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Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati

biota
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Published by Universitas Atma Jaya Yogyakarta

ISSN : 25273221 EISSN : 2527323X DOI : doi.org/10.24002/biota

Core Subject : Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology

Biota: Jurnal Ilmiah Ilmu-Ilmu Hayati merupakan jurnal ilmiah yang memuat hasil-hasil penelitian, kajian-kajian pustaka dan berita-berita terbaru tentang ilmu dan teknologi kehayatian (biologi, bioteknologi dan bidang ilmu yang terkait). Biota terbit pertama kali bulan Juli 1995 dengan ISSN 0853-8670. Biota terbit tiga nomor dalam satu tahun (Februari, Juni, dan Oktober).

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 1,193 Documents

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Construction of Soil Metagenomic Library to Obtain Recombinant Clones with an Indigenous Lipase Activity Susanto, Agus Hery; Pramono, Hendro; Lestari, Puji
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 3 (2009): October 2009
Publisher : Universitas Atma Jaya Yogyakarta

Penelitian ini bertujuan untuk mengonstruksi perpustakaan metagenom tanah dari sekitar lokasi industri pengolahan minyak kelapa di Kabupaten Banyumas, Jawa Tengah, dalam upaya mendapatkan klon rekombinan dengan aktivitas lipase indigenuos. Penelitian dilakukan di Laboratorium Genetika, Fakultas Biologi, Universitas Jenderal Soedirman mulai bulan Mei hingga Desember 2006. Secara umum tahapan penelitian terdiri atas ekstraksi dan pemurnian DNA tanah, isolasi pUC19, pemotongan DNA tanah, pemotongan dan defosforilasi pUC19, ligasi fragmen-fragmen DNA tanah ke dalam pUC19 untuk menghasilkan molekul DNA rekombinan, dan transformasi sel inang E. coli JM109 menggunakan molekul DNA rekombinan yang diperoleh untuk mengonstruksi perpustakaan metagenom tanah. Pemotongan DNA tanah menghasilkan fragmen-fragmen yang sebagian besar berukuran lebih kurang 5 kb sehingga gen lipase dimungkinkan untuk dibawa di dalamnya. Transformasi sel inang E. coli JM109 memperlihatkan efisiensi 1.7 x 105 cfu/Î¼g plasmid. Di antara koloni-koloni yang diperoleh, terdapat 102 koloni berwarna putih, yang menunjukkan keberadaan molekul DNA rekombinan. Koloni putih ini dapat digunakan sebagai materi untuk skrining klon rekombinan dengan aktivitas lipase.

The Growth of Giant Clams Juvenil is Influenced by Nutrient Addition Ambariyanto, Ambariyanto
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 2 (2007): June 2007
Publisher : Universitas Atma Jaya Yogyakarta

Ekosistem karang merupakan daerah yang memiliki produktivitas tinggi namun kandungan nutrient anroganiknya sangat rendah. Sehingga dapat diduga bahwa kenaikan nutrient anorganik dalam ekosistem ini akan mempengaruhi kehidupan organisme yang ada di dalamnya. Penelitian ini bertujuan untuk melihat pengaruh peningkatan nutrien yakni N dan P terhadap pertumbuhan juvenil Kima. Hasil penelitian menunjukkan bahwa dengan penambahan nutrien N secara nyata meningkatkan pertumbuhan juvenil Kima tersebut. Di lain pihak penambahan P tidak berpengaruh terhadap laju pertumbuhan juvenil Kima. Hasil ini menunjukkan bahwa penambahan ammonium seharusnya dilakukan di hatchery untuk meningkatkan pertumbuhan dan produksi Kima.

Perilaku Spasio Temporal Tikus Sawah (Rattus argentiventer) Betina Hadi, Susilo; Subagja, Jusup; Sudarmaji, Sudarmaji
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 11, No 2 (2006): June 2006
Publisher : Universitas Atma Jaya Yogyakarta

This research aimed to study spatio temporal behavior of the female ricefield rat, Rattus argentiventer, including home range, range spans, habitat use and daily activity. Twelve rats were released in two locations of a ricefield area from June 11th until July 24th, 2001. The movement of each rat was monitored using radio-tracking three times a day. The result was used for calculating home range size, range spans, habitat used and daily activities of female field-rats, using the computer program Ranges V. The results showed that the average female ricefield-ratâ€™s home ranges and range spans were 0.84 ï‚± 0.16 Ha and 184.54 ï‚± 22.7m respectively. During daylight, the rats spent 82%, 16% and 2% of their activity living in dikes, in villages and in ricefield respectively. On the contrary, during the night 95%, 3% and 2% of the rats were active in the ricefield, villages and dikes respectively.

