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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 22 Documents
 1   2   3 
Search results for , issue "Vol 10, No 3 (2005): October 2005" : 22 Documents clear
Kualitas Minyak Kelapa Hasil Fermentasi Saccharomyces cereviceae Mursyanti, Exsyupransia
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2880

Abstract

One aspects of utilization of coconut fruit is its palm oil. Traditionally coconut oil was made by “klentik” method, which was time and energy consuming. The important thing in the production of coconut oil from its xanthan is the separation of protein from oil. One of separation methods is by creating low pH (acid) condition that is near to protein isoelectric point. This research objective was to determine the potency of Saccharomyces cereviceae, that has an optimum growth pH within the range of protein isoelectric point, to produce coconut oil. Three isolates of Saccharomyces were used, i.e. isolate A that was coming from alcohol industry (S. cereviceae strain Kyowa), isolate B that was natural type (isolated from fruits), and isolate C that was S. cereviceae in the form of powder (commercial isolate, Saf-instant). Coconut xanthan used in the research was prepared from old coconut fruits. Research stages including pure culture test of S. cereviceae, starter preparation, xanthan production, fermentation process, oil separation and heating. Quantitative and qualitative analyses were done for oil rendement, water content, free-fatty acid concentration, saponification number, iod number, and organoleptic test (color and aroma). The results showed that the quality of coconut oil produced by fermentation process was better than traditional way in term of rendement value, water content, saponification number, and iod number. This was supported also by organoleptic test that aroma and color of coconut oil from fermentation were more preferred by the panelists. Furthermore, the quality of oil produced by S. cereviceae fulfilled the standart stated by the Indonesian Department of Commerce (SII-1976) except for saponification number.
Komposisi Vegetasi Mangrove di Muara Sungai Siganoi Sorong Selatan - Papua Yohanes Y. Rahawarin
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2872

Abstract

The research was aimed to figure out the structure and composition of mangrove species in the delta of Siganoi River, Inanwatan District, South Sorong Regency, Papua. Belt transect was used and the data was analyzed using formula according to Clintron and Novelly. Research showed that ten species (seven genus) from five families were found in this area. At tree and sapling stages, Sonneratia alba performed the highest density with 324,1 trees/ha and 765,5 sapling/ha respectively, while at seedling stage Aegiceras corniculatumc with 69 seedling/ha. The highest frequency was found on trees and sapling of Sonneratia alba 0,966 and 0,431 respectevely. At the seedling stage, the highest frequency was found on species Aegiceras corniculatum (3970).
Metode Antagonistik Langsung untuk Skrining Bakteri Laut Penghasil Senyawa Anti-methicillin-resistant Staphylococcus aureus (MRSA) Alim Isnansetyo; Yuto Kamei
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2873

Abstract

Metode yang berbasis antagonistik langsung antara bakteri laut dan Staphylococcus aureus resistan metisilin (Methicillin-resistant Staphylococcus aureus, MRSA) berhasil digunakan untuk skrining bakteri laut penghasil senyawa anti-MRSA. Metode ini menggunakan medium agar lapis ganda untuk mendukung pertumbuhan bakteri laut dan MRSA. Beberapa bakteri laut penghasil senyawa anti-MRSA berhasil diisolasi dengan metode ini. Suatu bakteri laut, strain O-BC30 merupakan bakteri yang paling kuat menghambat pertumbuhan MRSA. Isolat-isolat bakteri yang didapat menunjukkan aktivitas yang stabil terhadap MRSA dan aktivitas ini juga konsisten dengan aktivitas ekstrak yang diperoleh dari sel bakteri yang dikultur pada medium agar. Metode ini sangat berguna dan efisien untuk skrining bukan hanya bakteri penghasil senyawa anti-MRSA tetapi juga bakteri penghasil antibiotik.
Penghambatan Produksi Aflatoksin dari Aspergillus flavus oleh Kapang dan Khamir yang Diisolasi dari Ragi Tempe Ekawati Purwijantiningsih; Ratih Dewanti-Hariyadi; C. C. Nurwitri; Istiana (alm.) Istiana
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2874

Abstract

This research was carried out to study the inhibition of aflatoxin production of Aspergillus flavus by moulds and yeasts isolated from ragi tempe. The result showed that moulds found in the ragi tempe consisted of Rhizopus oligosporus, R. oryzae, R. cohnii, Mucor rouxii, Rhizomucor pusillus, Aspergillus niger and miselia sterilia. Yeasts found in the samples were Candida sp1, Candida sp2 and Trichosporon sp. Twelve mould, and yeast isolates were chosen to determine their ability on inhibition of aflatoxin production. Aspergillus flavus alone or in association with each of these isolates were inoculated in Potato Dextrose Broth and analysed for aflatoxin production at day 10. All combinations yielded less aflatoxin than the single culture of A. flavus. All isolates tested could inhibit aflatoxin production. The highest inhibition was shown by Candida sp1 (99,96%).
Analisis kandungan artemisinin pada kultur tunas Artemisia annua L. dengan lima karakter morfologi yang berbeda Tri Muji Ermayanti; Erwin Al Hafiizh; Aryanti Aryanti; Lenny Sutedja
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2875

Abstract

Artemisia annua L. is a medicinal plant that has an antimalarial drug againts Plasmodium falciparum strains which are resistant to traditional drugs such as quinine and chloroquine. This plant contains artemisinin as major compounds found on its leaves. Aim of the research was to analyze the artemisinin content of shoot culture of A. annua which has five different in leaf morphologies (Morphology type A, B, C, D and E). Previous investigation showed that shoot regenerated from callus has five different morphologies. This somaclonal variation was permanent after continuously subcultured onto MS medium with no addition of plant growth regulators. Artemisinin was detected using HPLC and it was comfirmed by LC-MS. The results showed that shoot culture type B contained the highest artemisinin content (0.171%) amongst other type of morphology. Shoot type D showed the lowest level of artemisinin (0.006%) which was not significantly different with type E (0.008%). Addition of BAP at concentration of 1 and 2 mg/l increased artemisinin level of the shoot culturs.
Klorofil Floresen dari Spesies C3 dan C4 dalam Responnya terhadap Cekaman Kekeringan Hamim Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2876

Abstract

Klorofil floresen fotosintesis dari dua spesies C3 (gandum dan kale) dan dua spesies C4 (Echinochloa cursgallii dan Amaranthus caudatus) dianalisis dalam responnya terhadap cekaman kekeringan di rumah kaca. Tumbuhan ditanam dalam pot berdiameter 15 cm selama sebulan kemudian diberi perlakuan kekeringan dengan penundaan penyiraman hingga tumbuhan layu. Kuantum efisiensi maksimum dari sistem cahaya II fotosintesis (Fv/Fm), quenching foto kimia (qP) dan non-fotokimia (qN) dianalisis untuk mengetahui keadaan fotosintesis tumbuhan selama cekaman kekeringan. Walaupun tidak ada pola yang jelas dalam hal status air dari spesies C3 dan C4, cekaman kekeringan yang diberikan menyebabkan penurunan kadar air medium (MWC), potensial air (WP) dan potensial osmotik (OP) semua spesies.  Gandum mamiliki nilai WP dan OP yang paling rendah sementara E. crusgallii memiliki nilai yang paling tinggi akibat cekaman kekeringan. Kekeringan menyebabkan penurunan laju fotosintesis pada semua spesies yang ditandai dengan penurunan qP, namun hanya kale dan A. caudatus yang mengalami peningkatan qN akibat cekaman kekeringan. Tetap stabilnya qP dari E. crusgallii pada awal cekaman mungkin berkaitan dengan mekanisme C4 yang dimiliki oleh spesies ini.  Penurunan Fv/Fm pada E. crusgallii pada periode akhir cekaman menunjukkan bahwa spesies ini mengalami fotoinhibisi disebabkan cekaman kekeringan.
Studi Perilaku Teritorial Burung Cangak Abu (Ardea cinerea Linn.) di Kebun Raya dan Kebun Binatang Gembiraloka Yogyakarta Wahyu Nugroho; Djuwantoko Djuwantoko; Wibowo N. Jati
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2877

Abstract

This study aim to see on ecological aspect of Cangak Abu specifically on its behavior in defending their terittory. This research was done from July to August 2004. Data collecting was done using territory mapping methods with three observation area (1st-3rd). One pair of Cangak Abu observed at each area. Cangak Abu showed some behavior such as vocalization, sound, visual display, visual display-sound, visual display-sound-shortflight, patroling flight and fighting. The territories on frist to third observation areas were OA : 9 m2; OA : 6 m2 and OA : 9 m2 respectively.
Pengaruh Perbedaan Jarak Tanam terhadap Pertumbuhan Tiga Varietas Kedelai (Baluran, Bromo, dan Galunggung) Djukri Djukri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2878

