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Isna Rasdianah Aziz
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Department of Biology, Faculty of Science and Technology, Universitas Islam Negeri Alauddin Makassar. Jl. H.M. Yasin Limpo No. 36 Samata, Gowa, South Sulawesi, Indonesia. 92113
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Biogenesis: Jurnal Ilmiah Biologi
Published by Universitas Islam Negeri Alauddin Makassar
ISSN : 23021616     EISSN : 25802909     DOI : https://doi.org/10.24252/bio
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biogenesis: Jurnal Ilmiah Biologi is a peer-reviewed and open-access journal that publishes original scientific work with the advancement of tropical bioscience in Asia. The integration between Islam and tropical bioscience explicitly represents the Biogenesis: Jurnal Ilmiah Biologi as an academic publication for Islamic scientists, which could contribute to the advancement of tropical biology studies in the Islamic world. The journal invites research articles, short communication, critical and comprehensive scientific reviews concerning tropical biosciences in Asia, specifically in the following subjects: Islamic-integrated tropical bioscience Tropical biodiversity and conservation Biosystematics of tropical species Biochemistry and physiology of tropical species Ecological study and behavior of tropical species Tropical genetics, biotechnology, and bioinformatics
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 8 Documents
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Search results for , issue "Vol 11 No 1 (2023)" : 8 Documents clear
Modification of media compositions for micropropagation of Acorus calamus L. Aida Wulansari; Tri Muji Ermayanti; Erwin Al Hafiizh; Betalini Widhi Hapsari; Evan Maulana
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.32129

Abstract

Modification of medium composition is a critical step in tissue culture to find out the best medium formulation for micropropagation. Furthermore, reduction of some nutrients as well as sugar and plant growth regulator concentrations are an effort often done to make the production of seedlings more economic. The aim of the study was to modify media composition by reduction of macronutrients and sugar concentrations in combination with BAP for micropropagation of Acorus calamus. Experiments of Calamus micropropagation was established by culturing single shoots to MS medium with 100, 50, 33.3 and 25% macronutrients added with sugar at 30, 20, and 10 g/l in combination with 0, 0.5, and 1 mg/l BAP. Each medium treatment had nine replicates. Growth of culture was determined every week until 6 weeks of culture. Growth variable recorded was shoots numbers, leaf length, leaf numbers and root numbers. At six weeks of culture, chlorophyll content was also observed. The results showed that reduction of macronutrients and sugar did not influence shoot and leaf numbers in MS medium but required 0.5-1 mg/l BAP. Rooting is best in medium with no addition of BAP. Chlorophyll content decreased in the medium containing BAP in all reduced macronutrients and sugar concentrations. All plantlets survived in the field. Reduction of macronutrients and sugar can be applied for Calamus shoot culture. Cytokinin BAP is critical for shoot growth but this could be omitted for rooting. Therefore, this finding offered more choices in micropropagation of Calamus with low-cost production.
Monitoring of soil transmitted helminth (STH) Helminthiasis on school-age children in final garbage dump, Bantar Gebang, East Bekasi Reza Anindita; Maulin Inggraini
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.32991

Abstract

The prevalence of helminthiasis in West Java is 13.9%, but there is no data on STH helminthiasis on school-age children in the final garbage dump (FGD), Bantar Gebang, East Bekasi, Indonesia. Therefore, it is necessary to monitor STH helminthiasis on school-age children living around FGD. This study aims to determine the description of helminthiasis on school-age children who live in the FGD Bantar Gebang, Indonesia. This type of research is descriptive quantitative with a cross-sectional design. The sample in this study consisted of 79 stool children aged 5-9 years, 5 g of fly vectors from wet, dry, and soil were taken from 36 locations around the FGD. STH examination on stool, flies, and soil samples was carried out using the native, Harada Mori, flotation, and sedimentation methods. All data were analyzed using a descriptive approach to determine the percentage of the incidence of STH. The results of this study, among others, from 79 children there were six (7.60%) children infected with STH with the genus or species Ascaris lumbricoides, hookworm, and Trichuris trichiura. As for the 18 points of soil extraction, there were 12 (66.7%) positive points containing hookworm larvae, while the flies found were more dominant in Musca domestica than Chrysomya sp. with worm parasites attached to the fly's body are Ascaris lumbricoides, Diphyllobothrium latum. Enterobius vermicularis, hookworm, Hymenolepis sp., Schistosoma sp., Trichuris trichiura. The conclusion of this study is percentage incidence of STH helminthiasis on school-age children living in FGD Bantar Gebang is 7.60% which is in the low category.
Candidates for antimalarial compounds in secondary metabolites of Streptomyces sp. InaCC 1497 and AB8 Endah Setyaningrum; Jihan Fikra Angelia; Kusuma Handayani; Achmad Arifiyanto; Dzul Fithria Mumtazah
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.33727

Abstract

One effort to reduce the transmission of malaria is the provision of antimalarial drugs. However, the use of drugs that are not according to standards causes resistance to Plasmodium. This condition triggers the exploration of various natural compounds to prevent malaria. Secondary metabolites derived from Streptomyces sp. are known to have antimalarial activity. However, information related to secondary metabolites from Streptomyces sp. strains InaCC A497 and AB8 as antimalarials are not yet known. The purpose of this study was to determine the metabolite compounds secondary contained in Streptomyces sp. strains InaCC A497 and AB8 as antimalarial candidates. This research method is descriptive by testing secondary metabolites which are carried out through chemical compound tests, thin layer chromatography (TLC), fourier transform infra-red (FT-IR), and gas chromatography-mass spectroscopy (GC-MS). Test results of chemical compounds and FT-IR analysis of Streptomyces sp. InaCC A497 contains alkaloids, flavonoids, and triterpenoids, while Streptomyces sp. AB8 contains alkaloids and tannins. In the TLC test, Streptomyces sp. InaCC A497 produced an Rf value of 0.257 and Streptomyces sp. AB8 of 0.314. Based on the GC-MS test, Streptomyces sp. InaCC A497 produces acetic acid compounds, ethyl ester including the ester group, and 1,2-benzene dicarboxylic acid, dioctyl ester including the alkaloid group. Streptomyces sp. AB8 produces the compound 2-pentadecyn-1-ol which belongs to the aromatic alcohol group and cochlioquinone A belongs to the quinone group. Both compounds have antimalarial activity.
The effect of commercial herbicide and bioherbicide of Ketapang (Terminalia catappa L.) on germination and chlorophyll content of cayenne pepper (Capsicum frutescens L.) Rita Ariyana Nur Khasanah; Silvia Rahmawati
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.33881

Abstract

Herbicides that have less impact on non-target crops are better suited for weed control. The use of a commercial glyphosate-based herbicide (Roundup®) is a farmer's choice for controlling these weeds. The negative effects of glyphosate residues on cultivated crops, environment and human health have led farmers to switch to use bioherbicides. Ketapang (Terminalia catappa L.) leaves have been studied having the potential as bioherbicide but their effects on non-target plant have been less studied. This study aimed to examine the effects of Roundup® and Ketapang leaf extract on germination and chlorophyll content of cayenne pepper as non-target plant. This experimental study was conducted using completely randomized design (CRD) with 3 replications. Cayenne pepper was sown for 14 days. Parameters observed were germination, morphology, weight, and chlorophyll content of cayenne pepper sprouts. The data were analyzed using SPSS ver. 16 and tested with One-Way ANOVA or Kruskal Wallis tests at p-value of 0.05 depended on parametric assumption tests. There were significant differences between the Roundup® solution treatment with the control on all parameters (germination parameters, weight, and chlorophyll content of cayenne pepper sprouts) (p<0.05). The effect was getting worse seen in cayenne pepper seeds given high doses of Roundup® solution. On the contrary, there were no significant differences between the treatments of the aqueous extract of Ketapang leaves with the control on all parameters (p>0.05), except the vigor index. There were morphological changes in the radicle and root of cayenne pepper sprouts exposed to them. Rotted radicles were highly visible in Roundup®-exposed sprouts.
The Variation and phenetic relationships of Dendrophthoe pentandra (L.) Miq. from various host trees based on morphological characters Novario Permata Adiansyah; Putri Dian Islami; Purnomo Purnomo
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.34540

Abstract

Dendrophthoe pentandra (L.) Miq.; Loranthaceae) is a shrub parasitic on various host tree species. This species is often used for herbal medicinal ingredients by local communities based on the spesies of host tree. The morphological characteristics of D. pentandra are important to study because their adaptation to different host trees may resulting population of ecotypes. This study aims to determine the variation of D. pentandra accession characters on different host tree species based on morphological characters and to determine morphological phenetic relationships between accessions. Besides studies of D. pentandra accessions in Yogyakarta Province have never been conducted in full. Sample collection (accession) of D. pentandra was conducted for various host tree species from various locations, including roots, stems, leaves, flowers, and fruit. Morphological characterization and scoring of each accession were conducted to create a similarity matrix using the Jaccard Coefficient formula. Based on the similarity matrix between OTUs and the UPGMA algorithm, it can be formed into a dendrogram. The results showed that the morphological variations of D. pentandra were found in phyllotaxis, leaf thickness, petiole length, number of stomata density per field of view, flower color, crown tube length, corolla lobe length, petal length, stamen : corolla lobe ratio, fruit color, and seed color. The population of D. pentandra in Yogyakarta can be divided into two groups, D. pentandra which has alternate and opposite arrangements in phyllotaxis. The accession dendrogram of D. pentandra in Yogyakarta was divided into five clusters starting from a similarity index of 0.089 to 0.952. Thus, the diversity of morphological variations of D. pentandra on host trees is high.
Study on the Genus Plectranthus (Lamiaceae) in Java: P. verticillatus, a captivating new alien species Arifin Surya Dwipa Irsyam; Muhammad Rifqi Hariri; Muhammad Fabio Rayhan Kurniawan; Rina Ratnasih Irwanto
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.35297

