Garuda - Garba Rujukan Digital

Article Per Year (5 Year)

All

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
Website

Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 1,193 Documents

36 37 38 39 40

Uji Patogenisitas Isolat Bakteri Indigenous (Bacillus thuringiensis) terhadap Serangga Hama Kubis (Crocidolomia binotalis Zell) Salaki, Christina L.; Situmorang, Jesmandt; Sembiring, Langkah; Handayani, Niken
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Pathogenicity of 34 indigenous B. thuringiensis isolates against C. binotalis were determined. The pathogenicity test was conducted by using leaf dipped method with various spore concentrations. Third instar larvae of C. binotalis were used as insect test. Mortality data of test larvae were used to determine the pathogenicity of the isolates in terms of 72 hours LC50 by using probit analysis. The results of experiments showed YPPA 1. was the most pathogenic isolate, producing 72 hours LC50 = 9.5 x 103 spore.ml-1 with LT50 (1.5 x 107 spore.ml-1) of 24.6 hours while the ACH 2.3 was found to be the least pathogenic isolate with 72 hours LC50 = 2.3 x 106 spore.ml-1 and LT50 (1.5 x 107 spoore.ml-1) of 40.7 hours. The shortest LT50 (1.5 x 107 spore.ml-1 was found to be 18.2 hours produced by TUS.1 with 72 hours LC50 = 3.9 x 105 spore.ml-1 whereas the longest LT50 (1.5 x 107 spore.ml-1) was found tobe 83.2 hours produced by the SLK 4.1 with 72 hours LC50 = 3.1 x 104 spore.ml-1. Therefore, it can be concluded that both YPPA.1 and TUS.1 isolates are potential candidate to be developed for biological control agent.

Periode Pemijahan Spons Aaptos aaptos (Porifera: Demospongia) di Perairan Pulau Pari, Kepulauan Seribu, DKI Jakarta Kawaroe, Mujizat; Soedharma, Dedi; Siregar, Rahmadsyah Deny
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 2 (2008): June 2008
Publisher : Universitas Atma Jaya Yogyakarta

The purpose of this research was to study spawning period of sponge Aaptos aaptosat Pari Island, Thousand Islands, DKI Jakarta. The result showed that spawningtime occurred at 17.00-18.00, and within that time the sponge closed their osculumto throw out the zygote leaving the body faster. The duration of spawning rangefrom 4 minutes to 41 minutes. Based on the lunar periode, spawning for spongeAaptos aaptos started from early new moon till few days after full moon. Spawningmostly took place during spring tides. Based on the PCA analysis, it was found thatwater temperature and pressure had strong correlation with spawning time.

Penurunan Glukosa dan Perubahan Profil Lipida Serum Tikus Sprague- Dawley Hiperglikemia-Hiperkolesterolemia Akibat Asupan Sorbitol-Oleat Poliester (SOPE) Murdiati, Agnes; Wuryastuty, Hastari; Marsono, Y.; Harmayani, Eni
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Disorder of carbohydrate metabolism in the Diabetes Mellitus patients resulted in increasing of cholesterol synthesis. This condition increases the risk of heart attack or coronary heart disease, therefore it is recommended to lower fat intake. One of the alternatif to lower fat intake is by replacing fat with fat replacer in the diet. The aim of the present study is to evaluate the effect of replacing 75% of fat with SOPE on the glucose and lipid profile in diabetic-hipercholesterolemic rats. Eihgteen Sprague Dawley rats were devided into 2 groups of 9 rats. They were fed with hipercholesterol diet and were diabetic induced with alloxan IM injection. One group was fed with 75% SOPE substitute the fat in the standard diet and the other group was fed with standard diet. The result indicates that the oil substitution of standard diet of Sprague Dawley rats for sorbitol-oleic polyester as much as 75% gives an advantage to rats which suffer hiperglikemic-hypercholesterolemic because it decreased the glucose, total cholesterol, LDL cholesterol, and LDL/HDL cholesterol ratio, and did not decrease the HDL cholesterol, but increased triglyceride serum.

Perilaku Makan Badak Sumatera di Suaka Rhino Sumatera, Taman Nasional Way Kambas, Lampung Suharto, Ignatius; Rustiati, Elly Lestari; Adi, Marcelus
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

Research on the feeding behaviour, feeding plants and the way to access the plants has been conducted on Sumateran rhinos at and in collaboraton with Sumatran Rhino Sanctuary, Way Kambas National Park, Lampung, by scanning and rapid assessment methods. Feeding activity is done during day time, especially in the morning (05.00-10.00) and in the afternoon (14.00-18.00). Plants consumed include saplings (42%), followed by trees (30%), seedlings (20%) and liana (8%). Cutting (86%) is the most common way to access the food sources, followed by pulling (9%), pushing by its head and holding in between front legs (4%) and twisting by its horn (1%).

Diversity and Abundance of Ant (Formicidae) Functional Groups in Range of Land Use Systems in Jambi, Sumatra Susilo, F. X.; Hazairin, M.; Hardiwinoto, Suryo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

Kerusakan hutan tropika basah dapat menimbulkan dampak lingkungan berupa penurunan keanekaragaman hayati dan terganggunya fungsi serta stabilitas ekosistem. Tujuan dari penelitian ini adalah untuk mendapatkan bukti apakah perubahan hutan tropika basah menjadi bentuk penggunaan lahan lain berakibat pada penurunan keragaman dan kelimpahan semut, dan untuk menunjukkan apakah semut dapat dijadikan sebagai bio-indikator perubahan sistem penggunaan lahan (SPL). Penelitian ini dilakukan di Jambi Sumatra pada akhir musim penghujan (Meiâˆ’Juni) 2004 pada berbagai taraf intensifikasi SPL. Inventarisasi dan koleksi semut dilakukan dengan menggunakan metode â€œWinklerâ€ pada enam SPL, yaitu: hutan primer, hutan sekunder, perkebunan karet, perkebunan kelapa sawit, ladang ketela pohon, dan padang alang-alang. Hasil penelitian menunjukkan bahwa ditemukan 50 genus semut pada 6 SPL tersebut. SPL tidak berpengaruh secara nyata terhadap keragaman dan kelimpahan seluruh semut tetapi berpengaruh secara nyata terhadap kelompok semut pesaba dan semut predator. Keragaman jenis dan kelimpahan yang tinggi dari semut predator ditemukan pada SPL hutan primer dan hutan sekunder. Keragaman jenis dan kelimpahan menjadi rendah apabila SPL hutan diubah menjadi SPL ladang ketela pohon. Kelimpahan semut pesaba tertinggi diketemukan pada SPL perkebunan kelapa sawit. Data yang diperoleh menunjukkan bahwa semut dapat digunakan sebagai bio-indikator dalam perubahan SPL di kawasan Jambi, Sumatra.

Enkapsulasi dan Regenerasi Kalus Embriogenik Mangga (Mangifera indica L.) Kultivar Bapang dan Gadung 21 Ermayanti, Tri Muji; Nugroho, Robertus; Hamim, Hamim
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

The mother plant and genetic variability of Indonesian mango need to be conserved. Encapsulation is one of in vitro conservation used for many plant species. The aim of this research was to study the regeneration of encapsulated mango cultivar Bapang and Gadung 21 embryogenic callus after storage at -14, 3âˆ’5, and 26âˆ’27oC for 0, 1, 2, 4, and 8 weeks. Embryogenenic callus was treated with 3% Na-alginate (in liquid 3M), then it was dropped into 100 mM CaCl2. Encapsulated callus beads were dehidrated and stored at -14, 3âˆ’5, and 26âˆ’27oC for 0, 1, 2, 4, and 8 weeks. After storage, the callus was cultured in 3M medium containing 2,4-D at 0, 1, and 2 mg/l for the regeneration. The results showed that after 8-week storage, callus of Bapang culticar was more viable (67.3%) and resulted more number of somatic embryos (191.6%) than Gadung 21 cultivar. The callus which was cultured in 3M medium without addition of 2,4-D was more viable (20.9%) and had more number of somatic embryos (1062.5%) than that which was cultured on medium containing 2,4-D. After 2-week storage, callus had viability of 7.6%. No storage callus formed more somatic embryos than storage callus. Storage at 26âˆ’27oC gave higher viability as well as higher number of somatic embryo than stored at -14 and 3âˆ’5 oC. The callus did not regenerate into shoots after storage at -14oC. Embryogenic callus could be stored at 3âˆ’5 and 26âˆ’27oC for 4 weeks.

Keragaman dan Pemanfaatan Tumbuhan Berenuk (Cresentia cujete L) di Daerah Istimewa Yogyakarta Atmodjo, Kianto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 4, No 3 (2019): October 2019
Publisher : Universitas Atma Jaya Yogyakarta

Berenuk (Crescentia cujete Linn, suku Bignoniaceae ) merupakan tumbuhan yang sebarannyaÂ di daerah tropis.Â Arango-Ullao (2009) menemukan dan membagi 8 ragam buah tumbuhan iniÂ di daerah Colombia. Di FilipinaÂ berenuk dikenal sebagai salah satu tumbuhan obat ajaibÂ Semua bagian tumbuhan iniÂ dapat dimanfaatkanÂ dari sebagai obat, perabot rumah tangga dan hiasan. Di Indonesia, berenuk banyak dijumpai, namun keberadaannya terancam punah karena masyarkat tidak mengetahui manfaatnya, bahkan dianggap berbahaya sebagai racun dan akhirnya ditebangi. Penelitian ini dilakukan untuk mengetahui keberadaan, keberagaman dan pemanfaatan berenuk oleh masyarakat Indonesia yang diawali dari Daerah istimewa Yogyakarta. Metode yang dilakukan dengan cara wawancara dengan tokoh masyarakat dan mendatangi lokasi yang dianggap ada tumbuhan ini. Bila tumbuhan ini ditemukan didata morfologis buahnya dan dicari informasiÂ pemanfaatannya dari orang disekitar tumbuhan ini berada. Penelitian ini dilakukan sejak bulan SeptemberÂ 2018 sampai dengan april 2019 di lakukan di daerah Sleman,Kulon Progo, Bantul, Gunungkidul dan Kota madya Yogyakarta. Hasil yang diperoleh adalah berenuk lebih dikenal dengan nama daerah maja (pahit) dijumpai di semua Kabupaten dan Kotamadya di daerah Istimewa Yogyakarta dalam kondisi terancam punah. Ada 4 macam variasi tumbuhan berdasarkan bentuk buahnya yaitu lonjong, bundar,danÂ sphaeris, serta seperti ginjal.Ukuran buah mancapai 3,5 kg yang terbesar dengan keliling sekitar 30 cm. Tumbuhan buah lonjong hanya dijumpai di daerah kota madya Yogyakarta, 5 pohon. Masyarakat daerah kulon progo memanfaatkan sebagai pakan ternak, di Bantul untuk minuman fermentasi, di Sleman untuk pestisida dan pupuk. UsahaÂ Pengenalan pengolahan buah berenuk dan merasakan manfaatnya sebagai obat sakit perut, asma, masuk angi dan gula di daerah Sleman telah dilakukan. Usaha ini berdampak dan telah mendorong masayarakat menanam dan mencegah penebangan berenuk.

Molecular Identification of Genes Involved in Magnetosome Synthesis in Magnetospirillum magneticum AMB-1 Wahyudi, Aris Tri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Satu mutan Magnetospirillum magneticum AMB-1 yang tidak bersifat magnetik, yang didesain NMA41, dikonstruksi melalui mutagenesis dengan transposon Mini-Tn5Km1 untuk mengidentifikasi gen yang terlibat dalam sintesis magnetosom. Mutagenesis dengan transposon dilakukan melalui konjugasi antara M. magneticum AMB-1 dan Escherichia coli S17-1 (ï¬ pir) yang membawa plasmid pUTmimi-Tn5Km1. Frekuensi transkonjugasi tertinggi berkisar 1.8 x 10-7 sel per resipien. NMA41 tidak respon terhadap bidang magnet dan kehilangan kemampuan dalam mensintesis magnetosom. Sekuens DNA/gen yang disisipi oleh transposon (dinamakan DNA pengapit) diisolasi dengan PCR yang dibalik (inverse PCR) dan diklon ke dalam plasmid pCR2.1. Penyejajaran sekuen DNA dari DNA pengapit terhadap sekuens DNA genom lengkap AMB-1 dapat mengidentifikasi sebuah kerangka baca terbuka (open reading frame, ORF2) dalam suatu operon yang terdiri dari 4 gen. Sekuen asam amino yang dideduksi dari ORF2 menunjukkan homologinya dengan protein domain GGDEF dari Magnetospirillum magnetotacticum MS-1 (identik 90%; kemiripan 95%) yang mempunyai fungsi dalam mekanisme transduksi sinyal. Gen atau operon ini diduga berfungsi selama proses sintesis magnetosom pada M. magneticum AMB-1.

Seleksi, Karakterisasi dan Identifikasi Bakteri Pendegradasi 2-(thiocyanomethylthio) benzothiazole (TCMTB) Sembiring, Langkah; Susilawati, Lela; Suhartanti, Dwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

The objective of this research was to investigate the capabilities of bacteria isolated from industrial tanning waste to degrade TCMTB. The bacteria was initialy screened, based on their tolerance to various concentration of TCMTB using paper disk method. Then, those strains were further analyzed in terms of their ability to produce ammonia (NH4+) and sulphate (SO42-). Degradation activity was measured based on remaining residue of TCMTB analyzed using HPLC. The superior strain that showed the highest activity in degradation of TCMTB then were characterized and identified based on phenotypic and 16S rDNA sequence analysis. The result of the experiments showed that four selected strains among seven were choosen based on their high tolerance to various concentration of TCMTB, namely PK1, PK2, PK4 and PK6. All four strains showed the ability to produce ammonia and sulphate but three of which, namely PK2, PK4 and PK6 showed the high capability to degrade TCMTB. One particular strain (PK2) was observed to degrade TCMTB 40.8% within 7 days, but the others were less than 30%. Based on the phenotypic characteristics and 16S rDNA sequence analysis, the best strains (PK2) was identified to be member of genus Pseudomonas.

Uji Antimikroba Fraksi Ekstrak Metanol, Etil Asetat dan n-Heksana Daun Tabar-Tabar (Costus speciosus) dan Toksisitasnya Terhadap Larva Udang Suryanto, Dwi; Kelana, Tata B; Wahyuni, Sri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

A Study on antimicrobial examination of fractions of methanol, ethyl-acetate, and n-hexane extract of tabar-tabar (Costus speciosus) leaf to bacteria (Bacillus sp., Staphylococcus aureus, Escherichia coli, and Serratia marcescens) and yeast (Candida albicans), and their toxicity to brine-shrimp (Artemia salina) larvae has been done. Examination of antimicrobial activities to the microbes were done by agar diffusion method, while test for toxicity was done by exposing 2 days-old larvae to certain concentration of the extract. Preliminary tests on phytochemical compounds in leaf were done by Mayerâ€™s, FeCl3, Mg-HCl, foam, and 10% NaOH in methanol tests. The results showed that methanol leaf extract of tabar-tabar in general inhibited more in the growth of gram-positive Bacillus sp. and S. aureus rather than to other tested microbes. Compared to methanol and ethyl-acetate fraction, n-hexane fraction of the leaf inhibited more Bacillus sp. and E. coli. Interestingly, C. albicans was highly inhibited by n-hexane fractions. LC50 of methanol extract, ethyl-acetate, and n-hexane fraction, were 45.53, 478.37, and 824.20 ppm, respectively. Preliminary test on phytochemical compounds showed saponin was only detected in fractions of methanol and ethyl acetate extracts, steroid was detected in fraction of methanol, while triterpenoid was detected in fraction of n-hexane extract. Phenolic was detected in all extract fractions, while flavonoid, kumarin, and alkaloid on the other hand were not detected.

Page 38 of 120 | Total Record : 1193

36 37 38 39 40

Filter by Year

2003 2026

Filter By Issues

All Issue Vol 11, No 1 (2026): February 2026 Vol 10, No 3 (2025): October 2025 Vol 10, No 2 (2025): June 2025 Vol 10, No 1 (2025): February 2025 Vol 9, No 3 (2024): October 2024 Vol 9, No 2 (2024): June 2024 Vol 9, No 1 (2024): February 2024 Vol 8, No 3 (2023): October 2023 Vol 8, No 2 (2023): June 2023 Vol 8, No 1 (2023): February 2023 Vol 7, No 3 (2022): October 2022 Vol 7, No 2 (2022): June 2022 Vol 7, No 1 (2022): February 2022 Vol 6, No 3 (2021): October 2021 Vol 6, No 2 (2021): June 2021 Vol 6, No 1 (2021): February 2021 Vol 5, No 3 (2020): October 2020 Vol 5, No 2 (2020): June 2020 Vol 5, No 1 (2020): February 2020 Vol 4, No 3 (2019): October 2019 Vol 4, No 2 (2019): June 2019 Vol 4, No 1 (2019): February 2019 Vol 4, No 1 (2019): February 2019 Vol 3, No 3 (2018): October 2018 Vol 3, No 2 (2018): June 2018 Vol 3, No 1 (2018): February 2018 Vol 3, No 1 (2018): February 2018 Vol 2, No 3 (2017): October 2017 Vol 2, No 2 (2017): June 2017 Vol 2, No 1 (2017): February 2017 Vol 2, No 1 (2017): February 2017 Vol 1, No 3 (2016): October 2016 Vol 1, No 2 (2016): June 2016 Vol 1, No 1 (2016): February 2016 Vol 1, No 1 (2016): February 2016 Vol 19, No 1 (2014): February 2014 Biota Volume 19 Nomor 1 Tahun 2014 Biota Volume 13 Nomor 2 Tahun 2014 Vol 18, No 2 (2013): June 2013 Vol 18, No 1 (2013): February 2013 Biota Volume 18 Nomor 1 Tahun 2013 Vol 17, No 3 (2012): October 2012 Vol 17, No 2 (2012): June 2012 Vol 17, No 1 (2012): February 2012 BIOTA Volume 17 Nomor 3 Tahun 2012 Vol 16, No 2 (2011): June 2011 Vol 16, No 2 (2011): June 2011 Vol 16, No 1 (2011): February 2011 Vol 16, No 1 (2011): February 2011 Vol 15, No 3 (2010): October 2010 Vol 15, No 2 (2010): June 2010 Vol 15, No 1 (2010): February 2010 Vol 14, No 3 (2009): October 2009 Vol 14, No 2 (2009): June 2009 Vol 14, No 1 (2009): February 2009 Vol 13, No 3 (2008): October 2008 Vol 13, No 2 (2008): June 2008 Vol 13, No 1 (2008): February 2008 Vol 12, No 3 (2007): October 2007 Vol 12, No 2 (2007): June 2007 Vol 12, No 1 (2007): February 2007 Vol 11, No 3 (2006): October 2006 Vol 11, No 2 (2006): June 2006 Vol 11, No 1 (2006): February 2006 Vol 10, No 3 (2005): October 2005 Vol 10, No 2 (2005): June 2005 Vol 10, No 1 (2005): February 2005 Vol 9, No 3 (2004): October 2004 Vol 9, No 2 (2004): June 2004 Vol 9, No 1 (2004): February 2004 Vol 8, No 3 (2003): October 2003 Vol 8, No 2 (2003): June 2003 Vol 8, No 1 (2003): February 2003 More Issue

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA