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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

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Lethal Concentration 50 (LC50 - 96 hours) Nile Tilapia (Oreochromis niloticus) exposed Cypermethrin-based Pesticide Shinta Hiflina Yuniari; Asus Maizar Suryanto Hertika; Amin Setyo Leksono
The Journal of Experimental Life Science Vol. 6 No. 2 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

In aquaculture of an irrigation, the use of cypermethrin-based pesticide can harm nile tilapia which cultured within and its impact on humans who consume the fish. Thus it is necessary to study to determine the threshold of this cypermethrin-based pesticide. Determining the thresholds was using the test of LC50 - 96 hours (Lethal Concentration 50-96 hour) which tested tilapia that dead by 50%, which can be used as a benchmark threshold for cypermethrin-based pesticide. Test of LC50 - 96 hours consist of preliminary test and advanced test. Lethal Concentration 50 (LC50 - 96 hours) of cypermethrin-based pesticide towards nile tilapia (Oreochromis niloticus) is 0.082 ppm. At these doses, tilapia death reached 50%. This concentration is expressed as a threshold usage of cypermethrin-based pesticide on the environment around the aquaculture of tilapia. Keywords: Cypermehrin-based pesticide, LC5096 hour, Nile tilapia.

Anatomical Characters of Shoot Apical Meristem (SAM) on Bulbil Porang (Amorphophallus muelleri Blume) At the End of Dormancy Period Muhammad Naufal Afifi; Nunung Harijati; Retno Mastuti
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Porang is one species member of the genus Amorphophallus. In Indonesia, Porang is cultivated extensively because it has a high economic value. There is three seeded material available for planting. One of seeded material is bulbil. Bulbil is a vegetative reproductive organ of Porang plants which is located in the branch of the leaf. Bulbil has 3 - 4 months dormancy period. The dormancy period will end with shoot growth. Morphologically, there can be found a rough structure in the form of bulges in bulbil. The bulge is spread throughout the bulbil surface and is a growing area of shoots. Shoots on bulbil will grow through the bulge and only grow on the adaxial part, contrary shoots do not grow from the abaxial part. This study aims to compare the anatomy of shoot apical meristem (SAM) in the adaxial and abaxial parts of bulbil at the end of the dormancy period, ie 100 days after harvest (DAH), 110 DAH, 120 DAH, and 130 DAH. Anatomical observations were carried out by making semi-permanent preparations. The results showed that the anatomy of the adaxial and abaxial parts of bulbil Porang had differences in the development of SAM, leaf primordia, primary thickening meristem (PTM) and procambium.Keywords: Anatomy, Bulbil Porang, Dormancy, Shoot Apical Meristem.

Efek Suplemen L-Arginin Subakut Peroral pada Kontraksi Aorta Tikus Diabetes Sjarif Ismail; M. Mulyohadi Ali; Djoko W. Soeatmadji
The Journal of Experimental Life Science Vol. 3 No. 2 (2013)
Publisher : Postgraduate School, Universitas Brawijaya

Penelitian ini bertujuan untuk mengetahui pengaruh suplemen L-arginin subakut peroral pada tikus diabetes-streptozotosin terhadap respons kontraksi aorta melalui mekanisme pencegahan peningkatan stress oksidatif. L-arginine diberikan selama 8 minggu pada tikus diabetes dengan dosis 10, 100 dan 1000 mg.kg-1 BB.hari-1. Parameter yang diukur adalah MDA-plasma untuk menilai oksidatif stress dan teknik bioassay dengan isolasi organ terpisah aorta ring untuk menilai respons reseptor adrenergik-a1 di otot polos aorta terhadap fenilefrin (PE). Dari respons kontraksi aorta dapat diketahui nilai Emaks dan pD2 PE. Hasil penelitian menunjukkan bahwa pemberian L-arginin 100, 1000 mg.kg-1 BB.hari-1 pada tikus diabetes dapat mencegah peningkatan MDA-plasma (p<0.001). Pemberian L-arginin dosis 100, 1000 mg.kg-1 BB.hari-1 dapat mencegah peningkatan respons kontraksi aorta terhadap PE melalui pencegahan peningkatan Emaks (p<0.000) dan menurunkan pD2 pada dosis 1000 mg.kg-1 BB.hari-1 (p<0.001). Hasil Jalur Hubungan menunjukkan pencegahan peningkatan Emaks melalui jalur pencegahan peningkatan MDA (p<0.012) dan penurunan pD2 melalui jalur langsung (p<0.016). Berdasarkan haasil penelitian dapat disimpulkan bahwa pemberian suplemen L-arginin pada tikus diabetes dapat mencegah peningkatan respons kontraksi aorta terhadap PE dengan cara: (1) mencegah peningkatan Emaks melalui pencegahan peningkatan MDA (jalur tidak langsung); dan (2) secara langsung menurunkan afinitas reseptor adrenergik-a1.Kata kunci: diabetes, L-arginin, reseptor adrenergik-a1, stress oksidatif

Physical Properties of Turpentine and Gum Rosin Pinus merkusii Jungh et de Vriese Tapped Oleoresin by Borehole Method Agus Sukarno
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

This study was conducted to determine the physical properties of turpentine and gum rosin derived from oleoresin tapped with borehole method. The research material is derived oleoresin from Aceh provenance pine and Jember landrace. The test results of the physical properties of turpentine are as follows; color: clear, specific gravity 0.84-0.85, refraction index 1.46, negative fatty oil, flash point 34-35.5°C, residual evaporation 1.2-1.6%, and optical round + 32.2-32.4o. Result of test of physical properties of gum rosin: color X (main), softening point 76.0-76.5°C, impurity 0.0014-0.0051%, acid value 179.5-188.5, saponification value 188.7-194.3, iodine number 9.6-9.8, ash content 0.001-0.005% and volatile turpentine content 2.1-2.4%. Keywords: Aceh provenance pine, borehole method, gum rosin, turpentine.

Aktivitas Antibakteri Ekstrak Teripang Holothuria sp Terhadap Bakteri Vibrio harveyi Secara In vitro Siti Roihanah; Sukoso Sukoso; S. Andayani
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Bakteri adalah salah satu penyebab penyakit pada mahluk hidup yang dapat mematikan. Salah satu alternatif yang dapat dilakukan untuk mengatasi masalah tersebut adalah dengan menggunakan teripang (Holothuria sp) yang memiliki kandungan bioaktif sebagai bahan antibakteri. Tujuan penelitian ini adalah untuk mengetahui aktifitas antibakteri ekstrak teripang (Holothuria sp.) terhadap Vibrio harveyi, mempelajari karakter dan struktur bakteri yang dihambat oleh ekstrak bioaktif teripang (Holothuria sp.) dan mempelajari komponen bioaktif yang terkandung dalam teripang. Hasil uji cakram menunjukkan rerata zona hambat sebesar 8,502 mm sedangkan hasil uji MIC dan MBC menunjukkan bahwa pada dosis 0,55 mg/ml mampu menghambat (Bakteriostatik) dan dosis 0,60 mg/ml mampu membunuh (bakterisidal).Ekstrak kasar teripang (Holothuria sp) berpengaruh terhadap karakter dan struktur bakteri Vibrio harveyi yaitu dengan merusak dinding sel dan membran sel bakteri. Hasil analisis Spektofometri Infra Merah dari eksktrak teripang (Holothuria sp) dan analisis Spektrofotometri Ultra Violet dengan pelarut n-heksan serapan diduga mengandung senyawa Triterpenoid. Kata kunci : Aktivitas Antibakteri, Teripang Holothuria sp , Vibrio harveyi

Molecular Characterization of a Rigid Rod-Shaped Virus Isolated from Frangipani (Plumeria sp.) Showing Mosaic Symptom in Taiwan Fery Abdul Choliq; Tsang-Hai Chen; Liliek Sulistyowati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Frangipani is an important succulent plant around the worlds and also in Taiwan, for example, Plumeria rubra is widely grown as a popular ornamental tree in parks and landscaped establishments in Taiwan. Recently, a new disease in frangipani with mosaic and distortion symptoms was found in Taiwan. No viruses caused frangipani disease has been reported in Taiwan and the references about frangipani disease are still limited and only Frangipani mosaic virus (FrMV) was found. In this study, the molecular properties of a virus isolated from symptomatic frangipani in south Taiwan, such as Pingtung, Kauhsiung and Tainan were investigated. The virus with rod-shaped particles of 300 nm long and 18 nm in diameter was examined inside diseased leaves by electron microscopy. The purified virus particles showed the typical UV spectrum of tobamoviruses with A260/A280 value of 1.29 and maximum and minimum absorption at 260 nm and 249 nm, respectively. The molecular weight of 19.5 kDa as the size of coat protein of tobamoviruses was estimated by sodium dedocyl sulfate-polyacrylamide gel (SDS-PAGE). Furthermore, the degenerate primers for tobamoviruses were used to amplify 568 bp and 400 bp of the DNA fragments in RT-PCR and nested PCR, respectively. Based on these results, it was confirmed that the rigid rod-shaped virus isolated from mosaic symptom of frangipani leaves is an isolate of FrMV, belonging to the genus Tobamovirus. This is the first report thatFrMV infecting Plumeria sp. in Taiwan.Keywords: Frangipani plant, FrMV, mosaic disease, Tobamovirus.

Effect of Place and Time Storage on the Quality of Tubers Amorphophallus muelleri Blume Putri Rabiah Al Adawiah; Rodiyati Azrianingsih; Retno Mastuti
The Journal of Experimental Life Science Vol. 9 No. 1 (2019)
Publisher : Postgraduate School, Universitas Brawijaya

Porang tubers are plant commodities that physiologically susceptible to damage. Longtime storage can decrease the quality of tubers. Furthermore, the presence of fungi or bacteria makes tubers cannot be stored in a long time. Decreasing quality of Porang tuber became a problem because of the higher demand for porang tubers. This research was conducted to determine the suitable place and time storage of porang tubers. Therefore, farmers can control or reduce the decrease in tuber quality. Methods of this research were an observation of color change based on the Munsell Color System, odor observation, hardness observation using penetrometer, the rotten area of tuber was measured using Leaf Area Meter, shrinkage of tuber weight, the percentage of rotten and sprouted tubers, density, and moisture content by the oven method. The results of this study were analysed statistically by ANOVA test. The hardness of the tubers has a significant effect on the storage place (soil, rack, and floor) for period 14 weeks. Storage time has an effect on changes of the weight loss, moisture content and decay area. The longer the tuber stored the higher percentage of sprouted and rotten tubers. The color of porang tubers change and the odor in the part of the rotten tubers increase during the storage period in each storage place. Porang tubers should not be stored for more than two weeks and placed on a rack to minimize the damage of the tubers.Keywords: Physical change, porang tubers, storage time.

Minimizing Antioxidant Damage of Purple Sweet Potato var Antin 3 in Vacuum Packaging Abd. Azis Hasan; Nur Hidayat; Sri Kumalaningsih
The Journal of Experimental Life Science Vol. 5 No. 1 (2015)
Publisher : Postgraduate School, Universitas Brawijaya

The aim of this research is to find out the best immersion time in liquid nitrogen and the thickness of the packaging plastic to minimize the antioxidant damage of purple sweet potato during storage process. This research is conducted by using a factorial experiment in a randomized block design with two factors. In this research, first factor is the immersion time (0; 50; 60; 70 seconds), the second factor is the thickness of plastic (0.07 and 0.10 mm). The result of this research showed that the best immersion time is 70 seconds, while the best plastic thickness is 0.1 mm. The interaction of these two factors is the most effective to prevent antioxidants damage of purple sweet potato chips during 1 month storage.Keywords: antin 3, antioxidants, multilayer, vacuum packaging

Geographically and Temporally Weighted Regression Modeling in Analyzing Factors Affecting the Spread of Dengue Fever in Malang Fahmi Indrayani; Henny Pramoedyo; Atiek Iriany
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Geographically and Temporally weighted regression (GTWR) modeling has been developed to evaluate spatial heterogeneity and temporal heterogeneity in factors influencing the spread of dengue fever in Malang city. By using the monthly data in 2012-2015 as the temporal unit of each urban village in Malang and village is considered as a spatial unit. GTWR model is compared with the GWR model using several statistical criteria. GTWR model shows that the relationship between dengue incidence with population density and monthly average temperature significantly affects each Village in Malang.Keywords : DHF, GTWR, Spatiotemporal Pattern

Immunomodulator Testing on Ethanol Extract of Gynura procumbens Leaves to Mus musculus Adaptive Immune System: in Vitro Study Dinia Rizqi Dwijayanti; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 4 No. 1 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

Immunomodulator is a substance that has an ability to modulate the activity and function of immune system. Gynura procumbens supposed to has benefit as an immunomodulator because of it afficacy to cure many diseases. The aim of this study is to determine the effect and dose variations of G. procumbens extract on biological aspect of CD4+CD62L-, CD4+CD62L+, CD8+CD62L- and CD8+CD62L+ T cells. G. procumbens extract concentrations that used in this experiment were 0 µg /ml, 0.1 µg/ml, 1 µg/ml, and 10 µg/ml. Spleen cells were cultured for 4 days in 5% CO2 incubator at a temperature of 37°C. Cultured cells were harvested and analyzed by flowcytometry to asses cell surface molecule expression. The resulting data were tabulated and analyzed using ANOVA analysis with a significance of 0.05% on SPSS version 16. Results showed that the extract of G. procumbens can increase the proliferation of CD4+CD62L-, CD4+CD62L+, CD8+CD62L- and CD8+CD62L+ T cells compared to the control. Dose of 1 µg/ml showed the highest effect to promote cell activation compared with the dose of 0.1 µg/ml and 10 µg/ml. Dose of 10 µg/ml could suppress CD4+CD62L-, CD4+CD62L+, CD8+CD62L- and CD8+CD62L+ T cells development. This study suggests that the ethanol extract of G. procumbens has benefit as an immunomodulator and involved in the immune system. Keywords: Gynura procumbens, immunity, immunomodulator, in vitro, lymphocytes.

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Home Page

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

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Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA