in Raja Ampat, Southwest Papua, Indonesia RHAMA BUDHIANA; ARIEF HAMIDI; JARWADI BUDI HERNOWO; YUDI SETIAWAN
Biodiversitas Journal of Biological Diversity Vol. 26 No. 10 (2025)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d261041

Abstract

Abstract. Budhiana R, Hamidi A, Hernowo JB, Setiawan Y. 2025. Environmental factors influencing lek site selection in the red bird of paradise (Paradisaea rubra) in Raja Ampat, Southwest Papua, Indonesia. Biodiversitas 26: 5277-5286. Understanding the ecological determinants of lek site selection is crucial for the conservation of lek-breeding species, such as the red bird-of-paradise (Paradisaea rubra), which is endemic to Waigeo and Batanta Islands in Raja Ampat, Indonesia. This study aimed to identify the primary habitat characteristics that influence lek-site preferences in P. rubra by employing a spatially explicit, multivariate methodology. Ten environmental variables (tree diameter, tree height, lek height, lek diameter, lek angle, lek length, altitude, slope, temperature, and humidity) were documented across known lek sites, and Principal Component Analysis (PCA) was employed to address collinearity and capture ecological gradients. A multiple linear regression model was then applied to the principal components to assess their association with lek suitability (Y). Three principal components were extracted: PC? (topographic openness), PC? (microclimate), and PC? (vertical structure). Regression results identified PC? as the strongest predictor of lek suitability (B: -2.629, p: 0.002). PC? represents a topographic canopy gradient, with higher values indicating denser canopy and steeper terrain at higher elevations. The negative regression coefficient, therefore, indicates that males preferentially select leks in structurally open habitats with gentler slopes, reduced canopy density, and moderate elevations. PC? and PC? had positive but non-significant effects (p>0.1), though both suggest that lek preference may also be influenced by microclimate comfort and vertical signal projection. Lek site selection in P. rubra is strongly influenced by structural and spatial habitat features, especially canopy openness and elevation. The findings emphasize that lek selection in P. rubra is shaped by the interplay of topography, microclimate, and vertical structure. Conservation strategies should prioritize protecting ridge top forest zones and limiting forest infill from secondary growth or plantation encroachment, which may disrupt lek functionality and reduce reproductive opportunities in this iconic species.

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