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BIOTROPIA - The Southeast Asian Journal of Tropical Biology
ISSN : 02156334     EISSN : 1907770X     DOI : http://dx.doi.org/10.11598/btb
BIOTROPIA, The Southeast Asian Journal of Tropical Biology, is a scientific publication of the Southeast Asian Ministers of Education Organization (SEAMEO) – Southeast Asian Regional Center for Tropical Biology (BIOTROP). It publishes articles in the broad areas of tropical ecosystems and environmental impacts, biodiversity conservation and sustainable development and biotechnology.
Articles 520 Documents
HISTOPATHOLOGY OF THE TELENCEPHALON AND DIENCEPHALON OF T1LAPIA NILOTICA EXPOSED TO SUBLETHAL DOSE OF MALATHION S-[1,2-DI-(ETHOXYCARBONYL ETHYL) DIMETHYL PHOSPHOROTHIOLOTHIONATE] EDNA A AMPARADO
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 5 (1992)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (753.705 KB) | DOI: 10.11598/btb.1992.0.5.195

Abstract

A  35-day  exposure  of  Tilapia  nilotica  embryos  to  sublethal  doses  of  3.0  ppm  and  0.3  ppm malathion,  S-[l,2-di-(ethoxycarbonyl  ethyl)  dimethyl  phosphorothiolothionate],  commercial  grade,  EC  57, produces cellular and ultrastructure changes in the brain. A number of nuclear centers of the treated animals are markedly larger than those of the control. Aberrant features observed in day-45 embryos are the neoplastic masses and increased vascularization. Ultrastructure defects include the presence of nuclear blebs, cytoplasmic vacuolations and increased lysosomal bodies.
PREDICTING THE IMPACT OF CLIMATE CHANGE ON THE DISTRIBUTION OF Flindersia pimenteliana F. Muell. IN INDONESIAN PAPUA AND PAPUA NEW GUINEA Iyan Robiansyah
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 24 No. 1 (2017)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1261.304 KB) | DOI: 10.11598/btb.2017.24.1.556

Abstract

Population of Flindersia pimenteliana (Maple Silkwood) in Indonesian Papua and Papua New Guinea is severely fragmented and experiencing a continuing decline due to habitat destruction and illegal logging. This species is very susceptible to environmental changes and at greater risk of extinction due to its small and fragmented geographic ranges and low abundance. Using maximum entropy (MaxEnt) method, the present study predicted the impact of climate change on the distribution of the species across its native distribution area. Elevation and 19 bioclimatic variables commonly used in species distribution modeling were used as predictors.  The prediction model of the current potential distribution identified a total area of 156,214 km2 in Indonesian Papua and Papua New Guinea (18% of total land area) as suitable habitat for F. pimenteliana. Elevation and precipitation of the wettest, coldest and warmest quarters contributed most to the model. Based on the average of HadGEM2-ES and MIROC-ESM models, potential distribution projections under RCP8.5 scenario suggested a habitat gain of 16% for 2050 and 8% for 2070 in the species distribution. Whereas under RCP4.5, an average habitat gain of 7% was predicted for both 2050 and 2070. The newly suitable habitats were predicted to be found mainly in Southern and Western Highland of Papua New Guinea. Protection of these areas from habitat destruction and land use change is needed to assist F. pimenteliana find the most suitable climate for its survival.
IV. STORAGE AND GERMINATION TESTS ON SHOREA JAVANICA SEEDS M.I.J. UMBOH
BIOTROPIA Vol. 1 No. 1 (1987)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (449.986 KB) | DOI: 10.11598/btb.1987.1.1.88

Abstract

Seeds  of  Shorea  javanica  K  &  V  (Dipterocarpaceae),  were  subjected  to  different  treatments  of temperature (27 ± 2°C, 20 ± 2°C and < 10°C), coating (ash and paraffin) and relative humidity (20, 66 and 86%) and their germination capability as well as moisture content assessed after 3, 7, 14 and 30 days of storage. The germination percentage of  the seeds stored 30 days  in a  cloth bag under different  conditions of temperature showed highly significant differences  (a = 0.01). No significant differences were  found on  the effect of coating, duration of storage as well as on the interaction between these two factors. The moisture content of the seeds between 13 and 14% gave a germination percentage above 50% after 30 days.   
FUNGAL POPULATION, AFLATOXIN AND FREE FATTY ACID CONTENTS OF PEANUTS PACKED IN DIFFERENT BAG TYPES SONIA S P BULAONG
BIOTROPIA No. 19 (2002)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (1371.393 KB) | DOI: 10.11598/btb.2002.0.19.229

Abstract

Shelled peanuts of Gajah var. with initial moisture content of 7% were stored at 11 kg/bag in four bag types namely: jute bag, polypropylene bag, jute bag doubled with thin polyethylene (PE), and jute bag doubled with thick PE. Storage was done for six months under warehouse conditions with monitoring of relative humidity and temperature. Samples taken at the beginning of storage and every month thereafter were analyzed for moisture content, fungal population, aflatoxin and free fatty acid contents. Statistical analyses showed that moisture content, fungal population, and free fatty acid contents were significantly higher in jute and polypropylene bags than in PE-dou,bled jute bags. No significant differences were obtained in aflatoxin contents among bag types but at the end of six months storage, toxin level in jute bag exceeded the 30 ppb limit. Polypropylene had second highest toxin level at 23 ppb. The PE-doubled bags had 17 and 19 ppb total aflatoxins for thin and thick films, respectively. The results indicated that the immediate packaging of dried shelled peanuts at safe moisture level in plastic films with water vapor transmission rated of 1 g/m2/24 hr or lower is recommended. This packaging will delay critical increases in moisture content, fungal population, aflatoxin and free fatty acid contents of peanut kernels at ambient storage conditions. Keywords: Peanuts / bag types / fungal population / aflatoxin content / free fatty acid content.
ASSESSMENT OF SEEDLING ABUNDANCE, SURVIVAL AND GROWTH OF TWO DIPTEROCARP SPECIES IN PEAT SWAMP FORESTS OF BRUNEI DARUSSALAM Hazimah Haji Mohd Din; Nor Basirah Bakiri; Rahayu Sukmaria Sukri; Faizah Haji Metali
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 25 No. 2 (2018)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (597.125 KB) | DOI: 10.11598/btb.2018.25.2.805

Abstract

Dryobalanops rappa Becc. and Shorea albida Sym. are Bornean endemics of high conservation value and increasingly threatened by anthropogenic disturbances. In-situ study of seedling abundance and growth performance of these Dipterocarp species was conducted in two selected peat swamp forests of Brunei Darussalam, following a mast fruiting event in March–May 2014. Within six 6 x 6 m plots at each forest site, D. rappa seedlings at the Anduki peat swamp forest and S. albida seedlings at the Badas peat swamp forest were measured for abundance at the initial census in September 2014, as well as survival and relative growth rates (RGR) after a period of 5 months, with the final census in February 2015. We found significantly higher seedling abundance for D. rappa (1885 ± 208) than S. albida (160 ± 71). Significantly higher percentage survival was recorded for D. rappa seedlings (90.8 ± 2.2%) in comparison to S. albida seedlings (81.7 ± 2.2%). S. albida seedlings (0.24 ± 0.02 mm mm-1 month-1) showed significantly greater RGR in stem diameter than D. rappa seedlings (0.18 ± 0.02 mm mm-1 month-1), however, there were no significant differences in the RGRs based on seedling height, leaf number and biomass between D. rappa and S. albida seedlings. In terms of seedling abundance and percentage survival, D. rappa seedlings appeared to be more successful in regeneration and may potentially be used for rehabilitation of degraded tropical peat swamps and other forest types. Our results suggested that greater conservation efforts of peat swamps must be made to protect the Bornean endemic plant species, in particular S. albida.
NATURAL PRODUCTS IN ORGANIC SYNTHESIS ROBERT H. BURNELL
BIOTROPIA No. 9 (1996)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (578.989 KB) | DOI: 10.11598/btb.1996.0.9.127

Abstract

A resume of four lectures presented by the author during a one month visit to BIOTROP in 1995. The seminars were held at the University of Indonesia at Depok, the Technical Institute in Bandung, the Bogor Agricultural Institute and at BIOTROP, Bogor. The talks were grouped under the general title "The Use of Natural Products in Organic Synthesis". Key words: Organic compounds/Natural products.
Effectiveness of Dung Beetles as Bioindicators of Environmental Changes in Land-use Gradient in Sulawesi, Indonesia Shahabuddin Saleh; Uswah Hasanah; Elijonnahdi Elijonnahdi
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 21 No. 1 (2014)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (48.975 KB) | DOI: 10.11598/btb.2014.21.1.309

Abstract

Bioindicators have been widely accepted as useful tools for monitoring and detecting changes in the environment or habitat condition. By using bioindicators, it is possible to assess the impact of human activities on the biota, instead of examining the entire biota. In this paper we analyzed diversity of dung beetles (Coleoptera: Scarabaeidae) across land use gradient inCentral Sulawesi and tested the suitability of dung beetles as bioindicators for environmental changes. Ninety baited pitfall trapswere placed and several habitat parameterswere measured at five land-use types ranging from natural forest to cacao agroforestry systems to open areas in 2009 and 2012. The effectiveness of dung beetles as bioindicators of environmental changes was evaluated by the IndVal method, a method combining the specificity and fidelity of certain species with particular types of habitat or environmental conditions. Surprisingly, the results showed that the diversity of dung beetles in two types of cacao plantations were similar to the forest sites and were significantly higher than the open cultivated area. Of the 16 dung beetles species analyzed only four species could be suggested as indicator (characteristic) species while the majority of collected species were categorized as detector species. Two of them (Copris saundersi and Onthophagus forsteni) were associated with natural forest and cacao agroforestry system, thus were suggested as the indicator of shaded and cooler habitats whereas O. limbatus and O. trituber can be suggested as indicator of unshaded and warmer habitats (bare land area).Keywords: bioindicators, diversity, Scarabaeidae, habitat preferences, IndVal.
EFFECTS OF HUMIC ACID ADDITION TO FEEDS WITH HEAVY METAL CADMIUM CONTAMINATION FROM GREEN MUSSELS ON THE GROWTH PERFORMANCE OF ASIAN SEABASS Rasidi Rasidi; Dedi Jusadi; Mia Setiawati; Munti Yuhana; Muhammad Zairin Jr.; Ketut Sugama
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 26 No. 3 (2019)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (236.409 KB) | DOI: 10.11598/btb.2019.26.3.1114

Abstract

Although it contains heavy metals, the highly nutritious green mussel Perna viridis is used as a fish meal replacement in fish diet. Fortunately, humic acid (HA) has the ability to chelate heavy metals in animal feeds. Its addition in fish feed formulation is, therefore, needed to prevent the accumulation of heavy metals in the fish body. Hence, an experiment using the Completely Randomized Design (CRD) with 5 treatments and 3 replications was conducted to evaluate the performance and feed efficiency of Asian seabass Lates calcarifer juveniles on the addition of HA on its green mussel diet containing heavy metal cadmium (Cd).  Five experimental diets were formulated with different dosages of HA addition at 0, 400, 800, 1200, and 1600 mg Kg-1of feed as treatments A, B, C, D and E, respectively. The experimental diets were given to triplicate group of 15 Asian seabass juveniles with initial body weights of 4.30 ± 0.60 g in a 70-day cultivation period of feeding until satiation. The results showed that feed comsumption, feed digestibility, protein retention, growth performance and feed eficiency were significantly affected by HA addition in the fish diet. Among all treatments, HA addition of 1600 mgKg-1 produced the best biological response from the Asian Seabass. This indicated that the humic acid addition in the diet formulation can improve the utilization of feed for fish growth. The heavy metal cadmium (Cd) in the flesh of Asian seabass is still under the limit set in the food safety standard, hence, the fishes are still considered safe for human consumption. Therefore, the green mussel meal, with HA addition as an alternative source of protein, has potential as feed additive for the diet contaminated Cd of Asian seabass juveniles.
GENETIC DIVERSITY OF AMPICILLIN-RESISTANT Vibrio ISOLATED FROM VARIOUS STAGES OF TIGER SHRIMP LARVAE DEVELOPMENT WIDANARNI WIDANARNI; ANTONIUS SUWANTO
BIOTROPIA - The Southeast Asian Journal of Tropical Biology No. 15 (2000)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (219.218 KB) | DOI: 10.11598/btb.2000.0.15.160

Abstract

This research was carried  out to  study  genetic  diversity of ampicillin-resistant Vibrio  from various  stages of tiger  shrimp larvae  (Penaeus Monodon) development from,Tambak Inti Rakyat hatchery, near Labuan, West Java, Indonesia. A total of 25 ampicillin-resistant Vibrio isolates were isolated using thiosulphate  citrate bile-salt  sucrose  agar (TCBS-Agar) and seawater  complete agar (SWC-Agar). Physiological and biochemical characterization showed that the isolates could be grouped into only two species, i.e. V. harveyi from the egg stage; and V. metschnikovii from larvae and post-larval stage (i.e nauplius, zoea, mysis, PLi, PL5, PL,0, and PL,5). These isolates were also present in their respective rearing water  of  each stage  and  some natural feed.  Schizotyping analysis employing restriction endonuclease Noll (5'-GC4GGCCGC) indicated that the isolates could be grouped into  at least  13 different  genotypes.  Therefore,  schizotyping  was  more discriminative than physiological characterization. This study showed that particular groups of Vibrio colonized all stages of shrimp larvae and demonstrated closed phylogenetic relationship. These groups of Vibrio might be  the dominant microbiota which could suppress the development of other Vibrio including the pathogenic Vibrio. Key words : Shrimp/ampicillin-resistant K/fcno/schizotyping
Response of Lonkida (Nauclea orientalis L.) towards Mycorrhizal Inoculum in Waterlogged Condition Faisal Danu Tuheteru; Cecep Kusmana; Irdika Mansur; Iskandar Iskandar
BIOTROPIA - The Southeast Asian Journal of Tropical Biology Vol. 22 No. 1 (2015)
Publisher : SEAMEO BIOTROP

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (2136.803 KB) | DOI: 10.11598/btb.2015.22.1.416

Abstract

This study was carried out to examine the morpho-anatomical adaptation, survival, growth, biomass and nutrient uptake of lonkida (Nauclea orientalis L.) from different habitats inoculated with arbuscular mycorrhizal fungi on waterlogging conditions for 90 days. The results showed that the lonkida seedlings formed lenticels (100%), adventitious roots (41%) and aerenchym under waterlogging conditions. Seedling from dry land habitats, both mycorrhizal and no-mycorrhizal, have a height growth rate lower than those from other habitat types. Similarly for relative growth rates of shoot (RGRs), root (RGRr) and total (RGRt) dry weight and the ratio of root shoots. Treatments of temporal swamp habitat without waterlogging and waterlogging and savanna with waterlogging have RGRt greater than other treatments. In general, plant dry weight (root, shoot and total) and total N in roots and shoots was greater in the interactionbetween  temporalswamp habitat with no-mycorrhizal on waterlogging condition. Independently, waterlogging increase the pool of average height gain (18%), diameter (46%) and leaf area (40%), leaf length (17%) and leaf width (21%). Nonetheless, treatment waterlogging decreased variable number of leaves (9%) andAMF colonization (71%). The results of this study indicate that the seedlings from the temporal swamp has good growth and high biomass in the conditions without or with waterlogging.Keywords : arbuscular mycorrhizal fungi, lonkida, morpho-anatomical adaptations, Nauclea orientalisL, swamp, waterlogging

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