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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 7 Documents
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Search results for , issue "Vol 16, No 1 (2020): June" : 7 Documents clear
Genetic Diversity of Butterfly Pea (Clitoria ternatea) from Indonesia Based on Flower and Yield Component Traits in Two Land Conditions Trixie A. Ulimaz; Debby Ustari; Virda Aziza; Tarkus Suganda; Vergel Concibido; Jutti Levita; Agung Karuniawan
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p1-6

Abstract

Genetic diversity among the butterfly pea genotypes is important information to support breeding program of this underutilized crop. The important characters to be targeted in the breeding program of this crop included yield and yield components of flowers that are strongly affected by the environment and have not been previously reported. This study aimed to determine the genetic diversity of butterfly pea (Clitoria ternatea L.) from Indonesia tested in two land conditions, namely dryland and former paddy fields, based on flower character and yield component traits. The results showed that butterfly pea accessions were divided into two main clusters with dissimilarity coefficient of 0.01–3.99 indicating wide genetic diversity across  accessions. The Mantel correlation showed that the genetic distance among accessions studied were not significantly correlated (r = 0.044, P = 0.8709). Based on principal component analysis (PCA), the eigenvalue ranged from 1.69 to 3.34 with a cumulative contribution of 72.64%. The traits that influenced genetic diversity in this study were flower length, weight of one fresh flower, total weight of pods, and weight of 100 seeds. The results of this study should be useful to support future butterfly pea breeding program.
Construction of Binary Vector and Transformation of Synthetic LcCsp Gene into Nipponbare Rice Genome by Agrobacterium tumefaciens Transformation Method Dina Sri Yulita; Aqwin Polosoro; Atmitri Sisharmini; Aniversari Apriana; Febi Nurilmala; Kurniawan Rudi Trijatmiko
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p25-34

Abstract

Cold shock protein (Csp) is an essential bacterial protein for increasing abiotic stress tolerance, especially cold stress. Several studies discovered that overexpression of the gene successfully improves the tolerances of several types of plant not only under cold stress, but also other abiotic stresses, e.g. hot and drought conditions. The objectives of this study were to construct a binary vector containing the LcCsp gene modified from Lactobacillus casei and transform the gene into Nipponbare rice genome. The native LcCsp gene sequence, however, has low GC content (46.7%) while rice as transformation target plant has 52% GC content. The native LcCsp gene sequence, therefore, was optimized to the level close to 52.7% similar to GC content of the rice genome. This LcCsp gene was synthesized by using DNA printing technology (gBlocks® Gene Fragments Entry, IDT). The synthetic LcCsp gene was successfully inserted into the pCAMBIA1300-int binary vector driven by Ubiquitin1 promoter and NOS terminator. The T-DNA cassette was successfully transformed into Nipponbare rice genome by Agrobacterium tumefaciens strain LBA4404 using immature embryo transformation protocol. A total of 51 T0 Nipponbare lines survived from hygromycin selections and 21 lines were successfully acclimatized. Molecular analysis of the candidate lines showed that all Nipponbare transgenic putative lines contain the LcCsp gene demonstrating high transformation efficiency of 11.8%. The rice lines resulted from this study should be further analyzed and might be useful for developing rice transgenic lines tolerance to heat, drought, or saline stress condition.
Validating Plant Genes Involved in Pepper Yellow Leaf Curl Indonesia Virus Infection Using VIGS in Model Plant Nicotiana benthamiana Kusumawaty Kusumanegara; Masanori Kaido; Kazuyuki Mise
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p7-16

Abstract

Pepper yellow leaf curl disease caused by Pepper yellow leaf curl Indonesia virus (PepYLCIV) has become a challenge to chili pepper cultivation. Development of resistant variety by utilizing recessive resistance gene is expected to control the disease in the field. This study aimed to validate three plant genes, namely deltaCOP, hsc70, and BAM1, in PepYLCIV infection by applying Virus-induced Gene Silencing (VIGS) in a model plant, wild type Nicotiana benthamiana. PepYLCIV and construct of Tobacco rattle virus (TRV) which induced silencing of each gene were co-inoculated into N. benthamiana plants through agroinfiltration. Gene expression and the relative amount of viral DNA were determined by quantitative reverse transcription PCR (qRT-PCR) and quantitative PCR (qPCR), respectively, at 15 days post inoculation. The results showed a decreased level of deltaCOP, hsc70, and BAM1 expressions to 66.4%, 53.0%, and 47.0%, respectively, compared to that in the control (100%). Silencing of the three genes decreased the accumulation of PepYLCIV to 0.1%, 18.4%, and 63.0%, respectively, compared to that in the control. deltaCOP and hsc70 genes were indicated to be involved in the viral infection and could be good candidate genes for obtaining chili pepper varieties resistant to PepYLCIV. This result affirmed that the reverse genetics technique could be an alternative approach for identifying plant genes involved in viral infection, including PepYLCIV. The use of an infectious clone in this study allows the virus inoculations could be carried out without rearing and maintaining its natural vector, hence reduces the risk of virus transmission to healthy plants.
Confirmation of Alleles Inheritance in F1 Progenies Derived from a Cross of Calcutta-4 (Musa acuminata ssp. burmannicoides) and Musa acuminata ssp. microcarpa Based on SSR Markers Dea Rosalia; Puji Lestari; Andy Soegianto; Darmawan Saptadi; Agus Sutanto; Kristianto Nugroho; Rerenstradika T. Terryana; I Made Tasma; Ika Roostika
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p17-24

Abstract

Banana breeding to produce improved varieties with disease resistance haracters and other desired traits could sustain its yield. Alleles harbored by parents could be passed on to the offsprings through hybridization, but need to be confirmed using molecular markers. This study aimed to confirm allele inheritance in F1 progenies derived from a cross of Calcutta-4 (Musa acuminata ssp.  burmannicoides) and M. acuminata ssp. microcarpa based on SSR markers. Eleven pairs of SSR primers were used to amplify DNA of 44 progenies using the PCR technique. The results showed that six SSR markers (MaSSR 1.1, MaSSR5.1, MaSSR 6.1, MaSSR 7.1, MaSSR 8.1, and MaSSR 11.1) were polymorphic in both parents. Four markers (MaSSR 1.1, MaSSR 5.1, MaSSR 6.1, and MaSSR 8.1) had PIC >0.7, indicating their informativeness to distinguish these progenies and other genetic studies of banana germplasms. A total of 44 F1 individuals were confirmed to harbor alleles inherited from their parents,suggesting as true progenies from the cross of Calcutta-4 and M. acuminata ssp. microcarpa. This population demonstrated 100% success of hybridization performed. Chi-Square analysis revealed that segregation of all markers did not match to Mendelian ratio 1:2:1, except for MaSSR 1.1 (x2 = 5,62) and MaSSR 6.1 (x2 = 3,77) markers. The genetic traceability of banana F1 progenies demonstrating the usefulness and feasibility of SSR markers in this study provided information on selection of true progenies which may be valuable for breeders to assist selection process in future banana breeding program in Indonesia.
Molecular and Phenotypic Analyses of Inpari HDB/K15 F2 Lines Containing sd1 Mutant Gene Resulted from Genome Editing Method Clara S. A. Fitriyanti; Suharsono Suharsono Suharsono; Tri Joko Santoso
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p35-44

Abstract

Inpari HDB variety is resistant to bacterial leaf blight (BLB) disease, but due to its tall stature, the variety is susceptible to lodging. Inpari HDB with semidwarf stature, therefore, is of high interest for lodging resistant performance. sd1 gene encoding GA20ox-2 enzyme is one of the genes responsible for imparting semidwarf stature of rice. In previous study, sd1 mutant rice cv. Kitaake (K15) was developed by using CRISPR/Cas9 technology. The objective of this study was to analyze molecularly and phenotypically F2 lines containing sd1 mutant gene resulted from a cross between Inpari HDB and K15 to develop semidwarfInpari HDB rice variety. Thirty F2 Inpari HDB/K15 lines were analyzed at molecular level using DNA sequencing method together with phenotypic assessment of the lines to verify the integration of sd1 mutant gene. DNA sequencing analysis showed that 9 out of the 30 F2 Inpari HDB/K15 lines were sd1 mutants. The remaining F2 lines contained 17 heterozygotes and 4 nonmutants. All the nine mutant lines demonstrated shorter plant stature and showed more tiller number per plant compared to the nonmutant lines. The sd1 mutant gene in the F2 lines showed pleiotropic effects on panicle number andshowed no effects on other traits, such as flowering time, panicle length, filled and unfilled grain percentages. This study showed the introduction of sd1 mutant gene generated semidwarf Inpari HDB lines. The semidwarf Inpari HDB lines obtained from this research should be further evaluated to confirm their lodging resistant performances.
Front Matter JA Vol 16 No 1 Agrobiogen, Jurnal
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p%p

Abstract

Back Matter JA Vol 16 No 1 Jurnal Agrobiogen
Jurnal AgroBiogen Vol 16, No 1 (2020): June
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v16n1.2020.p%p

Abstract

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