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Nusantara Bioscience
Published by Universitas Sebelas Maret
ISSN : 20873948     EISSN : 20873956     DOI : https://doi.org/10.13057/nusbiosci
Core Subject : Education,
Other
Aims and Scope Nusantara Bioscience (Nusantara Biosci) encourages submission of manuscripts dealing with all aspects of biological sciences that emphasize issues germane to biological and nature conservation.
Arjuna Subject : Umum - Umum
Articles 545 Documents
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Isolation and identification of lactic acid bacteria from abalone (Haliotis asinina) as a potential candidate of probiotic SARKONO SARKONO; FATURRAHMAN FATURRAHMAN; YAYAN SOFYAN
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020106

Abstract

Abstract. Sarkono, Faturrahman, Sofyan Y. 2010. Isolation and identification of lactic acid bacteria from abalone (Haliotis asinina) as a potential candidate of probiotic. Nusantara Bioscience 2: 38-42. The purpose of this study was to isolate, select and characterize lactic acid bacteria (LAB) from abalone as a potential candidate probiotic in abalone cultivation system. Selective isolation of LAB performed using de Man Rogosa Sharpe medium. LAB isolate that potential as probiotics were screened. Selection was based on its ability to suppress the growth of pathogenic bacteria, bacterial resistance to acidic conditions and bacterial resistance to bile salts (bile). Further characterization and identification conducted to determine the species. The results showed that two of the ten isolates potential to be developed as probiotic bacteria that have the ability to inhibit several pathogenic bacteria such as Escherichia coli, Bacillus cereus dan Staphylococcus aureus, able to grow at acidic condition and bile tolerance during the incubation for 24 hours. Based on the API test kit, both isolate identified as members of the species Lactobacillus paracasei ssp. paracasei.
Productivity of sugarcane plants of ratooning with fertilizing treatment A. SUTOWO LATIEF; RIZAL SYARIEF; BAMBANG PRAMUDYA; MUHADIONO MUHADIONO
Nusantara Bioscience Vol. 2 No. 1 (2010)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n020107

Abstract

Abstract. Latief AS, Syarief R, Pramudya B, Muhadiono. 2010. Productivity of sugarcane plants of ratooning with various fertilizing treatments. Nusantara Bioscience 2: 43-47. This research aims to determine the sugarcane plants of ratooning productivity with low external input of fertilization treatment towards farmers can increase profits. The method used is the Completely Randomized Block Design (CRBD) with four treatments and three repetitions (4x3). Sugarcane varieties R 579 planted in each patch experiment 5x5 m2. Dosage of fertilizer: P0 = 3.6 kg/year plot experiment was 100% dosage usage of chemical fertilizers used by farmers. Further dosages were P1 (75%) = 2.7 kg/plot, P2 (50%) = 1.8 kg/plot and P3 (0.25%) = 0.9 kg/plot, each supplemented with fertilizer 5 mL of liquid organic/patch a year. Sugarcane crops with a variety of treatment showed no significant difference. The highest productivity was achieved at dosages of P2 (50% chemical fertilizers plus organic fertilizer) is 21.67 kg per square meter. Chemical fertilizers can be saved 7 quintals per hectare a year or Rp 997,500 per year. Additional costs of liquid organic fertilizer Rp. 100,000 per hectare year and labor Rp 100,000 per hectare, so the additional advantage of saving farmers fertilizer Rp. 797,500 per year.
Linoleic and linolenic acids analysis of soybean tofu with Rhizopus oryzae and Rhizopus oligosporus as coagulant CICIK SUDARYATININGSIH; SUPYANI SUPYANI
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010302

Abstract

Abstract. Sudaryatiningsih C, Supyani. 2009. Linoleic and linolenic acids analysis of soybean tofu with Rhizopus oryzae and Rhizopus oligosporus as coagulant. Nusantara Bioscience 1: 110-116. The aims of this research are to know the potency of Rhizopus oligosporus and Rhizopus oryzae as a coagulant in tofu processing for increasing the amount of linoleic and linolenic acids, and to know the time that needed by R. oligosporus and R. oryzae for increasing the amount of linoleic and linolenic acids. It uses PDA for inoculating fungi, and it is done at Sub-Lab Chemistry, Central Laboratory for Mathematics and Natural Sciences, Sebelas Maret University, Surakarta. The tofu making was done in “Dele Emas” Tofu Factory, Surakarta. Analysis of linoleic and linolenic acids was done by Gas Chromatography, in LPPT-UGM Yogyakarta. The conclusion of this research is R. oligosporus dan R. oryzae having a potency as a coagulant in tofu processing for increasing the amount of linoleic and linolenic acids. R. oryzae needs 18 hours to coagulate the tofu, and R. oligosporus requires 12 hours for the same process. R. oryzae obtained the highest amount of linoleic and linolenic acids. R. oryzae at 6 hours of fermentation (0.26% and 0.14%), and 24 hours of fermentation by R. oligosporus (0.06% and 0.04%).
Activity of Zymomonas mobilis on ethanol products made of cashew nut apple (Anacardium occidentale) with different sources of nitrogen AKHMAD MUSTOFA; SURANTO SURANTO
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010301

Abstract

Abstract. Mustofa A, Suranto. 2009. Activity of Zymomonas mobilis on ethanol production made of cashew nut apple (Anacardium occidentale) with different sources of nitrogen. Nusantara Bioscience 1: 105-109. This research is aimed at identifying Zymomonas mobilis in producing ethanol through batch fermentation process (in 24, 48 and 72 hours) using cashew nut apple extract (red, green and yellow variety) and urea, ammonium sulfate, extract of green peanut sprout and extract koro (Mucuna pruriens) as sources of nitrogen. The research showed that green cashew nut extract with ammonium sulfate in 24 hours of fermentation produced ethanol in optimum result. This treatment had pH of 5.87, 7.64 g/100 mL of sugar (with 48.44% of consumption), 8.0x107 amount of bacterium (µ = 0.154) and production of ethanol equal to 33.02 g/L (Ye = 90.19%).
Slaughter weight and carcass of male New Zealand White rabbits after rationing with koro bean (Mucuna pruriens var. utilis) URIP SANTOSO; SUTARNO SUTARNO
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010303

Abstract

Abstract. Santoso U, Sutarno. 2009. Slaughter weight and carcass of male New Zealand White rabbits after rationing with koro bean (Mucuna pruriens var. utilis). Nusantara Bioscience 1: 117-122. The objectives of the research were to know the effects of koro bean (Mucuna pruriens var. utilis) present on slaughter weight and carcass of rabbits and to know the optimum dosage that resulted in the best slaughter weight and carcass. The research used Randomized Block Design whereas 25 heads of six weeks old rabbits with 450-1270 g of body weight were divided into five groups according to the body weight. Each group was treated with different treatment. The treatment was unpresent of M. pruriens as a control (R0) and various percentage of M. pruriens as much as 21.5%, in the ration with treatment as follows: R1 (raw), R2 (heating), R3 (boiling), and R4 (fermentation). The parameters observed were slaughter weight, carcass weight, meat weight, bone weight, and adipose tissue weight. The data analyzed by analysis of variance (ANOVA) followed with Duncan's Multiple Range Test (DMRT). The presence of  processed M. pruriens could increase production of slaughter weight better than the presence of unprocessed M. pruriens. The additional of 21.5% of fermented M. pruriens resulted in the best production of slaughter weight and carcass of rabbits.
Alternative supplementary biochemic food for growing up the fresh water lobster (Cherax quadricarinatus) EDI PRIYONO; OKID PARAMA ASTIRIN; PRABANG SETYONO
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010304

Abstract

Priyono E, Astirin OP, Setyono P. 2009. Alternative supplementary biochemic food for growing up the fresh water lobster (Cherax quadricarinatus). Nusantara Bioscience 1: 123-130. This research denotes to know the influence of biochemic composition to the rapid grow of fresh water lobster on the stadium of post larva (PL) of 60 within three months. This research used the complete random planning dealing with 4 treatments and each treatment would get 3 times cycle. The treatments cover, group K tested animal was treted with 100% mill food containing 30% of protein. Group A is given with food and biochemic food containing 13,34% of protein. Group B is the treated with mill food which is mixed with biochemic food containing 10,7% of protein. While group C was tested by treating them with mill food and biochemic food containing 13,58%. After all the above mentioned would be set up within 3:1 comparation. The variable of this research were the length of the abdomen, cephalothorax, total length, and the wet weight. The data analysis is using ANOVA system on 95% power test completed by of SPSS version 13. The result of the research shows that mentioned treatments give us the same influence toward the growth of fresh water lobster. The composition of biochemic food with the containing protein around 10,7%,13.34% and 13,38% has given the same effect to the lobster growth on post larva 60 level. There is strong correlation between abdomen and cephalothorax and between the total length and the lobsters weight.
Variation of morphology, isozymic and vitamin C content of dragon fruit varieties BANATI RAHMAWATI; EDWI MAHAJOENO
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010305

Abstract

Rahmawati B, Mahajoeno E. 2009. Variation of morphology, isozymic and vitamin C content of dragon fruit varieties. Nusantara Bioscience 1: 131-137. The aims of the research was to study the variation of morphology, the band pattern of isozyme, and vitamin C content of dragon fruit (Hylocereus spp.) varieties such as super red, red and white from Pasuruan (East Java), Sukoharjo and Klaten (Central Java), and Bantul districts (Yogyakarta). Morphological character were carried include fruit, stem, and flowers of each variety of dragon fruit. The isozymic pattern was analyzed using NTSYS 2.02i. The data matrix was counted based on the DICE coefficient. The clustering was done by applying UPGMA which counted through SHAN. Vitamin C content measured by titration method then analyzed descriptively. The results showed that the higher vitamin C content was found from super red of Pasuruan (6.00) and then followed by red color (5.376) and super red (5.113) both from Bantul. The morphological variation on the stem and petal colors, and fruits were also shown by the isozymic data of three varieties of dragon fruits collected from four separated locations. Esterase (EST) showed 18 bands and forming four (4) groups based on 75% genetic similarity index. The specific band occurred on Rf 0.633 of red varieties of dragon fruit from Bantul and on Rf 0.755 from Pasuruan. The specific band also occurs on Rf 0.347 of white variety from Bantul and on Rf 0.510 and on Rf 0.633 from Klaten. Glutamic oxaloacetic transaminase (GOT) enzyme shows 12 bands and also forming four groups with a little difference for member in the fourth group. The specific band occurs on Rf 0.321 of red color fruit from Pasuruan. The specific band also occurs on the white from Pasuruan on Rf 0.446 and on Rf 0.482. The variation of dragon fruits were also supported by isozymic data indicated that the morphological character were in accordance with the genetics data.
Blood cholesterol levels of hypercholesterolemic rat (Rattus norvegicus) after VCO treatment MARTI HARINI; OKID PARAMA ASTIRIN
Nusantara Bioscience Vol. 1 No. 2 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010201

