cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
Brigitta Laksmi Paramita
Contact Email
brigitta.laksmi@uajy.ac.id
Phone
+6282329549978
Journal Mail Official
journal.biota@gmail.com
Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia
Location
Kota yogyakarta,
Daerah istimewa yogyakarta
INDONESIA
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati
Published by Universitas Atma Jaya Yogyakarta
ISSN : 25273221     EISSN : 2527323X     DOI : doi.org/10.24002/biota
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology
Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 1,193 Documents
 24   25   26   27   28 
Kajian Penanda Genetik Tarsius bancanus dan Tarsius spectrum dengan Sekuen D-Loop Parsial DNA Mitokondria Widayanti, Rini; Solihin, Dedi Duryadi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 3 (2007): October 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (307.5 KB) | DOI: 10.24002/biota.v12i3.2804

Abstract

The objective of this research was to study the specific genetic marker on D-loop region of Tarsius bancanus and Tarsius spectrum. The sequencing of PCR product using primer DLTARPROF on D-loop resulted in base sequence of 270 nts. Result of D-loop fragments sequencing was put on multiple alignment with other primates from Genbank with the aid of software Genetyc-Win Version 3.0 and Clustal W, and was analyzed using MEGA program version 3.1. The genetic distance was based on nucleotide D-loop, the smallest genetic distance was 0% and the biggest was 11.8% and the average was 2.3%. The phylogenetic tree using neighbor Joining Method based on some nucleotide sequence on D-loop region could not be used to differentiate between Tarsius bancanus and Tarsius spectrum.
Catatan Baru Persebaran Pinang Gajah (Nenga gajah J. Dransf.) (Short Communication) Witono, Joko R.; Sutrisno, Sutrisno; Astuti, Inggit Puji
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 3 (2004): October 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (161.807 KB) | DOI: 10.24002/biota.v9i3.2917

Abstract

Sumatra merupakan salah satu pulau besar di kawasan Malesia Barat yang memiliki keanekaragaman palem yang tinggi. Menurut Uhl & Dransfield (1987), di Sumatra terdapat 26 marga palem, salah satu diantaranya adalah Nenga. Nenga terdiri atas 5 jenis, yaitu Nenga gajah, N. pumila, N. banaensis, N. macrocarpa, dan N. grandiflora (Fernando, 1983). Kelima jenis tersebut tumbuh pada daerah hutan hujan tropika pada ketinggian antara 0-1.400 m dpl. Daerah persebarannya di Asia Tenggara mulai Thailand, Semenanjung Malaya, Sumatra, Jawa, dan Kalimantan. Satu-satunya jenis Nenga yang dinyatakan sebagai jenis endemik, sekaligus langka adalah Nenga gajah (WCMC, 1996) karena populasinya yang sangat rendah dan terancam oleh aktivitas perladangan liar dan perluasan kebun kopi rakyat.
Kinetika Adsorpsi Ion Besi (II) Oleh Biomassa Chaetoceros sp. Widyawati, Paini Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (190.293 KB) | DOI: 10.24002/biota.v11i3.2542

Abstract

This research was done to study the adsorption kinetic of ferrous ionic by Chaetoceros sp. Many variables influenced the adsorption capacity of absorbent on ferrous ionic including interaction time, ferrous ionic concentration and pH. Two absorbens were used in this study i.e. pure culture and natural spoiled product called Diatomite/ Diatomae earth / Diatomaceous earth / Diatomooze of Chaetoceros sp. biomass. The adsorption capacity by biomass was measured by atomic absorption spectrophotometry method (AAS). The result showed that the adsorption process of two absorbents happened very fast. The time needed to get maximal adsorption were 10 and 15 minutes respectively. Adsorption pattern of two biomass can be interpreted by Langmuir and Freundlich isoterm showing monolayer. The adsorption capacity of Diatomite was five times higher than that of the pure culture biomass because it was influenced by surface group charge and wide surface area of porous. Acidity degree (pH) of solution determined surface active group charge and solubility of iron (II). The increased pH value, the biomass adsorption capacity was added because surface active group had negative charge. The adsorption of biomass was maximal around pH 5 while for pH higher than 5, it wasn’t significantly increased because the iron (II) formed insoluble hydroxide compound.
Expression and Characterization of Recombinant Protein of J-SU pGEX either by Single or Double Cell Lysis Margawati, Endang Tri; Ridwan, Muhamad
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (201.705 KB) | DOI: 10.24002/biota.v14i3.2579

Abstract

Penelitian ini dimaksudkan untuk optimasi produk protein rekombinan Superficial Unit dari virus Jembrana (JSU) yang dieksperikan melalui pemecahan sel secara tunggal dan ganda dengan sistem pGEX dalam skala flask 100ml media kultur. Dua metode pemecahan sel yang digunakan yaitu Freeze and Thaw (FT) sebagai pemecahan tunggal dan gabungan FT dan Sonikasi sebagai pemecahan ganda. Sel inang (E. Coli pembawa konstruk JSU pGEX) ditumbuhkan dengan induksi IPTG pada 37oC dengan pengocok berkecepatan 200rpm sampai mencapai kepadatan sel 0,8. Sel atau pelet dikoleksi dengan sentrifugasi, pelet dipecah dengan 2 perlakuan pemecahan sel tunggal dan ganda. Hasil pemecahan sel disentrifugasi untuk dikoleksi peletnya sebagai inclusion body. Solubilisasi dilakukan terhadap inclusion body dengan solubilisasi buffer dan diperoleh substrat protein JSU kemudian dimurnikan melalui Gluthation sepharose 4B (500μl resin) dengan metode batch capture. Hasil karakterisasi dengan SDS PAGE dan Western Blotting menunjukkan ukuran protein JSU pGEX yang tepat yaitu 60kDa pada kedua sistem pemecahan sel. Namun demikian, pemecahan sel secara tunggal menghasilkan protein murni JSU pGEX lebih besar (0.812ng/ul) dibanding pemecahan sel secara ganda (0.486ng/ul). Dari penelitian ini dapat disimpulkan bahwa protein rekombinan JSU pGEX terekspresi lebih baik dengan metode pemecahan sel Freeze and Thaw.
Penelitian Mikrobiologi Menjadi Mudah dan Menarik Atmodjo, P. Kianto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (334.274 KB) | DOI: 10.24002/biota.v12i2.2670

Abstract

Mau tahu apakah suatu sabun betul-betul berperan sebagai antispetik? Mau tahu apakah suatu bahan makanan (kalengan) mengandung mikrobia atau tidak? Mau mengenal keanekaragaman mikrobia dari berbagai sumber lingkungan? Mau mempelajari teknik rekayasa mikrobia untuk dijadikan mikrobia baru? Semua itu dapat diperoleh dalam buku “Bensen’s Microbiological Applications”.
Kimia Organik Industri (Kajian Buku) Pranata, F. Sinung
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 1 (2005): February 2005
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (181.855 KB) | DOI: 10.24002/biota.v10i1.2838

Abstract

Perkembangan yang pesat di bidang industri kimia telah membuat Klaus Weissermel dan Hans-Jurgen Arpe membuat edisi baru dari buku Kimia Organik Industri. Buku ini merupakan revisi dari edisi pertama dan telah diterjemahkan ke dalam delapan bahasa. Pada tahun 1976, Weissermel dan Arpe menerbitkan buku ini pertama kali di Jerman. Buku tersebut mendapat sambutan positif dari para koleganya. Tidak lebih setahun kemudian edisi kedua telah diterbitkan dengan dilengkapi data terbaru dari berbagai industri kimia di dunia.
Karakterisasi Genetik Kambing Gembrong dari Karangasem Bali Menggunakan DNA Mikrosatelit Puja, I Ketut; Sulabda, I Nyoman
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (156.575 KB) | DOI: 10.24002/biota.v14i1.2632

Abstract

The present study was undertaken with primary objective to characterize of Gembrong goat breeds. It is essential to characterize the germplasm for intragenetic variability, which will help in planning for conservation strategy as well as genetic improvement. DNA Genome was isolated from hairs. Nine microsatellite were amflified by PCR. PCR product were run on 6% bis-Acrylamide gel in automated DNA sequencer. Flourescent signals from the dye-labeled microsatellite were detecting using STRand software. The result show that all markers (INRA005, INRA063, INRA023, ILSTS19, ILSTS87, SRCRSP8, MAF65, OarFCB20, and McM547) successfully amplified in Gembrong goat microsatellite loci. The number of alleles per locus ranged from 1 (INRA005) to 4 (ILSTS87). All the microsatellites investigated were found to be highly polymorphic, except INRA063. In total, 23 alleles were observed for the 9 microsatellite loci. The allele sizes ranged from 99 bp (OarFCB20) to 240bp (SRCRSP8). The study can be extended to include large many microsatellites in different chromosome location to validate the results.
Pengaruh Jarak Penyekat dalam Fotobioreaktor Tubular terhadap Pertumbuhan Kultur Mikroalga Ankistrodesmus convulutus Chrismadha, Tjandra; Rustiani, Tenni; Rosidah, Rosidah; Mardiati, Yayah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (337.514 KB) | DOI: 10.24002/biota.v12i1.2530

Abstract

A series of partition was constructed inside a vertical tubular photobioreactor to control the culture distribution in obtaining intermittent exposure to light area in the surface of the tubular column. A microalga, Ankistrodesmus convulutus, was grown in a batch mode in the reactor with PHM medium, light sources of fluorescent lamp 3 x 40 watt (I = 5.500 lux on the surface of the tubular) and room temperature of 27 – 310C. The experiment was carried out to study the influence of partition density which was constructed at varied distance, which were 1, 2 and 3 inch on the growth and biochemical composition of the alga. A vertical tubular photobioreactor without partition was applied as the control. The partition density did not significantly affect the optical density achievement of the culture, but it significantly affected the biomass and chlorophyll concentration, as well as the protein/carbohydrate ratio of the culture. It is believed that the intermittent exposure of algal cell to the light area leads to sincronization of the light and dark reaction of the photosynthesis, as well as to vapor the catalitic proccesses to develop the functional cell structure.
Distribusi Southeast Asia Ovalocytosis (SAO) pada Berbagai Populasi Masyarakat di Kepulauan Sunda Kecil Lanni, Fransiska
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (203.422 KB) | DOI: 10.24002/biota.v9i2.2897

Abstract

The presence of some genetic markers related to malaria as hemoglobinopathies, enzymophaties and ovalocytosis in populations of Lesser Sunda Islands  are presumed to be quite high, since this archipelago is mostly malaria endemic. To elucidate the problem, a series of observations had been done among  579 blood samples which were collected from five different populations (Balinese, Sasak, Sumbawanese, Sumbanese,  and Alorese) representing inhibitant of Lesser Sunda Islands. In this paper we present the distribution of Southeast Asia Ovalocytosis (SAO) which was detected  by microscopic examination of the red blood cell morphology. Among these, 38 subjects were suspected as SAO whereas the incidence ranges from 2.2% in Balinese to 15.7% in Alorese.  It was predicted that the high incidence of SAO in Alorese might be associated with malaria selection to compensate the absence of hemoglobinophaties in this population.
Pengaruh Suhu Annealing dan Konsentrasi MgCl2 terhadap Spesifisitas Amplikon dengan Primer CSSM018 Indriawati, Indriawati; Margawati, Endang T.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (174.26 KB) | DOI: 10.24002/biota.v14i2.2683

Abstract

Polymerase chain reaction (PCR) is a technique of DNA amplification which its success depends on the wider range of several factors, e.g., the annealing temperatures and MgCl2 concentrations. The aim of this study was to determine the effect of annealing temperatures and MgCl2 concentrations on the amplicon specificity of CSSM018 primer. Annealing temperatures of 50oC to 60oC were used in this study while MgCl2 concentrations were in the range of 1.0mM to 2.5 mM. DNA genome of Garut sheep was used as DNA template. PCR products were visualized by a method of 8% ND PAGE. The findings showed that the annealing temperature of 60oC presented the most specific band of the CSSM018 primer at the right size of 116-134bp. Annealing temperatures that were less than 60oC showed the weaker and non-specific bands. A concentration of 1.25 mM MgCl2 showed the best band of the CSSM018 primer with size of 116-134bp. While concentrations of 1.5; 1.75; 2.0 and 2.5 mM MgCl2 showed sharper with some bands seemed noisy, those indicated non-specific products. This study suggests that annealing temperature of 60oC and MgCl2 concentration of 1.25 mM resulted specific amplicon for CSSM018 primer.

Page 26 of 120 | Total Record : 1193

 24   25   26   27   28 

Filter by Year

2003 2026


Reset
Filter By Issues
All Issue Vol 11, No 1 (2026): February 2026 Vol 10, No 3 (2025): October 2025 Vol 10, No 2 (2025): June 2025 Vol 10, No 1 (2025): February 2025 Vol 9, No 3 (2024): October 2024 Vol 9, No 2 (2024): June 2024 Vol 9, No 1 (2024): February 2024 Vol 8, No 3 (2023): October 2023 Vol 8, No 2 (2023): June 2023 Vol 8, No 1 (2023): February 2023 Vol 7, No 3 (2022): October 2022 Vol 7, No 2 (2022): June 2022 Vol 7, No 1 (2022): February 2022 Vol 6, No 3 (2021): October 2021 Vol 6, No 2 (2021): June 2021 Vol 6, No 1 (2021): February 2021 Vol 5, No 3 (2020): October 2020 Vol 5, No 2 (2020): June 2020 Vol 5, No 1 (2020): February 2020 Vol 4, No 3 (2019): October 2019 Vol 4, No 2 (2019): June 2019 Vol 4, No 1 (2019): February 2019 Vol 4, No 1 (2019): February 2019 Vol 3, No 3 (2018): October 2018 Vol 3, No 2 (2018): June 2018 Vol 3, No 1 (2018): February 2018 Vol 3, No 1 (2018): February 2018 Vol 2, No 3 (2017): October 2017 Vol 2, No 2 (2017): June 2017 Vol 2, No 1 (2017): February 2017 Vol 2, No 1 (2017): February 2017 Vol 1, No 3 (2016): October 2016 Vol 1, No 2 (2016): June 2016 Vol 1, No 1 (2016): February 2016 Vol 1, No 1 (2016): February 2016 Vol 19, No 1 (2014): February 2014 Biota Volume 19 Nomor 1 Tahun 2014 Biota Volume 13 Nomor 2 Tahun 2014 Vol 18, No 2 (2013): June 2013 Vol 18, No 1 (2013): February 2013 Biota Volume 18 Nomor 1 Tahun 2013 Vol 17, No 3 (2012): October 2012 Vol 17, No 2 (2012): June 2012 Vol 17, No 1 (2012): February 2012 BIOTA Volume 17 Nomor 3 Tahun 2012 Vol 16, No 2 (2011): June 2011 Vol 16, No 2 (2011): June 2011 Vol 16, No 1 (2011): February 2011 Vol 16, No 1 (2011): February 2011 Vol 15, No 3 (2010): October 2010 Vol 15, No 2 (2010): June 2010 Vol 15, No 1 (2010): February 2010 Vol 14, No 3 (2009): October 2009 Vol 14, No 2 (2009): June 2009 Vol 14, No 1 (2009): February 2009 Vol 13, No 3 (2008): October 2008 Vol 13, No 2 (2008): June 2008 Vol 13, No 1 (2008): February 2008 Vol 12, No 3 (2007): October 2007 Vol 12, No 2 (2007): June 2007 Vol 12, No 1 (2007): February 2007 Vol 11, No 3 (2006): October 2006 Vol 11, No 2 (2006): June 2006 Vol 11, No 1 (2006): February 2006 Vol 10, No 3 (2005): October 2005 Vol 10, No 2 (2005): June 2005 Vol 10, No 1 (2005): February 2005 Vol 9, No 3 (2004): October 2004 Vol 9, No 2 (2004): June 2004 Vol 9, No 1 (2004): February 2004 Vol 8, No 3 (2003): October 2003 Vol 8, No 2 (2003): June 2003 Vol 8, No 1 (2003): February 2003 More Issue