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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 12 Documents

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Search results for , issue "Vol. 8 No. 3 (2018)" : 12 Documents clear

The Effect of Morinda citrifolia L. Fruit Extract Supplemented in to Diluent Tris-Egg Yolk Toward the Abnormality of Goat's Spermatozoa after Freezing at -80°C Apriani Herni Rophi; Sri Rahayu; Gatot Ciptadi
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

The aim of this research is to know the effect of diluent Tris-egg yolk medium added with Morinda citrifolia L. extract towards the sperms abnormality after storage at -80°C for 24 hours. The extracts added in diluent Tris-egg yolk medium were 0% (P0), 10% (P1), 20% (P2), and 30% (P3). The result showed that the percentage of sperms abnormality in P0, P1, P2, and P3 were respectively 8.67%, 6.67%, 5.67%, and 10%. P2 was the lowest percentage, while P3 was the highest percentage of sperms abnormality. In addition, this research found twelve kinds of sperms abnormality divided into categories: major abnormality included the broke tail, absent head, detached head, dag defect, tapered head, proximal droplet, round head and abaxial; and minor abnormality included the coiled tail, shoe hook tail, bent tail, and distal droplet. It has been concluded that the allotment of 20% Morinda citrifolia L. extracts in Tris-egg yolk medium able to maintain the sperm morphology at -80°C storage for 24 hours. Keywords: goat, Morinda citrifolia L., post-thawing, spermatozoa.

Proximate Composition of Some Common Alternative Flour as Fish Feed Ingredient Arrum Nurjannah Herdiyanti; Happy Nursyam; Arning Wilujeng Ekawati
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Indonesia has very large territorial water, so it supports the development of the economy in the field of fisheries, one of which is through aquaculture. Recently fish meal has become the most expensive protein ingredient in aquaculture feeds. We aim to proximately analysis of some common alternative flour as fish feed ingredient i.e. fish flour, shrimp head flour, soybean flour, and bran flour to get best-recommended flour for type cultured fish. Fish flour, shrimp head flour, soybean flour, and bran flour was purchased from the fish farmer in Malang who use alternative fish feed flour for their aquaculture. Proximate analysis carried out by protein content, Moisture content, Fat and Fiber. Proximate analysis was conducted in the Fishery Products Engineering laboratory, Brawijaya University on April 2018. The results showed that shrimp head flour had higher protein content than others flour that is about 48.71%, fat and carbohydrate content about 7.99%, and 18.34% respectively. Soybean flour contains protein, fat and carbohydrate about 31.29%, 20.75%, and 30.45% respectively. Carnivorous fish culture can use high protein flour as an alternative ingredient for feed such as fish flour and shrimp head flour; balance proximate composition for omnivorous fish cultures such as soy flour and high carbo flour such as bran flour for herbivorous fish culture.Keywords: Bran flour, Shrimp head flour, Soybean flour, Proximate analysis.

Expression of Hsp70 and β-actin Genes as The Immune Response against Viral Nervous Necrosis that Infected Asian Seabass (Lates calcalifer) Yusuf Arif Wahyudi; Uun Yanuhar; Maftuch Maftuch
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

The viral nervous necrosis (VNN) is betanodavirus of the family Nodaviridae with acute infection and associated with high levels mortality up to 100% of numerous larval-stage marine and freshwater fish species. The danger signals of viral infection that are capable of activating APCs (Antigen Presenting Cells), furthermore produced molecules protein receptors such as interferon and heat shock proteins. Stimulating interferon type I (IFN I) induce several antiviral molecules, further binding the actin cytoskeleton to reach the site of infection. This study demonstrated a correlation between increased levels of Hsp70 (heat shock proteins) and actin filamentous (β-actin) within invasion wild-type isolate of ssRNA VNN from Asian Seabass (L. calcalifer) juvenile. Furthermore, expression of Hsp70 and actin as an indicator or biomarker of stressed states in fish. The reverse transcriptase polymerase chain reaction (RT-PCR) method used to finding expression Hsp70 and β-actin. Whereas nested RT-PCR used for VNN genes. The up-regulation of Hsp70 was observed on the brain tissue higher than eye tissue of sample positive infected VNN. Whereas different expression of β-actin, β-actin receptor expression tends to be stable in the organs of the brain and eye, both invaded VNN or normal, while on the eye, the ratio slightly increased based on the intensity of the band. The research shows that altered expression of heat shock protein 70 and beta-actin gene receptors in the target organs that response to invasion VNN at Asian seabass (L. calcalifer) juvenile. Alteration expression of heat shock protein 70 and beta-actin can be used as an indicator or biomarker of stress cells, especially from VNN invasion at fish. Keywords: Asian seabass, β-actin, Hsp70, Immune response, Viral nervous necrosis.

Numerical Simulation of Leslie-Gower Predator-Prey Model with Stage-Structure on Predator Rima Anissa Pratiwi; Agus Suryanto; Trisilowati Trisilowati
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

In this paper, we introduce Leslie-Gower predator-prey model with a stage-structure population on the predator. This model consists of two populations, that are prey and predator populations. Here, we divide predator into two stages. Thus, we have three classes of population in this model that are prey, juvenile predator, and mature predator. The focus of this paper is to know the interaction between the population that is affected by stage-structure in predator population in the model and to study numerically the effects of stage-structure in predator population on the interaction of prey and predator. It is found that the transition rate from juvenile to mature predator is a very important parameter which may determine the long-term behavior of both prey and predator.Keywords: Leslie-Gower model, predator-prey model, stage-structure.

Anti-hyperglycemic and Immunomodulatory Activity of a Polyherbal Composed of Sesbania grandiflora, Salacca zalacca and Acalypha indica Ahmad Zamroni; Elok Zubaidah; Muhaimin Rifa'i; Simon Bambang Widjanarko
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2018.008.03.09

Diabetes has become a serious global public health problem due to its high prevalence and mortality. Unfortunately, current anti-diabetic drugs are having some limitations and adverse effects. Therefore, searching for a new anti-diabetic agent is an urgent challenge. In this research, we examined the effectiveness of a traditional anti-diabetic polyherbal composed of Sesbania grandiflora seeds, Salacca zalacca leaves and Acalypha indica roots (2:1:1). The study was aimed to explore the anti-hyperglycemic effect of the polyherbal in STZ-induced diabetic mice and to investigate the immunomodulatory activity involved in the process of controlling hyperglycemia. Our results showed that the polyherbal water extract (150 mg.kgBW-1) could suppress blood glucose elevation and preserve pancreatic islet of diabetic mice. Moreover, the polyherbal treatment could normalize the relative amount of activated CD4+CD62L- and CD8+CD62L- T cells. The polyherbal extract also stimulated the production of CD4+CD25+FoxP3+ regulatory T cells (Tregs) which is known to play an important role in diabetes control. In addition, polyherbal treatment also increased the relative amount of anti-inflammatory cytokines IL-10 and TGF-β. These results revealed that the polyherbal extract has an anti-hyperglycemic and immunomodulatory activity that may provide beneficial function in diabetes healing. Keywords: Antidiabetes, Immunomodulator, Polyherbal, Regulatory T cell.

Comparing Vector-host and SEIR models for Zika Virus Transmission Puji Andayani; Rizal Dian Azmi; Lisa Risfana Sari
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Some mathematical models to describe Zika virus transmissions have been analyzed. In this study, we construct two models of Zika virus transmission. The first one is the vector-host model. It considers the human population as host and mosquito's population as the vector. The second model is where there is only infected human population who act as transmitter without the existence of infected mosquitoes in the population. The impact of modeling assumption of Zika virus is studied by analyzed the reproduction number using Next Generation Matrix (NGM) method. Formerly, we compare the dynamics of the two models by interpreting the reproduction number of each model. Biologically, the two models cause a similar effect. If the reproduction number is less than one, then the disease is extinct. Otherwise, an endemic condition exists. The numerical simulation also used to explain the comparison of two models. The recovery and the transmission period are solved to compare these two cases. Keywords: comparison, mathematical model, reproduction number, SEIR, Zika Virus.

Immunostimulant Activity of Neem Leaf (A. indica A. Juss) Ethanol Fraction on Tilapia (Oreochromis niloticus) Rika Putri; Sri Andayani; Ating Yuniarti
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Intensive cultivation of Tilapia with high stock density and continuous artificial feeding will decrease the water quality and increase the growth of pathogenic bacteria. That situation led to the fish stressed so that the fish become the target of bacterial pathogen attacks. Alternatively, the environmental friendly treatment using herbal immunostimulant to solve that problem is required. The herbal in this study used neem leaf (Azadirachta indica A. Juss). The aim of this study was to evaluate ethanol fraction of neem leaf on immunostimulant activity of Oreochromis niloticus. In this study, the extraction method used maceration. Fractionation methods were using Thin Layer Chromatography (TLC) and Column Chromatography (CC). The biological component was analyzed using Phytochemical, FTIR and GC-MS methods. TLC test showed that ethyl acetate: n-hexane (1:1, v/v) result in brightest luminescence colors on the plates of silica gel under UV light (365 nm). The chromatography column from the ethanol extract of neem leaf (A. indica A. Juss) showed that separation of the fraction was done based on the formation of color, which obtained 5 (five) types of fractions. The results of the phytochemical test for the fraction 4 of neem leaf contains two compounds, i.e. flavonoids (+) and triterpenoids (++). The FTIR test showed that fraction 4 of neem leaves contain a class of terpenoid and flavonoid compounds. GCMS test results on fraction 4 of the neem leaves (A. indica A. Juss) obtained the high peak, which has 4 terpenoid compounds, 1 compound of flavonoid, and 1 type of lauric acid. The total leukocyte of the fish test increased after treated with fraction 4 of neem leaf (A. indica A. Juss) compared with control on day 7 after injection, i.e. 25.29 x 104 cells.mL-1.Keywords: fraction, leukocyte, Neem Leaves, Tilapia, Phytochemicals.

White Spot Syndrome Virus (WSSV) Detection at Traditional Ponds of Lithopenaeus vannamei in Pasuruan District Ali Usman; Sri Andayani; Endang Yuli Herawati
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Vanamei shrimp (Litopenaeus vannamei) is a type of shrimp that widely cultured in Indonesia. Pasuruan is one of the districts where there are many ponds that culture shrimp Vaname (L. vannamei) traditionally. The occurrence of a decrease in production due to WSSV virus attacks that cause a lot of losses for farmers so it needs a preventive effort by doing early warning and monitoring on the existence of the virus. The study was conducted from April to May 2018 with the aim of obtaining data on the presence of WSSV virus and its prevalence at traditional Vaname shrimp farms (L. vannamei) in Pasuruan District. The sampling location is located in Pasuruan District consisting of three locations namely Bangil, Kraton, and Rejoso with each has 10 ponds (total of 30 ponds site). Detection of White Spot Syndrome Virus using Nested PCR with shrimp section taken is swimming foot, road leg, and tail. PCR results in 848 bp and 333 bp indicated the presence of WSSV infection in the Bangil and Rejoso ponds where the prevalence rate of WSSV attack in each region differs from Bangil 0 - 15%, Kraton 0% and Rejoso 0 - 15%.Keywords: PCR, Prevalence, Shrimp, Vannamei, WSSV.

Screening of Rhizosphere Bacteria from Clove (Syzygium aromaticum) in Tidore Island as Plant Growth Promoting Rhizobacteria Ismat Ishak; Tri Ardyati; Luqman Qurata Aini
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Tidore Island in North Maluku Province is one of the clove (Syzygium aromaticum) producing regions. Clove plant fertility is maintained even though it is not given organic and inorganic fertilizers, it also keeps the fertility of the soil and the environment as a whole. Therefore, this study aims to explore bacteria in the rhizosphere of the clove trees planted in Tidore island as biofertilizer agents of Indol-3-Acetic Acid (IAA) production, phosphate solubilization, and ammonium production. Bacteria were isolated from the rhizosphere of clove trees. Screening of the isolates was done according to qualitative and quantitative methods. Data collection of the qualitative method were based on medium color changes for each variable while that the quantitative method (i.e. the concentrations of produced IAA, solubilized phosphate, and produced ammonium) were obtained based on spectrophotometry. The results obtained 110 bacterial isolates. Nineteen bacteria were potential as biofertilizer agents, of which isolate R11, R8P, and A1N showed the highest in IAA production (8.71 μgL-1), phosphate solubilization (8.37 μgL-1), and ammonium production (11.71 μgL-1), respectively. The three isolates respectively have similarities to the genus of Enterobacter, Burkholderia, and Stenotrophomonas. Keywords: Biofertilizer, Clove, Rhizobacteria, Rhizosphere.

Isolation and Identification of Antagonistic Bacterium against a Pathogens of Bacterial Tuber Rot of Amorphophallus muelleri Nurfitri Arfani; Rodiyati Azrianingsih; Suharjono Suharjono
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Rhizosphere bacteria have the ability to protect the host plants from the infection of pathogenic microorganisms. This study aimed to identify rhizosphere bacteria that were capable of inhibiting the growth of bacterial isolates that cause tuber rot of Amorphophallus muelleri. Rhizosphere bacteria were isolated using Nutrient Agar medium by pour plate method. Isolates were subjected to antagonistic assay against several bacterial isolates from the rotten tuber of A. muelleri using dual culture method. The potential isolate was identified based on 16S rDNA sequence. Isolate R7 showed the strongest inhibition to the growth of bacterial isolates from rotten tuber with an inhibition zone diameter of 19.66 mm. The 16S rDNA sequence of isolate R7 R7 was 99.7% similar to Delftia tsuruhatensis PCL1755. The isolate was potential to be developed as phytopathogen control agent.Keywords: Amorphophallus, antagonistic bacteria, rhizosphere bacteria, rotten tuber, 16S rDNA.

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Jehan Ramdani Hariyati

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Home Page

OAI Link

Editorial Team

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA