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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2,170 Documents
 131   132   133   134   135 
Sarkoma jaringan lunak regio humeri. Penanganan dengan amputasi lengan atas Teguh Aryodono Teguh Aryandono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 28, No 01 (1996)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

The management of soft tissue sarcoma of the extremities is very complicated, particularly if the tumor is large and has infiltrated into the deep structures. Forequarter amputation is still one best choice for either curative or palliative purposes, although this operation is very mutilated.This report is aimed at presenting the benefit of forequarter amputation in patients with soft tissue sarcomas in the humeri region. Two patients with extrasceletal myxoid chondrosarcoma and pleomorphic rhabdomyosarcoma in the humeri region were managed with forequarter amputation and neoadjuvant chemotherapy. The patients condition and quality of life were better after the amputation.Key Words : soft tissue sarcoma - forequarter amputation - neoadjuvant chemotherapy - extrasceletal myxoid chondrosarcoma - pleomorphic rhabdomyosarcoma
he correlation between HPV infection and p53, pRb, KI-67 as well as tunnel expression of cervical carcinoma Irianiwati Ahmad Ghozali Endang Soetristi
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 39, No 03 (2007)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: HPV infection has an important role in cell proliferation and apoptosis of malignant neoplasia through the binding of their E6 and E7 protein to the p53 and pRb. In cervical cancer the role of HPV infection in cells proliferation and apoptosis has not yet been defined up to now, since mutation of p53 gene and Rb gene in cervical cancer is very rare.Methodes: Thirty-eight block parafin cases of squamous cell carcinoma of the cervix were stained immunohistochemically with polyclonal antibody against HPV and monoclonal antibody against p53, Rb, Ki-67 and Tunnel. with chromogen DAB and counterstain Hematoxyllin Meyer. The expression of HPV, p53, pRb, Ki-67 and Tunnel were counted based on the number of positive cells/100 tumor cells in five microscopic fields. The correlation between HPV expression and p53, pRb, Ki-67, Tunnel expression respectively were analyzed with Pearson correlation analysis.Results: There was a significant correlation between HPV expression with either p53 expressionR: 0.491, P:0.002) and or Ki-67 expression (R:0.397, P:0.014), but there was no significant correlation between HPV expression with pRB expression (R:0.3000, P:0.067) and Tunnel expression ( R:O,888, P:0,599).Conclusion: This research showed that HPV infection had important role in cell proliferation but not in cell apoptosis of cervical cancer.Key words: HPV, proliferation, apoptosis, p53, pRb, Ki-67, Tunnel, cervical carcinoma
Seurge- Weber Disease: A case report Sri Sutarni Sri Sutarni
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 15, No 03 (1983)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Sturge-Weber Disease is one of the congenital malforMations, in which we find naevus facialis, general or focal convulsion, mental retardation, and contralateral hemiparesis; sometimes also choroid angiomata, buphthalmus and glaucoma.Skull rbntgenology, a E. G. and cerebral angiography make the diagnosis clear.We reported on a boy, 5 years old, with Sturge-Weber Disease who came to the Hospital with convulsion.Key Words: Sturge-Weber Disease - congenital malfOrmation - naevus facialis - convulsion - mental retardation
Perobahan-Perobahan Neuroendokrinal dan Psikis Pada Masa Senium Radiopoetro Radiopoetro
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 7, No 04 (1975)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

key words: perubahan psikis pada usia lanjut
The frequence of human papillomavirus (HPV) DNA on cervical cancer patients from several hospitals in Yogyakarta by PCR test. Titik Nuryastuti Titik Nuryastuti
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 34, No 04 (2002)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background : Cancer of the uterine cervix (cervical cancer) is one of the most common malignant neoplasm in women in developing countries and remains a major public health problem worldwide. Several studies have proved the role of HPV as an agent STD (sexually transmitted disease), in the pathogenesis of Cervical cancer, beside the other risk factors.Objective : The aim of this study was to know the frequence of HPV infection in Yogyakarta.Material and Methods : 44 samples of Cervical cancer biopsies were collected from Sardjito General Hospital, Bethesda Hospital and private doctors, and stored frozen. DNA isolated from the biopsies of cervical cancer was amplified by PCR using consensus primer (CP I and CP IIG) from El ORF (188 bp) of HPV.Result : HPV DNA was detected in 84.1 % of the samples. In CIN I, 20 % samples were infected with HPV, 100 % in Ca epidermoid, 66.4 % in adeno Ca and 0 % in Ca adenosquamosaConclusion : The frequency of HPV in cervical Ca biopsies of certain histopathological status in Yogyakarta by PCR method was proved to be very high.Key words : HPV infection - cervical Ca - biopsy - PCR
Hyphaema in phacolytic glaucoma, a case report. Suhardjo Suhardjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 31, No 02 (1999)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: Phacolytic glaucoma is a secondary glaucoma which occurs in open angle hypermature cataract and caused by trabecular obstruction. This abstruction occurs due to the leaked of lens protein through an intact capsule. Hyphaema in phacolytic glaucoma is an unusual case.Objective : To evaluate the result of conservative therapy and extra capsular cataract extraction on phacolytic glaucoma associated with hyphaema.Methods :A case of hyphaema in phacolytic glaucoma has been reported in a 62 year old man. Symptoms of decreased visual acuity, pain, headache were found in this case. Ophthalmology examination found right visual acuity 1/", light projection and color perception good, conjunctival hyperaemia, ciliary injection, corneal edema, hyphaema, opacity of anterior chamber, iris atrophy, posterior synechiae, and increased intraocular pressure. This case was treated conservatively and cataract surgery was also done accordingly.Result: The result of treatment was loss of vitreous resulted from rupture of posterior capsule, decreased of intraocular pressure.Conclusion : Conservative therapy and extra capsular cataract extraction can improve visual acuity in phacolytic glaucoma associated with hyphaema.Key words: hyphaema - phacolytic glaucoma - conservative and cataract surgery - vitreous loss.
Diagnosis Histopatologis Lymphoma Non-Hodgkin Disesuaikan Dengan Pengelolaan Penderita Soeripto Soeripto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 22, No 01 (1990)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Rappaport non-Hodgkin lymphoma classification is well known, by pathologists and clinicians. This classification is based on the morphology of tumor cells. Lukes and Collins classification, and also Lennert classification are new non-Hodgkin lymphoma classifications. These classifications are based on morphology, basic function of tumor cells, and the clinical application. However, there are some differences between Rappaport classification, Lukes and Collins classification and Lennert classification. To compromise the differences of the classifications, there is a working classification of non-Hodgkin lymphoma which is used for clinical diagnosis.It is important to discuss the non-Hodgkin lymphoma classifications because each classification has its own basis. The basic classification of non-Hodgkin lymphoma is useful for the specialists based on their specialties; Lennert classification, and Lukes and Collins classification are the applicable classifications of non-Hodgkin lymphoma for pathologists and clinicians, while Rappaport classification is more useful for pathologists.Key Words: non-Hodgkin lymphoma histopathological diagnosis of lymphoma - basic function of tumor cells - leucaemia - Sezary's syndrome
Cell mediated immunity in malaria : 1. Changes in numbers of mononuclear phagocytes during plasmodium vinckei petteri infection in immunized and non-immunized mice Supargiyono Supargiyono
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 27, No 01 (1995)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Penelitian bertahap telah dilakukan'untuk mempelajari reaksi-reaksi immunitas selular yang terjadi selama infeksi Plasmodium vinckei petteri malaria pada mencit LACA. Fokus kajian pada penelitian tahap pertama ini adalah mempelajari profil perubahan jumlah sel fagosit mononuklear (mononuclear phagocytes) dalam sumsum tulang, darah tepi dan dalam limpa, selama infeksi malaria pada mencit yang diimunisasi dan yang tidak diimunisasi. Imunisasi dilakukan dengan penyuntikan secara intravena 108 parasit stadium darah yang dibebaskan dari eritrosit dengan larutan saponin dan difiksasi dengan larutan formalin. Kedua kelompok mencit, yang diimunisasi dan yang tidak diimunisasi diinfeksi dengan 103 eritrosit terinfeksi, pada hari ke 15 sesudah imunisasi. Jumlah sel fagosit berinti satu beserta sel prekursornya dievaluasi berturut-turut pada hari ke 0, 3, 6, 9 dan 12 sesudah infeksi. Hasil penelitian menunjukkan bahwa selama infeksi P. v. petteri, jumlah sel fagosit mononuklear meningkat di dalam sumsum tulang, kemudian di dalam darah tepi dan dalam limpa. Derajat peningkatan jumlah sel fagosit tersebut lebih tinggi pada mencit yang telah diimunisasi sebelumnya dibandingkan dengan pada mencit yang tidak diimunisasi. Tingginya peningkatan jumlah sel fagosit mononuklear pada mencit yang diimunisasi tersebut nampaknya berkaitan dengan keberhasilan sistem kekebalan tubuh hospes dalam menurunkan parasitemia. Peningkatan jumlah sel fagosit tersebut didahului dengan peningkatan jumlah sel prekusornya di dalam sumsum tulang, sehingga bisa diantisipasikan bahwa produksi dan proliferasi dari sel prekursor di dalam sumsum tulang juga meningkat dalam usaha memenuhi kebutuhan sel d.ewasa di dalam darah dan jaringan selama infeksi malaria.Key words: Plasmodium vinckei petteri -- malaria -- LACA mice -- mononuclear phagocyte -- cellular immunity
Effect of temperature and storage duration of Aedes aegypti mosquito specimens artificially infected with dengue-3 virus on the results of immunohistochemical examination Susilawati Sitti Rohmah Umniyati Tri Baskoro Tunggul Satoto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 44, No 01 (2012)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

To confirm the presence of any dengue viral in a mosquito, mosquito’s head can be squashed ona slide and stained with immunohistochemical staining. The remaining samples then can bestored in the cryo freezer at -80ºC to avoid specimen damage. However, for laboratories withlimited facilities, with only a refrigerator with a temperature range of -20ºC to 4ºC is available,examination to evaluate whether the dengue antigen can still be detected in specimens storedat these temperature is necessary. It was a quasi-experimental study. Three to five-day-old adultfemale Aedes aegypti mosquitoes were injected intrathoracically with dengue-3 (DENV-3) strainH-87 virus and then maintained for about 7 days. The dengue viral antigen on mosquitoes wasidentified using immunohistochemical method after stored at a temperature of 4oC, -20oC, -80oCfor 2, 4 and 8 weeks. Mosquito specimens that were not stored were used as a positive control.Kappa value was counted to analyze level of agreements between two observers. Two-wayAnova was used to analyze mean positive rates. Kappa value showed poor agreement (0.00-0.16) between two observers when the specimens were stored at 4oC for 2-8 weeks, and showedgood agreement (Kappa value of 0.77), when stored at -20oC for 4 weeks. The kappa valueshowed very good agreement (0.90-0.92) when the specimens were stored at -20oC for 2 weeks,and at -80oC for 2-8 weeks. Mean positive rates of the specimens stored at 4oC were significantlylower (p<0.005) than stored at -20oC and -80oC, but there were no significant differencesbetween specimens stored at -20oC and -80oC (p>0.05). In conclusion, availability of thedengue viral antigen on mosquito specimens was influenced by temperature and storage durationof the specimens.Keywords: dengue virus - head squash - immunohistochemistry - kappa agreement - temperature
Penicillin Dalam Pengobatan Infeksi Meningococci Samekto Wibowo Samekto Wibowo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 6, No 02 (1974)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Banyak obat yang dipakai dalam pengobatan infeksi meningococci, di antaranya ialah penicillin. Obat pilihan bukan saja harus mempunyai effektivitas terhadap microorganismenya, juga harus mempunyai kemampuan masuk dalam cairan cerebrospinal. Inflamasi meninges memperbesar pemasukan obat ke dalam cairan itu.Banyak obat masuk dalam golongan penicillin, tapi semuanya termasuk golongan yang sukar menembus barrier darah-otak. Amoxycillin, suatu preparat penicillin semisynthetis yang baru, mempunyai kemampuan masukdalam cairan cerebrospinal yang lebih besar. lagi bila diberikan secara infus intraveneus yang lambat. Percobaan telah dilakukan pada anjing. Seandainya hal itu juga berlaku pada manusia, tentulah akan sangat menggembirakan.

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