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Brigitta Laksmi Paramita
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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,183 Documents
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Pengaruh Pupuk Organik Cair (POC) Terhadap Perkecambahan Cendana (Santalum album L.) Secara In Vitro di Nusa Tenggara Timur Solle, Hartini Realista Lydia; Nitsae, Merpiseldin; Ledo, Mellissa Erlyn Stephanie
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 4, No 3 (2019): October 2019
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (586.725 KB) | DOI: 10.24002/biota.v4i3.2517

Abstract

Cendana (Santalum album L.) merupakan tanaman hutan penghasil kayu di provinsi Nusa Tenggara Timur (NTT) dan mempunyai nilai ekonomi tinggi. Tanaman ini merupakan spesies endemikyang mempunyai keunggulan kadar minyak dan produksi kayu teras yang baik. Minyak cendana yang dihasilkan memiliki aroma yang harum sehingga banyak digemari. Kayu cendana digunakan sebagai bahan dasar parfum, sabun, ukiran dan kemenyan. Keberadaan cendana di NTT saat ini jumlahnya semakin menurun karena eksploitasi oleh penduduk setempat. Upaya pemulihan tanaman cendana NTT telah banyak dilakukan, seperti usaha pengembangan dengan penanaman cendana dari pembibitan maupun pemeliharaan anakan yang berasal dari penyebaran secara alami akan tetapi usaha tersebut tidak cukup berhasil. Untuk memecahkan masalah tersebut, perbanyakan tanaman melalui kultur jaringan menjadi salah satu cara terbaik dalam upaya konservasi. Tujuan penelitian ini untuk mengetahui pengaruh pemberian POC terhadap pertumbuhan biji cendana secara invitro. Penelitian ini dilakukan dengan menambahkan Pupuk Organik Cair (POC) pada medium Murashige and Skoog (MS). Hasil penelitian menunjukkan bahwa penambahan konsentrasi 2 mL POC nasa pada medium MS berdampak baik terhadap perkecambahan Cendana (Santalum album  L.) dengan konsentrasi 2 mL yang merupakan konsentrasi optimal.  Respon perkecambahan ditunjukan pada 14 hari setelah ditanam pada medium MS.
Kebutuhan Protein/Asam Amino dan Karbohidrat Bagi Udang-Udang Penaeid: Suatu Studi Literatur Rosyida, Eka
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (177.845 KB) | DOI: 10.24002/biota.v12i1.2531

Abstract

Suatu studi literatur dilakukan untuk menelaah kebutuhan protein/asam amino dan karbohidrat bagi udang-udang penaeid, suatu jenis udang yang bernilai ekonomis penting di Indonesia maupun di pasaran dunia. Salah satu hal penting dalam membudidayakan udang tersebut adalah berkaitan dengan kebutuhan nutrisi pada fase larva. Pemberian nutrisi yang buruk dianggap secara langsung maupun tidak langsung dapat menyebabkan timbulnya beberapa penyakit, kematian massal dan penurunan produksi. Kebutuhan protein udang penaeid mempunyai korelasi dengan aktivitas enzim proteolitik dan dipengaruhi oleh umur, ukuran dan tahapan/fase dari siklus hidup udang tersebut. Faktor kualitas dan tingkat energi dari sumber protein yang diberikan secara signifikan berpengaruh terhadap pertumbuhan udang, sedangkan disakarida dan polisakarida dianggap lebih efisien sebagai sumber karbohidrat untuk udang penaeid dibanding monosakarida.
Insecticidal Activity of Extracellular Protein of PRU8 Isolate against Tenebrio molitor Larvae Akhdiya, Alina; Pratiwi, Etty; Samudra, I Made
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (233.506 KB) | DOI: 10.24002/biota.v14i2.2684

Abstract

Sel ataupun supernatan bebas sel dari kultur cair isolat bakteri entomopatogen PRU8 memiliki toksisitas tinggi terhadap larva Tenebrio molitor. Bioesei protein ekstrasel kasar hasil pengendapan amonium sulfat menunjukkan bahwa toksisitas tertinggi terdapat pada protein yang diendapkan pada kejenuhan amonium sulfat 70%. Pemurnian protein menggunakan kolom Hi Prep 16/60 Sephacryl S-200 HR menghasilkan satu fraksi protein toksin. Mortalitas larva uji yang disuntik dengan 19,2 nanogram toksin murni mencapai 71%. Uji toksisitas menggunakan toksin murni menunjukkan bahwa toksin tersebut termasuk kelompok toksin tipe injeksi. Berdasarkan analisa SDS-PAGE toksin tersebut tersusun atas dua protein dengan berat molekul 116,25 dan 66,24 kDa
Pemanfaatan Oleoresin Jahe (Zingiber officinale) untuk Mengatasi Kelainan Antioksidan Intrasel Superoxide Dismutase (SOD) Hati Tikus Di Bawah Kondisi Stres Wresdiyati, Tutik; Astawan, Made; Adnyane, I Ketut Mudite; Prasetyawati, Renny Candra
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (266 KB) | DOI: 10.24002/biota.v10i2.2848

Abstract

This study was conducted to evaluate the activity of ginger oleoresin (Zingiber officinale) on the intracelluler antioxidant-superoxide dismutase (SOD) in the liver of rats under stress condition. A total of twuenty five Wistar rats were used for this study. They were divided into five groups ; (1)K (control), (2) S (stress), (3)O + S (oleoresin followed by stress), (4) S + O (stress followed by oleoresin and (5) O + S +O (oleoresin followed by stress then oleoresin). The dose of oleoresin is 60mg/Kg/BW/day for seven days. Stress condition was done by five days fasting and smimming for five minutes/day, while drinking water was provided ad libitum to all groups. The results showed that ginger oleoresin significantly decreased malonaldehyde (MDA) and elevated SOD activity. The immunohistochemical evaluation also showed that ginger oleoresin increased the content of copper,zinc-superoxide dismutase (Cu,Zn-SOD) in the liver of rats under stress condition. These effects were showed in the tissues of rats treated by ginger oleoresin before or after stress or combination of both.
Keanekaragaman Lumut di Kawasan Cagar Alam Dungus Iwul, Jasinga, Jawa Barat Windadri, Florentina Indah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (289.579 KB) | DOI: 10.24002/biota.v15i3.2596

Abstract

Exploration and collection of the mosses species in Dungus Iwul Nature Reserve were conducted. Thirty eight species including 26 genera and 11 families were recorded. Three species had categories as endemic species i.e Fissidens teysmannianus and Orthomnion javense as endemic species in Java and Hyophila javensis as endemic species in Malesia. Ctenidium lychnites was recorded also as new species record in Java.
Effect of AMF Inoculation on the Growth of Combretocarpus rotundatus (Miq) on a Peat Soil from Central Kalimantan (For Restoration Ex-Mega Rice Project Central Kalimantan) Burhanuddin, Burhanuddin; Kabirun, Siti; Radjagukguk, Bostang; Sumardi, Sumardi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (483.518 KB) | DOI: 10.24002/biota.v15i1.2649

Abstract

Diperlukan teknik silvikultur dengan memanfaatkan jamur mikoriza arbuskula (JMA) untuk memulihkan hutan rawa gambut yang terdegradasi. JMA berperan penting pada pertumbuhan dan ketahanan hidup pohon-pohonan. Penelitian ini bertujuan untuk mengetahui keberadaan dan bentuk dari JMA di hutan rawa gambut yang sudah terdegradasi pada blok C bekas pengembangan lahan gambut satu juta ha di Kelampangan Kalimantan Tengah. Kondisi lahan hanya ditumbuhi vegetasi jenis-jenis pioner. Contoh tanah diambil dibawah perakaran lima jenis tanaman pioner (Melastoma sp, Combretocarpus sp, Acacia sp, Cratoxylon sp, dan Nephrolepsis sp), kemudian contoh tanah dihitung jumlah spora dengan metode saringan basah. Jumlah spora terbanyak ada di bawah perakaran tanaman Melastoma sp, kemudian Acacia sp, dan Combretocarpus sp. Didapatkan tiga genus JMA: Glomus, Gigaspora, dan Acaulospora. Penelitian di rumah kaca bertujuan untuk mengetahui pengaruh inokulasi JMA jenis Glomus sp TD15, Glomus sp D32, dan Gigaspora sp pada semai perepat (C. rotundatus). Hasil penelitian menunjukkan JMA memberikan pengaruh pada parameter semai yang diukur. Jenis JMA Glomus sp TD15 meningkatkan pertumbuhan semai perepat yang ditunjukkan oleh parameter tinggi semai, diameter semai, dan jumlah daun per tanaman. Pada media gambut semai C. rotundatus sangat tergantung pada asosiasi JMA dalam meningkatkan pertumbuhan tanaman.
Konsentrasi dan Kualitas Spermatozoa Kucing Domestik (Felis catus) yang diambil dari Epididymis dan Ductus deferens setelah Preservasi pada Suhu 4oC Nazlie (Alm), Cutnya’ Shaliran; Supriatna, Iman; Agungpriyono, Srihadi; Boediono, Arief
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 1 (2006): February 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (272.306 KB) | DOI: 10.24002/biota.v11i1.2820

Abstract

The aim of this study was to evaluate the concentration, progressively motile, and percent live sperm in the various regions of epididymis and ductus deferens after preservation at 4oC. Epididymis and ductus deferens were collected from 21 epididymis and ductus deferens of domesticated cat (Felis catus) by castration. One testicle of pair (control testicle) was analyzed at the day of castration, while the other testicle of the pair was stored at 4oC up to 7 days. The sperm concentration, percentage of sperm motility and live sperm were examined daily until day-7 of preservation. The sperm concentration was higher (p<0.05) in cauda epididymis (23.99x106 sperm/ml) and ductus deferens (25.42x106 sperm/ml) than caput (11.51x106 sperm/ml) and corpus epididymis (14.82x106 sperm/ml). The percentage of sperm motility and live sperm decreased (p<0.05) during preservation period. However, the percentage of motile (11.33 to 16.00 %) and live (15.05 to 20.20 %) sperm could be found in preserved epididymis and ductus deferens up to day-7. These results show that motile and live sperm can be collected from cat’s epididymis and ductus deferens up to day 7 after preservation at 4oC.
Opini Terkini Tentang Dinding Sel Tanaman: Struktur, Biosintesis, dan Aplikasinya Yulia, Dina; Yusriana, Bintarti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.158 KB) | DOI: 10.24002/biota.v11i3.2548

Abstract

Plant cell walls are unique, complex, diverse and dynamic structures that change throughout the process of cell division, growth and differentiation. Plant cell walls are not only a single key determinant of overall plant form, but they also play an important role in sustaining human societies. Evidence of how cell walls are crucial for plant structure is that without cell walls, plants would be elastic piles of protoplasm, more like slime moulds than stately trees. Unlike animals, in which specialized skeletal system provides physical support, the strength, flexibility, texture and overall shape of higher plants depend on the cumulative properties of walls. Moreover, plants are composed of approximately 35-40 cell types that are distinguished from each other by the chemistry and organization of their walls. In human societies, cell walls directly affect the quality of most plant-based products, including paper and pulping, textile, food quality and texture, dietary fibre, malting and brewing and bioethanol production (Cosgrove, 2005; Farrokhi et al., 2006).The chemical structure of most wall components has been intensively studied and defined in detail, however, the enzymes responsible for cell wall biosynthesis and also its restructure remain poorly understood. However, currently, there has been real progress towards understanding the cell wall biosynthesis, using various emerging approaches, such as genetic and biochemical approaches (Farrokhi et al., 2006).This review will elaborate on recent advances in our knowledge of the structure cell walls of flowering plants, particularly the structure of primary cell walls. Moreover, this review will also explain the current knowledge towards understanding primary wall biosynthesis and also the future application of cell wall study in biotechnology.
Waktu Reproduksi Karang Lunak Sinularia flexibilis Quoy & Gaimard (Octocorallia: Alcyonacea) Di Pulau Barrang Lompo, Kepulauan Spermonde, Sulawesi Selatan Haris, Abdul
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 2 (2010): June 2010
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (296.4 KB) | DOI: 10.24002/biota.v15i2.2706

Abstract

Sinularia flexibilis is a soft coral that has an economical value due to its farmacological substance. The aim of this research was to find out the spawning time and behaviour of Sinularia flexibilis by visual observation in its natural habitat and in hatchery. The result of this research revealed that the reproduction period of the Sinularia flexibilis occurred during May to September in each lunar phase. However, it was found that during a new moon phase, the reproduction activity had the highest percentage of colony for every lunar phase. Spawning behavior of the Sinularia flexibilis was that a bunch of eggs and the sperm were separately spawned from the mouth by fast contraction in a short period of time.
Penghambatan Produksi Aflatoksin dari Aspergillus flavus oleh Kapang dan Khamir yang Diisolasi dari Ragi Tempe Purwijantiningsih, Ekawati; Dewanti-Hariyadi, Ratih; Nurwitri, C. C.; Istiana, Istiana (alm.)
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 3 (2005): October 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (192.716 KB) | DOI: 10.24002/biota.v10i3.2874

Abstract

This research was carried out to study the inhibition of aflatoxin production of Aspergillus flavus by moulds and yeasts isolated from ragi tempe. The result showed that moulds found in the ragi tempe consisted of Rhizopus oligosporus, R. oryzae, R. cohnii, Mucor rouxii, Rhizomucor pusillus, Aspergillus niger and miselia sterilia. Yeasts found in the samples were Candida sp1, Candida sp2 and Trichosporon sp. Twelve mould, and yeast isolates were chosen to determine their ability on inhibition of aflatoxin production. Aspergillus flavus alone or in association with each of these isolates were inoculated in Potato Dextrose Broth and analysed for aflatoxin production at day 10. All combinations yielded less aflatoxin than the single culture of A. flavus. All isolates tested could inhibit aflatoxin production. The highest inhibition was shown by Candida sp1 (99,96%).

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