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INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 252 Documents
 10   11   12   13   14 
Parameter Genetik Jagung Populasi Bisma pada Pemupukan yang Berbeda. II. Ragam dan Korelasi Genetik Karakter Sekunder Sutoro, Sutoro; Bari, Abdul; Subandi, Subandi; Yahya, Sudirman
Jurnal AgroBiogen Vol 3, No 1 (2007): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v3n1.2007.p9-14

Abstract

Genetic Parameter of Secondary Traits of Corn in BismaPopulation under Different Fertilizer Application. II. GeneticVariance and Correlation of Secondary Traits.Sutoro, Abdul Bari, Subandi, and Sudirman Yahya. Themagnitute of secondary traits of corn could affect the cornyield. Genetic parameter value of secondary traits areimportant in plant breeding, especially in selection program.Genetic parameter could be used for estimation of correlatedresponse by involving value of genetic correlation andgenetic variance. Value of genetic parameter is differentamong population and environment. Genetic parameter ofsecondary traits on Bisma population under 3 different levelof fertilizer application were studied in Bogor. Result of thestudy showed that addtive genetic variance of ASI, chlorofil,seed number, amount of green leaves and LAI at floweringstage greater than dominant variance. Conversely, additivegenetic variance of green leaf number and LAI at maturingstage, and leaf senescene are lower than dominant variance.ASI has greatest heritability among secondary traitsunder three level of fertilizer application. Positive geneticcorrelation was found between grain yield under low fertileizerapplication and ASI under high level fertilizer applicationor between grain yield under high level of fertilizer applicationand ASI under low level fertilizer application.
Multiplikasi Tunas dan Induksi Perakaran pada Ubi Kelapa (Dioscorea alata L.) dan Gembili (Dioscorea esculenta L.) Secara In Vitro Hutami, Sri; Purnamaningsih, Ragapadmi; Mariska, Ika; Diantina, Surya
Jurnal AgroBiogen Vol 10, No 2 (2014): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v10n2.2014.p53-60

Abstract

Dioscorea sp. (yam) is one of the minor tuber crops whichgrows wildly in the forest and only a few of its species arecultivated and used as main or secondary food.Conservation is needed to preserve plant genetic material.The objective of this research was to obtain methods ofplantlets propagation of D. alata L. and D. esculenta L.through in vitro culture. The research was conducted atTissue Culture Laboratory of ICABIOGRAD in 2012. Theresearch consisted of three stages. First, shoot emergence.In this experiment, young shoots were planted in MS basicmedium combined with benzyl adenine (BA) (0, 1, 3, and 5mg/l) and gibberelic acid (GA) (0 and 5 mg/l). Second, shootmultiplication. Shoots of Dioscorea which were planted inthe best medium of the first experiment were subcultured inMS medium combined with thidiazuron (0, 0.1, 0.5, 1, 2, and3 mg/l). Third, root initiation. Shoots of Dioscorea whichwere planted in the best medium of the second experimentwere subcultured in MS medium (½ MS and 1 MS)combined with indole-3-butyric acid (IBA) (0, 1, 3, and 5mg/l). Result of these experiments showed that shootemergence of D. alata L. and D. esculenta L. began at 2weeks after planting in MS medium. More plantlets of D.alata L. and D. esculenta L. were obtained by shootmultiplication in MS media. Root initiation of the Dioscoreabegan at 4 weeks after planting in MS media. The addition ofIBA (3–5 mg/l) on D. esculenta L. could not stimulate rootingbut led to the formation of callus at the base of the stembuds.
Status Perkembangan Perbaikan Sifat Genetik Padi Menggunakan Transformasi Agrobacterium Rahmawati, Syamsidah
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p36-44

Abstract

Genetic transformation of rice becomes an important research area in recent years. Rice is staple food for almost half of world population and has been extensively used as a plant model system for monocotyledonous plant. Compare to direct DNA transfer techniques (PEG, electroporation, and DNA bombardment), Agrobacterium tumefaciens-mediated transformation was considered to be more advantageous because it is easy to handle, integration and segregation pattern are more predictable, and the likelihood to get transgenic plant with low copy number is high, thus decreasing gene silencing phenomena. Various important genes have been introduced into rice genome via Agrobacterium transformation. A number of important factors affecting the Agrobacterium transformation and the application of this technique in the next future will be discussed.
Analisis Integrasi dan Segregasi Gen Ketahanan terhadap Hawar Daun pada Progeni F1 Hasil Persilangan Tanaman Kentang Transgenik dengan Non Transgenik Ambarwati, Alberta Dinar; Purwito, Agus; Herman, Muhamad; Sumaraow, S. M.; Aswidinnoor, Hajrial
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p%p

Abstract

Potato late blight, caused by Phytophthora infestans is one of the most devastating plant diseases. Potato yield losses due to this disease ranged from 47-100%. Frequent intervals and high rates of fungicide spray, currently practiced by potato growers to control the disease are expensive. Host resistance is an alternative control measure that is more economically and environmentally sustainable. Development of late blight resistant plants was conducted by crossing RB transgenic Katahdin SP904 andSP951 as male and two susceptible (Atlantic, Granola) varieties as female parents. F1 progenies were molecularly characterized for the integration of the RB transgene and evaluated for their segregations. Crossing data of Atlantic x transgenic Katahdin SP904 and SP951 produced 71 (57.72%) berries with average number of seeds per berry 139.58 and 83 (41.29%) berries with average number of seeds/berry 85.23, respectively. Granola x transgenic Katahdin SP904 and SP951 crosses gave higher results in terms of berry set (79.55 and 84.44%, respectively) than Atlantic x transgenic Katahdin crosses. A total of 554 F1 progenies were analyzed for the presence of the RB PCR marker. An expected 619-bp and 840-bp band were amplified in the progenies that contain the RB gene. The RB gene was integrated in 65 (45.45%), 77 (47.83%), 47 (45.63%), and 71 (48.30%) F1 progenies of Atlantic x transgenic Katahdin SP904, Atlantic x transgenic Katahdin SP951, Granola x transgenic Katahdin SP904, andGranola x transgenic Katahdin SP951, respectively. Chisquare tests showed that all the four cross combinations followed a 1 : 1 segregation ratio.
Identifikasi cDNA Gen RB pada Tanaman Kentang Produk Rekayasa Genetika Katahdin SP951 Hadiarto, Toto; Listanto, Edy; Riyanti, Eny I.
Jurnal AgroBiogen Vol 11, No 2 (2015): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n2.2015.p59-64

Abstract

To overcome the negative impact of the fungicide application, RB gene, originated from Solanum bulbocastanum, a resistancegene to the disease has been introduced to the genome of potato plant Katahdin, which was later named as Katahdin SP951.The aims of the research were to confirm the RB gene in the genetically modified plant (GMP) Katahdin SP951 throughresequencing the RB gene in the Katahdin SP951 genome and to align the RB genes isolated from the transgenic Katahdin SP951and the cloning vector pLCD0454, which was used the vector to transform Katahdin cultivar. The RB gene has high similarity toother resistance genes in potato plants, and therefore nontransgenic Katahdin, and Solanum bulbocastanum were used in theanalysis as the negative and positive control, respectively. Seven pairs of specific primers, which could differentiate the RB genewere used for gene sequencing studies. The sequencing experiments obtained 2913 bases which showed 100% similarity to theRB sequence isolated from the pLCD04541 plasmid. It is concluded that the RB gene in the Katahdin SP951 did not experiencemutation during transformation process and integration into the Katahdin genome.
Keragaman Genetik Beberapa Genotipe Jarak Pagar (Jatropha curcas L.) Berdaya Hasil Tinggi Berdasarkan Karakter Morfologi, Agronomi, dan Isozim Dewi, Iswari S; Arisanti, Yusie; Purwoko, Bambang S.; Hariyadi, Hariyadi; Syukur, M.
Jurnal AgroBiogen Vol 9, No 1 (2013): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n1.2013.p28-38

Abstract

The interest in using Jatropha curcas L. from thefamily Euphorbiaceae for the production of biofuel is rapidlygrowing. The research objective was to determine geneticvariation of several high yielding physic nuts based on theirmorphology, agronomy, and isozyme characters. Theresearch used Completely Randomized Design with threereplications. The treatment was consisted of 8 genotypes i.e.IP-1A, IP-1M, IP-2P, Lombok Timur, Lombok Tengah,Lombok Barat, Sumbawa, and Bima. Analysis of isozyme ofthe eight genotypes was also conducted according to 5enzyme systems, i.e. peroksidase, esterase, aspartataminotransferase, malat dehidrogenase, and alcoholdehidrogenase. Observation was done on qualitative andquantitative characters as well as banding pattern derivedisozymes.The results showed that genetic variation was lowwhen based on qualitative characters and isozyme (0-25%)but relatively high when based on selected quantitativecharacters analysis (17-81%). Analysis of combinedqualitative, quantitative, and isozyme characters still gavelow genetic variation (6-33%). Based on the quantitativecharacters at similarity coefficient of 46% the genotypes canbe devided into three clusters. Improved populationgenotypes, i.e. IP-1A, IP-1M, and IP-2P were placed in 3different clusters, while other genotypes from NTB areawere grouped in the same cluster. Therefore, selectionamong population of the same ecotype based on agronomiccharacters such as yield components, yield and oil contentwas suitable in Jatropha improvement, especially whengenetic variation was low. Furthermore, introduction,mutation and crossing are suggested to increase geneticvariation of current Jatropha collection.
Analisis Regulasi dan Kebijakan Keamanan Hayati dan Peluang Keberhasilan Adopsi Benih Transgenik di Indonesia Saragih, Edwin S; Sitorus, Santun RP; Harianto, Harianto; Moeljopawiro, Sugiono
Jurnal AgroBiogen Vol 6, No 1 (2010): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n1.2010.p40-48

Abstract

Analysis of Regulation and Policy on Biosafety andLikelihood of Transgenic Seeds Adoption in Indonesia.Edwin S. Saragih, Santun R.P. Sitorus, Harianto, andSugiono Moeljopawiro. Since more than 10 years, anumber of works in field of modern biotechnology havebeen programmed in public research institutes anduniversities in Indonesia and few foreign companies haveput efforts in introducing transgenic varieties. This significantdevelopment raises intriguing question as to why there hasnot been any transgenic food crop seed planted by farmersin the country. A status quo was observed in whichregulatory regime on biosafety has been in a situation ofprolonged transitional phase and necessary institutionalframework has not been firmly in place. There weredistinguished lines among stakeholders on benefitawareness, risks perception and worry on multinationalcompanies’ control over seed supply. There is a fairexpectation that similar benefits experienced by adoptingcountries could also help increase food production inIndonesia. However, potential contribution of transgenicseeds for the country is still largely unexplored. There arenumbers of potential transgenic seeds namely transgenicrice, soybean, potato, tomato and corn, with the latter wouldshow slightly better likelihood of success once adoptionhappens. Decision making instrument as determinant factorin ensuring safe application and release of transgenic seedshas not yet existed despite the fact that capacity for biosafetyassessment conduct is undoubtedly sufficient. It is importantto note that the new regulation on biosafety (PP No. 21/2005)open opportunities for assessing transgenic product under atransitional clause. Nonetheless, the new regulation has notbeen able securing food safety statement of importedtransgenic products (especially corn and soybean) whichhave been used for domestic consumption.
Penyisipan Gen Inhibitor α-amilase pada Plasmid Biner pCambia 1301 Listanto, Edy; Sutrisno, Sutrisno; Pardal, Saptowo J.; Herman, M.
Jurnal AgroBiogen Vol 1, No 2 (2005): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n2.2005.p45-52

Abstract

The experiment was conducted at the Molecular Biology Laboratory of the Indonesian Center for Agricultural Biotechnology and Genetic Resources Research and Development, Bogor. The objective was to construct -ai gene on a binary plasmid pCambia 1301. This experiment was carried out using construction method by ligation process between fragments of α-ai gene from pTA3 plasmid and pCambia 1301 on HindIII site. The result of ligant transformation into E. coli DH5α was 182 surviving colonies on YEP medium containing kanamycin. DNA samples were obtained from 60 randomly selected colonies. The restriction pattern was tested by digesting each DNA sample using HindIII showed colonies containing two fragments expected of sizes wich are 11.837 and 4.887 kb. Two colonies are predicted containing of α-ai gene on its the binary plasmid. Advanced tests using restriction enzymes BamHI and XbaI showed two directions (right and left) of α-ai gene. The right direction was shown by pCambia-α-ai1 from colony number 43. This plasmid showed expected fragments of sizes 13.485 and 3.219 kb when digested with BamHI and two fragments of sizes 15.421 and 1.303 kb when digested with XbaI. The left direction was shown pCambia-α-ai2 from colony number 58. This plasmid also demon-strated expected fragments of sizes 15.026 and 1.698 kb when digested with BamHI and two fragments of sizes 13.082 and 3.642 kb when digested with XbaI. Both pCambia-α-ai1 and pCambia-α-ai2 were transformed into A. tumefaciens LBA4404.
Pengaruh Sumber Karbon dan Kondisi Inkubasi terhadap Pertumbuhan Kultur In Vitro Purwoceng (Pimpinella pruatjan Molk.) Roostika, Ika; Purnamaningsih, Ragapadmi; Noviati, Arief V.
Jurnal AgroBiogen Vol 4, No 2 (2008): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v4n2.2008.p65-69

Abstract

Pruatjan (Pimpinella pruatjan Molk.) is an Indonesianmedicinal plant which is categorized as endangeredplant and included in Appendix I based on CITES. The invitro conservation techniques have been studied. However,the storage period was very short (4 months) when plantgrowth retardant and media dilution were applied. Besidethat, the residual effect of growth retardant was strongenough so that it needed more than 4 months for recovery.Thus, the use of certain carbon source may prolong thepreservation period with shorter time for recovery. Theobjective of the study was to know the effects of carbonsources (sucrose and mannitol) and culture conditions (cultureroom and growth chamber) to the growth of pruatjancultures. This application was hoped to prolong preservationperiod of pruatjan longer than 4 months and to cut therecovery period after presservation. The study was conductedat Tissue Culture Laboratory in Indonesian Center forAgricultural Biotechnology and Genetic Resources Researchand Development from August 2006 to July 2007. Theactivities included propagation of in vitro shoot grown invitro as explants source, preservation of in vitro shoots ofpruatjan, and regeneration of the cultures after preservation.The experiment was designed as factorial in RandomizedCompletely Block Design with 6 replications. The DKW basalmedia containing 1 ppm BA, 0.2 ppm thidiazuron, and 100ppm arginine were supplemented with mannitol or sucroseat the level of 1, 2, 3, 4, and 5%. The observed variables weretotal number of leaves, number of shoot, and number of wiltleaves. The result revealed that pruatjan cultures could bestored longer than 4 months. Generally, the effect ofmannitol or sucrose was more dominant than that of culturescondition. The mannitol (1-5%) strongly inhibited thegrowth of pruatjan cultures so that only a few culturessurvived at 7 months preservation period and needed about1 month for recovery. On the contrary, the effect of sucrose(at the same level) was better than mannitol. The 2.5%sucrose optimally inhibited pruatjan cultures. At that condition,the cultures could be stored for 10 months withoutmorphological changes so that they could recover spontaneously.
Nodulation and Growth of a Supernodulating Soybean Mutant SS2-2 Symbiotically Associated with Bradyrhizobium japonicum Lestari, Puji; Van, Kyujung; Kim, Moon Young; Lee, Suk-Ha
Jurnal AgroBiogen Vol 2, No 1 (2006): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v2n1.2006.p8-15

Abstract

Mutan kedelai penghasil nodulsuper menunjukkan kelemahan dalam kontrol autoregulasi pada nodulasi dan perbedaan fenotip dibandingkan dengan tipe liarnya. Studi untuk mengevaluasi karakter pertumbuhan dan nodulasi dari kedelai penghasil nodulsuper dalam asosiasinya dengan Bradyrhizobium japonicum dilakukan dalam penelitian ini. Tiga genotip kedelai, yaitu mutan kedelai penghasil nodulsuper SS2-2, tipe liarnya Sinpaldalkong 2 dan kedelai kontrol Jangyeobkong diinokulasi dengan B. japonicum USDA 110, kemudian ditumbuhkan di rumah kaca dalam kondisi terkontrol. Karakter nodulasi, fiksasi nitrogen (Acetylene Reduction Activity/ARA), pertumbuhan tanaman, dan hasil biji ditentukan untuk mengevaluasi asosiasi simbiotik antara B. japonicum dan kedelai nodulsuper. Kedelai yang diinokulasi dengan B. japonicum menunjukkan peningkatan jumlah dan berat kering nodul serta berat kering total tanaman dibandingkan dengan tanpa inokulasi. Tanaman SS2-2 yang diinokulasi menunjukkan jumlah nodul sekitar 20 kali lipat lebih tinggi daripada tipe liarnya. Inokulasi B. japonicum ternyata juga meningkatkan fiksasi nitrogen seiring dengan perkembangan nodul. Tanaman S2-2 lebih pendek dan menghasilkan fiksasi nitrogen (ARA) lebih tinggi, tetapi spesifik ARA dan hasil biji lebih rendah dibandingkan dengan tipe liarnya. Berdasarkan hasil evaluasi terhadap nodulasi dan pertumbuhannya, interaksi Rhizobium dan kedelai penghasil nodulsuper SS2-2 mempunyai respon asosiasi simbiotik lebih rendah dibandingkan kedelai penghasil nodul normal (kedelai yang tidak mendapat perlakuan mutasi).

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