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The Journal of Experimental Life Sciences (JELS)

jels
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Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 309 Documents

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A Solid Waste Pond Tiger Shrimp (Peneaus monodon) as Fertilizer for Caulerpa lentillifera Saputra, Nyoman Robby Manik; Sukoso, Sukoso; Kartikaningsih, Hartati
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Graduate School, Universitas Brawijaya

Farming the shrimp, fish and another commodity could produce large quantities of waste. Aquaculture waste can be formed as feces, residual feed and dead organism which are accumulated in a cultivation area. Generally, the waste is discharged directly into the water without filtration. Thus, one of the problems that are often faced by farmer is the low quality of pure water due to the high content of nutrients. Moreover, suspended solid pollutant will be formed that lead to eutrophication, oxygen depletion, and precipitation. The aim of this research is to analyze the solid waste pond tiger shrimp as fertilizer for the growth of Caulerpa lentiilifera. Here, the Random Design complete (RAL) and three times in Deuteronomy are used in the experiment. The parameters of one control and three treatment doses are used 0, 2, 4, and 6 g.L-1 respectively. The results showed that solid waste shrimp ponds can be used as fertilizer to meet the needs of Caulerpa lentillifera for growth and the results show the highest value is found in the dose of 6 g.L-1 with NO3- (4.58 ppm), NH4+ (3.34 ppm), PO43- (2.03 ppm) and the value of the rate of growth and the PH are obtained (3.64 g.day-1) and (6,4-8), respectively. Keywords: Caulerpa lentillifera, growth rate, Nitrification, solid waste, Tiger Shrimp.

The Effect of Biogas Waste Doses (Sludge) of Cow Manure on the Growth and the Yield of Eggplant (Solanum Melongena L.) Mas'udi, Ach Fauzan; Setyawan, Hidayat Bambang
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

The growth and the yield of eggplant plants are strongly influenced by the condition of the soil, and any damage to soil quality can cause unproductive plants according to its potential results. The addition of organic material in the form of biogas waste into the soil is expected to increase the yield of eggplant. This experiment aims at determining the best dose of biogas waste (sludge) to increase eggplant production. The experiment was carried out in Jember Lor village, Patrang, Jember, East Java with an altitude of Â± 89 meters above sea level (masl). The experiment used a completely randomized design method (CRD) with 4 treatments, treatment L0: 0kg waste plant-1; L1: 0.6 kg of waste. L2: 1,2kg of waste plants -1 and L3: 2,2 kg of waste plants-1. Each treatment was repeated 6 times with a total of 24 plants. Based on the results of the study, there was a very significant effect of sludge on variables of plant height, flowering age, harvesting age, fruit diameter, fruit length, and fruit weight. Sludge does not show a significant difference in plant diameter variables. The best treatment to have reached the plant height, fruiting age, harvesting age, fruit diameter, fruit length and fruit weight was the treatment of using a sludge with the dose of 1.2 kg polybag-1 (L2) with a height of 102.8 cm, different ages of 30.83 HST , harvest age of 56.73 HST, fruit diameter of 35.93 mm, fruit length of 17.95 cm and fruit weight of crop reached 1.36 kg (37.61 tons ha-1).Keywords: biogas waste, cow dung, eggplant, sludge.

Bioactivity of Combination Elephantopus scaber and Sauropus androgynus on the Level of B220 cells of Lymph Node in Pregnant Typhoid BALB/c Mice Nurmamulyosari, Lulut Dwi; Djati, Muhammad Sasmito; Widyarti, Sri
The Journal of Experimental Life Science Vol. 5 No. 2 (2015)
Publisher : Graduate School, Universitas Brawijaya

Elephantopus scaber and Sauropus androgynus are herbal remedies that contain flavonoids as immunomodulatory agents. This study was aimed to observe the changes in the relative number of B cells in lymph node tissue of pregnant typhoid mice. Mice were divided into seven groups K-, K+, E. scaber 200 mg.kg-1 BW, E. scaber 150 mg.kg-1 BW + S. androgynus 37.5 mg.kg-1 BW, E. scaber 100 mg.kg-1 BW + S. androgynus 75 mg.kg-1 BW, E. scaber 50 mg.kg-1 BW + S. androgynus 112.5 mg.kg-1 BW, dan S. androgynus 150 mg.kg-1 BW. Mice were dissected on the 12th and 18th day after herbal treatment. Data was analyzed using one way ANOVA (p < 0.05) and Duncan. The result show that E. scaber combination of 200 mg.kg-1 BW and E. scaber 150 mg.kg-1 BW + S. androgynus 37.5 mg.kg-1 BW can increase the number of B220 cells (p < 0,05) on pregnant mice typoid model. Keywords: B220, Elephantopus scaber, pregnant, Sauropus androgynus, typhoid fever

Heavy Metal Copper (Cu) Distribution in Water and Its Bioaccumulation in Green Mussel (Perna viridis) in Coastal Area of Ujung Pangkah, Gresik District Qodri Fitra, Mohammad; Arfiati, Diana; Semedi, Bambang
The Journal of Experimental Life Science Vol. 8 No. 2 (2018)
Publisher : Graduate School, Universitas Brawijaya

Environmental pollution in the form of increased concentration of heavy metal Copper (Cu) becomes the main factor that can affect water quality and inhibit green mussel culture (Perna viridis) in Ujung Pangkah Coastal Area of Gresik District. The purpose of this research is to analyze concentration of heavy metal Copper (Cu) on water and its bioaccumulation in green mussel (P. viridis) in Ujung Pangkah waters, Gresik District. This research will be conducted from June to August 2017. The method used in this research is descriptive method. Sampling of seawater and mussel by purposive sampling. Cu heavy metal content in Ujung Pangkah waters, Gresik District has 0.003 - 0.006 ppm in July and 0.002 - 0.006 ppm in August. That value is still safe below predetermined quality standards. The results of heavy metal measurement test in shellfish has 0.159 to 0.69 ppm in July and 0.162 - 0.71 ppm in August. The high concentrations of Cu heavy metals in Ujung Pangkah waters are simultaneous to the bioaccumulation concentration in the green mussel. The higher concentration in the waters, the higher the bioaccumulation in the green mussels. And vice versa. The distribution of Cu heavy metal concentration at point 4 is the highest. While at point 1 has the lowest Cu heavy metal concentration. Keywords: Copper, Bioaccumulation, Green Mussel, Heavy Metal, Perna viridis.

Penampilan Kandungan Protein Dan Kadar Lemak Susu Pada Sapi Perah Mastitis Friesian Holstein Suryowardojo, Puguh
The Journal of Experimental Life Science Vol. 2 No. 1 (2012)
Publisher : Graduate School, Universitas Brawijaya

Penelitian ini dilaksanakan di peternakan wilayah kerja Proyek Peternakan Sapi Perah GKSI Jawa Timur di Desa Sawiran, Kecamatan Purwodadi, Kabupaten Pasuruan dan di Laboratorium Ternak Perah Fakultas Perternakan Universitas Brawijaya. Tujuan penelitian ini adalah untuk mengetahui penampilan kadar protein dan kadar lemak susu pada tingkat mastitis yang berbeda pada sapi perah. Hasil penelitian ini diharapkan dapat dimanfaatkan sebagai informasi tentang pentingnya melakukan pencegahan penyakit mastitis sebagai salah satu faktor penyebab perubahan kadar protein dan kadar lemak susu yang dihasilkan. Materi penelitian adalah sampel susu dari kuartir ambing 30 ekor sapi perah (120 puting) laktasi, dengan bulan laktasi 2 sampai 3 dan tingkat laktasi 2 sampai 3. Metode penelitian yang digunakan adalah study kasus. Observasi langsung dilapang yang dilakukan untuk mengetahui tingkat mastitis pada sapi perah berdasarkan California Mastitis Test (CMT). Dan untuk menguji kadar protein dan kadar lemak dilakukan di Laboratorium Ternak Perah. Hasil penelitian menunjukkan bahwa puting sapi perah sampel berada pada tingkat nol sebanyak 5 ekor (16,67 %) dan yang terkena mastitis sebanyak 25 ekor (83,33 %). Tingkat mastitis dan protein serta lemak susu mempunyai hubungan yang linier negatif yang sangat nyata (p ≤ 0,01). Berdasarkan persamaan regresi semakin tinggi tingkat mastitis maka kadar protein dan lemak susu semakin menurun. Kesimpulan dari penelitian ini adalah semakin tinggi nilai mastitis maka kadar protein dan kadar lemak susu semakin menurun sebesar 53 % dan 33 %. Dari penelitian ini dapat disarankan agar peternak lebih menjaga kesehatan lingkungan untuk mencegah berjangkitnya penyakit mastitis pada peternakan tersebut. Kata Kunci: Friesian Holstein, mastitis, lemak susu, protein

Phytochemical and Histochemical Screening of Toxic Plant Based on Knowledge of Tengger Tribe in Ngadiwono Village, Pasuruan Oktavia, Anggraeni In; Batoro, Jati; Indriyani, Serafinah
The Journal of Experimental Life Science Vol. 7 No. 1 (2017)
Publisher : Graduate School, Universitas Brawijaya

Around hundreds of plant's species have been confirmed to produce toxic that could endanger the life of human or domestic animals. Some of those plants have potentials to be developed as medicine. The objective of this study is to reveal the secondary metabolite content of toxic plants mentioned by Tengger tribe on Ngadiwono village through phytochemical and histochemical screening test. This study was conducted on Ngadiwono village, Tosari district, Pasuruan Regency. Informants were selected using snowball method by following the information of key person (n informant=14). Plants were selected and collected according to the local knowledge, then was analyzed qualitatively for its secondary metabolite content through phytochemical and histochemical screening test. The results showed that there are 8 plant species categorized as toxic by local people, including Bedor (Girardinia palmata), Kecubung Bunga Kuning (Brugmansia suaveolens), Kecubung Bunga Putih (Brugmansia suaveolens), Jarak (Ricinus communis), Terpasan Kuning (Cestrum elegans), Terpasan Merah (Cestrum elegans), Kudisan (Euphorbia pulcerrima), and Ciplukan (Physalis peruviana). The phytochemical result indicated that all toxic plants mentioned by the local people contain alkaloid substancess, while histochemical test showed that alkaloid substances were found in leaf trichomes, except in Terpasan Merah (Cestrum elegans). Keywords: Fitochemical, Histochemical, Tengger Tribe, Toxic Plant.

The Study of Growth and Its Polyembryonic Properties of Porang Seeds (Amorphophallus muelleri Blume) from Various Fruit Colors in Different Planting Media Wardani, Imaniah Bazlina; Harijati, Nunung; Mastuti, Retno
The Journal of Experimental Life Science Vol. 9 No. 2 (2019)
Publisher : Graduate School, Universitas Brawijaya

The ripening of Porang compound fruit (Amorphophallus muelleri Blume) in one cob does not occur simultaneously but moves forward from the distal region to proximal. The ripe fruit is red, almost ripe is yellow and unripe is green. The study aimed to examine the growth potential and polyembryonic properties of Porang seeds derived from red, yellow and green fruits. These seeds were grown in different media with the expectation the best plant growth can be obtained. This research was a factorial experiment which was arranged in a completely random design and each unit observation has three replication. The factor I was the source of seeds. Seeds came from various fruit colors. Each of them is red (R), yellow (Y) and green (G). Factor II is a planting medium which had 4 types, M1, M2, M3, and M4. M1 is combination of soil and husk charcoal (70%: 30%), M2 = soil: cocopeat (70%: 30%), M3 = soil: husk charcoal: cocopeat (70%: 15%: 15%) and M4 = just soil (100%). The results showed that the seeds derived from red fruit and planted on medium 2 (soil: cocopeat = 70%: 25%) potentially producing the best plant. The seeds also showed 100% polyembryony at 182 days after planting. In addition, the first, second and third shoots (originating from one seed) have different in height, canopy diameters, and petiole diameter. Keywords: Fruitcolors, planting media, polyembriony, Porang (Amorphophallus muelleri Blume).

Pengaruh Pemberian Kalsium terhadap Pertumbuhan Plasmodium falciparum in Vitro Asfirizal, Verry; Soebaktiningsih, Soebaktiningsih; Sudjari, Sudjari; Sumarno, Sumarno; Fitri, Loeki Enggar
The Journal of Experimental Life Science Vol. 1 No. 1 (2011)
Publisher : Graduate School, Universitas Brawijaya

Peningkatan permeabilitas sel eritrosit yang terinfeksi Plasmodium falciparum terhadap ion dan makromolekul diketahui sebagai mekanisme parasit untuk memenuhi nutrisi dalam proses pertumbuhan. Peningkatan permeabilitas terhadap kalsium masih merupakan hal yang kontradiktif dalam peranannya meningkatkan pertumbuhan Plasmodium falciparum dalam sel eritrosit. Tujuan dari penelitian ini adalah mengetahui peningkatan pertumbuhan Plasmodium falciparum dalam sel eritrosit pasca pemberian kalsium. Biakan primer Plasmodium falciparum dalam medium biakan RPMI 1640 yang menghasilkan parasitemia 15%, dilakukan inokulasi untuk pembuatan subkultur yang menghasilkan parasitemia 2% dan dilakukan pembagian untuk kelompok perlakuan pemberian kalsium dan kontrol (ML 10%) dengan replikasi 3 kali. Pengamatan dilakukan hari pertama sampai hari ke-6 setelah perlakuan. Pengamatan pertumbuhan dilakukan dengan parameter parasitemia, bentuk skizon, hemolisis dan kalsium intraseluler. Hasil penelitian menunjukkan bahwa pemberian kalsium menghasilkan peningkatan tertinggi jumlah total rerata parasitemia (11,09 ± 4,01) (Rerata ± SD), bentuk skizon (23,52 ± 10,83), hemolisis (0,278 ± 0,03) dan kalsium intraseluler (6,55 ± 1,88), dibandingkan dengan media biakan kontrol (ML 10%). Analisis T-test (α= 0,05) menghasilkan perbedaan yang signifikan pada parameter parasitemia, bentuk skizon, hemolisis tetapi tidak memberikan perberbedaan yang signifikan pada parameter kalsium intraseluler. Kata kunci: glukosa, hemolisis, kalsium, kalsium intraseluler, parasitemia, skizon

The Immunomodulatory Effect of Elephantopus scaber and Sauropus androgynus Extract to Cellular Immune Response in Pregnant Mus muscullus Infected by Salmonella typhimurium Jannah, Nur; Djati, Moch. Sasmito; Widyarti, Sri
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Graduate School, Universitas Brawijaya

Pregnancy has a high risk and is more susceptible to infectious diseases. E. scaber and S. androgynus contains saponins and flavonoids as an immunomodulatory that can increase the body's immunity. The purpose of this study is to determine the immunomodulatory effects of E. scaber and S. androgynus leaf extract against cellular immune response in pregnant mice infected S. typhimurium. This research used seven treatment groups including negative control (K-) mice without injected S. typhimurium, positive control (K+) mice were injected S. typhimurium and extract treatment with 5 variant doses and dissected on the 12th and 18th days. Lymphocytes was isolated from the blood, then relative number of CD4+ and CD8+ were analyzed with flow cytometry and data continued with ANOVA. Statistical analysis showed that different extract dose and duration of treatment had a significant effect on the number of CD4+ and CD8+ T cells. E. scaber and S. androgynus act as immunomudulatory effect and treatment with combination of extracts E. scaber and S. androgynus 50 mg.kg-1 BW + 112.5 mg.kg-1 BW respectively and treatment with extract of S. androgynus 150 mg.kg-1 BW are the optimum treatment which can restore immune system conditions such as normal pregnancy without infection.Keywords: Cellular Immune, E. scaber, Immunomodulatory, S. androgynus, S. typhimurium.

The Abnormality of Spermatozoa Goat after Freezing on -80°C Using Tris Diluent Added Combination Hatching Egg Yolk and Amnion Fluid Nisa, Liza Choirun; Rahayu, Sri; Ciptadi, Gatot
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Graduate School, Universitas Brawijaya

This study aims to determine the combination of the hatching egg yolk (HEY) and amniotic fluid (AF) in Tris diluent to the type of goat spermatozoa abnormalities after frozen at temperature -80°C. This study used four treatments and 6 replications. The semen is diluted using tris extender and added treatment, there are control group (T0) (Tris egg yolk 20%), T1 (15% HEY + 5% AF), T2 (10% HEY + 10% AF), and T3 (5% HEY + 15% AF). After dilution, the semen was equilibrated for 2 hours, then stored using Mr. Frosty and frozen at temperature -80°C in the ultra-freezer for 24 hours. The type of spermatozoa abnormalities type was evaluated on fresh semen and after thawing. The data were analyzed using ANOVA (P<0.05). The results showed that there is no significant difference among treatments. The primary abnormalities in fresh semen, there were macro-cephalic, amorphous, and the secondary spermatozoa abnormalities such as detached head, mid-piece defect, coiled tail, loosehead, shoe-hook tail, and a broken tail. The secondary abnormalities in post-thawing were found both in the control group and treatment, which are detached head, loosehead, coiled tail, shoe-hook tail, and broken tail. The conclusion of this study was the percentage of secondary abnormalities after thawing was increasing both in the control and treatment groups. Keywords: Amniotic Fluid, Hatching Egg Yolk, Mr. Frosty, Type of Spermatozoa Abnormality.

Page 15 of 31 | Total Record : 309

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Home Page

OAI Link

Editorial Team

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Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

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Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA