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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 1,193 Documents

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Komposisi Guild dan Lebar Relung Burung Strata Bawah di Sipisang, Sumatera Barat Novarino, Wilson; Mardiastuti, Ani; Prasetyo, Lilik B.; Widjakusuma, Reviany; Mulyani, Yeni A.; Kobayashi, Hiroshi; Salsabila, Anas; Jarulis, Jarulis; Janra, M. Nazri
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 13, No 3 (2008): October 2008
Publisher : Universitas Atma Jaya Yogyakarta

Guild composition and niche breadth are important point on avian studies. This paper describes the guild composition and niche breadth of understorey bird in Sipisang, West Sumatra. The study was conducted since May 2002 until October 2004 for approximately 10 days each month (totally 284 days or 51.120 net.hours). Fifteen mist nets were operated on ground level separately on three locations, which made 60 m line each. Mist nets were operated from 6.00 AM until 18.00 PM, and checked every two hours. The captured birds were identified, ringed, measured, weighted, photographed and released. In total 1061 individuals were captured during the study; those birds belong to 103 Species and 28 families. Based on guild, study area was dominated by insectivore-frugivore birds (IF) both in species and individual level. Meanwhile terrestrial frugivorous (TF) became the lowest one. Insectivore-frugivore also recorded has the broadest niche breadth. The bird communities dominated by small birds, which have weight lower than 30 g.

Observations on Natural Foods and Nutrition Content of Critically Endangered Turtle (Leucocephalon yuwonoi) in Central Sulawesi Riyanto, Awal; Wirdateti, Wirdateti; Soemarno, Suprayogo
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Kura-kura Sulawesi (Leucocephalon yuwonoi) adalah satu dari dua jenis kura-kura endemik Sulawesi dengan penyebaran yang terbatas. Sejauh ini data natural historinya sangat minim, padahal sangat dibutuhkan dalam upaya penangkaran satwa yang berstatus kritis IUCN ini. Penelitian ini bertujuan untuk mengungkap tentang pakan di alam beserta kandungan nutrisinya. Survei lapangan dilakukan di kawasan Bangkir dari tanggal 23 Mei sampai 5 Juni 2004 dan di Moutong dari tanggal 11 sampai 19 Oktober 2004. Data pakan di alam diperoleh dari analisis sampel feces dan hasil wawancara kepada para pemburu kura-kura beserta pengumpul. Kepastian nama jenis pakan diperoleh dengan melakukan identifikasi sampel tumbuhan pakan di Herbarium Bogoriense. Data kandungan nutrisi diperoleh melalui analisis proksimat sampel pakan yang terkoleksi selama survei. Hasil penelitian ini menunjukkan bahwa terdapat 32 jenis tumbuhan yang jadi sumber pakan di alam, variasi kandungan nutrisi yang cukup besar, kandungan lemak rendah berkisar antara 0.74 - 8.33% (2.41+2.03) dari berat kering. Berdasarkan dominansi, keberadaan di habitat (multiple season) dan tingginya kandungan energi diduga kuat bahwa Colocasia esculenta, Limnocharis flava and Ipomoea aquatica merupakan pakan utama di alam.

Karakterisasi Î±-Amilase dari Aspergillus versicolor 3a1 yang Diproduksi pada Media Limbah Cair Tapioka Aini, Fitratul; Mubarik, Nisa Rachmania; Manaf, Lisdar A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

The aim of this experiment was to characterize A. versicolor 3a1 Î±-amylase produced on cassava liquid waste media. Two types of media, base and combination media, were used as a comparison. Cassava liquid waste media contains 1% cassava starch, 1% yeast extract, 0.13% KH2PO4, and 0.05% MgSO4 diluted in cassava liquid waste. Base media contains same composition but using aquadest as a solvent, and combination media using mixture of aquadest and cassava liquid waste. A. versicolor 3a1 Î±-amylase showed its maximum specific activity in cassava liquid waste, base, and combination media after 3, 7, and 4 days incubation, respectively. Crude extract of Î±-amylase fromA. versicolor 3a1 was precipitated in 20-80% (w/v) ammonium sulphate. Precipitation of A. versicolor 3a1 Î±-amylase with 70% (w/v) ammonium sulphate on cassava liquidwaste, 60% on base media, and 60% on combination media will increase its specific activity 16.6, 4.28, and 5.65 times, respectively, compared to the specific activities ofcrude before precipitation. Î±-Amylase crude extract from A. versicolor 3a1 from all media showed its highest specific activity at 70oC and pH 5.0, and addition of FeSO4 increased the specific activity. Precipitated A. versicolor 3a1 Î±-amylase from all media showed its highest specific activity at 70oC and pH 6.0. Addition of FeSO4 precipitated 3a1 Î±-amylase from base and combination media will increase its specific activity, while MgSO4 will increase its specific activity in cassava liquid waste media. Thermostability assay revealed that the crude and the precipitated 3a1 Î±-amylase were relatively stable at 70oC up to 180 minutes incubation, except for precipitated3a1 ï¡-amylase on cassava waste media. Crude Î±-amylase 3a1 was relatively stable at pH 5-9 up to 1 hour incubation with wide pH ranges, while the precipitated with narrow pH ranges.

Uji Aktivitas Antibakteri dan Sitotoksik Ekstrak Metanol Aglaia silvestris (M.Roemer) Merr. Praptiwi, Praptiwi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

The antibacterial and sitotoxic activities of ganggo (Aglaia silvestris (M.Roemer) Merr. methanol extract were determined in this study. Antibacterial in-vitro test of ganggo methanol extract was exposed to Gram positive (Staphylococcus aureus ATCC 25923, Streptococcus agalactiae ATCC 8190, and Streptococcus sp.) and Gram negative (Salmonella typhii NCTC 786 E, Eschericia coli ATCC 25922, and Pseudomonas pseudomallei ATCC 15682) bacteria. The extract concentrations were 50, 25 and 12.5%, and done in triplicate. The growth inhibition area of extract was compared to those of standard antibiotic (10 unit ampicilin). Cytotoxic test of ganggo extract was done utilizing Brine Shrimp Lethality Test (BST) with Artemia salina.The result showed that growth inhibition area of 12.5% ganggo methanol extract to P. pseudomallei (19 mm) was wider than that of 10 unit ampicilin (0 mm). It showed that P. pseudomallei was sensitive to ganggo methanol extract. The result of BST showed that LC50 of ganggo extract was 345.44 ppm. It was concluded that ganggo methanol extract had antibacterial effects on some bacteria isolates and had cytotoxic effects with LC50 345.44 ppm.

PENEMPELAN LARVA SIPUT LAUT (HALIOTIS VARIA) : SUATU PERCOBAAN DI LABORATORIUM Ompi, Medy; Kawung, Nickson; Calvyn, Calvyn; Sondak, F. A.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Settlement of abalone (Haliotis varia) focusing on substrate quality and larval behavior were studied. Four types of substrate, shell and mucous, algae Gracilaria sp and Halimeda sp, and plastic petri-dish were used to test whether the larva might respond and settle on those substrates. At the same time, the mortality of larvae was also determined during the settlement. Without petri-dish, substrate of shell and mucous, algae, as well as polyvinyl chloride (PVC) plate were tested to find out substrate preferences when settlements occured. The result showed that variation of substrata, shell and mucous, algae and plastic ?petri-dish? possessed a stimuli responded by larva to settle. Shell and mucous as well as substrate algae were attractive among of substrata, since high of settlement on it were recorded. High larval mortality on shell and mucous as well as on substrata of algae were recorded. Substrate of shell and mucous and alga were favorable to substrata when settling. Several factors affecting settlement of larval abalone were discussed.

INVENTARISASI SPESIES MANGROVE DI TELUK KERTASARI, SUMBAWA BARAT Jupri, Ahmad
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 3 (2006): October 2006
Publisher : Universitas Atma Jaya Yogyakarta

Hutan mangrove atau bakau merupakan salah satu bentuk komunitas yang ada di daerah pasang surut dan khas yang mendukung kehidupan biota lain. Hock and Sasekumar (1979) melaporkan adanya primata yang hidup di hutan mangrove. Primata tersebut adalah monyet ekor panjang (Macaca fascicularis) dan lutung (Presbytis cristata). Selain itu, jenis-jenis burung, kepiting (Aratus pisonii, Erickson et al., 2004), semut (Oecophylla smaragdina, Offenberg et al., 2004). Selain itu, hutan mangrove ini sangat penting sebagai tempat berbiaknya jenis-jenis invertebrate dan ikan.

KUALITAS, KEMAMPUAN IMPLANTASI DAN VIABILITAS IN-VIVO EMBRIO MENCIT (MUS MUCULUS) GALUR SWISS WEBSTER SETELAH PEMBEKUAN DENGAN METODE VITRIFIKASI Madihah, Madihah; Kusumaningtyas, Hartanti; Boediono, Arief; Sumarsono, Sony H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

Reproductive technologies including in vitro fertilization (IVF), embryo manipulation, gamete and embryo freezing, thawing and embryo transfer were rapidly developed. Vitrification is an embryo freezing technique that is the most developed. In this experiment, we vitrified mouse embryos and then examined the embryos i.e: (i) the quality of the embryos after thawing, (ii) the implantation rate of the embryos and (iii) viability of the embryos in vivo. Morulae and blastocycsts were collected from female mice that were pregnant a day 3,5. The embryos were equilibraten in mPBS +10% etilene glycol. Vitrification was carried out by using VABEDS medium, containing 6-10 embryos that were dropped into a tip of a straw, then frozen in liquid nitrogen for 24 hours. Thawing was carried out by flushing the embryos using mPBS suplemented with 0.5, 0.25, 0.1 and 0 M sucrose. After being incubated in M2 medium at 37oC for 1-2 hours, the recovery embryos were then transferred into the uteri of day 2.5 of pseudopregnat females. The females were then sacrificed at day 16 of gestation and the total implantaion, total life and death fetuses, as well as resorpted embryos, were taken as data. The results showed that vitrification significantly (p<0,05) reduced the quality of the embryos, as well as their implantation rate and the viability of the fetuses, which may be caused by the unoptimal combination of the cryoprotectant in the vitrification medium, temperature and exposure time during vitrification.

TINJAUAN PEMANFAATAN RANGGAH RUSA DAN KARAPAS KURA-KURA AIR TAWAR DI PROPINSI PAPUA Semiadi, Gono; Sidik, Irvan
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 1 (2010): February 2010
Publisher : Universitas Atma Jaya Yogyakarta

In Papua, rusa deer (Rusa timorensis) and freshwater turtles constitute the local people bush meat resources through hunting. These hunting activities are produced some by-products i.e. hard antlers from deer and carapace shells from freshwater turtles, where both have a high economic value. The purpose of this study was to investigate the level of production of those by-products and the pathway of the local market being established. The study was conducted at Merauke Regency, Papua, and its vicinity by visiting the key players of all collectors and trade levels. The results showed that on almost all harvested bush meat came from freshwater turtles were cooked on the location, leaving only the carapace shells. Only small quantity of the bush meat was taken home or for trading purposes. However for deer, the majority of hunting activities was done on the purpose for the meat to be sold in the market and small quantity was used for personal needs. Trading on the by-products was conducted at three levels, they were local collectors, middle collectors and local exporter. In general, over 62% of hard antlers samples being observed were uncast hard antlers and 88% of the hard antlers came from the third growth onwards. In a year, at least 1.600-3.700 pairs of hard antlers were acquired, or equivalent to 2.8-6.6 tons. The freshwater turtles being identified were Macrochelodina parkeri, Chelodina reimanni, Macrochelodina rugosa, Elseya braderhorsti and Emydura subglobosa, in which none of the species is under Appendix CITES's list or as Indonesian protected species.

Pertumbuhan Curah Enterococcus faecalis Id 6017 dan Kemampuan Dekolorisasi Reactive Red-2 pada Medium yang Mengandung Gliserol Meitiniarti, V. I.; Napitupulu, Morina M.; Timotius, K. H.
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 1 (2004): February 2004
Publisher : Universitas Atma Jaya Yogyakarta

Enterococcus faecalis ID 6017 can utilize glycerol as the source of carbon and energy for its growth. The present of glycerol in the medium containing Reactive Red-2 not only influenced its growth but also its ability to decolorize Reactive Red-2. The aim of this study was to investigate the growth ofÂ Enterococcus faecalis (E. faecalis) and its ability to decolorize Reactive Red-2. The microbe was grown in batch system with three different growth medium, i.e. medium which contained (i) 1.643 g/l glycerol and 0.08 g/l reactive red-2, (ii) 1.643 g/l glycerol, and (iii) 0.08 g/l Reactive Red 2. The result of this study showed that the growth ofÂ E. faecalis and its ability to decolorize Reactive Red-2 on medium contained glycerol was better than without glycerol. E. faecalis could not growth and decolorized Reactive Red-2 on medium without glycerol.

Peluang Penggunaan Spermatozoa Epididimis Yang Dikoleksi Setelah Kematian Sebagai Sumber Sel Gamet pada Anjing Puja, I Ketut; Trilaksana, IGN; Lontoh, Rudy
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 10, No 2 (2005): June 2005
Publisher : Universitas Atma Jaya Yogyakarta

The purpose of this study was to determine the effect of postmortem time on percentage of life epididymal sperm from postmortem canine caudae epididymides. A total of 9 dog were used and divided into three group. T0 was control group, T1, 3 hours postmortem and T2, 6 hours postmortem. This way, samples were obtained at different times postmortem. Sperm were extracted from the caudae epididymis by means of cuts. The result showed that the percentage of life sperm were 67,16 Â± 5.67(T0), 46.33Â± 5.60 (T1) and 24.00 Â± 4.35 respectively. We could appreciate that percentage of life was affected by postmortem time. There was significant decrease life sperm recovered from epididymides postmortem (P<0.01). In conclusion, epididymal sperm from dog undergo decrease of percentage of life, but it could stay acceptable within many hours postmortem. We intepreted these data to indicate that it may still be possible to obtain viable spermatozoa many hours later.

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Brigitta Laksmi Paramita

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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

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Kota yogyakarta,

Daerah istimewa yogyakarta

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Abstract

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Abstract

Abstract

Abstract

Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA