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Biodiversitas Journal of Biological Diversity
Published by Universitas Sebelas Maret
ISSN : 1412033X     EISSN : 20854722     DOI : https://doi.org/10.13057/biodiv/d230231
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
The Biodiversitas Journal was first published in 2000 by the Department of Biology, FMNS, Universitas Sebelas Maret, Surakarta, Indonesia, then in 2006 it was co-published by the Society for Indonesian Biodiversity and that department; since 2017 it was also hosted by Smujo. From 2003-2012 it was accredited by DGHE, Ministry of Education, R.I., since 2014 was indexed by Scopus, where DOAJ and Google Scholar was indexed first.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 6,259 Documents
 57   58   59   60   61 
Selection of Rhizobium strain from Wonogiri, Central Java on the growth of soybean (Glycine max L.) on the sand sterile medium in greenhouse SRI PURWANINGSIH
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060305

Abstract

An experiment on the selection of Rhizobium strain from Wonogiri, Central Java on the growth of soybean (Glycine max L.) on the sand sterile medium in green house. The aim of the experiment the selection and potency of the Rhizobium strain to increase the growth of soybean. The experiment was carried out in green house condition in Microbiology Division, Research Center for Biology-LIPI with sterile sand medium. The research design was Completely Randomized Design with three replications for each treatment. The Rhizobium strains used were 1 W (isolated from bean, Vigna radiata), 2 W (isolated from soybean), 3 W (isolated from bean), 4 W (isolated from soybean), 5 W (isolated from soybean), 6 W (isolated from peanut, Arachis hypogaea), 7 W (isolated from peanut), 8 W (isolated from peanut), the controls were uninoculated with Rhizobium strain and without urea fertilizer (K1), uninoculated and with urea fertilizer equal 100 kg/ha (K2). The plants were harvested after 50 days, the variable of investigation were the dry weight of canopy, roots, nodules root, total plants, number of nodules and ‘symbiotic capacity”. The results showed that all of experiment plant which be inoculated with Rhizobium able toform nodule. Strain of 2 W (isolated from soybean) has given the best effects on the growth of soybean.© 2005 Jurusan Biologi FMIPA UNS SurakartaKey words: Rhizobium strain, Glycine max L, sand sterile
Influence of Palmitic Acid and Amino Acids Addition on Iturin A Productivity by Bacillus subtilis RB14-CS YULIAR YULIAR
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060306

Abstract

The aim of this study was to observe the influence of palmitic acid and amino acids on enhancement of iturin A productivity using Bacillus subtilis RB14-CS. The concentrations of palmitic acid examined were 0.8%, 1.6%, and 3.2%. A good yield was observed when 1.6% palmitic acid was added to Polypepton S medium. The production of iturin A increased about 18% than the control. Addition of 3.2% palmitic acid was not effective on iturin A production. It gave lower pH and slightly higher viable cell number of RB14-CS than control and the others addition concentration. Whereas the addition of 0.8% of each of the following amino acids; L-arg, L-asn , L-gln , L-glu, L-gly, Lleu,L-lys, L-trp, L-tyr, and L-val could not increase iturin A productivity, but changed the proportion of iturin A peaks. L-leu, L-val, and L-asn addition produced the highest proportion of peak 3, 4, and 1 respectively.© 2005 Jurusan Biologi FMIPA UNS SurakartaKey words: iturin A, Bacillus subtilis RB14-CS, palmitic acid, and L-amino acids.
Lipase Application of lipase technology for transesterification of fatty acid ester RINI HANDAYANI; JOKO SULISTYO
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060304

Abstract

We have reported the potency of microbial extracellular enzyme for synthesis of fatty acid ester. Further investigation was aimed to study capacity of the enzyme on bioprocess of crude palm oil by transesterification of saturated fatty acid to fatty acid ester. We have studied some lipases from culture filtrate of Candida rugosa FM-9301, Bacillus subtilis FM-9101 and Pseudomonas aerogenes FM-9201, which were preincubated in a medium containing olive oil as inducers, using a shaker under conditions that allowed for lipase production at pH 4.5-6.5 and room temperature for 5 days. Those strains shown different activities during the hydrolysis of substrates, which resulted in decreasing or increasing free fatty acids those, were liberated from media containing crude palm oil and organic solvents. The optimal transesterification condition was at temperature of 45-50°C and at pH 4.5 for C. rugosa and pH 6.0 to 7.0 for P. aerogenes and B. subtilis. Under the enzyme concentration of 50% (v/v), the transesterification was rapidly occurred, while at the concentration of 20% (v/v) the enzymatically biosynthesis required longer incubation period. The substrates incubated with C. rugosa lipase exhibited higher linoleic and linolenic acid (7.16 and 2.15%, respectively), than that of B. subtilis lipase (4.85% and 1.43%, respectively), while P. aerogenes lipase(3.73% and 1.11%, respectively).© 2005 Jurusan Biologi FMIPA UNS SurakartaKey words: transesterification, lipase, crude palm oil, fatty acid ester.
The expression of p53, Rb, and c-myc protein in cervical cancer by immunohistochemistry stain ADI PRAYITNO; RUBEN DARMAWAN; ISTAR YULIADI; AMBAR MUDIGDO
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060302

