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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
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Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 545 Documents
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Teratogenic test of Pandanus conoideus var. yellow fruit extract to development of rat embryo (Rattus norvegicus) LINTAL MUNA; OKID PARAMA ASTIRIN; SUGIYARTO SUGIYARTO
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020304

Abstract

Abstract. Muna L, Astirin OP, Sugiyarto. 2010. Teratogenic test of Pandanus conoideus var. yellow fruit extract to development of rat embryo (Rattus norvegicus). Nusantara Bioscience 2: 126-134. This experiment was performed to examine the effect of Pandanus conoideus Lam. var. yellow fruit extract on the percentage of the living fetus, the death intrauterus, heavy and long of fetus, fetus morphology, and skeleton structure of fetus. The experiment was used by 25 pregnant mice that randomly were divided into 5 groups, they contained 5 mice. Each group was given with a different dose. The P1 group (control) was given 1 mL sesame oil, the group P2, P3, P4 and P5 were respectively given the yellow fruit extract 0,02 mL, 0,04 mL, 0,08 mL and 0,16 mL. The P. conoideus var. yellow fruit extract was given orally on day 5 to 17 of gestation (organogenesis period). Observation was carried out on day 18 of gestation by cesarean section to take the fetus from the uterus. Fetus morphology was observed after taking fetus from uterus, whereas observation of skeleton structure was made wholemount preparation with dual colorization, they are Alcian blue and Alizarin Red-S. The result was analyzed with one way ANOVA. Results showed that giving yellow fruit extract didn't influence to the percentage of the living fetus, the death intrauterus, heavy and long of fetus. The effect of giving yellow fruit extract to the maternal was abnormality skeleton (lordosis) of fetus in the dose of 0.16 mL and obstacles to the ossification of fetus.
Expression of vimentin protein and neurofilament on forelimb buds of black-6 mice on gestation day 12 induced by 2-methoxyethanol by RT-PCR YULIA IRNIDAYANTI
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020302

Abstract

Abstract. Irnidayanti Y. 2010. Expression of vimentin protein and neurofilament on forelimb buds of black-6 mice on gestation day 12 induced by 2-methoxyethanol by Real-Time RT-PCR. Nusantara Bioscience 2: 116-120. The aim of this study was to investigate impact of 2-methoxyethanol, a major industrial chemical of plastic. Gene expression analysis is increasingly important in biological research, while real-time reverse transcription PCR (RT-PCR) is becoming the method of choice for high-throughput and accurate expression profiling of selected genes. Pregnant black-6 mice were injected intraperitoneally to 7.5 mmol/kg of 2- methoxyethanol on gestation day (GD) 10. Embryo was obtained on gestation day 12. Forelimb buds of embryo were collected and then put in the tube, which containing RNA-latter solution. To identify gene expression changes in forelimb bud caused induction 2-methoxyethanol, Real-Time PCR was used in this research. For the experiments, the real-time RT-PCR Light Cycler technology was used. The results suggested that injection of 2- methoxyethanol, in prenatal period especially on gestation day 12, the expression of vimentin in forelimb buds of mice treatment increase than control mice. Meanwhile, the expression of neurofilament tended to decrease, indirectly is not caused by the injection of 2- methoxyethanol.
Induced mutations by gamma ray irradiation to Argomulyo soybean (Glycine max) variety DIANA SOFIA HANAFIAH; TRIKOESOEMONINGTYAS TRIKOESOEMONINGTYAS; SUDIRMAN YAHYA; DESTA WIRNAS
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020303

Abstract

Abstract. Hanafiah DS, Trikoesoemaningtyas, Yahya S, Wirnas D. 2010. Induced mutations by gamma-ray irradiation to Argomulyo soybean (Glycine max) variety. Nusantara Bioscience 2: 121-125. Induced mutation by gamma-ray irradiation is one way to increase genetic variability of plants. This research used gamma-ray irradiation on low doses (micro mutation). The aim of this research was to know the response of doses level by micro mutation on gamma-ray irridation to the growing and development of Argomulyo variety of soybean [Glycine max (L) Merr]. The seeds were irradiated by gamma-ray micro mutation doses, namely 0 gray, 50 gray, 100 gray, 150 gray, and 200 gray. Variations that were obtained of each character at generation M1 and M2 influences plants growth and development either through qualitative and quantitative that finally will influence plant’s production. The average highest genetic variation at M2 generation of soybean was on 200 Gray doses. Results of the research indicated that gamma-ray irradiation on 200 Gray doses effectively caused by plant variation genetic.
Histological study of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura larvae YAYAN SANJAYA; DADANG MACHMUDIN; NANIN DIAH KURNIAWATI
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020305

