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kPERHIMPUNAN MIKROBIOLOGI INDONESIA (SeKretariat PERMI), Gedung 10.2 Indonesian Life Sciences Center (ILSC), Zona Bisnis Teknologi Puspiptek, Jalan Raya Serpong - Bogor Gunung Sindur, Jawa Barat 16340, Indonesia. Email: microbiology.indonesia@gmail.com
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Microbiology Indonesia
Published by Perhimpunan Mikrobiologi Indonesia
ISSN : 19783477     EISSN : 20878575     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology
Microbiology Indonesia provides a unique venue for publishing original researches in microbiology (espesially from Indonesian reseachers), and ensures that authors could reach the widest possible audience. Microbiology Indonesia publishes a wide range of research disciplines on bacteria, archaea, fungi, protozoa, and virus as well as biotechnology related to microbiology. Topics include (but are not limited to): -methods in microbiology, -bioprocess, -environmental microbiology, -food microbiology, -plant-microbe interaction, -animal-microbe interactions, -microbial community, -microbial genetics, -virology, -comparative and functional microbial genomics, -and gene expression in microbes.
Arjuna Subject : Imunologi dan Mikrobiologi (semua kategori) - Mikrobiologi dan Bioteknologi Terapan
Articles 398 Documents
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Enhanced Acetone, Butanol, and Ethanol Fermentation by Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in a Chemically Defined Medium: Effect of Iron and Initial pH on ABE Ratio HANIES AMBARSARI AMBARSARI; KENJI SONOMOTO
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2229.426 KB) | DOI: 10.5454/mi.6.4.1

Abstract

Batch studies were performed to investigate the performance of Clostridium saccharoperbutylacetonicum N1-4 (ATCC 13564) in acetone-butanol-ethanol (ABE) fermentation as affected by iron and initial pH in defined TYA (Tryptone-Yeast extract-Acetate) media. Different concentrations of FeSO4.7H2O in the TYA media were found to influence the ABE fermentation process resulting in different ABE ratios. From the experiment with different concentrations of FeSO4.7H2O, it was also found that lag phases at initial pH of 4.4 were longer than those at initial pH of 6.5, however they could still have higher ABE productivity values. Addition of 0.003 g L-1 FeSO4.7H2O could give the highest ABE production with both initial pH of 6.5 and 4.4. With more than 0.01 g L-1 FeSO4.7H2O, ratios of acetone to butanol (0.50 - 0.53) were higher at initial pH of 4.4 than those (0.26 - 0.28) at initial pH of 6.5. Those differences were not obtained with low concentration of FeSO4.7H2O among the same initial pH. It was also confirmed that initial pH affected ABE production significantly more than FeSO4.7H2O by statistical analysis.
Adaptation of Oil Palm Seedlings Inoculated with Arbuscular Mycorrhizal Fungi and Mycorrhizal Endosymbiotic Bacteria Bacillus subtilis B10 towards Biotic Stress of Pathogen Ganoderma boninense Pat YENNI BAKHTIAR; SUDIRMAN YAHYA; WAHONO SUMARYONO; MEITY SURADJI SINAGA; SRI WILARSO BUDI
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (775.433 KB) | DOI: 10.5454/mi.6.4.3

Abstract

The effects of mycorrhizal endosymbiotic bacteria Bacillus subtilis B10 and composite of arbuscular mycorrhizal fungal spores in green house experiment were examined in order to evaluate their effectiveness and compatibility with oil palm seedlings in the presence of a fungal pathogen Ganoderma boninense, the most serious pathogen in oil palm (Elaeis guineensis Jacq) in Indonesia. A three factors experiment were conducted, with mycorrhizal inoculation (M0 and M1), bacterial B. subtilis B10 inoculation (B0 and B1), and G. boninense inoculation (G0 and G1) as the first, second, and third factors, respectively. The results showed that disease severity index, plant height, root dry-weight, and phosphorus uptake were affected by co-inoculation of mycorrhizal endosymbiotic bacteria B. subtilis B10 and composite of arbuscular mycorrhizal fungi. Co-inoculation of mycorrhizal endosymbiotic bacteria B. subtilis B10 and arbuscular mycorrhizal fungi did not only reduce the percentage of basal stem rot incidence, but also significantly increased plant height and phosphorus uptake by oil palm seedlings. Our results suggest that in oil palm seedlings mycorrhizal endosymbiotic bacteria B. subtilis B10 worked synergistically with arbuscular mycorrhizal fungi in increasing plant adaptation toward biotic stress of pathogen G. boninese and could be promising biocontrol agents.
Genus Diversity of Actinomycetes in Cibinong Science Center, West Java, Indonesia YANTYATI WIDYASTUTI; PUSPITA LISDIYANTI; SHANTI RATNAKOMALA; GINA KARTINA; RONI RIDWAN; ROHMATUSSOLIHAT ROHMATUSSOLIHAT; NITA ROSALINDA PRAYITNO; EVI TRIANA; NUNUK WIDHYASTUTI; RASTI SARASWATI; RATIH DEWI HASTUTI; YULIN LESTARI; MISA OTOGURO; SHINJI MIYADOH; HIDEKI YAMAMURA; TOMOHIKO TAMURA; KATSUHIKO ANDO
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2250.014 KB) | DOI: 10.5454/mi.6.4.4

Abstract

Actinomycetes are microorganisms that play important role to support human health and  known as soil microorganisms. The aim of the research was to describe genus diversity of actinomycetes in Cibinong Science Center (CSC), West Java. Samples for isolation were soil and plant litters. The samples were air dried and ground. We employed isolation methods: dry heat (DH), sodium dodecyl sulphates-yeast extract (SDS-YE), rehydration and centrifugation (RC), and oil separation (OS). A total of 263 isolates of actinomycetes were isolated in CSC, in 2004-2006. Totally 58, 144, 50, and 11 isolates were isolated under each isolation methods, respectively. All isolates were identified using the 16S rRNA gene sequencing method. The results showed that the isolates were belonged to the family Kineosporiaceae, Micromonosporaceae, Nocardiaceae, Pseudonocardiaceae, Streptomycetaceae, Streptosporangiaceae, Mycobacteriaceae, Nocardioidaceae, Nocardiopsaceae, and Thermomonosporaceae. There were 23 genera under those families. Homology value of the isolates based on BLAST search using 16S rRNA gene sequence data as queries showed that 136, 91, 30, and 6 isolates were ≥99, 98, 97, and ≤96%, respectively, compared to the known sequence in data base. The later 6 isolates were interesting for further identification leading to new taxa. Recognized species of Streptomyces genera under the member of the Streptomycetaceae were dominant among other isolates.
The Prevalence and Subtype Distribution of Hepatitis C Virus Infection among Hemodialysis Patients in a Private Hospital in Surabaya, Indonesia MOCHAMAD AMIN; JUNIASTUTI .; TAKAKO UTSUMI; YOSHIHIKO YANO; MOCHAMAD YUSUF; MOCHAMMAD THAHA; PRIYO BUDI PURWONO; RETNO HANDAJANI; SOETJIPTO .; HAK HOTTA; YOSHITAKE HAYASHI; MARIA INGE LUSIDA
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (837.539 KB) | DOI: 10.5454/mi.6.4.5

Abstract

The prevalence of hepatitis C virus (HCV) infection has been as high as approximately 80% in patients with maintenance hemodialysis in public hospitals in Indonesia. However, the prevalence in private hospitals has not been examined yet. The aim of  this study was  to  investigate  the prevalence of anti-HCV antibody and  the subtype distribution in patients with hemodialysis in a private hospital in Surabaya, Indonesia. Sera were obtained from 41 hemodialysis patients in a private hospital in Surabaya. The positive prevalence of anti-HCV antibody was carried out by  the enzyme-linked  immunosorbent assay (ELISA). Anti-HCV-positive sera were subjected  to  reverse transcription-PCR (RT-PCR) to detect HCV RNA and then direct sequencing. The HCV subtype was examined by phylogenetic analysis. Twenty five patients (61%) out of 41 were positive for anti-HCV antibody, and HCV-RNA was detected in 19 patients. The positive prevalence of anti-HCV antibody was 7.7% (one out of 13 patients) among patients who had undergone hemodialysis  for  less  than one year, whereas  it was 85.7%  (24 out of  28  patients)  among  patients who  had  undergone  hemodialysis  for  over  one  year.  Phylogenetic  analysis revealed HCV-1a  (52.6%) was  the most common  subtype,  followed by 1b  (15.8%), 1c  (15.8%), 2a  (5.3%), and 3k (5.3%). In conclusion,  the prevalence of HCV  infection among hemodialysis patients  in a private hospital was as high as  that  in general hospitals. The predominant subtype was HCV-1a, which  is  in accordance with  the previous studies  in general hospitals  in Surabaya,  Indonesia.
Biomining: an Established and Dynamic Biotechnology DAVID BARRIE JOHNSON
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1074.211 KB) | DOI: 10.5454/mi.6.4.7

Abstract

“Biomining” is generic term to describe the application of living organisms to extract and recover metals from mineral ores and waste materials. Since its inception as a crude technology (“dump leaching”) for treating “run of mine” rocks and boulders that contained too little copper to be processed by conventional processing, engineering options used  in biomining have become  increasingly  refined and diverse. Currently, microbiological processing is used to extract both base metals (copper, and to lesser extents nickel and zinc) and precious metals (mostly gold) from ores and mineral concentrates in heaps and stirred-tank bioreactors, as well as in dumps. Recent developments  include  the demonstration, at pilot-scale, of  indrect  leaching of zinc  sulfide concentrates,  in which the biological step (regeneration of ferric iron) is carried out independently of abiotic mineral oxidation, and  using microbiologically-mediated  reductive  dissolution  of  ferric  iron minerals  to  liberate  nickel  from lateritic ores.
Optimization of Culture Conditions to Produce Thermostable Keratinolytic Protease of Brevibacillus thermoruber LII, Isolated from the Padang Cermin Hot Spring, Lampung, Indonesia DEWI SESWITA ZILDA; ENI HARMAYANI; JAKA WIDADA; WIDYA ASMARA; EKO IRIANTO; GINTUNG PATANTIS; YUSRO NURI FAWZYA
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (925.117 KB) | DOI: 10.5454/mi.6.4.8

Abstract

Hot springs  represent one of  the most promising  sources  for  the  isolation of  thermostable enzyme producers.  The microorganisms living in a hot spring not only have to withstand elevated temperatures but also extreme environmental pH and certain chemical compounds that are often toxic to other microbes. A bacterial strain denoted  as  Brevibacillus  thermoruber  LII  has  been  isolated  from  Padang  Cermin  Hot  Spring,  Lampung,  Indonesia. Optimization of  the conditions  for protease production by  this strain  revealed  that  the  isolate produced a thermostable protease optimally at temperature and pH ranges 45-55  C and 6-7, respectively, with keratin as substrate. The strain’s keratinolytic activity was shown by the ability to degrade untreated chicken feathers after 24 h  incubation  in  liquid medium.
Bacteria Associated with Arbuscula Mycorrhizal Spores Gigaspora margarita and Their Potential for Stimulating Root Mycorrhizal Colonization and Neem (Melia azedarach Linn) Seedling Growth SRI WILARSO BUDI; YENNI BAKHTIAR; NUNANG LAMAEK MAY
Microbiology Indonesia Vol. 6 No. 4 (2012): December 2012
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (654.598 KB) | DOI: 10.5454/mi.6.4.6

Abstract

Four isolates (BGi1, BGi2, BGi3, and BGi4) bacteria were isolated from surface sterilized arbuscular mycorrhizal fungi (AMF) spores of Gigaspora margarita (Gm). Based on 16S rDNA analyses and phylogenetic tree, it was revealed that isolates BGi1, BGi3 and BGi4 belong to genus Bacillus, whereas BGi2 was very close  to Bacillus megaterium EG 24. Enzymatic activity test showed that all four isolates had cellulase and protease activities; while one isolate (Bacillus sp. BGi4) also has pectinase activity in addition to the celulase  and protease activities. Dual inoculation of Melia azedarch Linn roots by B. megaterium BGi2  and AMF spores G. margarita enhanced mycorrhizal root colonization by 58.3 %. Combination of Bacillus sp. BGi1 and G. margarita increased  height, diameter, shoot biomass, and root biomass of  M. azedarch by  353, 4.8, 4546, and 2810%, respectively,  in comparison to the uninoculated control plant.
Characterization of Thermoplasma Species Cultured from Sampling on Tangkuban Perahu, Indonesia AMARILA MALIK; IMAN SANTOSO; ANDI YEHUDA; SERUNI K.U. FREISLEBEN; SEPTELIA INAWATI WANANDI; HARALD HUBER; ZESSINDA LUTHFA; ROSARI SALEH; HANS-JOACHIM FREISLEBEN
Microbiology Indonesia Vol. 8 No. 1 (2014): March 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (143.895 KB) | DOI: 10.5454/mi.8.1.3

Abstract

Archaea is an organisme with unique feature because of its ability to inhabit an extremophyle conditions. Our expeditions to Tangkuban Perahu, West Java aimed to obtain archaealstrains from the solfatara fields located in Domas crater. From the samples, we intended to culture Thermoplasma species growing around 55 °C below pH 2, which until now have not yet been fully characterized. We collected five samples from mud holes with temperatures from 52 °C to 57 °C and pH below 2. In serial cultures of up to 8 transfers in Freundt’s medium we grew tetraetherlipid synthesizing Thermoplasma species as documented by phase contrast microscope. Total membrane lipid extracts were analysed by thin layer chromatography; the pattern matched total lipid extracts from Thermoplasma acidophilum DSM 1728 membranes. For confirmation, 16S rDNA identification performing PCR and sequencing were carried-out. Analysis using BLAST showed T. acidophilum identities as the highest similarity of 99%, followed by T. volcanium, also with99% similarity (ANKF776908 and ANKF776909). This is the first report of culturing cell-wall-less thermoacidophilicarchaea,in particular Thermoplasma species in Indonesian laboratories.
Effect of Spontaneous Lactic Acid Fermentation on Physico-Chemical Properties of Sweet Potato Flour NETI YULIANA; SITI NURDJANAH; RIBUT SUGIHARTO; DEARY AMETHY
Microbiology Indonesia Vol. 8 No. 1 (2014): March 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1032.252 KB) | DOI: 10.5454/mi.8.1.1

Abstract

Native sweet potato flour is usually has low whiteness index and limited application to food systems due to its  inherent functional properties. Therefore,  it needs modification process  to  improve  this property. In  this study, sweet potatoes cubes were  lactic spontaneously fermented for 120 h before being processed  to flour  to modify  its properties. Selected physico-chemical properties of flour were then determined and compared with the control (without fermentation). The results showed that lactic acid fermentation significantly caused more changes on flour properties. The lactic acid fermentation caused an alteration in the starch granules as evident by Scanning Electron Microscopy. When compared to the control flour, spontaneous fermented flour had lower solubility, higher swelling power, and paste viscosity.  The results suggested lactic spontaneous fermentation within 120 h period of  time could provide a greater extent of  flour modification.
Klebsiella pneumoniae from Indonesian Tempeh were Genetically Different from that of Pathogenic Isolates EVELINE AYU; ANTONIUS SUWANTO; TATI BARUS
Microbiology Indonesia Vol. 8 No. 1 (2014): March 2014
Publisher : Indonesian Society for microbiology

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (300.383 KB) | DOI: 10.5454/mi.8.1.2

Abstract

Tempeh  is  important  traditional  Indonesian  fermented  food made  from  soybeans  employing Rhizopus oligosporus or R. microsporus. During the process of tempeh production, some bacteria from the environment and tempeh starter become an integral part of tempeh, and even have important roles in determining   the final quality of tempeh it self. Several studies reported the presence of Klebsiella pneumoniae in tempeh as one of vitamin B12 producing bacteria in tempeh. However, K. pneumoniae also known as opportunistic pathogens causing pneumonia and liver abscess in human. In this study, Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction  (ERIC-PCR) was  employed  to  determine  genetic  diversity  of K.  pneumoniae isolated from tempeh and compared them with medical isolates. The result indicated that isolates from tempeh were genetically distinct  from  those of medical  isolates.

Page 7 of 40 | Total Record : 398

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