Abstract

Abstract. Harini M, Astirin OP. 2009. Blood cholesterol levels of hypercholesterolemic rat (Rattus norvegicus) after VCO treatment. Nusantara Bioscience 1: 53-58. This study aims to determine the treatment effect of VCO on blood cholesterol levels in hypercholesterolemic white rats (Rattus norvegicus L.). This study used 25 male rats of Wistar strain divided into five treatment groups, namely: control, simvastatin (1.3 mL/270 g BW), cholesterol (9:1 lard), VCO 1 (1 mL/270 g BW), and VCO 2 (1.3 mL/270 g BW). Treatment was given orally. Total cholesterol, LDL, and HDL cholesterol levels were measured on days 1, day 14, and 28. Cholesterol data (total cholesterol, LDL, and HDL) were analyzed by ANOVA and followed by a contrast test at a significance level of 5%. The results showed that treatment of VCO at different doses significantly affected the decrease in blood total cholesterol blood LDL levels, increasing blood HDL in the hypercholesterolemic white rat.
Kimpul (Xanthosoma spp.) characterization based on morphological characteristic and isozymic analysis NURMIYATI NURMIYATI; SUGIYARTO SUGIYARTO; SAJIDAN SAJIDAN
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010306

Abstract

Abstract. Nurmiyati, Sugiyarto, Sajidan. 2009. Kimpul (Xanthosoma spp.) characterization based on morphological characteristic and isozymic analysis. Nusantara Bioscience 1: 138-145. This research is aimed: (i) to know the variety of kimpul (Xanthosoma spp.) based on morphological characteristics and isozymes analysis; (ii) to know the correlation between its genetic space based on morphological characteristics and its genetic resemblance based on isozymes-banding pattern. These research results were analyzed and described by descriptive qualitative methods. Morphological observation was carried out in sub-District of Galur, Lendah, and Girimulyo, Kulonprogo District, Yogyakarta. Morphological data of the kimpul plant was explored descriptively and then made dendrogram. Data of isozymic banding pattern were analyzed quantitatively based on the appearance of the band on the gel, and qualitatively based on the thickness of the band formed, and then made dendrogram. The correlation, between its genetic distance based on morphological characteristics and its genetic resemblance based on isozymes-banding pattern, were then analyzed grounded on coefficient correlation between product-moment and goodness of it criteria based on correlation. The results pointed out that morphologically, on eight observed samples which were consist of four different types (species), each Xanthosoma from different locations did not indicate obvious differences. Esterase was formed four different banding-patterns, Glutamate Oxaloacetate Transaminase indicated eight different banding-patterns, and Peroxidase indicated seven different banding-patterns. Correlation between morphological data and data from EST and GOT isozymic banding pattern was very good (0.967918 and 0.937113), While, the correlations between morphological data and POD isozymes were good (0.892721). goodness of it criteria based on correlation. The results pointed out that morphologically, on eight observed samples  which were consist of four different types (species), each Xanthosoma from different locations did not indicate obvious differences. Esterase was formed four different banding- patterns, Glutamate Oxaloacetate Transaminase indicated eight different banding-patterns, and Peroxidase indicated seven different banding-patterns. Correlation between morphological data and  data from EST and GOT isozymic banding pattern  were very good (0.967918 and 0.937113), While, the  orrelations between morphological data and POD isozymes were good (0.892721).
Traditionally utilization of Selaginella; field research and literature review AHMAD DWI SETYAWAN
Nusantara Bioscience Vol. 1 No. 3 (2009)
Publisher : Smujo International

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.13057/nusbiosci/n010307

Abstract

Abstract. Setyawan AD. 2009. Traditionally utilization of Selaginella; field research and literature review. Nusantara Bioscience 1: 146-158. The aims of this research were to find out traditional usage of Selaginella in medication and its other usages, especially in Java and other Indonesian Archipelago. About 200 of 700-750 world species of Selaginella was found in Indonesian Archipelago. Field research and literature review indicated that Selaginella is used traditionally to heal wound, bloody stools, internal hemorrhoid bleeding, menstrual and uterine disorder, blood expediting, enhancing body endurance and longevity of live, headache, etc. Besides that, some of Selaginella are also used as raw dishes vegetable, ornamental pants, and crafts materials. The utilization of Selaginella is very limited against the amount of species and medicinal potency, it is needed an advance study on ethnobotany and phytochemistry to improve their uses.

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