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The Journal of Experimental Life Sciences (JELS)
Published by Universitas Brawijaya
ISSN : 20872852     EISSN : 23381655     DOI : 10.21776/ub.jels
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience
The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.
Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)
Articles 299 Documents
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The The Effect of Gamma Irradiation on In Vitro Explant Growth, Shoot Multiplication, and Plantlet Regeneration Explants Bulbil of Porang (Amorphophallus muelleri Blume) Ni'amul Albab, Muhammad; Widoretno, Wahyu; Siswanto, Dian; Waluyo, Budi; Mertasari, Chaerani
The Journal of Experimental Life Science Vol. 15 No. 1 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.01.06

Abstract

Porang (Amorphophallus muelleri Blume) is a tuberous plant that contains glucomannan as an alternative in reducing obesity. It is widely traded, but it is cultivated through conventional methods and takes a long time. In vitro mutagenesis technique with gamma irradiation is expected to increase the production of superior porang. This study aims to determine the effect of gamma irradiation on explant growth, shoot multiplication, and plantlet regeneration on in vitro culture. The doses of gamma irradiation, i.e. 0, 2, 4, 6, 8, and 10 gamma rays (Gy). Explants bulbil were precultured for 14 days on Murashige and Skoog (MS) medium fortified with 6-benzyl amino purine (BAP) 3 mg.L-1 before irradiation. The irradiated explants were then cultured on media containing MS supplemented with α-naphthalene acetic acid (NAA) 0.1 mg.L-1 and BAP 3 mg.L-1 for 4 and 8 weeks of culture. Plantlet regeneration media was MS supplemented with NAA 0.1 mg.L-1 for 4 weeks of culture. The results showed that a dose of 10 Gy gamma irradiation was able to inhibit explant growth (0.06 g), shoot multiplication (0%), and plantlet height (0.38 cm), while gamma irradiation at a dose of 6 Gy increased explant growth (0.16 g), shoot multiplication (40%), and plantlet height (5.60 cm). The lethal dose 50 (LD50) of gamma irradiation for explant survival rate is 8.79 Gy. Keywords: Amorphophallus muelleri, explant growth, gamma irradiation, in vitro, plantlet regeneration, shoot multiplication
Histopathologic Evaluation of Gastrointestinal Endoscopic Biopsies with Risk Factor Analysis in a Tertiary Care Hospital in South India Lalamiya Abdul Rahiman, Baba; Maruthamuthu , Rajendran; Padipurakkal Haneefa, Shajitha; Kalimuthu, Senthilkumar
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.02

Abstract

Gastrointestinal tract (GIT) disorders are a major common problem with morbidity and mortality.  The disorders can cause inflammatory and neoplastic changes in the GIT.  Endoscopy and biopsy, in combination, play a central role in the early diagnosis and management of the disease.  This research study carried out the histopathological evaluation of GIT endoscopic biopsies and risk factor analysis. A total of 171 endoscopic biopsies were examined in this prospective study. The received formalin-fixed tissues were processed for histopathology analysis in the department of pathology through Hematoxylin-Eosin staining, and slides were mounted using Dibutylphthalate Polystyrene Xylene (DPX). Additional sections were stained with periodic acid Schiff (PAS) stain to observe adenocarcinoma. The gastric neoplastic changes incidence among patients with endoscopic biopsies sample was about 9 (5.3%), others diagnosis with Helicobacter Pylori 18 (10.5%), congestion 8 (4.7%), candida 8 (4.7%), and normal or non-neoplastic 128 (74.9%). The diagnosed malignancies were adenocarcinoma and squamous cell carcinoma. The diagnosed histopathology of the endoscopy biopsy samples was found to be mostly chronic gastritis and also neoplastic changes. Therefore, endoscopy with a biopsy of histopathology can be useful in diagnosing GIT disease. Further studies required with more cases with clinical data could be helpful for this research.  
Dewatered Sludge Utilization from Leachate Treatment Plant (LTP) for Composting at Supit Urang Landfill, Malang Susanto, Dony Tri; Kartikaningsih, Hartati; Yanuwiadi, Bagyo
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.03

Abstract

The Leachate Treatment Plant (LTP) at Supit Urang Landfill produces a by-product of dewatered sludge that has not been utilized optimally and according to its potential. Sludge is a highly organic material that agriculture can reuse as a fertilizer or soil conditioning agent. However, its potential heavy metals possess environmental risks. Currently, sludge from the dewatering process is disposed of in landfills. Sludge left open without further handling will potentially be a source of environmental pollution that will cause unpleasant odors. This study aims to identify how to utilize sludge as raw material for compost mixtures. The approach applied in this research was quantitative with an experimental design. Sludge was mixed with ready-made compost and EM4 solution. Composting is carried out with three different ratios of sludge and compost, which consist of (w/w) 1:1 (M1), 1:2 (M2), and 1:3 (M3). The addition of EM4 solution (10% of the mixture weight) was used to enhance the efficiency of the composting process. The result revealed that after 21 days, the quality of co-composting sludge and ready-made compost met the requirements of SNI 19-7030-2004. The best results were obtained in mixture M3 (C-Org = 17.92%, Ntotal = 1.71%, Ptotal = 0.80%, Ktotal = 1.33%, C/N-ratio = 10.38, Cd = 2.47 mg.kg-1, Hg = 0.0 mg.kg-1). These findings suggest that sludge has the potential to be used as raw material for compost mixtures.  
Utilization of Fly Ash as a Compost Amendment in Enhancing the Growth of Mustard Plants Aziz, Abdul; Khusaini, Mohammad; Parmawati, Rita
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.04

Abstract

Fly ash is the primary non-hazardous waste generated during the electricity production process in coal-fired steam power plants, such as thermal power stations (Pembangkit Listrik Tenaga Uap - PLTU) Paiton. Tests of fly ash characteristics have shown that it contains beneficial compounds, including silicon (Si), aluminum (Al), iron (Fe), calcium (Ca), magnesium (Mg), sodium (Na), and potassium (K), which can serve as valuable nutrients for plants. This study aims to assess the advantages of incorporating fly ash into compost as a mixture for the growth of mustard plants. Utilizing a quantitative approach, the research involved monitoring the height and leaf count of the test plants. The preparation of fly ash-based compost (FABA) was accomplished by blending fly ash with compost fertilizer in a 1:1 ratio. Five different compositions of planting media mixed with FABA compost were evaluated: K1 (0%) as the control, K2 (25%), K3 (50%), K4 (75%), and K5 (100%), with five replications for each. The results indicated that the most favorable plant height growth occurred with a mixture containing 25% compost-fly ash, yielding an average plant height of 11.45 cm and an average of 9 leaves per plant. An ANOVA test performed on the research data confirmed a significant difference attributed to the inclusion of fly ash in the compost mixture. When appropriately utilized, fly ash can provide farmers with a solution to reduce reliance on chemical fertilizers, thereby promoting more environmentally friendly agricultural practices.  
Corrigendum: Evaluating SARS-CoV-2 Spike Protein Transfection in HEK-293T Cells for VLP Applications Rohmah, Ilmiana Nurur; Marlita, Marlita; Kusuma, Kavana Hafil; Christina, Yuyun Ika; Dwijayanti, Dinia Rizqi; Mustikaningtyas, Dewi; Widodo, Nashi; Djati, M. Sasmito
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.01

Abstract

The spike protein of SARS-CoV-2 is crucial for initiating infections by binding to host cells and mediating membrane fusion. In this study, HEK-293T cells were transfected with plasmids encoding three structural proteins of SARS-CoV-2, i.e., Spike (S), Membrane (M), and Envelope (E). This transfection enabled the formation of SARS-CoV-2 Virus-Like Particles (VLPs), which allows for safer studies of the virus and its proteins. To serve as a marker for expression, an enhanced green fluorescent protein (EGFP) was fused to the spike protein, resulting in a Spike-EGFP (S-EGFP) fusion protein. The characteristics of SARS-CoV-2 spike protein expression in the transfected HEK-293T cells were then investigated using polymerase chain reaction (PCR) and flow cytometry. The PCR analysis revealed non-specific DNA band smearing, which did not provide conclusive confirmation of spike protein expression. However, flow cytometry analysis demonstrated that approximately 30% of the transfected cells exhibited green fluorescence, indicating the expression of the Spike-EGFP fusion protein. These findings, obtained through flow cytometry, confirmed the successful spike protein expression in transfected HEK-293T cells.  
Integrated and Sequential Enzyme-Assisted Extraction of Fucoidan and Alginate from Sargassum echinocarpum Sugiono, Sugiono; Hidayat, Mohammad Taufiq; Wahyuni, Endang Tri; Syafii, Syafii; Fikriyatullatifah, Anisa; Mumtazah, Lutfia Anis
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.05

Abstract

The sequential extraction biorefinery process can use brown algae as its feedstock, offering a sustainable approach to maximizing the utilization of marine biomass for high-value bioactive compounds. This method enables the recovery of multiple bioactive components from a single raw material, increasing process efficiency and reducing waste. Fucoidan and alginate, two valuable polysaccharides in brown algae, have attracted significant attention due to their diverse applications in the pharmaceutical, nutraceutical, and food industries. This research aims to develop an enzyme-assisted extraction (EAE) process that maximizes the fucoidan yield of Sargassum echinocharpum in the integrated alginate and fucoidan extraction biorefinery process. The effect of the parameter process, including temperature, time, and solvent-to-algae ratio, was evaluated to maximize fucoidan and alginate yield and determine the correct equation model in the first order of the experiment. The study showed that temperature, time, and the ratio of solvents all had a real effect on how quickly the fucoidan and alginate yields increased and then decreased after reaching their highest point. The temperature of 50°C, time of 24 hours, and the solvent-to-algae ratio of 30:1 (v/w) resulted in the highest fucoidan yield of 4.25% and alginate yield of 35.3%,  along with an intrinsic viscosity of 78.91 mL.g-1, molecular weight of 73.58 kDa, and an M/G ratio of 0.35.  Statistical analysis revealed a quadratic relationship, suggesting the presence of an optimal extraction condition. These findings highlight the potential of enzyme-assisted biorefinery techniques for improving fucoidan and alginate extraction. This extraction process demonstrates significant potential for industrial-scale application in the food, pharmaceutical, and nutraceutical industries due to its efficiency and sustainability.  
Effect of Acid-Base Activation on Extracted Tea (Camellia sinensis) Leaf Biosorbent for Hexavalent Chromium Removal Devianto, Luhur; Eunice, Samella; Haji, Alexander; Wirosoedarmo, Ruslan; Kurniati, Evi; Anugroho, Fajri
The Journal of Experimental Life Science Vol. 15 No. 2 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.02.06

Abstract

Industrial wastewater often contains heavy metals such as hexavalent chromium [Cr(VI)], a toxic and carcinogenic pollutant widely generated from activities including electroplating, leather tanning, and pigment manufacturing. Due to its high solubility and mobility in aquatic environments, Cr(VI) poses significant environmental and public health hazards, even at low concentrations. There is increasing interest in sustainable, low-cost alternatives for heavy metal removal, such as biosorption using agricultural waste materials. Tea leaves (Camellia sinensis L.), one of the most consumed beverages globally, generate a large volume of organic waste that is rich in functional groups capable of metal binding. This study investigates the potential of extracted tea leaf biomass as a biosorbent for Cr(VI) removal from aqueous solutions. The research aims to characterize the effect of acid-base activation on biosorbent by determining the optimal activation method, evaluating adsorption performance under different operational parameters, and identifying the most appropriate adsorption isotherm model. The biosorbent was prepared from ethanol-extracted green tea leaf waste (for tannin extraction that will be used for other applications) and activated using sodium hydroxide (NaOH) or sulfuric acid (H₂SO₄) at various concentrations.  Fourier Transform Infra Red analysis revealed key functional groups as hydroxyl (-OH) bond responsible for metal ion binding. Activation using 0.1 M NaOH resulted in the adsorption capacity range 1.22 – 12.43 mg.g-1. The maximum Cr(VI) removal efficiency of 99.42% was achieved at a contact time of 180 minutes and an initial metal concentration of 100 ppm. Adsorption equilibrium data closely followed the Langmuir isotherm model (R² = 0.999), indicating monolayer adsorption on a homogeneous surface. These findings demonstrate that NaOH-activated of extracted tea leaf biomass still has a potential biosorbent for Cr(VI) removal, offering an option of an eco-friendly solution for wastewater treatment applications.  
The Physicochemical Properties of Butterfly Pea Flower (Clitoria ternatea L.) Extracts: Influence of Various Extraction Conditions Sitompul, Johannes Nathanael; Martati, Erryana
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.01

Abstract

Butterfly pea flowers (Clitoria ternatea L.) have health benefits because they contain phenolic compounds, flavonoids, and anthocyanins, which act as antioxidants. There are few comparative studies on how the brewing temperature of butterfly pea flowers (hot, room, and cold temperatures) affects the release of antioxidants and anthocyanins. This study examined the effect of brewing methods on the physicochemical characteristics of butterfly pea flower extract. A nested experimental design was used, with brewing time nested within each brewing method. The brewing methods studied were hot brewing (HB) at 70°C for 5 and 15 minutes, room temperature (RT) brewing at 25-27°C for 3 and 6 hours, and cold brewing (CB) at 5-7°C for 12 and 24 hours. The color, pH, total anthocyanin content, and antioxidant activity were measured for the extract. The data obtained were statistically analyzed using ANOVA and Tukey's advanced test. The results showed that the brewing method significantly affected antioxidant activity but did not affect the total anthocyanin content, pH, or color. Brewing duration significantly influences all parameters except the color parameter b*. The best treatment of brewing methods for butterfly pea flowers was hot brewing for 15 minutes by simple additive weighting (SAW) method. The phenolic, flavonoid, alkaloid, and coumarin component groups made up the phytochemical composition of the butterfly pea flower extract.
Optimising Natural Bait Selection for Sustainable Handline Fishing: Behavioural Phase Analysis of Juvenile Barramundi (Lates calcarifer) in Response to Bait Type and Body Size Septiyani, Anita; Fuad, Fuad; Wiadnya, Dewa Gede Raka; Nurdiani, Rahmi; Hariati, Anik Martinah
The Journal of Experimental Life Science Vol. 15 No. 3 (2025)
Publisher : Graduate School, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.jels.2025.015.03.02

Abstract

Understanding the natural bait preference of predatory fish, such as barramundi, plays a crucial role in improving capture efficiency. This knowledge also supports sustainable fisheries by optimizing bait selection for handline fishing and reducing reef damage by active gear where barramundi species commonly live. This study examines the response of barramundi to various types of natural bait and investigates whether body size plays a significant role in this response. Predatory fish are divided into three different sizes (9, 13, and 15 cm total length), with each size having nine replicates. Fishing behavior and response to baits were divided into four phases: arousal, searching, finding, and uptake. Kinovea computer software (www.kinovea.org) was used to identify each phase with visual support from iVcam. The experiment was designed using a 54 L aquarium filled with saline water of 32 ppt. Data were analyzed using one-way ANOVA in SPSS version 26. When significant differences (p<0.05) were found, Tukey's post hoc test was performed. Bait from trash fish and shrimps did not show significant differences (p>0.05). Worms provided the fewest responses on times and behavior. Body size did not significantly affect behavioral responses or response times (p > 0.05). In conclusion, bait type significantly influenced barramundi's responses, with worms eliciting a significantly slower response compared to shrimp and trash fish, which were equally effective.

Page 30 of 30 | Total Record : 299

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