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Microbiology Indonesia

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Published by Perhimpunan Mikrobiologi Indonesia

ISSN : 19783477 EISSN : 20878575 DOI : -

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology

Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.

Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan

Articles 398 Documents

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Lipase Activity Enhancement of KC4J Mutant from Oil Palm Waste Using Response Surface Method and Characterization. Aris Indriawan; Trismilah .; Wibowo Mangunwardoyo; Dadang Suhendar
Microbiology Indonesia Vol. 16 No. 1 (2022): March
Publisher : Indonesian Society for microbiology

Lipase has an important role in industry. The KC4J mutated isolates from oil palm waste had 100% similarity to Aspergillus fumigatus strain RA204, a fungus known produce lipase. The study aimed to increase lipase activity of KC4J mutant through media optimization using Response Surface Methodology (RSM) and partial characterization. The three variables of media composition (olive oil, soy flour, and pH were optimized using Central Composite Design (CCD). The lipase characterization measured the influence of pH, temperature and metal ions. The pH tested on range 6 to 12, while the temperature variation tested on 30 to 70 Â°C. The metal ions tested were Mg2+, Ca2+, Zn2+, Mn2+, Fe2+ and K+ with concentrations of 1 mM and 10 mM. The production medium containing 1.25% of olive oil, 3.5% of soy flour and 7.5 pH resulting 11.25 U/mL of Lipase activity, which was higher than the previous media composition (10.00 U/mL). The results of CCD and quadratic analysis showed that the source of carbon, nitrogen and pH had an effect on lipase activity which showed R2 0.93. The optimum lipase activity produced at pH 6 and on 60 Â°C, and the lipase stable at pH 6-8 and on 30-70 Â°C. All metal ions tested were able to increase lipase activity with Ca 2+ ion gave the highest result. Keywords: Lipase, KC4J mutant, Central Composite Design, Oil Palm Waste.

Pretreated Sugarcane Bagasse Result in more Efficient Degradation by Streptomyces sp S2 Stanislaus Aditya Agung; Rismawati .; Dede Heri Yuli Yanto; Anja Meryandini; Titi Candra Sunarti
Microbiology Indonesia Vol. 16 No. 1 (2022): March
Publisher : Indonesian Society for microbiology

Streptomyces genera plays an important role in lignocellulose degradation. Many research found that Streptomyces have cellulolytic and ligninolytic enzymes that are sufficient to degrade lignocellulosic materials. However, a minimum lignocellulosic material condition that can efficiently be degraded by sp. has Streptomyces not been fully understood. In this research, three pretreatment conditions (physical, alkaline-hydrothermal, and hydrogen-peroxide chemical treatments) of sugarcane bagasse were used as lignocellulosic material to be further degraded by Streptomyces sp. S2. Lignocellulose component measurement concluded that raw (physically treated only) bagasse was not efficiently degraded byÂ Streptomyces sp S2. Hydrogen-peroxide was effective in reducing both syringic and guaiacyl lignin. Meanwhile, alkaline-hydrothermal pretreatment was very effective in lowering syringic lignin. This study suggests that hydrogen-peroxide pretreatment can be used in any type of lignocellulosic material, which can be further degraded by Streptomyces sp. S2. On the other hand, alkaline hydrothermal pretreatment is best suited to degrade lignocellulosic material with a high percentage of syringic lignin. Key words: Alkaline-hydrothermal treatment, Hydrogen peroxide treatment, lignocellulose,Â Streptomyces sp.Â S2, sugarcane bagasse

Bacterial Community Profiles in Tapai Singkong: a Traditional IndonesianFermented Food from Cassava Tubers Tati Barus; Andiny Ndu Ufi; Watumesa Agustina Tan; Ana Lucia Ekowati; Adi Yulandi
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Tapai singkong is one of the popular fermented foods in Indonesia, which is processed from cassava tubers (Manihot utilissima ). The bacteria present during the fermentation process de Manihot utilissima determines the quality of Tapaisingkong. However, information about the bacteria of Tapai singkong is still limited.Â Therefore, this study aimed to analyze the bacterial community of based on culturing techniques Tapai singkong and based on metagenomic sequencing with Next-Generation Sequencing (NGS) techniques. Five types of samples were Tapai singkong obtained from producers in Jakarta, Bogor, Tangerang, Band Tapai singkong ung, and Kediri-Indonesia. The bacterial community in this study was studied in from Kediri Tapai singkong because the taste was most favored by the panelists based on the hedonic test. Based on the culture technique using De Man Rogosa and Sharp Agar media, the two most abundant bacterial isolates were found. Based on the 16S rRNA gene sequence, both isolates were the same lactic acid bacteria (LAB), namely Pediococcus acidilactici DSM 20284, with 99.6% similarity. Based on metagenomic sequencing, it was found that the bacteria in the consisted of Firmicutes Tapai singkong (82%), Bacteriodetes (10%), unidentified bacteria (5%), and Verrucomicrobia (1%). The genus of Firmicutes was dominated by the LAB group, namely Pediococcus (61.23%), Weissella (4.8%), Lactobacillus (3.9%), Sporolactobacillus (2.2%), and Staphyloccocus (2.1%). The results of this study showed that the LAB group was most abundant in Tapai singkong . Therefore, the role of each LAB needs to be studied further to determine its role in the quality of Tapai singkong.

IgG subclasses identification of immunized mice sera with Dengue tetravalent DNA vaccine based on prM-E genes: Identifikasi Subkelas IgG Dari Mencit yang Diimunisasi dengan Kandidat Vaksin DNA Dengue Tetravalent Beti Ernawati Dewi; Rizka Andhitia Mentari Putri; Tjahjani Mirawati Sudiro; Fitriyah Sjahta
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Background: Dengue fever is still a serious health problem in the world. DENV consists of 11 kb of single positive-stranded RNA encoding three structural proteins and seven non-structural proteins. PrM and E proteins are the main targets of the antibody response that rich of epitopes and able to induce protective immunity. There are four DENV serotypes that have similar antigenic structures in the amino acid sequence of protein E. In our previous study, we successfully constructed a recombinant tetravalent DNA vaccine candidate consisting pUMVC4a-based expression plasmid for prM-E protein of all DENV serotypes (pUMD1, pUMD2, pUMD3 and pUMD4). It has been proved that the vaccine candidate was able to induced anti-dengue IgG as well as neutralization antibody to all DENV serotypes. This study aims to determine IgG subclasses of immunized mice with recombinant tetravalent DNA vaccine candidates based on prM-E genes of all serotypes. Methods: Mice (Balb/c) were immunized with a dose of 100 Î¼g 100 uL/mouse in triplicate, at three weeks interval. Blood was drawn two weeks post immunization as well as termination blood. IgG subclasses titre were measured using in-house indirect ELISA. Results: The titer of IgG2a subclass was the highest levels with optical density of 1.004Â±0.154 followed by IgG1,IgG2b, and IgG3 to DENV-2, respectively. Conclusion: The data demonstrate the humoral immune response IgG subclasses of this recombinant tetravalent DNA vaccine candidates based on prM-E genes of all serotypes, supporting further translational studies to advance the development of this candidate in response to DENV infection. Keywords: dengue vaccine, DNA vaccine, recombinant, IgG subclass, Tetravalent

The Effect of Pili Protein of Klebsiella pneumoniae 65,5 kDa on Enhanced IFN- Gamma Levels in Mice Liver Dini Agustina; Zahrah Febianti; Enny Suswati; Diana Chusna Mueida; Muhammad Ali Shodikin; Samudra Ayu
Microbiology Indonesia Vol. 17 No. 2 (2023): June
Publisher : Indonesian Society for microbiology

Klebsiella pneumoniae develops antibiotic resistance by producing enzymes such as Extended-Spectrum Beta-Lactamase and Carbapenemase. Antibiotic resistance causes K. pneumoniae to have less antibiotic activity and more virulence factors. Capsule polysaccharides, lipopolysaccharide, Outer Membrane Protein, siderophores, and pili are all virulence factors in K. pneumoniae. This study aims to demonstrate the possibility of a host immunological response to the pili protein K. pneumoniae 65.5 kDa by injecting it into mice and measuring the levels of IFN-gamma cytokines in the mice's liver. This study used mice liver samples taken from 21 mice aged 6-8 weeks in the experimental investigation with a randomized post-test only controlled group design. Phosphate buffer saline was given to KI, pili protein antigen 65.5 kDa + Freunds' adjuvant was given to K2, and Freunds' adjuvant was given to K3. IFN-gamma concentration was measured using the sandwich ELISA method. The average concentration of IFN-gamma in the mice liver in this study was 247.68±47.67 pg m 'L ', 163.19±13.63 pg m'L', and 182.41 ±41.70 pg m'L'. The p-value of the Welch ANO VA test was 0.005 (p < 0.05), hence the Post Hoc Games-Howell test was used. The Games-Howell test showed a statistically significant difference in the mean value of IFN-gamma in KI compared to K2 and K3 of 0.007 and 0.046, respectively. There was no statistically significant difference between K2 and K3 with a p-value of 0.511. These findings revealed that intraperitoneal injection of Klebsiella pneumoniae pili protein 65.5 kDa did not increase IFN-gamma levels in the mice liver.

Endophytic Bacteria of Pemphis acidula on Karst Ecosystem of Gorontalo, Indonesia Yuliana Retnowati; Dewi Wahyuni K Baderan; Ramli Utina
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

Wild plant Pemphis acidula in coastal habitats is often associated with microbes on their root, stem, or leaf. The objective of the study was to reveal the endophytic bacteria associated with Pemphis acidula in the coastal area of Gorontalo, the study antibacterial activity of endophytic bacteria against pathogenic bacteria, and identify endophytic bacteria based on molecular characteristics. The Pemphis acidula sampling was conducted on three coastal areas of Gorontalo, including Biluhu beach, Dulanga beach, and Olele beach. The sample consists of the root, stem, and leaf of Pemphis acidula. The result showed that the endophytic bacteria were just found on the leaf. There were four isolates with similar morphological features. The antibacterial activity on a broad spectrum of two endophytic bacteria against both Escherichia coli and Staphylococcus aureus, whereas two isolates others on a narrow spectrum against Staphylococcus aureus. The endophytic bacterial identify as Bacillus tequilensis based on 16S rRNA gene sequence and this isolate is closely related to Bacillus tequilensis strain 20Q9-B-4-13 OK653944.1.37-1458 on a similarity index of 100%.

The qPCR Assay for Detecting The Presence and Relative Abundance of Pseudomonas aerugionosa and Antibiotic Resistance Gene aadA2 in Hospital Wastewater of National Reference Hospital Dr. Cipto Mangunkusumo (RSCM) Rida Tiffarent; Rosdiana Irawati; Conny Riana Tjampakasari; Fithriyah Sjatha; Windi Muziasari; Anis Karuniawati
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Antimicrobial resistance is one of the top 10 global health threats. The hospital wastewater (HWW) potentially becomes the reservoir and dissemination of antibiotic resistance gene (ARG) and bacterial pathogens. In Indonesia, the protocol to monitor the ARGs form HWW has not been established. This study aimed to detect the presence and find the relative abundance of P. aeruginosa and aadA2 genes from Dr. RSUPN. Cipto Mangungkusumo (RSCM) inlet and outlet wastewater through qPCR assay. The primers used were supported by Resistomap. The study revealed that the qPCR assay was able to detect the Ct value of P. aeruginosa and aadA2. The aadA2 gene was found in all waste water samples, meanwhile P. aeruginosa was only found in some of inlet samples. aadA2 had the highest relative abundance and this geneâ€™s mobility uses plasmids and integrons that potentially enhance the acquired antimicrobial resistance (AMR) mechanism. This study implicated that qPCR assay was capable to detect pathogenic bacteria and ARG, and ARG could be released to the environment even though the wastewater samples have been proceeded in wastewater treatment plants (WWTP). The qPCR assay can be used as the method to monitor the AMR status in a hospital and the spreading potency to the environment using the HWW.

Design of Adenovirus 5 Vector with Adenovirus 26 Hexon Hypervariable Region Sequence using In Silico Approach Afina Firdaus Syuaib; Ernawati Arifin Giri-Rachman; Aluicia Anita Artarini
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Adenovirus type 5 (Ad5) is one of the vaccine vectors, including the COVID-19 vaccine. Pre-existing immunity to Ad5 may suppress the immunogenicity and efficacy of adenovirus vectored vaccine. The neutralizing antibodies are directed specifically toward seven hypervariable regions (HVR) of hexon proteins located on the outer surface of the capsid. This study aims to design an Ad5 vector that may circumvent anti-Ad5 immunity by designing a chimera Ad5 vector with the sequence of Ad26 HVR (Ad5HVR26) using in silico approach. Substitution of the Ad5 HVR DNA sequence may affect the alternative splicing process of adenovirus mRNA, which then influence the protein product. The splice site prediction of Ad5HVR26 chimera vector was found at HVR5, 6, and 7. The codon change in the splice site was performed to decrease the possibility of incorrect splicing, while retaining the original amino acid sequence. The HVR substitution in chimera vector Ad5HVR26 may also affect the interaction of hexon in the capsid. The HVR2 and HVR4 hexon proteins individually interact with other hexon proteins and IX protein. Thus, two designs of the Ad5HVR26 chimera vector were created in this research. The first design was the Ad5 chimera vector with complete substitution of HVR hexon by Ad26 sequence, with codon modification on the splice site. The second design was Ad5HVR26 chimera vector without the HVR2 and HVR4 substitution to maintain the hexon protein interaction with the capsid proteins. Production of the designed vectors are needed to prove the reduction of vector neutralization by pre-existing immunity.

Effect of Cocoa Bean Fermentation Using Lactic Acid Bacteria and Yeast Starters on Flavonoid Formation and Antioxidant Activity Anja Meryandini; Irvan Anwar; Titi Candra Sunarti
Microbiology Indonesia Vol. 17 No. 1 (2023): March
Publisher : Indonesian Society for microbiology

This study investigates the effect of fermentation using lactic acid bacteria and yeast as starters on the formation of flavonoid compounds and the antioxidant activity of cacao beans. The fermentation process were divided into 4 groups: F1: spontaneous fermentation, F2: fermentation using Lactic Acid Bacteria (LAB), F3: fermentation using yeast and F4: fermentation using LAB and yeast. The extraction process was done using ethanol. Flavonoid content was analysis using spectrophotometer assay. The antioxidant activity was analyzed by 1,1-difenil-2-pikrilhidrazil (DPPH) method. All ethanol extract samples of fermented cacao beans contained alkaloids, polyphenols, flavonoids, and tannins. The flavonoid compounds from ethanol extract of cacao beans in F1 is 4.35 Â± 0.20 mg/L, F2 (5.64 Â± 0.05), F3 (5.37 Â± 0.17), and F4 (5.99 Â± 0.23 mg/L). The antioxidant activity of cacao bean fermentation extracts using starter were increase compared to the spontaneous fermentation extract (F1). The antioxidant activity in F2 increased to 46.45 Â± 2.00%, F3 (49.05 Â± 0.58%), and F4 (50.33 Â± 0.43%), while the antioxidant activity of F1 was 42.31 Â± 0.66%. IC50 value as the ability of the extract to reduce 50% DPPH radical on the ethanol extract of cacao beans from spontaneous fermentation (F1) was 141.67 mg/L. The IC50 value of the fermented cacao bean extract with the addition of starter was obtained at F2 at 109.30 mg/L, F3 (97.51), and F4 is 88.15 mg/L.

Multidrug Resistance and Extensively Drug-Resistance in Staphylococcus aureus, Staphylococcus epidermidis, and Staphylococcus haemolyticus Cliff Clarence Haliman; Dimas Seto Prasetyo; Conny R Tjampakasari; Fera Ibrahim; T Mirawati Sudiro
Microbiology Indonesia Vol. 16 No. 2 (2022): December
Publisher : Indonesian Society for microbiology

Antimicrobial resistance in bacteria has become a leading global public health issue. Staphylococcus sp. has an efficient mechanism to deal with antimicrobial agents that make them hard to treat in hospital-acquired and community-acquired infections. This study was conducted due to limited data about multidrug resistance and extensively drug resistance in Staphylococcus sp. in Indonesia. This study was a descriptive retrospective study using a cross-sectional design to get the prevalence and antimicrobial susceptibility of S. haemolyticus, S. aureus, and S. epidermidis. The data was secondary data extracted from WHONET 2022 software. This studyâ€™s data were from bacteria from samples sent to UKK LMK FKUI, Jakarta from 2017 to 2021 for routine diagnostic. In this study, we found that the prevalence of methicillin-resistant S.aureus was 24,9%, methicillin-resistant S.epidermidis was 65,5%, and methicillin-resistant S.haemolyticus was 86,8%. The prevalence of MDR S.aureus is less than S.epidermidis and S.haemolyticus, respectively. MDR S.haemolyticus was consistently above 85% each year, while S.epidermidis was above 50% and S.aureus was below 50%. XDR Staphylococcus was only found in S.aureus and S.haemolyticus, i.e. three and seven XDR isolates of S.aureus and S.haemolyticus respectively during 2017-2021. Although we could not find any pan-resistant isolates from all samples, we found methicillin-resistant S.aureus and S.haemolyticus isolates that were also resistant to vancomycin and linezolid. S.haemolyticus dan S. epidermidis were an important coagulase-negative Staphylococcus species that canâ€™t be neglected due to the high percentage of MDR and the discoveries of XDR in S.haemolyticus so that they have the potential to disseminate resistance plasmids to the more virulent bacteria. Therefore we need to control the use of antimicrobial agent to prevent this resistance.

Page 39 of 40 | Total Record : 398

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Iman Rusmana

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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Iman Rusmana

Contact Email rusmana13@yahoo.com

Phone +62217560536

Journal Mail Official microbiology.indonesia@gmail.com

Editorial Address kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location Kota tangerang, Banten INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Iman Rusmana

Contact Email
rusmana13@yahoo.com

Phone
+62217560536

Journal Mail Official
microbiology.indonesia@gmail.com

Editorial Address
kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com

Location
Kota tangerang,

Banten

INDONESIA