Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

The Journal of Experimental Life Sciences (JELS)

jels
Website

Published by Universitas Brawijaya

ISSN : 20872852 EISSN : 23381655 DOI : 10.21776/ub.jels

Core Subject : Health, Science,

Biochemistry, Genetics & Molecular Biology Immunology & microbiology Medicine & Pharmacology Neuroscience

The Journal of Experimental Life Science (JELS) is a scientific journal published by Postgraduate School, University of Brawijaya as distribution media of Indonesian researcher’s results in life science to the wider community. JELS is published in every four months. JELS published scientific papers in review, short report, and articles in Life Sciences especially biology, biotechnology, nanobiology, molecular biology, botany, microbiology, genetics, neuroscience, pharmacology, toxicology, and Applied Life Science including fermentation technology, food science, immunotherapy, proteomics and other fields related to life matter. JELS is a scientific journal that published compatible qualified articles to the academic standard, scientific and all articles reviewed by the expert in their field. The Journal of Experimental Life Science (JELS) have a vision to become qualified reference media to publish the best and original research results and become the foundation of science development through invention and innovation on cellular, molecular, nanobiology, and simulation work related to life matter rapidly to the community. The Journal of Experimental Life Science (JELS) has objectives to published qualified articles on research’s results of Indonesian researchers in life science scope. JELS encompasses articles which discuss basic principles on natural phenomenon with cellular, molecular, and nanobiology approach.

Arjuna Subject : Biokimia, Genetika dan Biologi Molekuler - Biokimia, Genetika dan Biologi Molekuler (Lain-Lain)

Articles 299 Documents

14 15 16 17 18

Pengaruh Sari Seduh Teh Hitam (Camellia sinensis) terhadap Ekspresi IGF-1, ERK1/2 dan PPARɣ pada Jalur MAPK (Mitogen Activated Protein Kinase) Jaringan Lemak Viseral Tikus Wistar dengan Diet Tinggi Lemak Hendra Susanto; M. Rasjad Indra; Setyawati Karyono
The Journal of Experimental Life Science Vol. 2 No. 2 (2012)
Publisher : Postgraduate School, Universitas Brawijaya

Prevalensi obesitas meningkat di berbagai Negara Asia Pasifik, Eropa, Amerika Serikat, Australia serta di negara-negara berkembang. Perubahan preadiposit menjadi adiposit yang mature memegang peranan dalam patogenesis obesitas. Teh hitam memiliki komponen antioksidan yang banyak berperan dalam pengontrolan proliferasi sel. Theaflavin pada teh hitam kemungkinan dapat menghambat perubahan preadiposit menjadi mature adiposit, maka prevalensi terjadinya obesitas sebagai pemicu berbagai penyakit metabolik dapat dicegah sejak dini. Tujuan penelitian ini untuk membuktikan pengaruh pemberian sari seduh teh hitam (Camellia sinensis) terhadap pencegahan peningkatan ekspresi IGF-1, ERK1/2 dan PPARɣ pada tikus wistar dengan diet tinggi lemak. Penelitian dilakukan secara in vivo dengan menggunakan tikus strain wistar. Tikus dibagi menjadi kelompok kontrol positif dan kelompok perlakuan. Kelompok kontrol positif diberi diet tinggi lemak tanpa sari seduh teh hitam, sedangkan kelompok perlakuan diberi diet tinggi lemak dan sari seduh teh hitam dengan dosis 0,015 g, 0,030 g dan 0,045g/hari selama 90 hari secara bersamaan. Ekspresi IGF-1, ERK1/2 dan PPARɣ diukur secara semikuantitatif dengan menghitung intensitas warna dengan Corell Photo Paint 11. Skor intensitas warna ekspresi IGF-1, ERK1/2 dan PPARɣ hasil pewarnaan imunohistokimia dianalisis secara statistik dengan One way Anova dan analisis regresi dengan taraf signifikansi p=0,05. Hasil penelitian menunjukkan bahwa sari seduh teh hitam dengan berbagai dosis (0,015 g, 0,030 g dan 0,045 g/hari) dapat mencegah peningkatan ekspresi IGF-1, ERK1/2 dan PPARɣ secara signifikan (p=0,000) dibandingkan dengan kelompok kontrol positif. Semakin besar dosis teh hitam maka ekspresi IGF-1, ERK1/2 dan PPARɣ makin rendah. Berdasarkan analisis regresi dengan estimasi kurva logaritmik (p<0,05) terdapat hubungan fungsional dua arah yang kuat antara ERK1/2 dengan PPARɣ (R=0,798 dan R=0,780). Hasil penelitian ini membuktikan bahwa sari seduh teh hitam (C. sinensis) mampu mencegah peningkatan ekspresi IGF-1, ERK1/2 dan PPARɣ pada tikus wistar dengan diet tinggi lemak. Kata kunci: diet tinggi lemak, IGF-1, ERK1/2, jalur MAPK, PPARɣ, teh hitam.

Assessing the Genotoxic Potentials of Methomyl-based Pesticide in Tilapia (Oreochromis niloticus) Using Micronucleus Assay R Adharyan Islamy; Uun Yanuhar; Asus Maizar Suryanto Hertika
The Journal of Experimental Life Science Vol. 7 No. 2 (2017)
Publisher : Postgraduate School, Universitas Brawijaya

Pesticides are recognized as serious pollutants in the aquatic environment with the potential to cause genotoxic on the aquatic organism, especially fish. The micronucleus (MN) assay has been used to evaluate genotoxicity of many compounds in polluted ecosystems such pesticides. The aim of this study to determine genotoxic effect of methomyl-based pesticide on tilapia (Oreochromis niloticus). Fish were exposed to six different concentrations base on range finding test (0 ppm, 3.2 ppm, 4.2 ppm, 6.5 ppm, 8.7 ppm and 10 ppm) of methomyl-based pesticide. The micronucleus were collected from peripheral blood erythrocyte of fish after 96 h exposure. Peripheral blood samples smears were stained with Giemsa, MN frequencies were counted and statistically analyzed using one-way ANOVA. The result of this study showed after 96 hours exposed to methomyl-based pesticide, at concentration 0 ppm causes 0% mortality, at concentration to 3.2 ppm causes 30% mortality, at concentration 4.2 ppm causes 60% mortality, at concentration 6.5 ppm causes 70% mortality, at concentration 8.7 ppm causes 80% mortality, at concentration 10 ppm causes 100% mortality of fish test. Lethal Concentration 50 (LC50 - 96 hours) of methomy-base pesticide towards tilapia (O. niloticus) is 4.015 ppm. Through micronuclei assay during 96 hour exposure of methomyl-based pesticide, the result shows that frequencies of micronuclei in erythrocyte of fish test at concentration at 0ppm is 12â€°, 18â€° and 16â€°; at concentration at 3.2ppm is 33â€°, 26â€° and 29â€°; at concentration at 4.2ppm is 41â€°, 38â€° and 46â€°; at concentration at 6.5ppm is 68â€°, 81â€° and 82â€°; at concentration 8.7ppm is 133â€°, 130â€° and 137â€°; at concentration 10ppm is 163â€°, 166â€° and 156â€°. It revealed that methomyl-based pesticide exposure induced after 96 h significantly (P<0.05) increased genotoxic potentials simultaneous with increased concentration.Keywords: Genotoxic, Methomyl, Micronucleus Assay, Pesticide, Tilapia.

Phytochemical Screening by FTIR Spectroscopic Analysis and Antibacterial Activity of Sesbania grandiflora Ndahawali, Suryaningsih; Andayani, Sri; Hardoko, Hardoko
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Sesbania grandiflora is a medicinal plant that has been used by many Indonesian people. They have the ability as antibacterial because they contain bioactive compounds that inhibit bacterial growth. Sesbania grandiflora also contains other active compounds such as tannins, saponins, steroids, flavonoids, and terpenoids. This study aims to determine the antibacterial activity of extracts, n-hexane fraction, ethyl acetate fraction and water fraction of Sesbania grandiflora against Edwardsiella tarda using the well diffusion method in different concentration (75 ppm, 150 ppm dan 225 ppm), and knowing the active compounds of FTIR absorption bands. MIC (minimum inhibitory concentration) value of extract, n-hexane, ethyl acetate, and water fractions respectively was 0.51 %, 1.11 %, 0.97 %, dan 0.79 %. InIibitory zone diameter of the n-hexane fraction is most active against Edwardsiella tarda bacteria. The results of FTIR identification showed that n-hexane fraction contained flavonoids and terpenoids, as seen from absorption bands that function as antibacterial compounds.Keywords: antibacterial, Edwardsiella tarda, FTIR, Phytochemical Sesbania grandiflora.

Peranan Curcumin terhadap Proliferasi, Apoptosis dan Diferensiasi Hepatosit Mice Balb/C yang Dipapar dengan Benzapyrene Candra Rini; Edi Widjajanto; Rm. Loekito
The Journal of Experimental Life Science Vol. 1 No. 2 (2011)
Publisher : Postgraduate School, Universitas Brawijaya

Pengobatan neoplasma hepar belum memuaskan. Kini sedang dikembangkan pengobatan baru yang berasal dari alam, diantaranya curcumin (bahan aktif spesies Curcuma), yang dinyatakan mempunyai aktifitas anti kanker, dan penelitiannya masih dikembangkan. Tujuan dari penelitian ini adalah untuk membuktikan pengaruh anti-karsinogenesis curcumin pada proliferasi, apoptosis dan differensiasi hepatosit pada mencit yang dipapar dengan bahan karsinogen benzapyrene. Penelitian ini menggunakan 20 ekor mencit jantan (Mus musculus Outbred BABLB/c) usia 5 minggu, dibagi menjadi 4 kelompok yaitu kelompok normal, kelompok yang diinjeksi corn oil 3,2 ml tiap kgBB (i.p), kelompok dengan benzapyrene 16 mg tiap kgBB dalam corn oil secara intraperitoneal (i.p), dan kelompok yang diinjeksi benzapyrene dalam corn oil secara i.p dan diberi 0,2% curcumin dalam pakannya. Hari ke-120 mencit dikorbankan, organ hepar diperiksa secara histopatologis. Pemeriksaan proliferasi diukur dengan satuan indeks Cyclin-PCNA secara immunohistokimia. Apoptosis diukur dengan satuan indeks apoptosis, diferensiasi diperiksa dengan mengukur N/C ratio hepatosit, menggunakan pengecatan hematoxillin-eosin. Hasil penelitian ini menunjukkan bahwa pemberian 0,2% curcumin dalam pakan mencit yang dipapar benzapyrene menurunkan indeks Cyclin-PCNA (P<0,05), meningkatkan indeks apoptosis (P<0,01) dan menurunkan N/C ratio hepatosit (P<0,01) dibandingkan dengan mencit yang dipapar benzapyrene tanpa diberi curcumin. Kesimpulan penelitian ini bahwa pemberian curcumin selama periode inisiasi dan post-inisiasi terbukti memberikan efek anti kanker, ditandai dengan penurunan proliferasi, peningkatan apoptosis, dan perbaikan pola diferensiasi sel. Kata kunci: apoptosis, benzapyrene, curcumin, hepatosit

The Combination of Entomopathogenic Fungus of Beauveria bassiana (Balls) Vuill. with the Insect Growth Regulator (IGR) of Lufenuron Against Reproductive of Bactrocera carambolae Fruit Flies (Diptera: Tephritidae) Adrianto Marthinus Ndii; Bambang Tri Rahardjo; Toto Himawan
The Journal of Experimental Life Science Vol. 6 No. 1 (2016)
Publisher : Postgraduate School, Universitas Brawijaya

The study aimed to determine the reproductive ability of fruit flies B. carambolae treated with B. bassiana and Lufenuron. This study is conducted at the Laboratory of Pest. Department of Plant Pests and Diseases, Faculty of Agriculture, University of Brawijaya, Malang. This study used a completely randomized design with nine treatment and three replications. The study was trying to evaluate the effect of B. bassiana and Lufenuron on the reproduction capacity of B. carambolae. Results showed that adults of B. carambolae to applied combination of B. bassiana and Lufenuron immediately after coming out of the pupae until day eighth have the average number of eggs laid is 7.69%, a decrease of fecundity 92.40%, egg fertility by 61.38% and 95.24% decrease of reproduction. Adults of B. carambolae applied of B. bassiana and Lufenuron on day eighth until day sixteenth (for 8 days), show a decrease in the number of eggs laid by 13.63%, the decrease of fecundity 88.50%, egg fertility by 50.16% and decrease of reproduction by 93.12%.Keywords: Bactrocera carambolae, Beauveria bassiana, Lufenuron.

The Morphological Characters of The Malayan Pit Viper Calloselasma rhodostoma (Kuhl, 1824): on The Cephalic Scalation and Distribution Status in Indonesia Ahmad Muammar Kadafi; Amir Hamidy; Nia Kurniawan
The Journal of Experimental Life Science Vol. 8 No. 3 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

The examination on variations of morphological characters among 35 specimens of Calloselasma rhodostoma (Kuhl, 1824) from four different populations in Indonesia has been completed in this study. Univariate and multivariate analyzes allowed us to recognize the clustering of four populations through morphological diagnosis. The results of the average body size (Total Length) showed that the largest male is from Kangean Island (579.33 mm), while the largest female is from Java (841.07 mm). Comparison of meristic analysis represented three clusters from Principal Component Analysis (PCA) which is considered to be independent population. Here we also described three types of cephalic scalation variation that called small accessories scales and their distribution in Indonesia. Keywords: C. rhodostoma, Indonesia, Meristic, Morphometry, Viperidae.

Dried Skeletonema costatum in Feed Formulation for the Growth of Vaname Shrimp (Litopenaeus vannamei) Dewi Putri Lestari; Arning W. Ekawati; Maftuch Maftuch
The Journal of Experimental Life Science Vol. 4 No. 2 (2014)
Publisher : Postgraduate School, Universitas Brawijaya

The aim of this study was to determine the effect and the best dosage used by the Skeletonema costatum in feed formulation on the growth of Vaname shrimp. This experiment used Completely Randomized Design (CRD) with four treatments and three replications. Treatment was given by using dried S. costatum in feed formula (iso protein 37% and iso energy 3.6 kkal/g feed). Feed treatment was the substitution of fish meal protein A = 0%, B = 2.5%, C = 5% and D = 7.5% with dried S. costatum protein. The observed parameters include Survival Rate (SR), Survival Growth Rate (SGR), Feed Efficiency Ratio (FER), and Protein Efficiency Ratio (PER). The results showed that the use of dried S. costatum in feeding are effective in increasing the specific growth rate, feed efficiency ratio and the ratio of protein efficiency than controls. The best dosage in feeding formula is ranged from 4.39% to 4.75%.Keywords: feeding formula, Skeletonema costatum, Vaname Shrimp

Effect of Bulbils Position on Leaf Branches to Plant Growth Responses and Corms Quality of Amorphophallus muelleri Blume Retno Mastuti; Nunung Harijati; Estri Laras Arumingtyas; Wahyu Widoretno
The Journal of Experimental Life Science Vol. 8 No. 1 (2018)
Publisher : Postgraduate School, Universitas Brawijaya

Bulbil (corm leaf) is one material source of vegetative propagation in Amorphophallus muelleri Blume. Based on the position in branches there are two types of bulbil, middle and edge bulbils, which are different in shape and size. It has been reported that bulbil size affected not only the growth response of seedlings and plants but also the quality of the produced corms. Therefore, the objectives of this experiment were to study the effect of bulbils origin on A. muelleri Blume (Porang) growth and the quality of harvested corms. The quality of corms was assessed based on glucomannan and (CaOx) content. Bulbils from the middle and edge of branches were grown in polybag (f 5 cm) containing compost:fertilizer (1:1) mixed media under 40% shade. Each bulbil origin was repeated 8 times. The results showed that growth responses of plant from middle bulbil were significantly better than that from edge bulbil. However, at the harvesting time, the weight and diameter of corms derived from both types of bulbils were not significantly different. The glucomannan and calcium oxalate (CaOx) content of harvested corms grown from middle bulbil tends to be higher than that from edge bulbil.Keywords: branching, calcium oxalate (CaOx), germination, glucomannan, Porang.

Phytochemical Screening and Antibacterial Activity of Kratom Leaf (Mitragyna speciosa Korth.) Against Aeromonas hydrophilla Juanda, Eki; Andayani, Sri; Maftuch, Maftuch
The Journal of Experimental Life Science Vol. 9 No. 3 (2019)
Publisher : Graduate School, Universitas Brawijaya

Kratom (Mitragyna speciosa) is an indigenous tropical herbal plant to the Northern Malay Peninsula and Thailand. Empirically kratom leaves have several properties as herbal medicines. Currently, the treatment of diseases caused by bacteria that are resistant to antibiotics requires new compounds that have high potential. The material studied was kratom leaf extract. The solvent used for extraction is methanol. Phytochemical screening carried out includes the examination of alkaloids, flavonoids, steroids/terpenoids, phenols, tannins, and saponins. The test bacteria used was Aeromonas hydrophilla. The medium used is Nutrient Agar (NA). NaCl 0.9% as a bacterial suspension. Testing the antibacterial activity carried out with 10 (ten) variations of concentration namely 3%, 6%, 9%, 12%, 15%, 18%, 21%, 24%, 27% and 30 %. Fresh kratom leaf is collected and then wet sorted. The extraction process was using the method of maceration. This study aims to analyze the antibacterial activity of kratom leaf, measure the secondary compounds of kratom leaf extract, and measure the best concentration to kill Aeromonas hydrophilla. The extraction process obtained blackish-brown extract with 62.27 grams of extract (31.14%). Secondary metabolites of kratom leaf extract show positive results of alkaloid, saponins, tannins, phenolics, steroids, and triterpenoids. Antibacterial activity against Aeromonas hydrophila which is characterized by a kill zone around the paper disc with the best concentration that shows a wide killing zone was 24% of the extract.Keywords: Antibacterial, Extract, Kratom, Mitragynine, Phytochemical Screening.

Profil Gr-1 dan CD34 Mencit yang Diinfeksi Staphylococcus aureus Pacsa Pemberian Ekstrak Buah Mengkudu (Morinda citrifolia) Dia Faroka; Sri Rahayu; Muhaimin Rifa'i
The Journal of Experimental Life Science Vol. 3 No. 1 (2013)
Publisher : Postgraduate School, Universitas Brawijaya

S. aureus menyebabkan berbagai macam penyakit infeksi sistemik, seperti endokarditis, osteomielitis, sindrom kulit melepuh, pneumonia dan penyakit Toxic Shock Syndrom (TSS). Faktor virulen S. aureus dapat menginduksi peningkatan neutrofil, inflamasi, serta menstimulasi sel T sehingga terjadi sekresi citokin proinflamasi secara besar-besaran. S. aureus resisten terhadap antibiotic sehingga mendorong masyarakat untuk mencari tanaman obat tradisional. Tanaman obat lebih efektif, efek samping lebih kecil, dan harga lebih murah dibandingkan obat sintetik. M. citrifolia dijadikan bahan alternatif pengobatan karena memiliki potensi sebagai anti mikroba, anti kanker, anti inflamasi dan antioksidan. Penelitian ini bertujuan untuk mengetahui profil Gr-1 dan CD34 mencit yang diinfeksi S. aureus pacsa pemberian ekstrak air buah mengkudu (M. citrifolia). Penelitian menggunakan RAL factorial. Terdapat 2 kelompok yaitu kelompok non infeksi dan infeksi. Kedua kelompok diberi ekstrak air buah M. citrifolia dengan dosis berturut-turut 25 mg/kgBB, 100 mg/kgBB, dan 300 mg/kgBB selama 20 hari kemudian diinfeksi S. aureus sebanyak 1 x 109 sel. Deteksi jumlah relatif Gr-1 dan CD34 menggunakan Flow cytometry, dianalisis dengan progam CellQuest dan dilakukan uji statistik ANOVA dan uji BNJ menggunakan progam SPSS 16. Hasil penelitian menunjukkan bahwa, Pada kelompok non infeksi terjadi peningkatan Gr-1 pada dosis 100 mg/kgBB, dosis 25 mg/kgBB dan 100 mg/kgBB serta terjadi peningkatan dan penurunan CD34 secara signifikan (P<0.05). Pada kelompok Infeksi terjadi penuruan Gr-1 pada dosis 300 mg/kgBB, dan peningkatan CD34 pada dosis 100 mg/kgBB. Penurunan Gr-1 dimungkinkan karena senyawa M. citrifolia yang berperan sebagai anti inflamasi. Kata kunci: Morinda citrifolia, Staphylococcus aureus, Gr-1, CD34

Page 16 of 30 | Total Record : 299

14 15 16 17 18

Filter by Year

2011 2025

Filter By Issues

All Issue Vol. 15 No. 3 (2025) Vol. 15 No. 2 (2025) Vol. 15 No. 1 (2025) Vol. 14 No. 3 (2024) Vol. 14 No. 2 (2024) Vol. 14 No. 1 (2024) Vol. 13 No. 3 (2023) Vol. 13 No. 2 (2023) Vol. 13 No. 1 (2023) Vol. 12 No. 3 (2022) Vol. 12 No. 2 (2022) Vol. 12 No. 1 (2022) Vol. 11 No. 3 (2021) Vol. 11 No. 2 (2021) Vol. 11 No. 1 (2021) Vol. 10 No. 3 (2020) Vol. 10 No. 2 (2020) Vol. 10 No. 1 (2020) Vol. 9 No. 3 (2019) Vol. 9 No. 2 (2019) Vol. 9 No. 1 (2019) Vol. 8 No. 3 (2018) Vol. 8 No. 2 (2018) Vol. 8 No. 1 (2018) Vol. 7 No. 2 (2017) Vol. 7 No. 1 (2017) Vol. 6 No. 2 (2016) Vol. 6 No. 1 (2016) Vol. 5 No. 2 (2015) Vol. 5 No. 1 (2015) Vol. 4 No. 2 (2014) Vol. 4 No. 1 (2014) Vol. 3 No. 2 (2013) Vol. 3 No. 1 (2013) Vol. 2 No. 2 (2012) Vol. 2 No. 1 (2012) Vol. 1 No. 2 (2011) Vol. 1 No. 1 (2011) More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Jehan Ramdani Hariyati

Contact Email jehanramdani@ub.ac.id

Phone +6282333752235

Journal Mail Official jehanramdani@ub.ac.id

Editorial Address Jl. Veteran Malang 65145

Location Kota malang, Jawa timur INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Jehan Ramdani Hariyati

Contact Email
jehanramdani@ub.ac.id

Phone
+6282333752235

Journal Mail Official
jehanramdani@ub.ac.id

Editorial Address
Jl. Veteran Malang 65145

Location
Kota malang,

Jawa timur

INDONESIA