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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 252 Documents
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Keragaman Genetika Empat Belas Aksesi Kentang (Solanum tuberosum L.) Berdasarkan Marka SSR dan STS (Genetic Diversity of Fourteen Potato Accessions Based on SSR and STS Markers) Nugroho, Kristianto; Reflinur, Reflinur; Lestari, Puji; Rosdianti, Ida; Terryana, Rerenstradika T.; Kusmana, Kusmana; Tasma, I Made
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p41-48

Abstract

Potato is one of high economically horticultural plant. The increasing of national consumption of potato becomes a challenge forpotato breeders. The success of breeding programs is depending on availability of genetic diversity. The aim of this research wasto analyze the genetic diversity of fourteen accessions of potato by using SSR and STS markers. PCR analysis was scored as binerdata and the collected data was analyzed using NTSYS and PowerMarker. The result showed that there were 63% polymorphic(12 markers) of total markers. As many as 60 alleles with the size of 200–500 bp were identified by a range of 2–9 alleles perlocus. The polymorphism level was 0.59 (0.36–0.74). Result also showed the average of major allele frequency was 49.42%(35.71–63.64%). Nine markers which have polymorphism level more than 0.5 could be used to detect genetic diversity of potato.The average of genetic diversity index was 0.65. Cluster analysis showed that 14 accessions of potato were split in two groups(coefficient 0.70). The first groups consisted of Atlantik, GM 05, Granola Kembang Merbabu 17, and the second groups consist ofRepita, Maglia, Medians, CIP397078.7, CIP392781.1, Margahayu, Granola, CIP394613.139, Amabile, and Tenggo. The informationof genetic diversity of this germplasm could be used as a preliminary basis for choosing crossing parents in potato breeding inIndonesia.
Purifikasi dan Karakterisasi α-amilase Termostabil dari Bacillus stearothermophilus TII-12 Lestari, Puji; Richana, Nur; Darwis, Abdul Aziz; Syamsu, Khaswar; Murdiyatmo, Untung
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p56-62

Abstract

Purification and Characterization of Thermostableα-amylase from Bacillus stearothermophilus TII-12. PujiLestari, Nur Richana, Abdul A. Darwis, Khaswar Syamsu,and Untung Murdiyatmo. Thermostable α-amylase is apotential enzyme employed in the starch processing andwidely used in food industries, but this enzyme is stillimported. The local enzyme production would be moreeconomist and useful for its broad applications. Here wereport α-amylase from indigenous bacteria TII-12 which waspurified and characterized, as well as analyzed its hydrolysisproduct on cassava starch. The enzyme of Bacillusstearothermophilus TII-12 partially purified by ultrafiltration,acetone precipitation and gel filtration (Sephadex G-100)showed the reduced total activity, total protein and yield, butincreased the specific activity. The enzyme had a Km of 1,06mg/ml and Vmax of 1,21 mol/min, with optimal activity at pH 7and 90oC. An apparent molecular mass was of 192.932,8Dalton, as estimated by Native-Polyacrylamide Agarose Gelelectrophoresis. Its activity was inhibited by the divalentcation chelator such as EDTA and CuSO4 but activated bycalcium ion. Hydrolysis products of this enzyme on cassavastarch were glucose, dextrin, maltose and oligosaccharides.After 24 hours of hydrolysis, the concentration of glucoseand maltose reached 51.970 and 10.090 ppm, respectively.The thermostable α-amylase of TII-12 is an endo-α-amylaseand prospective to be applied on starch liquefaction withhigh temperature process.
Analisis Keragaman Genetik 161 Aksesi Mangga Indonesia Menggunakan Marka Mikrosatelit Tasliah, Tasliah; Rijzaani, Habib; Hariyadi, Tri Z. P.; Yuriyah, Siti; Rebin, Rebin; Ma; Silitonga, Tiur S.
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p125-134

Abstract

Mango is one of the fiveimportant fruit crops in the world. Microsatellite markers canbe used to analyze genetic diversity among mangoaccessions. The purpose of this research was to determinethe relationship among mango germplasm collection usingmicrosatellite markers. A total of 161 mango accessionsoriginated from Indonesian Tropical Fruit Research Institute(Cukurgondang Field Station), Pasuruan, East Java, wereused in this research. Twenty-six microsatellite markerswere used to genotype each accession. Genotyping wasconducted using Beckman Coulter® CEQ™ 8000 machine.Genetic relationship among accecions was analyzed usingthe Unweighted Pair Group Method with Arithmetic Mean(UPGMA) method, followed by bootstrap analysis. The resultshowed that high allele variation (15-75 alleles) wasobserved among mango accesions tested, with an averageallele number of 38.69. The average of PolymophismInformation Content (PIC) value was 0.548 (0.021-0.949).Fifteen microsatellite markers showed PIC value >0.5indicated that these markers were suitable for mangodiversity studies. Cluster analysis divided the mangocollections into two groups. Group I consisted of 95accessions, and group II consisted of 66 accessions. NinetyIndonesian indigenous mangos (84.11% of Indonesianmango accessions) could be separated from the introducedaccessions.
Keterpautan 23 Marka Mikrosatelit pada Kromosom 6 dan 7 dengan Karakter Ketahanan Populasi Jagung terhadap Penyakit Bulai (Peronosclerospora maydis) Roberdi, Roberdi S; Aswidinnoor, Hajrial S; Setiawan, Asep S; Sutrisno, Sutrisno S; Pabendon, Marcia B; Azrai, Muhammad S
Jurnal AgroBiogen Vol 6, No 1 (2010): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n1.2010.p10-17

Abstract

Linkage of 23 Microsatellite Marker on Chromosome 6and 7 to Downy Mildew Resistance on Maize. Roberdi,Hajrial Aswidinnoor, Asep Setiawan, Sutrisno, Marcia B.Pabendon, and M. Azrai. Downy mildew caused byPeronosclerospora is one of most important maize diseasein several countries, including Indonesia. Parental andprogenies selection based on conventional breeding is timeconsuming and laborious. Development of molecularbiology produces many DNA markers used for selection, oneof them is microsatellite. The aim of this research to identifymicrosatellite markers associated with downy mildewresistance on maize progeny MR-4 X AMATLCOHS-9-1-1-1-1-1-2-B, on chromosome 6 and 7. This research was consistedof two activities, phenotypic and genotypic analysis.Phenotypic analysis used 175 progenies BC1F2 and both ofparents. This analysis included planting of spreading row,inoculums preparation, inoculation of spreader rows, testmaterial planting, inoculation of test material andobservation. Genotypic analysis used 175 progenies BC1F1and both of parents. This analysis included DNA genomeisolation, PCR analysis, electrophoresis, gel staining and datascoring. Percentage of downy mildew infections on MR-4was 76%, while these on AMATLCOHS-9-1-1-1-1-1-2-B was16%, and on 175 progenies had range from 10.1-100%. Out of23 SSR, 12 markers could be mapped in chromosome 6 and11 markers in chromosome 7. QTL analyses showed thatchromosome 7 contain one QTL in position between phi082and phi116I marker as far as 18.6 cM with 2.6 LOD value.
Isolasi dan Purifikasi Inhibitor α-amilase dari Biji Kacang Phaseolus vulgaris Husin, Bahagiawati A.
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p7-12

Abstract

Plant genetic engineering technologies enable of introducing insect resistance genes into crop plants. The cry genes, genes encoding for inhibitor of digestive enzymes of a target insect that were isolated from Bacillus thuringiensis can be used for this purpose. Seeds of common bean (Phaseolus vulgaris) contain a glycoprotein that inhibits activity of α-amylase of insects. A α-amylase inhibitor was purified from the common bean seeds. The purified α-amylase inhibitor was then fed to cowpea storage weevil, Callosobruchus maculatus. The results showed a lengthened larval development time inside the seed and caused mortality to the insect larvae. This experiment suggests that the α-amylase inhibitor gene from the common bean seed could be used as a candidate gene for genetic engineering of plant resistance to bruchid insects.
Keragaman Genetik 96 Aksesi Plasma Nutfah Padi Berdasarkan 30 Marka SSR Terpaut Gen Pengatur Waktu Pembungaan (HD Genes) Utami, Dwinita Wikan; Sutoro, Sutoro; Hidayatun, Nurul; Risliawati, Andari; Hanarida, Ida
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p76-84

Abstract

Genetic Diversity of 96 Accession of Rice GermplasmUsing 30 SSR Markers Linked to Heading Date Genes (HDGenes). Dwinita W. Utami, Sutoro, Nurul Hidayatun,Andari Risliawati, and Ida Hanarida. Rice with earlymaturity is one of an important genetic resources in ricegermplasm collection. Characterization and identification ofgenetic diversity is an important issue for plant variety protection.Molecular identification by microsatellite markersusing Genetic Analyzer enables resolve of this issue. Theobjective of this research is to identify the genetic diversity of96 rice accessions based on their specific DNA fingerprintusing microsatellite markers. A total of 96 accessions consistingof a diverse variety of maturity classification weregenotyped with 30 SSR markers linked to HD genes whichspread out in 12 chromosomes of rice geneome. The total297 alleles were detected indicated the level of markerinformativeness. RM5607 generated 7 allele with the sizerange from 103 to 197 and the highest PIC at 0.90. RM3571(linked to HD12 gene) has a significant value associated withvarieties which have early maturity trait. Clustering analysisshowed the cluster based on Sub Species genome backgroundand on early maturity trait.
Perkembangan Aplikasi Teknik Kriopreservasi untuk Konservasi dan Mendukung Program Pemuliaan Tanaman Roostika, Ika
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p39-48

Abstract

In the beginning, cryopreservationtechnique was primarily used for germplasmslong-term storage as passive collection because cell divisionand metabolism process can be stopped at super lowtemperature, commonly in liquid nitrogen. The technique issuitable for vegetative propagated plants and recalcitrantseeds. Recently, its application is extending for storing manyspecies and orthodox seeds. In this paper, the developmentof cryopreservation application is discussed. In Indonesia,cryopreservation is being studied but the application ofcryopreservation has been significantly developed abroad.The application of cryopreservation technique is not only forpreserving passive collections but also for storing activecollections, including to provide plant materials for hybridization,cellular engineering, genetic transformation, as wellas pathogens eradication or cryotherapy. It is concluded thatcryopreservation plays an important role in conventional andmodern plant breeding program.
Analisis Sidik Jari DNA Plasma Nutfah Kedelai Menggunakan Markah SSR Santoso, Tri Joko; Utami, Dwinita Wikan; Septiningsih, Endang M.
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p1-7

Abstract

Accuracy is an important issue for plant germplasm identification, especially forvarietal conformation, registration, and plant protection. A study was conducted to determine genetic variation in 96 soybean accessions based on variation in size and number alelles using fluorescently-labeled SSR (Simple Sequence Repeat) markers on a capillary-electrophoresis DNA analyzer. This technology can be used to measure sizes of DNA fragments accurately and the genotyping protocol can be automated in a high-throughput manner. In addition, the germplasm as a whole can be further analyzed to measure the amount of genetic diversity and to identify agronomically-important genes or alleles for variety improvement. Results of the study indicated that nearly all the soyben accessions tested showed unique DNA fingerprints or genetic identities. The rare alleles (frequency <5%) that might have the potential in the variety improvement program had also been detected. Identification of the 96 soybean accessions using 10 SSR markers had detected 116 alleles, ranging between 7-19 alleles per locus, with the value of PIC (Polymorphism Information Content), reflecting the value of frequency and allele variation) 0.703. The tendency for clustering together of the allelles in certain groups of the improved soyben varieties indicating that there were close genetic relationships among them. In addition, molecular differences between two accessions having the same names but with different number of registrations were detected. Furthermore, the presence of two soybean accessions with different names but having the same molecular identity was also identified.
Efisiensi Mikropropagasi Pisang Kepok Amorang melalui Modifikasi Formula Media dan Temperatur Supriati, Yati
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p91-100

Abstract

Micropropagation Efficiency of Banana cv KepokAmorang through Modifications of Culture Media andIncubation Temperature. Yati Supriati. The budlessbanana cv Kepok Amorang is potentially commercializeddue to its sweet taste and does not have flower bud, hencereduced the potential of being infected by the blood diseasepathogen. Enhancement of banana industry needs continuoussupplies of large number banana seedlings. In vitroculture enable the production of seedlings in a large scale,uniform, quick. The research aims: (1) to formulate anefficient medium for in vitro multiplication of cv KepokAmorang shoot, (2) to identify efficient growth environmentfor in vitro culture of cv Kepok Amorang, and (3) to formulatean efficient culture medium for roots inductions of cvKepok Amorang. The plant material used was in vitro cultureof Kepok cv Amorang, 2 cm in height without leaf and root.The media formulation for shoot multiplication were fullstrength, half strength, one fourth strength MS media,supplemented with either 1, 3, or 5 ppm IBA. On optimizationstep, the media tested were MS, Knop, Knop andHeller, Hyponex N, Growmore N, and Rosasol N containingof 1 ppm BA. The explants were incubated in culture roomwith 8, 12, and 16 hours photoperiod with temperatures 30oC(non air conditioned) and 25oC (air conditioned). The rootinduction trial was done using MS, Knop, Knop and Heller,Hyponex N, Growmore N, and Rosasol N media containingof 1 ppm and 3 ppm IBA. The results showed that the bestmedium formula for shoot multiplication was ¼ MS + 1 ppmIBA. The best incubation condition was 16 hours photoperiodsat 30oC. The best media for root induction wasHyponex 2 g/l + 1 ppm IBA. This culture method reducedcost by Rp 261.7 per plantlet through efficiency of mediaformulation and electricity use.
Identifikasi dan Aplikasi Marka Berbasis PCR untuk Identifikasi Varietas Padi dengan Palatabilitas Tinggi Lestari, Puji; Risliawati, Andari; Koh, Hee Jong
Jurnal AgroBiogen Vol 8, No 2 (2012): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n2.2012.p69-77

Abstract

To date, there hasbeen no DNA fingerprint profile as unique identity of ricevariety that has a high palatability (overall eating quality) inIndonesia, thus identification of premium varieties usingmolecular markers is considered to be important. This studyaimed to establish DNA fingerprint profiles of indica andjaponica rice varieties, and unique identities of rice varietieswith high palatability using molecular markers associatedwith palatability. Total of 22 japonica and 24 indica ricevarieties were evaluated their overall eating quality andtested using 20 molecular markers STS (sequence-taggedsite) which were designed on the basis of japonica ricegenome. To identify the genes functions, all these markersamplicons were cloned, transformed, sequenced and thesequences results were analyzed their homologous againstthe genome database. Ilpum (japonica) and Rojolele(indica) were identified to have the highest palatabilitycompared to other varieties. DNA fingerprint profilesidentified with the total STS markers were not able todifferentiate each variety, however premium varieties ofjaponica and indica showed specific identities. A uniqueidentity of Indonesian indica variety possessing highpalatability, Rojolele was successfully developed using amarkers set. DNA fingerprint profile in digital value systemfacilitates the identification of premium rice from othervarieties. The fragments of the STS primers showed no anyknown-genes functions related to rice eating quality,therefore these markers are preferentially used foridentification of premium rice with high palatability thandifferentiation of rice varieties based on the palatability. Inthis study, the unique identity of rice variety with highpalatability is very usefull to evaluate the purity forgermplasm protection.

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