Abstract

Plectranthus (Lamiaceae) is a popular ornamental plant all over the world. The study of the genus Plectranthus in Java was considered complete after the publication of Flora of Java Vol. II. Many authors, however, have reported the presence of numerous alien species on the island in recent years. The aim of this research is to provide current information on Plectranthus in Java, particularly the newly recorded alien species. The study was carried out using the free exploration method in the provinces of Banten, Jakarta, West Java, and East Java. We reported the first occurrence of P. verticillatus (L.f.) Druce in Java in this paper. The species is a succulent herb native to Southern Africa that has grown in popularity as an ornamental plant throughout the world. It is a newly discovered alien species to the Alien Flora of Java. The species appears to have escaped cultivation, with spontaneous populations found in Jatihandap and Cipadung, both in Bandung City. We also reported that P. verticillatus is the only member of Plectranthus found in Java in a recent taxonomic study. There is a description, photographs, botanical illustrations, and a brief discussion.
Isolation and potency of Actinomycetes from rhizosphere of nutmeg (Myristica fragrans Houtt) Ferymon Mahulette; Esti Utarti; Tri Santi Kurnia
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.35632

Abstract

Nutmeg (Myristica fragrans Houtt) is commonly cultivated by people in the forests of Moluccas Islands. This plant grows well on relatively infertile soil types. This is presumably due to the presence of symbiotic microbes in the root of nutmeg. The research aimed to isolate, characterize and test the potential of Actinomycetes from rhizosphere of nutmeg. Soil sample were taken from the nutmeg forest in Ambon Island. The Actinomycetes isolation using humic acid vitamin, continued with yeast malt agar (YMA) media. The testing of antibacterial and antifungal activities using YMA media, while cellulolytic activity, phosphate solubilizing, and xylanolytic activity using carboxyl methyl cellulose, Picovskaya agar, and birchwood agar or oat spelt xylan agar. A total of 12 isolates of Actinomycetes were isolated and dominated by Streptomyces with various types of aerial mycelia. The substrate mycelium looks brown and cream, while the aerial mycelium looks white and gray. These isolates had the highest inhibitory power against Escherichia coli and Fusarium oxysporum with indexes of 16.5 mm and 16.0 mm, respectively. The other isolates have the ability of cellulolytic, phosphate solubilizing, and xylanolytic with indexes 3.26, 3.87, and 1.2, respectively. The Actinomycetes isolates that were found can be used as starter to improve the biofertilizer formula for nutmeg.
Neuronal cell death in the amygdala and cerebral cortex of mice (Mus musculus) induced by bee (Apis mellifera) venom Rian Oktiansyah; Ummi Hiras Habisukan; Anggun Wicaksono; Yustina Hapida; Fahmy Armanda
Biogenesis: Jurnal Ilmiah Biologi Vol 11 No 1 (2023)
Publisher : Department of Biology, Faculty of Sci and Tech, Universitas Islam Negeri Alauddin Makassar

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.24252/bio.v11i1.36165

Abstract

Brain is an organ to control our activities, such as human consciousness, emotional, and movement. It was controlled by amygdala and cerebral cortex as the parts of the brain. Many things that can bring over it. Bee venom (BV) is known as traditional medicine and probably can sway the brain. The objective of this study was to determine the dose of BV that causes excessive neuronal cells death, especially in the amygdala and cerebral cortex. About 15 white male mice Deutsch Denken Yoken (DDY) strain were divided into control group and the treatment group. BV was administrated intraperitoneally for two weeks with multilevel doses, that was 1.88 mg/kg, 3.76 mg/kg, 5.6 mg/kg, and 7.48 mg/kg. Brain tissue isolation was performed three days of the last administration by using perfusion method. Morphological sectioned of brain tissue (amygdala and brain cortex) was stained by hematoxylin-eosin (HE). The results indicated that the BV inclined to affect neuronal cells death in the amygdala and cerebral cortex. Based on the study, the highest doses (7.48 mg/kg) of BV caused the highest neuronal cell death.

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