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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2,170 Documents
 133   134   135   136   137 
Larutan sukrose-elektrolit untuk rehidrasi pada diare P. A Moenginah P. A Moenginah
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 9, No 04 (1977)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Keywords: sukrose-elektrolit, glukose-elektrolit, rehidrasi
Cloning and expression of cDNA encoding 28 kDa soluble protein (GRA2) tachyzoite of Toxoplasma gondii local isolate Jarot Subandono Jarot Subandono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 2 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Toxoplasma gondii is an intracellular parasite that causes toxoplasmosis and found in tropical countries. Toxoplasmosis is dangerous if suffered by pregnant woman or immunodeficiency patients. T. gondii has 28 kDa soluble protein from dense granule (GRA2) and among GRA proteins, GRA2 is probably the most immunogenic. Disruption of the GRA2 locus in T. gondii has resulted in decreasing of the parasite virulence in mice. Invasion of tachyzoite T. gondii into macrophages was also significantly inhibited by anti GRA2 antibody, therefore the availability of GRA2 protein is essential for development of protective vaccine.Objective: The aim of this research was to produce 28 kDa soluble protein (GRA2) by cloning and expression of cDNA encoding soluble protein tachyzoite of T. gondii local isolate.Methods: Total ribonucleic acid (RNA) and messenger RNA was isolated from tachyzoite of local T. gondii grown up in Balb/c mice. Messenger RNA was isolated from total RNA using polyATtract mRNA Isolation Systems and synthesis of cDNA using Universal RiboClone cDNA Synthesis Systems. Recombinants of pUC18 were transformed into E. co/i XL1-Blue by heat shock technique. Expression of recombinant protein was analysed by immunoblotting using polyclonal antibodies against soluble protein of T. gondii. Results: Two recombinant clones were isolated which expressed 28 kDa recombinant protein.Conclusion: Two recombinant clones were isolated. The immunoblotting result indicates that the recombinant expressed 28 kDa recombinant protein which hybridized with the antibody polyclonal against soluble protein of T. gondii and the proteins are possibly GRA2 proteins.Key words : Toxoplasma gondii - tachyzoite - cDNA - 28 kDa soluble protein.
The benefits of steroid as therapy for tuberculous effusion Barmawi Hisyam Barmawi Hisyam
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 03 (2001)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Tuberculous effusion is a complication of pulmonary or extra pulmonary tuberculosis. The specific diagnosis is made by the finding of mycobacterium in the effusion and pleural tissue. Therapy of tuberculous effusion is mainly reducing symptoms, prevent progressivity and fibrothorax. Common complications of tuberculous effusion are pleural fibrosis, thickening and calcification of pleura. To prevent those, corticosteroid is often needed as additional therapy, besides anti tuberculous drugs. There has been controversy about the benefits of steroid as therapy for tuberculous effusion.Keywords : pulmonary tuberculosis, extrapulmonary tuberculosis, tuberculous effusion, corticosteroids, anti tuberculous drugs.,
CT scan examination on stroke patients Arif Faisal Arif Faisal
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 23, No 04 (1991)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

This study was conducted in patients with clinical stroke. The results showed that 61.3% patients had cerebral infarction, 33.0% hemorrhagic lesions and 5.7% was normal by CT scan examination. 12 of 29 hemorrhagic lesionswere associated with in [raven tri cular bleeding and these lesions have a poor prognosis.The cerebral infarctions were located mostly in capsula interna and nucleus le n tiforrnis, but the thalamus and capsula interna demonstrated as the main location for hemorrhagic lessons. The usefulness of CT scan in stroke lies especially in detecting the location, extension of lesion and in differentiating between ischemic and hemorrhagic lesions.Key Words: CT scan - in traventricular bleeding - hem iparesis - cerebral infarction - stroke
Intoleransi terhadap, air susu sapi (milk intolerance) Zainal Arifin Nang Agus Zainal Arifin Nang Agus
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 02 (1996)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Cow's milk has been recognised as an almost ideal food because it serves as a source of complete nutrients. Unfortunately, there are some people who cannot drink milk (milk intolerance) because lack of lactase.. High frequency of milk intolerance is found in developing or tropical countries and non-Caucasian race. It is a problem for public nutrition in those countries. Lactase is an inducible enzyme. The inductor is lactose, a major carbohydrate in milk. Some studies suggested that milk intolerance was influenced by genetic factors, diet and milk drinking habit. Efforts to overcome milk intolerance by providing low lactose milk had been done, but the result was not yet successful.Key words: protein calori malnutrition - cow's milk - lactase deficiency - milk intolerance
High number of granzyme B expressing CTLs predicts worst prognosis of nasopharygeal carcinoma patients Harijadi Harijadi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 40, No 01 (2008)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Nasopharyngeal carcinoma (NPC) characteristically harbors many tumor-infiltrating lymphocytes. In biopsies of Hodgkin or anaplastic large cell lymphoma many activated CTLs are related to a very poor clinical outcome, suggesting that in these cases with a strong CTL mediated anti-tumor cell response, selection occurs for tumor cells that have become resistant to CTL and chemo and/or radiotherapy induced apoptosis. Only activated CTLs and natural killer cells express granzyme B.Objective. Since, similar to lymphomas, the prognosis of NPC patients depends primarily on the sensitivity of tumor cells to radio- and/or chemotherapy, this study investigated whether the presence of many tumor-infiltrating activated CTLs in tumor biopsies also predicts poor prognosis in NPC patients.Methods: The study investigated 39 specimens of Indonesian NPC patients that fulfilled the following criteria; no evidence of distant metastases at the time of diagnosis, and complete remission following complete radio- and/or chemotherapy. Number of tumor-infiltrating activated CTLs was detected using a combination of antibodies against granzyme Band CD3, CD8 and CD56.Results: The presence of a high number of tumor infiltrating activated CTLs expressing granzyme B appeared to be a very strong predictor of a rapid fatal clinical outcome. Its prognostic value was stronger than and independent from the other prognostic makers; age and clinical TNM stage at presentation. Prognosis was found to decline strongly with increasing percentage of activated CTLs. The most informative cut-off value was found to be 25%. The median overall survival time of patients with
Karakteristik Anthropometrik Penduduk Pulau Palue Josef Glinka Josef Glinka
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 16, No 02 (1984)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The island of Palue belongs to the Lesser Sunda Archipelago on 121° 43' East and 8° 20' South, some 15 km north of Central Flores. The anthropometrical material given here was collected in 1966/67 by the author, At that time the island was a biological isolate. The number of individuals is 526 men and 296 women.Key Words: Indonesia - Lesser Sunda Islands - Palue - anthropometry - biological isolate
RESOCIALIZING EFFECT OF PIMOZIDE ON CHRONIC INSTITUTIONALIZED SCHIZOPHRENIC PATIENTS Soebagyo Hadinoto Soebagyo Hadinoto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 8, No 02 (1976)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

A clinical trial of pimozide has been conducted, on 20 chronic institutionalized schizophrenic patients. using the "same subject design". The aim was to check the resocializing effect of pimozide such as reported in same papers.A psychotest was constructed to obtain quantitative data concerning obedience and cooperativeness to do a task, work and work capacity. The quantitative data obtained were supplemented with the data observed qualitatively by the experimenting team.The effect of pimozide appeared to be positive and significant with minimal side effect
The description of concentration, viability, motility and normal sperm form in patients with varicocele. Dicky M. Rizal Dicky M. Rizal
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 35, No 2 (2003)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Varicocele was one of the causal factor s of infertility which related with the abnormality of sperm.Objective: The aim of this study was to know the description of concentration, viability, motility and normal sperm form in patient with varicocele, and different level of that result between three grades of varicocele.Method: This study was a retrospective-analytic study on the medical record data of patients with varicocele at Fermata Hati Infertility Clinic during January 2001-July 2002. The concentration, motility, viability and sperm normal form were evaluated and analized.Results: The results showed that 103 patients with varicocele were found, consisted of 87.4% patients with left varicocele, 1.9% right varicocele, and 10.7%. bilateral. The incidence of decreased concentration 38.8%, decreased motility 63.7% sibjects and viability 51.6%. There were decreased mean ratio of motility Ix = 41.12% for (a) category and x=16.37% for (b) category), and viability (x =73.5% life). Analysis by one way ANOVA showed that no significant differences of mean between each variable of three grades of varicocele (p>0.05).Conclusion: Abnormality in the parameters of sperm analysis was found in varicocele cases, but the grade of varicocele was not related to the concentration, motility, viability and normal sperm form.Keywords : varicocele-sperm motility-sperm viability-sperm form-infertility.
The efficacy of using intraoperative compared with postoperative topical 0.04% Mitomycin-C drops to the recurrence of Pterygium Suhardjo Suhardjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 03 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: The major problem in the treatment of pterygium is to prevent recurrences after surgical excision. To prevent the neovascularization and recurrence, variable doses and administration of mitomycin C installation had been used. However no single treatment has been universally effective yet.Objective: The purpose of this studies is to evaluate the safety and efficacy of two different methods of 0.04% Mitomycin - C application as adjunctive chemotherapy to the recurrence of pterygium. Methods: The research had been conducted at the Dr. Sardjito Central Hospital, Wates Central Hospital Gombong PKU Hospital, and Purworejo Aisyiah Hospital from January 1995 to December 1998. Eighty eight patients underwent pterygium excision were involved in this study, and divided into two groups. Group I or intraoperative 0.04% MMC group of 44 cases receiving 0.04% MMC drops applied to the bare sclera for 4 minutes intraoperatively. Group II or post operative group of 44 cases receiving 0.04% MMC eye drops one day after excision once daily during the first postoperatively week and continued with a dosage of one drop 3 times a day during the second postoperative week. Observations were done daily during the first postoperative week, weekly during the first postoperative month, and monthly up to 6 months. Observations were done including of the symptoms of recurrence and side effects of the treatment. The data were analyzed using the Student's test and chi square technique.Results: In the intraoperative group, 6 cases (13.67%) recurred, 2 cases of superficial punctate keratitis. In the postoperative group,. 7 cases (15.95%), and 3 cases superficial punctate keratitis. There was no statistical difference in the rates of recurrence (x2 ---- 0,0902, p>0,05). Most of the cases of pain, tearing and lid edema occurred in the group intra-operative. No other complication was observed in the course of the study.Conclussion: A single dose intraoperative application of MMC is a simple, economic, affective alternative adjunctive treatment for pterygium.Key words : Intraoperative mitomycin-C - pterygium excision - recurrence

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