Abstract

The pathogenesis of cancer as whole (50%) is caused by gene mutation. Pathogenesis of cervical cancer has focusing on Human Papilloma Virus (HPV). Early-7 (E7) proteins of HPV shell bind the Rb tumor suppressor gene, so pRb (Rb protein) can’t express. Because of the E2F transcription factor gene can’t bound with pRb, so E2F gene are going active and help c-myc for DNA replication and to stimuli the cell cycle. E6 protein of HPV is bind to and facilitates the degradation of the p53 tumor suppressor gene product. The objective of this experiment is to known the expression of p53, Rb and c-myc proteins in cancer of uterine cervix. Nineteen blocks paraffin tissue of cervical cancer are cut in thoroughly cleaned cryotome and place in glass plate that covered with poly-elysine. The immunohistochemistry is done with monoclonal antibody anti p53, Rb and c-myc proteins. The Result of this experiment is shown that the expression of proteins of p53 protein is 40%, Rb protein is 30.8% and c-myc protein is 50.1%. The conclusion from this experiment is that the expression of p53, Rb and c-myc proteins in cervical cancer are in mild category (30-70%). The experiment about cervical cancer is suggested.Key words: cervical cancer; p53; Rb; c-myc; immunohistochemistry.
Study on coloration of Monascus-nata complex using combination of rice flour, tofu solid waste, and rice bran extracts as the medium TIAS HESTI KUSUMAWATI; SURANTO SURANTO; RATNA SETYANINGSIH
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060303

Abstract

Monascus-nata complex was made up from fermented nata which was grown in the medium containing Monascus purpureus fungi, that could be used as coloration of nata. Rice flour usually used as medium to produce pigments by M. purpureus. Tofu solid waste and rice bran could be used to produce pigments although the colour intensity of M. purpureus pigment was lower than rice. The aims of these research were to study colour, pH medium and weight of miselium Monascus-nata complex from the combination of rice flour with tofu solid waste extracts and rice flour with rice bran extracts as the medium. In these research, 5% (b/v) rice flour extracts, combination of rice flour extract : tofu solid waste extract in the ratio of 1:1, 1:2, 1:3 and rice flour : rice bran extracts in the ratio of 1:1, 1:2, 1:3 were used as the medium. Ten nata de coco (1x1x1 cm3) were put in to the extracts of medium (100mL) in bottle, then inoculated with 10% (v/v) starter of M. purpureus containing 1.3x107 cfu/mL and incubated at room temperature on an orbital shaker at 100 rpm for 16 days. Parameters which were measured, i.e. colour intensity, preferable test, pH medium, weight of miselium Monascus-nata complex. Data were analyzed using analysis of variant and followed by DMRT in 5% significations except preferable test which were analyzed using hedonic test. Combination of rice flour extract : tofu solid waste extracts in the ratio of 1:1 could be used to replace rice flour extracts on the making of Monascus-nata complex. Orange colour intensity from rice flour extract : tofu solid waste extracts in the ratio of 1:1 was higher than from rice flour extracts. Orange colour intensity from rice flour extract : tofu solid waste extracts in the ratio of 1:1 and rice flour were 0.156 and 0.123 respectively. Red colour intensity from medium rice flour extract : tofu solid waste extracts in the ratio of 1:1 approach the red colour intensity of rice flour. Red colour intensity from medium rice flour extract : tofu solid waste extracts in the ratio of 1:1 and rice flour extract were 0.088 and 0.145 respectively. All of pH value decreased from 7 to 6.82. Weight of miselium Monascus-nata complex from all medium was average 0.024 g. © 2005 Jurusan Biologi FMIPA UNS SurakartaKey words: Monascus-nata complex, rice flour, tofu solid waste, rice bran
Iles-iles (Amorphophallus muelleri Blume); description and other characteristics SUMARWOTO SUMARWOTO
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060310

Abstract

The aims of the research was to asses describe and find out the others iles-iles (Amorphophallus muelleri Blume) properties on the vegetative growth and age until reach the generative growth. The research was conducted at the experimental fields of Bogor Agricultural University, Darmaga from November, 1999 to May, 2003 and at the experimental field of the Agricultural Technology Assesment Installation Unit, Sukabumi from February 2001 to April, 2002. It was found that iles-iles showed positive responses to shading, good drainage, and application of high manure (organic matter).The phenological study gave additional information on describing this species and also on cultivation aspects such us: planting distance, planting depth, the best time of harvest, and concentration of tuber andbulbil glucomannan.@2005 Jurusan Biologi FMIPA UNS SurakartaKeywords: Amorphophallus muelleri Blume, description, glucomannan, tuber, bulbil
Medium optimization for leaf numbers and shoot multiplication of lidah buaya (Aloe vera) by BAP and adenine supplement LAELA SARI
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060308