Abstract

The objective of the research was to investigate the effect of sunlight radiation distribution in canopy on soybean plants biomass at different planting distances. This research was done experimentally using the Block Design. The independent variables were the soybean varieties and planting distance. The varieties used were Baluran, Bromo, and Galunggung and the planting distance used was 15x15 cm2 and 25x25 cm2 as blocks with 10 replications. The dependent variables were sunlight intensity and the plants biomass. The result of the research indicated that after 40 planting days, the sunlight intensity in the canopy, at soybean varieties factors was significant (p<0.05), and the planting distance was significant too. The plant biomass indicated that varieties factors had a significant effect (p<0.05), but the planting distance factors were not significant (p>0.05).
Indeks Glisemik Kacang Buncis (Phaseolus vulgaris) dan Kacang Panjang (Vigna sesquipedalis) serta Uji Efek Hipoglisemiknya pada Tikus Sprague Dawley Y. Marsono; Priyanto Triwitono; Sri Kanoni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2879

Abstract

Glycemic index (GI) of foods has been suggested as an additional parameter in selecting foods for the diabetic patients in nutritional management. GI was measured as the area under the postprandial blood glucose curve for a food expressed as a percentage of the area after the consumption of reference food (bread). In this research two legumes were investigated including snap bean (Phaseolus vulgaris L.) and yardlong bean (Vigna sesquipedalis). The objectives of this research were (i) to determine the IG of the beans and (ii) to investigate the effects of these legumes on serum glucose concentration of alloxan-induced diabetic rats. Ten health and normal volunteers (not diabetic) were provided. The volunteers took an overnight fasting, blood were drawn in the morning and analyzed for serum glucose. Then they were given the test beans containing total carbohydrates equivalent to 25-g glucose to be consumed. Blood samples were drawn for glucose measurement every 30 minutes until 120 min after meal. Serum glucose was determined enzymatically and the glucose responses were drawn graphically to calculate the GI of the beans. These beans were evaluated for their hypoglycemic properties in alloxan-induced diabetic rats. They were fed modified AIN 93 diets contain 20% of energy derived from snap bean or yardlong bean for 28 days. Serum glucose concentrations were measured weekly. It was found that with white bread as the food standard the GI of the snap bean and yard long bean were 43 and 86, respectively. In 28 days of diet intervention, snap bean decreased the serum glucose level by 73% (from 212mg/dL to 56mg/dL). Similar effect were found for the yardlong bean. This bean can reduced serum glucose level about 73% (from 224mg/dL top 61mg/dL), but both were similar to control diet that decreased the serum glucose level about 72% .
Kualitas Minyak Kelapa Hasil Fermentasi Saccharomyces cereviceae Exsyupransia Mursyanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24002/biota.v10i3.2880

Abstract

One aspects of utilization of coconut fruit is its palm oil. Traditionally coconut oil was made by “klentik” method, which was time and energy consuming. The important thing in the production of coconut oil from its xanthan is the separation of protein from oil. One of separation methods is by creating low pH (acid) condition that is near to protein isoelectric point. This research objective was to determine the potency of Saccharomyces cereviceae, that has an optimum growth pH within the range of protein isoelectric point, to produce coconut oil. Three isolates of Saccharomyces were used, i.e. isolate A that was coming from alcohol industry (S. cereviceae strain Kyowa), isolate B that was natural type (isolated from fruits), and isolate C that was S. cereviceae in the form of powder (commercial isolate, Saf-instant). Coconut xanthan used in the research was prepared from old coconut fruits. Research stages including pure culture test of S. cereviceae, starter preparation, xanthan production, fermentation process, oil separation and heating. Quantitative and qualitative analyses were done for oil rendement, water content, free-fatty acid concentration, saponification number, iod number, and organoleptic test (color and aroma). The results showed that the quality of coconut oil produced by fermentation process was better than traditional way in term of rendement value, water content, saponification number, and iod number. This was supported also by organoleptic test that aroma and color of coconut oil from fermentation were more preferred by the panelists. Furthermore, the quality of oil produced by S. cereviceae fulfilled the standart stated by the Indonesian Department of Commerce (SII-1976) except for saponification number.

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