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Journal of the Medical Sciences (Berkala Ilmu Kedokteran)
Published by Universitas Gadjah Mada
ISSN : 01261312     EISSN : 23563931     DOI : http://dx.doi.org/10.19106/JMedSci005303202101
Core Subject : Science,
Immunology & microbiology Neuroscience
Journal of the Medical Sciences (JMedSci) or Berkala Ilmu Kedokteran (BIK) is an international, open-access, and double-blind peer-reviewed journal, published by Faculty of Medicine, Public Health and Nursing, Universitas Gadjah Mada Yogyakarta Indonesia. JMedSci aiming to communicate high-quality articles in the areas of biomedical science from basic to clinical sciences.The journal welcomes papers from original articles, case reports, reviews, and book reviews. All papers published in JMedSci are freely available as downloadable pdf files. The journal began its publication on March 1973 and published quarterly (January, April, July, and October). JMedSci is abstracted and indexed in DOAJ, Crossref, Google Scholar, Sinta, Indonesia One Search. JMedSci is accredited by Directorate of General Higher Education, the Ministry of Research, Technology, and Higher Education, Indonesia
Arjuna Subject : Kedokteran - Kedokteran (Lain-Lain)
Articles 2,170 Documents
 96   97   98   99   100 
The protective effect of sunscreens against ultraviolet B-induced immunosuppression. A study on Langerhans cell depletion Flora Ramona SP Fajar Waskito Yohanes Widodo Wirohadidjojo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 42, No 01 (2010)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Ultraviolet B (UVB) radiation can act as immunosuppressant by inducing an epidermal Langerhans cells (LC) depletionwhich could be inhibited by topical sunscreen. Several kinds of sunscreens with various SPF (Sunscreen ProtectionFactor) are now available. The minimal SPF which able to inhibit the immunosuppressive effect of UVB amongpeople with skin photo-type IV has not been established yet. The aim of this study is to determine the minimal SPFcapable to inhibit UVB-induced immunosuppression among people with skin photo-type IV. A simple experimental(post test only experimental) study was conducted among 5 people’s circumsized foreskins with Fitzpatrick’s skinphoto-type IV. Each of them was divided into equal 5 pieces of 0.5 cm2. Each of three pieces of skin was treated bysunscreen SPF 15, SPF 30, and SPF 50, a single piece of skin was treated with placebo, and all of them then weretreated by a single 100 mJ/cm2 of UVB 30 minutes later. A rest single piece of skin was used as control. After 24hours of incubation in incubator of 37O C and 5% CO2, all of them then was fixed by buffer formalin, blocked byparaffin, cut in 2mm of thickness, and then stained with anti CD 1a antibody with AEC as chromogen and Mayer’shematoxylin as counterstaining. The number of LC was counted by Image J Analysis programmed and the mean ofLCwere analyzed by Kruskal-Wallis test dan Mann-Whitney test. There were very significantly different of themean number of LC between UVB placebo group and control group (p < 0.01). Compared to the control group,mean number of LC among SPF 30 and SPF 50 treated groups were not significantly different (p>0.05). Sunscreenwith SPF 15 had LC number lower than control group significantly (p<0.05). The lowest SPF for preventing UVBinduced LC depletion among people with skin photo-type IV was 30.Key words : UVB - immunosupression – sunscreens – SPF -CD1aexpression
ILEUS (intestinal obstruction) M. Soegijanto M. Soegijanto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 5, No 01 (1973)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Telah disampaikan keadaan penderita Liens yang pernah dirawat di Bagian Bedah Rumah Sakit Universitas Ga -djah Mada selama tahun2 1961 s/d 1970, dimana terdapat 378 penderita. Angka-mOrtalitas penderita ileus pada tahun2 tsb. masih tinggi, yaitu 28,83 %.Telah disampaikan pula sebab2 kematiannya,yang pada umumnya karena gangren uses dan keadaan umum yang jelek karena dehydrasi. Disamping itu telah disampaikan juga carat diagnose yang biasa di pakai di Rumah Sakit bniversitas.Gadjah Mada Bagian Bedah.
Satu Generasi Telah Lewat Anonim Anonim
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 25, No 01 (1993)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Kata Kunci: Redaktur
The impact of nosocomial postoperative wound infection on the cost of hospitalization in Obstetric and Gynecologic Unit, Sleman General Hospital,1992/1993 Stephani M Stephani M
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 29, No 01 (1997)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Nosocomial postoperative wound infection is a combined clinical and managerial problem. This report discusses the impact of nosocomial postoperative wound infection on the cost of hospitalization, in the Obstetric and Gynecologic unit, Sleman General Hospital in 1992/1993. There are two methods of nosocomial postoperative wound infection examination used in this study, namely: (1) comparative analysis among infected with uninfected and (2) subjective comparison study. The result of the first method showed a total cost increase of Rp. 74,877.00 and subjective comparison yielded total cost increase of Rp. 3,112,371.80. Based on the diagnosis, the additional cost of nosocomial infection is as follows: (1) for Cephalopelvic Disproportion; Rp. 224,081.61; (2) for twin pregnancy, premature rupture of membranes, and prolonged pregnancy; Rp 290,457.09; (3) for uterine myoma (30 hospital days), the additional cost of nosocomial infection is Rp 513,564.66; (4) for premature rupture of membrane, and fetal distress, Rp. 75,793.87; (5) for malignant neoplasm of ovary; Rp. 328,838.70; (6) for malignant neoplasm of uterine cervix, Rp. 374,326.44; (7) for premature rupture of membranes, prolonged first stage and failure of vacuum extraction, the additional cost of nosocomial infection is Rp. 251,563.22; (8) for premature rupture of membranes, failure of induction of labor by medical methods and fetal distress, Rp. 177,619.35; (9) for uterine myoma (12 hospital days), 876,126.86. It is recommended, therefore, that nosocomial infection control committee should be established in Sleman General Hospital. The Committee will assist the hospital director in planning, implementing, monitoring and evaluating nosocomial infection control activities. A social marketing program to improve aseptic techniques, the use of appropriate antiseptic and the maintenance of sterility in the operating room should be implemented so that nosocomial infection can be prevented and may reduce the cost of hospital care.Key words: nosocomial infection - post operative wound infection - cost of treatment - comparative analysis - subjective comparison
Standardization of Immunocytochemical method for the diagnosis of Dengue Viral Infection in Aedes aegypti Linn Mosquitoes (Diptera Culicidane) Sitti Rahmah Umniyati
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 41, No 01 (2009)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

methods for virus detection in the mosquito, such as the direct fluorescent-antibody test on head squashes. However, it has the disadvantages of being labor-intensive and requiring fluorescent microscope as well as cryofreezer. Newer methods involving enzyme conjugates such as peroxidase in conjunction with either polyclonal or monoclonal antibodies are greatly improved. With new methods of immunocytochemistry, it is now possible to detect dengue viral antigen in a variety of tissues. Objective: This study was aimed to standardize an immunocytochemical streptavidin-biotin-peroxidasecomplex assay for diagnosis of dengue infection in Aedes aegypti using monoclonal antibody DSSC7. Methods: The infected mosquitoes were held in small cylindrical cages covered with mosquito netting, and incubated at 27:t 1°C ami at relative humidity of 88:t 6 %. The specificity of the immunocytochemical procedure was validated by negative and positive controls showing that the antibody was bound to an appropriate structure. The sensitivity and specificity were also evaluated based on Herrmann's Formula. The presence of dengue antigen on head squash preparation was detected based on ISBPC assay using monoclonal antibody against dengue. The validity and reliability of the measurement were evaluated based on kappa values, according to Landis and Koch. Result: Positive result was detected as discrete brownish granular deposits throughout most visual fields of brain tissue. Dengue viral antigen was immunolocalized to the cytoplasm of brain cells. The immunocytochemical test under light microscope at magnification of 400x was 86.67% sensitive, 96.00% specific, and the kappa value is 0.64. Meanwhile the kappa value between two observers was 0.92, with sensitivity and specificity of 96% and 97% respectively at magnification of 1000x. Conclusion: The monoclonal antibody DSSC7 was sensitive, specific, valid, and reliable as primary antibody to detect dengue viral infection in Ae. aegypti head squash preparation based on immunocytochemical streptavidin-biotin-peroxidase-complex assay under light microscope. Key words: antigen - denguevirus - Aedes aegypti - immunocytochemistry - monoclonal antibody DSSC7
Retinochoroiditis Toxoplasmika Kongenital yang Menyebabkan Juling Nunuk Maria Ulfah, Suhardjo Nunuk Maria Ulfah, Suhardjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 20, No 02 (1988)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Four cases of 1- to 12-year old children with strabismus were reported. In all cases no abnormality of both the motoric and the refractive media of the eye were found. On the other hand, funduscopic examination revealed macular and retinal lesions in the form of rosette pigmentation and pseudocoloboma which were suggestive of the existence of congenital toxoplasmic retinochoroiditis.This suggestion was confirmed to be true with serologic examination for Taxopla,sma. It was thought that the squintness was secondary to the congenital toxoplasmic retinochoroiditis and possible mechanism was discussed.Key Words: toxoplasmic retinochoroiditis — disturbance of binocular vision — strabismus — rosette pigmentation — latex agglutination
Status asthmaticus Hindardjo Hindardjo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 9, No 03 (1977)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

keywords:asthma bronchiale, medical emergency
Risk factors of the faiture of vaginal delivery after previous Cesarean section history. Heru Pradjatmo Heru Pradjatmo
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 36, No 3 (2004)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Objective: To know factors influencing the success rate of vaginal delivery after previous Cesarean section history.Methods: Historical cohortSetting: Sardjito General Hospital YogyakartaParticipants: Women who delivered her child in Sardjito General Hospital between the year 1997 to 2001, and had previous Cesarean section history. Their delivery would be vaginally or recesarean section. The factors which were predicted affect the success of the vaginal delivery will be analyzed. The significance level of Odd Ratio was determined by logistic regression analysis.Results: There were 275 pregnant women with previous Cesarean section history reviewed, 110 (40%) patients successfully delivered the fetus vaginally and 165 (60%) patients unsuccessfully delivered the fetus vaginally and were undergone Cesarean section. Several factors that might influence the success of vaginal delivery were analyzed: place (OR =0.97; CI 0.58-1.65), age of the mother (OR =0.74; CI 0.431.29), mother body length (OR =1.15; CI 0.66-2.0), education of the mother (OR =1.41; CI 0.74-2.69), pregnancy interval (OR =0.83; CI 0.47-1.461, number of antenatal care (OR =4.40; CI 0.45-35.85), gestational age (OR =0.52; CI 0.24-1.12), fetal presentation (OR =0.61; CI 0.24-1.57), fetal body weight (OR =0.43; CI 0.21-0.89), history of vaginal delivery (OR =0.86; CI 0.51-1.43).Conclusions: It seems that only fetal body weight had significant influence to the success of the vaginal delivery after previous Caesarean section history. Anyhow, this result is appropriate to encourage a possible trial of vaginal delivery in almost all patients with a previous low-segment Caesarean section.Key words: Caesarean Section History, trial vaginal delivery, re-Caesarean Section
Prognostic significance of argyrophilic nucleolar organizer region (AgNOR) counts and nutritional status in pediatric acute lymphoblastic leukemia (ALL). Wisman Herminto Wisman Herminto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 33, No 03 (2001)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

Background: The cell proliferation rate is a well-established prognostic factor in cancer, but it has not been considered as a prognostic factor used to stratify ALL patients into risk groups.Objective: The main goal of this study was to verify the relationship between AgNOR number and nutritional status, at the time of diagnosis, and remission induction response rate and survival in ALL patients. Methods: Smears of marrow aspirates from 35 newly diagnosed and previously untreated ALL patients were stained, at presentation, by silver method and evaluated by counting the mean AgNOR number of each case. Anthropometric nutritional status was obtained also for each patient.Results: The mean AgNOR number of the whole series was 3.54 ± 0.74. It was significantly higher in resistant patients than in patients who achieved complete remission (p = 0.01). It was found that the mean AgNOR number = 4 was related to the lower remission induction response than the mean AgNOR number < 4 (p = 0.02). Multivariate analysis by Cox regression model showed that the mean AgNOR number retained its prognostic significance as a predictor of survival (p = 0.04). Conversely, nutritional status was not correlated with remission induction response, and was not of prognostic significance, either.Conclusion: AgNOR number at diagnosis is a reliable prognostic parameter to predict remission induction response rate and survival in childhood ALL, and should be rountinely introduced in the group risk definition.Keywords: AgNOR - nutritional status - prognostic factor - pediatric ALL
Klinefelter syndrome Ibnu Pranoto Ibnu Pranoto
Journal of the Medical Sciences (Berkala Ilmu Kedokteran) Vol 23, No 03 (1991)
Publisher : Journal of the Medical Sciences (Berkala Ilmu Kedokteran)

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Abstract

A 10-year old boy presented small testicles, a small penis, hypospadia, scanty hair growth, and long legs and arms indicating Klinefelter syndrome. Klinefelter syndrome is one of the abnormality of excessive number of sex chromosomes in which the male phenotype presents female characteristics such as breast development, scanty hair growth, long arms and legs, small testes and high pitch voice.The examination in the above case reveals two X-chromosomes and one Y-chrtimosome in the nuclei of cells as the result of fertilization from XX ovum and Y spermatozoa or from X ovum with XY spermatozoa!. Research studies showed there were 70% XXYmale cases in which their XX chromosomes came from the mother, whereas the other 30% cases their XYchromosomes came from the father. Other studies showed the poSsible relationship of Klinefelter syndrome with the incidence of leukemia.The result of chrothosomal analysysindicates that the abOve case was trUIYKlinefelter syndrome.Key Words: Klinefelter syndrome - genital abnormality - XY chromosomes - genetic mapping - clinical generics

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