Abstract

Aloe vera of the Aloeaceae is originated from Canary Island (West Africa). This plant is commonly know in Indonesia and cultivated in large fields or in the house yard for many purposes, such as ornamental and medicine plant. The industries using it as the principle raw material has became more important due to the significant benefits of this plant. This study is purposed to obtain the medium optimization for leafnumbers and shoot multiplication of Aloe vera by BAP and adenine supplement. The shoot of Aloe vera was taken from green house of Biotechnology-LIPI. Shoots sterilized by clorox (sodium hypochlorite) solution 35% and 20% for 30 and 15 min. until get aseptic shoot (in vitro plants). The shoot isolated from in vitro plant into MS (Murashige and Skoog) medium in different concentration of BAP and adenine. The research used factorial Completely Randomized Design with two factors (BAP concentration: 0; 0.5; 1; 1.5; 2 mg/L and adenine concentration 0; 10; 20 mg/L) with 5 replicates. The results obtained have showed that addition 20 mg/L adenine to MS raise the numbers of leaf. The shoot multiplication has been augmented by addition of BAP 1 mg/L and adenine 20 mg/L. The results showed that BAP has a positive role in increasing shoot multiplication rate and that adenine has a synergic effect when added together with BAP.© 2005 Jurusan Biologi FMIPA UNS SurakartaKeywords: optimization, Aloe vera, BAP, adenine, in vitro tissue culture.
Isolation and Identification of Phosphate Solubilizing and Nitrogen Fixing Bacteria from Soil in Wamena Biological Garden, Jayawijaya, Papua SULIASIH SULIASIH; SRI WIDAWATI
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060307

Abstract

A study was undertaken to investigate the occurrence of phosphate solubilizing bacteria (PSB) and nitrogen-fixing bacteria (NFB) from soil samples of Wamena Biological Garden (WbiG). Eleven soil samples were collected randomly to estimate microbial population which used plate count method. The result showed that the microbial population ranged from 5.0x103-7.5x106 cells of bacteria/gram of soil and 5.0x103-1.5x107 cells of bacteria/gram of soil for PSB and NFB respectively. There were 17 isolates which have been identified till genus and species. The isolated microorganism were identified as PSB i.e. Bacillus sp., B. pantothenticus, B. megatherium, Flavobacterium sp., F. breve, Klebsiella sp., K. aerogenes, Chromobacterium lividum, Enterobacter alvei, E. agglomerans, Pseudomonas sp., Proteus sp. and as NFB i.e. Azotobacter sp., A. chroococcum, A. paspalii, Rhizobium sp., and Azospirillum sp.© 2005 Jurusan Biologi FMIPA UNS SurakartaKey words: phosphate- solubilizing bacteria, Nitrogen-fixing bacteria, Wamena Biological Garden
Vegetative growth of of Amorphophallus titanum (Becc.) Becc. at Cibodas Botanic Garden R. SHOLIHIN; R. SUBEKTI PURWANTORO
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060311

Abstract

A study on the growth of vegetative stadium of Amorphophallus titanum (Becc.) Becc. has been carried out in Cibodas Botanic Garden. Two bulbs are planted as collection plants since June second 2000. The big one, before dormant was in generative stadium and the other bulb is small one, before dormant was in vegetative stadium. The result of this study indicate that the growth of the big bulb longer than small one. The effect of different stadium before dormant cause the early growth of big bulb is longer than the small one.@2005 Jurusan Biologi FMIPA UNS SurakartaKeywords: Amorphophallus titanum, growth.
Rosa x odorata “viridiflora” (green rose) in Bali Botanical Garden: biological phenology and its propagation HARTUTININGSIH M. SIREGAR; I PUTU SUENDRA; MUSTAID SIREGAR
Biodiversitas Journal of Biological Diversity Vol. 6 No. 3 (2005)
Publisher : Society for Indonesian Biodiversity

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/biodiv/d060309

Abstract

Rosa x odorata “viridiflora” (green rose) was one of rose collection in Bali Botanical Garden. This shrub had shiny, dark green leaves and sprays of rossete-shaped, double flowers, 4-5 cm across, green ageing to purplish green, with narrow petals that resemble sepals. Phenology flower research was begin from the growth phase and the development until the flower was fall, every phase was identified with one or more changing. Vegetative reproduction should take over for propagation purpose. Vegetative propagation was done by cutting about 15 cm long and 0.8-1.0 cm diameter. The study was carried out in the greenhouse of Bali Botanic Garden by using CompletelyRandomized Design with 5 treatments: rootone F powder, atonik 1 mL/L, atonik 2 mL/L, IBA 1 mg/L, NAA 1 mg/L and control. Each treatment was done 3 replication had 5 pieces of cutting. The result of research indicated that cutting more responsive to rootone F with highest cutting persentage (66,67%), highest to buds (25 days after planting) and to fast flowering (147 days after planting). Green rose is one of more flowers is not interested by pollinator insect.@2005 Jurusan Biologi FMIPA UNS SurakartaKeywords: green rose, Rosa x odorata “viridiflora”, phenology.

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