Abstract

Abstract. Sanjaya Y, Machmudin D, Kurniawati ND. 2010. Histological study of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura larvae. Nusantara Bioscience 2: 135-140. The effect of SlNPV infection on body weight and peritrophic membrane damage of Spodoptera litura Fab. larvae has been carried out. The method was used Probit analysis, and based on LD 50 the virus was infected to know body weight and post-infection damage.The damage of histological structure caused by SlNPV (0, 315, 390, 465, 540 dan 615 PIB/mL) was investigated after 0, 12, 24, 72 and 96 hours post infection. The histological material was prepared by using paraffin method after fixation with Bouin Solution, then slice into 7 um and colored with Hematoxylin-Eosin. The result showed that the exposure SlNPV cause decreasing food consumption especially on 540 PIB/mL give average rate as amount of 0.1675 mg. The descriptive observation on structural intact of peritrophic membrane histology caused by SlNPV infection shows a tendency to decrease, while in control, there was no damage at all. The longer the exposition of virion in the midgut lumen the more damage on peritrophic membrane occurred. The severest damage occurred 96 hours after infection. The result proves that haNPV virion can destroy histological structure of midgut.
Hitherto unreported Agaricus species of Central India ALKA KARWA; MAHENDRA K. RAI
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020306

Abstract

Abstract. Karwa A, Rai MK. 2010. Hitherto unreported Agaricus species of Central India. Nusantara Bioscience 2: 141-145. Melghat forest region from Central India was surveyed for occurrence of medicinal and culinary mushrooms during the years 2005-2008. Out of total 153 species, ten species of Agaricus were recorded from different localities. Of these, seven species namely Agaricus bitorquis, A. subrufescens, A. augustus, A. placomyces, A. essettei, A. basioanolosus and Agaricus sp. nov (a new species) are being reported for the first time from the region. The commercial button mushroom Agaricus bisporus lacks good breeding characters due to its bisporic nature. These wild cousins of the button mushroom can definitely prove to be a good source of genetic manipulations to the existing strains and also to develop new strains with improved characters.
Diversity of secondary metabolites from Genus Artocarpus (Moraceae) ALIEFMAN HAKIM
Nusantara Bioscience Vol. 2 No. 3 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020307

Abstract

Abstract. Hakim A. 2010. The diversity of secondary metabolites from Genus Artocarpus (Moraceae). Nusantara Bioscience 2:146-156. Several species of the Artocarpus genus (Moraceae) have been investigated their natural product. The secondary metabolites successfully being isolated from Artocarpus genus consist of terpenoid, flavonoids, stilbenoid, arylbenzofuran, neolignan, and adduct Diels-Alder. Flavonoid group represents the compound which is the most found from Artocarpus plant. The flavonoids compound which is successfully isolated from Artocarpus plant consists of the varied frameworks like chalcone, flavanone, flavan-3-ol, simple flavone, prenylflavone, oxepinoflavone, pyranoflavone, dihydrobenzoxanthone, furanodihydrobenzoxanthone, pyranodihydrobenzoxanthone, quinonoxanthone, cyclopentenoxanthone, xanthonolide, dihydroxanthone.   
Effect of various sugar solution concentrations on characteristics of dried candy tomato (Lycopersicum esculentum) WAWAN BUNTARAN; OKID PARAMA ASTIRIN; EDWI MAHAJOENO
Nusantara Bioscience Vol. 2 No. 2 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020201

Abstract

Abstract. Buntaran W, Astirin PA, Mahajoeno M. 2009. Effect of various sugar solution concentrations on characteristics of dried candy tomato (Lycopersicum esculentum). Nusantara Bioscience 2: 55-61. The aims of the research were to study the effects of sugar syrup concentration on dried candy tomato characteristics and to determine the proper sugar solution concentration that gives the best characteristics of dry candy tomatoes. The research used Randomized Block Design Method with four treatments and six times repetitions. The treatment that be used was immersing the tomato in sugar solution, with concentration of A (40%), B (50%), C (60%), and D (70%) group in 18 hours. The variables measured were water content, ash, vitamin C and organoleptic tests include flavor, color, flavor and texture test. Data were analyzed using ANOVA test (Analysis of Variance) followed by DMRT (Duncan Multiple Range Test). The result showed that sugar solution concentration had different effect on water content, ash content, vitamin C content, texture, and organoleptic test for color, taste, and flavor of the dry candy tomato. The best characteristics of dry tomato candy was obtained on A (40%) group, with water content of  24.20%, ash content of 0.62%, and vitamin C content of 31.15 mg/100 g.  Standard quality of water content for dry fruit candy was maximal 25% (SII No.0718-2003) and maximally allowed ash content for food materials was 1.0% (SII 0272.90).  Vitamin C content was not much decreased compared with ripe tomato, i.e. 30-40 mg/100. Organoleptic tets result indicated that A (40%) group get the highest score, i.e. 3,98 for taste, 3,89 for flavor, and 3,98 for color.
The effect of coconut water and naphthalene acetic acid (NAA) application on the in vitro growth of Paraphalaeonopsis serpentilingua from West Kalimantan MUKARLINA MUKARLINA; AGUSTINA LISTYAWATI; SRI MULYANI
Nusantara Bioscience Vol. 2 No. 2 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020202

Abstract

Abstract. Mukarlina, Listiawati A, Mulyani S. 2010. The effect of coconut water and naphthalene acetic acid (NAA) application on the in vitro growth of Paraphalaeonopsis serpentilingua from West Kalimantan. Nusantara Bioscience 2: 62-66. The ‘Ekor tikus’ orchid (Paraphalaenopsis serpentilingua J.J. Sm.) is an epidemic orchid in West Kalimantan. Now this orchid is facing a great conservation problem and threatened to be in extinction due to human exploitation. This research was conducted to find out the in vitro growth effect of P. serpentilingua by supplementation of NAA and coconut water in culture medium. The experiment was carried out using a Completely Randomized Design with two factors and five replicates. The result showed that supplemented NAA and coconut water on MS medium affected the emergence timing of buds, the average buds number, the average leaf number/buds, percentage of buds, the emergence timing of root, average root number and percentage of root. Medium that supplemented with 1.5 ppm NAA and 10% coconut water showed the fastest emergence timing of apical bud that is 13 days after planting. Medium supplemented with 0.5 ppm of NAA and 7.5% of coconut water shown the highest average number of bud was 11 buds.
Evaluation of uniformity, variability, and stability of agronomic traits of doubledd haploid rice lines resulting from anther culture PRIATNA SASMITA
Nusantara Bioscience Vol. 2 No. 2 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020203

Abstract

Abstract. Sasmita P. 2010. Evaluation of uniformity, variability, and stability of agronomic traits of doubled haploid rice lines resulting from anther culture. Nusantara Bioscience 2: 67-72. The formation of doubled haploid lines in anther culture aims to accelerate the acquisition of pure lines. Selection of the desired traits can be done directly to the progeny of anther culture results at early generations. This experiment aims to determine agronomic traits, uniformity, and stability of the doubled haploid lines, and obtain the putative doubled haploid lines as the material for further evaluation to obtain expected lines. The first experiments used completely randomized design which was repeated five times. The treatments were 111 doubled haploid lines of first generation of anther culture results (DH1). The second experiment used split-plot design with the main plot treatments were doubled haploid lines resulting from anther culture and the subplot treatment was the second generation of doubled haploid lines (DH2) until the fifth generation (DH5). The results show that each plant within the same line have uniform agronomic traits, while the plants between different lines have different agronomic traits. The results of further evaluation on three out of 111 doubled haploid lines derived from the second to fifth generations show no difference between generations for each trait of the same lines. The results also show that the agronomic traits of the doubled haploid line were stable from generation to generation.
Effect of seaweed extracts on growth and yield of rice plants SUNARPI SUNARPI; AHMAD JUPRI; RINA KURNIANINGSIH; NUR INDAH JULISANIAH; ALUH NIKMATULLAH
Nusantara Bioscience Vol. 2 No. 2 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020204

Abstract

Abstract. Sunarpi, Jupri A, Kurnianingsih R, Julisaniah NI, Nikmatullah A 2010. Effect of seaweed extracts on growth and yield of rice plants. Nusantara Bioscience 2: 73-77. Application of liquid seaweed fertilizers on some plant species has been reported to decrease application doses of nitrogen, phosphorus, and potassium on some crop plants, as well as stimulating growth and production of many plants. It has been reported that there are at least 59 species of seaweeds found in the coastal zone of West Nusa Tenggara Province, 15 of those species weres able to stimulate germination, growth, and production of some horticultural and legume plants. This research aims to investigate the effect of seaweed extracts obtained from ten species on growth and production of rice plants. To achive the goal, seaweed (100 g per species) wasextracted with 100 mL of water, to obtain the concentration of 100%. Seaweed extract (15%) was sprayed into the rice plants during vegetative and generative stages. Subsequently, the growth and yield parameters of rice plants were measured. The results showed that extracts of Sargassum sp.1, Sargassum sp.2, Sargassum polycistum, Hydroclathrus sp., Turbinaria ornata, and Turbinaria murayana, were able to induce growth of rice plants. However, only the Hydroclathrus sp. extract could enhance both growth and production of rice plants.

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