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INDONESIA
Indonesian Journal of Agricultural Science
Published by Kementerian Pertanian
ISSN : 1411982X     EISSN : 23548509     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
The journal publishes primary research articles from any source if they make a significant original contribution to the experimental or theoretical understanding of some aspect of agricultural science in Indonesia. The definition of agricultural science is kept as wide as possible to allow the broadest coverage in the journal. Accredited base on Director Decree of Indonesian Institute of Science No. 816/D/2009 with acreditation No. 172/AU1/P2MBI/08/2009. IJAS is newly accredited by the Indonesian Institute of Science in 2012 (No. 473/AU2/P2MI-LIPI/08/2012) for period of 2012-2015.
Arjuna Subject : -
Articles 352 Documents
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SOIL PROPERTIES OF THE EASTERN TOPOSEQUENCE OF MOUNT KELIMUTU, FLORES ISLAND, EAST NUSA TENGGARA AND THEIR POTENTIAL FOR AGRICULTURAL USE ., Hikmatullah; Subagyo, H.; Prasetyo, B.H.
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Kelimutu stratovolcano is one of the young volcanoes extensively found in Flores island. Studies on volcanic soil properties and their suitability for agricultural development in the whole island are lacking. The research was conducted to evaluate the relationship of elevation and soil properties,  and suitability for agricultural development. Five representative pedons at elevation of 550, 1,000, 1,200, 1,400, and 1,600 m above sea level (asl), respectively, were studied in the field, and 22 soil samples were analyzed in the laboratory. The results indicated that elevation significantly affected soil properties and degree of soil weathering. With decreasing elevation, sand content, amorphous material content, and phosphate retention decreased. Concomitantly, clay content, H2O-pH, exchangeable Ca and Mg, base saturation, and soil-CEC increased. A highly positive correlation was shown between P retention and NaFpH, Al, and (Al+0.5 Fe) contents extracted by acid ammonium oxalate. Soil-CEC also showed significant positive correlation with clay and silt contents, organic carbon, and exchangeable Ca and Mg. Degree of weathering increased with decreasingelevation as reflected by decreasing silt/clay ratio. Soils developed from Entisols (Lithic Udorthents) at 1,600 m asl, to Andisols (Typic Hapludands) at 1,000-1,400 m asl, and Mollisols (Typic Hapludolls) at lower elevation down to 550 m asl. Soils at 1,400-1,600 m asl are unsuitable for  agriculture. Soils at 1,000-1,200 m are moderately suitable for food crops,horticulture, and estate crops. Dominant limiting factors are steep slopes and P retention. Soils at 550 m asl are suitable for food and estate crops, and also lowland horticulture.
DETERMINATION OF FOLATE RETENTION DURING TOFU PREPARATION USING TRIENZYME TREATMENT AND MICROBIOLOGICAL ASSAY Ginting, Erliana; Arcot, Jayashree; Chox, Julian M.
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Megaloblastic anaemia, neural tube defects, coronary heart disease, and some forms of cancer have been linked to an inadequate dietary intake of folate. Legumes are good sources of folate, however, processing may affect folate availability in legume foods. This study investigated the folate content and retention during the preparation of tofu through the trienzyme treatment (protease, α-amylase, and human plasma) and microbiological assay using Lactobacillus casei. The results showed that the total folate content was considerably reduced during tofu preparation, from 273.4 µg 100 g-1 dwb in soybean seed to 108.4 µg 100 g-1 dwb in tofu, yielding a folate retentionof 40%. Folate losses were mainly due to leaching into the water during soaking and pressing as well as heat destruction during milling and boiling. High folate loss (51%) was particularly found in the whey. A shorter soaking time (8 hours), optimum extraction, and pressing methods were suggested with respect to minimizing folate losses during tofu preparation. This studysuggests that the consumption of 100 g of deep-fried tofu may satisfy 21% of the recommended dietary intake (RDI).
PRODUCTION OF POLYCLONAL ANTIBODY TO THE COAT PROTEIN OF CITRUS TRISTEZA VIRUS IN CHICKEN EGGS ., Nurhadi; Sijam, Kamaruzaman; Sulaiman, Inon
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Citrus tristeza virus (CTV) is one of the most destructive diseases in many citrus growing areas of Indonesia. Effective strategies for controlling CTV depend on diagnostic procedure namely enzyme-linked immunosorbent assay (ELISA). Study aimed to purify the CTV antigen and produced its polyclonal antibody. Virion of the severe CTV isolate designated UPM/T-002 was concentrated by polyethylene glycol (PEG) precipitation combined with low speed centrifugation. Semipurified antigen was further purified by sodium dodecyl sulphatepolyacrylamide gel electrophoresis (SDS-PAGE). The specific coat protein (CP) band of CTV with molecular weight of 25 kD was excised and eluted using elution buffer containing 0.25 M Tris-HCl pH 6.8 + 0.1% SDS, then used as antigen for injectioninto 6-month-old female of White Leghorn chicken. Results, showed than the specific polyclonal antibody raised against the 25-kDa CP had a titer of approximately 104, gave low background reaction with healthy plant sap and reacted specifically with CTV isolates. The reaction was equally strong for a severe, a moderate, a mild, and a symptomless isolate, suggesting a broad reaction range of this antibody toward different CTV isolates. Optimal virus titer can be obtained since virus loss during purification could be minimized and the highly purified antigen as an immunogen could be obtained by cutting out the CP band from SDS-PAGE gels. Large amount of highly titer of CTV antibody can be produced in chicken egg. The simplicityof the procedure makes it economically acceptable and technically adoptable because the antibody can be produced in basic laboratory.
SCREENING OF SOIL BACTERIA FOR PLANT GROWTH PROMOTION ACTIVITIES IN VITRO Husen, Edi
Indonesian Journal of Agricultural Science Vol 4, No 1 (2003): April 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Fourteen isolates of soil bacteria, including two known plant growth promoting rhizobacteria (PGPR) strains, Azotobacter vinelandii Mac 259 and Bacillus cereus UW 85, were tested in vitro. Parameters assessed were indoleacetic acid (IAA) production, phosphate solubilization, dinitrogen fixation, and siderophore (Fe-III chelating agent) production. IAA production was assayed colorimetrically using ferric chlorideperchloricacid reagent. Phosphate-solubilization and siderophore production were tested qualitatively by plating the bacteria in Pikovskaya and chrome azurol S agar, respectively. The ability to fix dinitrogen was measured based on nitrogenase activity of the bacteria by gas chromatography. The results showed that twelve isolates produced IAA, ranged from 2.09 to 33.28 µmol ml-1. The ability to solubilize precipitated phosphate waspositively exhibited by four isolates (BS 58, BTS, TCaR 61, and BTCaRe 65). Seven isolates including Mac 259 positively produced siderophore. None of the isolates showed nitrogenase activity. Only one isolate (TS 3) did not exhibit any of the traits tested. Isolate TCeRe 60 and reference strain Mac 259 were found to have IAA- and siderophore-producing traits. Four P-solubilizing bacteria (BS 58, BTS, TCaR 61, and BTCaRe 65)were also IAA- and siderophore-producing bacteria. Potential use of these PGPR isolates needs further test in enhancing plant growth.
ESTIMATING SOYBEAN PRODUCTION EFFICIENCY IN IRRIGATED AREA OF BRANTAS RIVER BASIN Siregar, Masdjidin; ., Sumaryanto
Indonesian Journal of Agricultural Science Vol 4, No 2 (2003): October 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Soybean has an important role in Indonesian diet, especially as source of protein, fat, mineral, and vitamin. Given the relatively stagnant technology, efficiency improvement at farm level would probably be an appropriate way to increase soybean production in the near future. The objective of this paper was to estimate the technical efficiency of soybean production in irrigated area of Brantas river basin and analyze factors affecting technical inefficiency level. The results indicated that the technical efficiency of soybean production in the sites was around 83%. The analysis, however, failed to identify the determinants of technical inefficiency because none of the parameters in the analysis was significant. Further study is required to identify the determinants such that the target groups of extension can be specifically determined. Since K2O fertilizer significantly affected soybean production while P2O5 and N fertilizers did not, the first step to improve the technical efficiency of soybean production was to provide soybean farmers with recommendation regarding balanced amount of fertilizers. The recommendation, however, should be derived from local verification trial in each site.
IDENTIFICATION AND EVALUATION OF FIBER HYDROLYTIC ENZYMES IN THE EXTRACT OF TERMITES (Glyptotermes montanus) FOR POULTRY FEED APPLICATION Purwadari, Tresnawati; Ketaren, Pius P.; Sinurat, Arnold P.; Sutikno, Irawan
Indonesian Journal of Agricultural Science Vol 4, No 2 (2003): October 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Poultry are not able to digest fiber in the diet. Hydrolytic enzymes including cellulases and hemicellulases have been used as poultry feed supplement. Termites (Glyptotermes montanus) have the ability to digest wood that contains high fiber. The purpose of this experiment was to identify the cellulase and hemicellulase of termite extract. The hydrolytic (saccharification) activity of the termite extract on feedstuffs was thenevaluated. It contained high endo-β-D-1,4-glucanase (CMCase) activity, but the activities of avicelase, β-D-1,4-mannanase, β- D-1,4-xylanase, and β-D-1,4-glucosidase were very low. The activities of the enzymes were higher in the fresh extract than those extracted after drying at 40oC with blower oven. CMCase (as cellulase), β-D-1,4-mannanase (as  hemicellulase), and β-D- 1,4-glucosidase (as glycosidase) were reevaluated further to determine the optimum pH and temperatures for maximum activities. The optimum pH for CMCase, β-D-1,4-mannanase,and β-D-1,4-glucosidase were 6.2, 5.0, and 5.8 respectively, while the optimum temperatures were 45-50oC, 50-55oC, and 42-45oC, respectively. The enzyme mixture or cocktail was more appropriate in digesting feedstuffs with high lignocellulose (fiber) such as rice bran and pollard than feedstuffs with more soluble starch such as soybean and corn meals. The extracted enzyme could be immobilized with pollard, butCMCase recovery was low (28.6%), while β-D-1,4-mannanase and β-D-1,4-glucosidase recoveries were 89.2% and 272.9%, respectively. Termite extract contained enzyme cocktails of lignocellulases that potentially be used as feed supplement. However, its use is limited by its low activity.
PERFORMANCES OF TIGER SHRIMP CULTURE IN ENVIRONMENTALLY FRIENDLY PONDS Ahmad, Taufik; Tjaronge, M.; Suryati, E.
Indonesian Journal of Agricultural Science Vol 4, No 2 (2003): October 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Mangrove ecosystem plays an obvious role in maintaining the biological balance in the coastal environment where shrimp ponds are usually constructed. The removal of mangroves around shrimp ponds has frequently brought about harvest failure. The study evaluated the performance of tiger shrimp culture in ponds provided with water from a water body where there was mangrove vegetation (hereafter mangrove reservoir). Twelve ponds, each measuring 2,500 m2, were filled with seawater from the mangrove reservoir until the water depth of 100 cm and then stocked with 20-40 PL/m2. In the first six ponds, the bottom water was released into the reservoir when the water depth reached 140 cm and then the water depth was maintained at 100 cm. In the second six ponds, the water was released from the ponds until the water depth reached 60 cm and then refilled with reservoir water until a depth of 100 cm. Both treatment ponds received water from the reservoir which also received the wastewater. The feeds for the shrimps were broadcast into the ponds twice a day to meet the 3% shrimp biomass requirement, which adjusted every other week through sampling. The result showed that mangrove  vegetation is capable of removing excessive nutrients, up to 70% for NO3-N and NH4 +-N, reducing PO4 =-P fluctuation, and producing bioactive  compounds. In the second treatment ponds, shrimp mortality started to occur in day 28 and most died by day 54 after stocking due to white spot disease outbreak. Mass mortality took place 54 days after stocking in two out of six of the first treatment ponds.
IMPROVEMENT OF SEEDLING ESTABLISHMENT OF WET SEEDED RICE USING GA3 AND IBA AS SEED TREATMENT Wahyuni, Sri; Sinniah, Uma R.; Yusop, Mohd. Khanif; Amarthalingam, Rajan
Indonesian Journal of Agricultural Science Vol 4, No 2 (2003): October 2003
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Direct seeding has some advantages compared to transplanting system in rice, however there are some constraints need to be addressed. One  constraint generally faced in the wet seeded rice is poor stand  establishment. The experiment was conducted to improve seedling  establishment by using selected growth regulators. Seeds of four rice  cultivars, i.e. Memberamo, Widas, MR 84, and MR 219 were treated with  GA3 (gibberellic acid-3) of 25, 50, and 100 mg 1-1, and IBA (indole-3  butyric acid) of 10, 20, and 40 mg l-1. Results showed that cultivar  response to GA3 was similar in all parameters observed, however the response of cultivars to IBA was different at the initial stage. In Indonesian cultivars (Memberamo and Widas), shoot length of IBA treated seedlings was comparable to the control, but the IBA treated seedlings of  Malaysian cultivars (MR 84 and MR 219) had slightly shorter shoots   compared to the control. GA3 as seed treatment induced favorable early emergence and shoot growth in the initial stage, but the shoots were  etiolated and resulted in susceptible to lodging even in the vegetative  stage. In comparison, exogenous application of IBA resulted in high number of adventitious roots contributing towards better establishment of seedling with broader and greener leaves. The growth regulator was lost its  effect by 2 or 3 weeks after sowing. All IBA concentrations also did not  show any significant differences on growth parameters or have any detrimental effect on grain yield in all cultivar tested. IBA 10 mg l-1 was  sufficient in providing the required improvement in the rice seedlings.
SOIL MYCOFLORA OF BLACK PEPPER RHIZOSPHERE IN THE PHILIPPINES AND THEIR IN VITRO ANTAGONISM AGAINST Phytophthora capsici L. Noveriza, Rita; Quimio, Tricita H.
Indonesian Journal of Agricultural Science Vol 5, No 1 (2004): April 2004
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Abstract

Foot rot disease of black pepper caused by Phytophthora capsici had been reported in Batangas and Laguna, Philippines. The plant was recovered following the application of crop residue (organic substrate) and intercropping with other crops. This study was aimed to isolate, identify, and determine the soil mycoflora from the rhizosphere of black pepper grown on various cropping patterns in Batangas and Laguna. Antagonisticactivity of mycoflora isolates was tested against P. capsici using dual culture technique. The result showed that 149 colonies of soil mycoflora isolated were belonging to 14 genera; three of them, i.e. Penicillium, Paecilomyces and Aspergillus, were the most dominant. All of the mycoflora isolates were able to inhibit the growth of the pathogen. Eighteen of them were the most promising antagonists, based on their inhibition growth of more than 60%. It is suggested that antagonistic mechanism of Mucor isolate (1001), Trichoderma (125, 170, 171, 179, 180, 181), Gliocladium (109), Cunninghamella (165, 168), Mortierella (177), and Aspergillus (106) was space competitor (competition for nutrient) since they rapidly overgrew the pathogen. Aspergillus (67, 79, 81, 83, 108, and 202) isolates inhibited the pathogen apparently by producing antibiotic, whereas Trichoderma (125, 170, 171, 179, 180, and181) isolates were able to penetrate the hyphae of the pathogen. The organic matter percentage in the soil was significantly correlated with the number of antagonistic mycoflora in rhizosphere (R2 = 0.1094), but the cropping pattern was negatively correlated. This study suggests that organic matter increased antagonistic mycoflora in black pepper rhizosphere, which will reduce severity of the disease.
EFFECT OF PERMEABLE VESSEL CLOSURE AND GELLING AGENT ON REDUCTION OF HYPERHYDRICITY IN IN VITRO CULTURE OF CARNATION Winarto, B.; Aziz, M.A.; Rashid, A.A.; Ismail, M.R.
Indonesian Journal of Agricultural Science Vol 5, No 1 (2004): April 2004
Publisher : Indonesian Agency for Agricultural Research and Development - MOA

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Hyperhydricity, an abnormal morphological appearance and physiologicalfunction, is an important problem in carnation tissue culture. The problem causes premature flowering, high occurrence of abnormal shoots, difficulty in transferring hyperhydric plantlets to soil, and low survival rate of plantlets. High relative humidity and the water potential are considered as the key factors involved in the abnormality. Furthermore, permeable culture vessel and gelling agent were assured to be high potential treatment to eliminate it. Objective of this research was to reduce  hyperhydricity in regenerants of carnation using different permeable vessel closures and gelling agents and to assess the multiplication and  acclimatization abilities of recovered shoots. Experiment was arranged in randomized complete block design with four replications. First factor was different types of closure, i.e. cotton wool, plastic wrap, parafilm and aluminium foil, while second one was gelling agents, i.e. bacto agar, phytagel, swallow agar, and Type 900 agar. The recovered shoots were then multiplied, rooted, and acclimatized. The results showed that hyperhydricity was successfully reduced by applying permeable closure (cotton wool and plastic wrap) in combination with Type 900 agar. Thecombination of plastic wrap and Type 900 agar was the most appropriate treatment in reducing hyperhydricity and producing good quality shoots. The treatment reduced the problem down to 23% of total condition of hyperhydricity (100%) and increased leaf chlorophyll content from 0.0883 to 0.1288 mg mg-1. The plastic wrap was easily applied and cheaper material compared to cotton wool. The recovered shoots were able to produce 1-3 healthy axillary shoots and easily rooted on half-strength MS. The recovered plantlets were simply acclimatized with survival rate upto 100% on kossas peat + soil (1:1, v/v) and flowered 4-5 months after acclimatization with decreasing in number and size of flower.

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