Diatom dan Paleolimnologi: Studi Komparasi Perjalanan Sejarah Danau Lac Saint-Augustine Quebeq-City, Canada dan Danau Rawa Pening Indonesia Soeprobowati, Tri Retnaningsih; Hadisusanto, Suwarno
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 1 (2009): February 2009
Publisher : Universitas Atma Jaya Yogyakarta

Diatoms are a micro-alga dominates in the aquatic ecosystem. Their silicious cell wall able to preserve death diatoms in the sediment for long periods of time, therefore, diatoms have an important role in the paleolimnological analysis. Diatoms assemblages in the sediment layer express the water quality whenever the diatom lives. This article provides information how to apply diatom on the paleo-limnological analysis, supporting with the case study in the Lac Saint-Augustine Quebec-City Canada and Rawa Pening Lake Indonesia. Modern diatom and the water quality from spatial and temporal range are used as a calibration set. The diatoms of below layers, then, Weighted Averaging (WA) with the calibration set to reconstruct the water quality in the past. Previously, both in Canada and Indonesia, those lakes were oligotrophic and sharply change into eutrophic condition since a lot of human activities developed around the lakes (anthropogenic factors). Naturally, the maturity of lake can not avoid and the succession had been fast by eutrophication. Paleolimnological approach provides baseline data in the past to develop the appropriate lake management.

Purifikasi Kolagenase dari Usus Bandeng (Channos channos, Forskal) Yuniarti, Tatty; Nurhayati, Tati; Jacoeb, Agoes Mardiono
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Collagenase was purified from intestines of milkfish (Chanos chanos, Forskal) by extraction,ammonium precipitation, ion exchange chromatography on DEAE Sephadex A-50 and gelfiltration on a Sephadex G-100 column. The purification and yields were 114.731 fold and1.26% when compared to those in the starting-crude extract. The molecular mass of milkfishserine collagenase was estimated to be 14.63 kDa and 27.46 kDa.

Uji Antagonis Trichoderma sp dan Gliocladium sp terhadap Fusarium Penyebab Penyakit Layu pada Beberapa Jenis Tanaman Pisang di Kebun Raya Purwodadi secara in-vitro Suharjono, Suharjono; Kurniati, Tri Handayani; Soejono, Soejono; Dewi, Susanti
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 9, No 2 (2004): June 2004
Publisher : Universitas Atma Jaya Yogyakarta

Purwodadi Botanical Garden has more than one hundred collection of cultivated bananas. The major problem in keeping their existency isÂ the occuring ofÂ Fusarium wilt diseaseÂ which was commonly controlled by pesticide or eradication to terminate the infectious cycles. Trichoderma and Gliocladium were frequently used as antagonist fungus in controlling the Fusarium wilt disease in kenaf, tomato orÂ the other plants. So,Â this study aims was to know theÂ potency of Trichoderma and Gliocladium on Fusarium growth inhibition. The experimental design was done by Randomized Complete Design Factorial using three factors i.e. antagonist fungus, Fusarium and growth distance. The level of retardation was measured by the growth distance of Fusarium Â that interacted to antagonist fungus and data analysis were conductedÂ by Analysis of Variance (ANOVA). The results showed that Trichoderma has higher inhibition than Gliocladium and the highest inhibition occurred at 1 cmÂ distance of inoculum which was performed on Fusarium 4 (77.78%). There was not any significant differencies between Fusarium 1 with Trichoderma (73.55%) and Fusarium 2 with Gliocladium (73.33%). At 2 cm distance, the highest inhibition occurred in Fusarium 3 by Trichoderma (72.71%), whichÂ was not significantly different with Fusarium 1, 2 and 4. While at 3 cm distance, the highest inhibition on Fusarium 4 by TrichodermaÂ was 51.11% and not significantly different from Fusarium 1, 2 and 3.

Induksi Mutasi Curcuma zedoaria (Christm) Roscoe dengan Irradiasi Sinar Gamma Poerba, Yuyu Suryasari; Martanti, Diah
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 14, No 2 (2009): June 2009
Publisher : Universitas Atma Jaya Yogyakarta

Curcuma zedoaria (Zingiberaceae) is one of Curcuma spp. used as stomachic,carminative, haematic and styptic medicines. Dried rhizomes of C. zedoaria containessential oil and curcuminoids. Induced mutation of this species would provide andhelp plant breeders to improve and select better quality of the plant. The presentstudy was carried out to determine LD-50% of gamma irradiation of C. zedoaria, toinduce genetic variation using gamma irradiation, and to assess genetic diversityamong mutant genotypes using random amplified polymorphic DNA (RAPD)markers. The LD-50% of gamma irradiation was achieved at 813.32 rad. Chlorophyllmutations were recorded at vM1 and vM2. Result of genetic analysis showed thatRAPD profiles were different from that of the control, and genetic dissimilaritiesamong mutant genotypes were higher than that of the control.

Aktivitas Antibakteri Bacillus yang Berasosiasi dengan Landak Laut di Pantai Mentigi, Lombok Barat Suryadi, Bambang Fajar; Febrian, Novi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

The search for new antimicrobial agents is very significant. The prevalence of antimicrobialresistance among key microbial pathogens is increasing at an alarming rate worldwide. Bacillusspecies produce many kinds of antibiotics which share a full range of antimicrobial activities.The aim of this research is to study antibacterial activity of three bacillus isolates (1A, 2J, 3L)which were isolated in Mentigi Beach, West Lombok. Assessment for antibacterial activity wasconducted using Overlaid Molten agar method on Nutrient Agar medium at different salinity(concentration of NaCl 0, 5, 10%) and different pH (pH 6, 7 and 8). The study showed that therewere different antibacterial activities at different salinity and different pH media. Production ofantibacterial at 0% NaCl concentration occurred only at pH 6 and 7 and had narrow spectrumcharacter (only for Gram positive bacteria). Addition of 5% NaCl made antibacterialproduction might occur at pH 6, 7 and 8 and had wide spectrum character (for Gram positiveand negative bacteria).

Seleksi Dan Identifikasi Lactobacillus Kandidat Probiotik Penurun Kolesterol Berdasarkan Analisis Sekuen 16s Rna Triana, Evi; Nurhidayat, Novik
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 12, No 1 (2007): February 2007
Publisher : Universitas Atma Jaya Yogyakarta

High fat and low fiber dietary pattern results in raising of blood cholesterol level over the normal level, namely hypercholesterolemia. Hypercholesterolemia might cause coronary disease and stroke. Blood cholesterol is able to be decreased by probiotic supplement. Lactobacillus is one of the probiotics that were well known and taken advantages. However its role as cholesterol lowering agent was less known. Therefore, screening and identification of Lactobacillus isolates which were candidates of probiotic have been carried out. Isolates Mar 8, Lac 3 and 7 p have been selected as Lactobacillus candidates for cholesterol lowering probiotic. Those isolates met criteria for cholesterol lowering probiotics. Furthermore, they have been conducted to confirm their identity as Lactobacillus. 16S RNA sequences analysis by BLAST analysis against reference strains within DNA Data Bank of Japan (DDBJ) have been carried on. Results showed that sequences of Lactobacillus Mar 8 was 100% homology with Lactobacillus plantarum, Lac 3 was 100% homology with Lactobacillus paracasei and 7 p was 99% homology with Lactobacillus plantarum. It was concluded that the three isolates were selected as candidates for cholesterol lowering probiotics. Both of them, Mar 8 and 7 p, are Lactobacillus plantarum. Another one, Lac 3 is Lactobacillus paracasei.

Variasi Genetik pada Rusa Sambar (Rusa unicolor) di Penangkaran, Kabupaten Penajam, Kalimantan Timur Wirdateti, Wirdateti; Brahmantyo, Bram; Semiadi, Gono; Reksodihardjo, Andi
Biota : Jurnal Ilmiah Ilmu-Ilmu Hayati Vol 15, No 3 (2010): October 2010
Publisher : Universitas Atma Jaya Yogyakarta

Sambar deer (Rusa unicolor) is the largest Indonesian deer species. The largest population of captivation is located at Penajam district, East Borneo (UPTD). First population was introduced in 1990 with four individuals. The use of molecular marker was aimed to identify and characterise the level of genetic diversity within the UPTD population as well as to identify a possible of botolneck population genetic status. This study discussed the relevance of the result for management purposes of captivation. The results indicated that sambar deer populatin (n=38) had an average genetic distance (d) in population as 0.006 with nucleotide diversity (Ï€) being 0.0159). A total of 43.48% of the population was homogeneous that showed no nucleotide differences among individuals.

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Brigitta Laksmi Paramita

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Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

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Abstract

Home Page

OAI Link

Editorial Team

Contact

Reviewer

Google Scholar

Contact Name Brigitta Laksmi Paramita

Contact Email brigitta.laksmi@uajy.ac.id

Phone +6282329549978

Journal Mail Official journal.biota@gmail.com

Editorial Address Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location Kota yogyakarta, Daerah istimewa yogyakarta INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Brigitta Laksmi Paramita

Contact Email
brigitta.laksmi@uajy.ac.id

Phone
+6282329549978

Journal Mail Official
journal.biota@gmail.com

Editorial Address
Fakultas Teknobiologi, Universitas Atma Jaya Yogyakarta, Jalan Babarsari No. 44, Sleman, Yogyakarta 55281, Indonesia

Location
Kota yogyakarta,

Daerah istimewa yogyakarta

INDONESIA