cover
Article Per Year (5 Year)
All
Home Page
OAI Link
Editorial Team
Contact
Reviewer
Google Scholar
Contact Name
-
Contact Email
-
Phone
-
Journal Mail Official
-
Editorial Address
-
Location
Kota adm. jakarta selatan,
Dki jakarta
INDONESIA
Jurnal AgroBiogen
Published by Kementerian Pertanian
ISSN : 19071094     EISSN : 25491547     DOI : -
Core Subject : Agriculture,
Agriculture, Biological Sciences & Forestry
Jurnal AgroBiogen memuat artikel primer dan sekunder hasil penelitian bioteknologi dan sumberdaya genetik tanaman, serangga, dan mikroba pertanian. Jurnal ini diterbitkan tiga kali setahun pada bulan April, Agustus dan Oktober oleh Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumberdaya Genetik Pertanian
Arjuna Subject : -
Articles 252 Documents
 12   13   14   15   16 
Transformasi Genetik Pisang Ambon dengan Gen Kitinase dari Padi Ragapadmi Purnamaningsih; Deden Sukmadjaja
Jurnal AgroBiogen Vol 8, No 3 (2012): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v8n3.2012.p97-104

Abstract

One of the main constrains on theproductivity and quality enhancement of banana is wiltdiseases caused by Fusarium oxysporum (Foc). Productiondecrease by wilt disease was 63.33%. Therefore, an effort toobtain the banana new variety which is tolerant to fusariumwas absolutely necessary to be done. Genetic engineeringcan be used in new variety improvement, especially forproduction of pest and disease tolerant varieties.Transformation of banana with chi gene which expressedchitinase enzyme have been used in obtaining the plantresistant to Foc. The goals of the research were to obtain:determine lowest higromisin consentration inhibited nodulgrowth by tested four consentration of higromisin,determine optimum cocultivation time by tested three timescocultivation, tested asetosiringone added on two timescocultivation, and gen chi introduction at bananatransforman shoots with PCR. The explants used werenodule induced from pseudostem of banana cv. Ambonkuning. Genetic transformation done by sowing the explantsin bacterial suspension 0, 15, 30, and 45 minutes.The effectof asetosiringone (0 and 100 mg/l) on cocultivation mediumwas observed. The research results showed that the lowesthigromisin concentration inhibited nodule growth was 25mg/l for 5 weeks and the best time for inoculation of nodulewere 30 minute. Asetosiringone added on bacterialsuspension did not increase transformation efficiency.Chitinase gene transformation using Agrobacteriumtumefaciens on banana nodules produced 25 noduly ines ofputative transformant on selection media and 34 plantstransforman identification by PCR.
Analisis Molekuler Gen Partenokarpi DefH9-RI-iaaM pada Progeni Tomat Transgenik Saptowo J Pardal; Slamet Slamet; Ragapadmi Purnamaningsih; Endang G. Lestari; Sutini Sutini
Jurnal AgroBiogen Vol 11, No 1 (2015): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v11n1.2015.p33-40

Abstract

The development of seedless tomato fruits will be more attractive to both consumers and industries. Seedless tomatoes can beproduced through parthenocarpy technology. Artificial parthenocarpy can be induced by conventional crossing, hormoneapplication, or genetic engineering. The development of parthenocarpic tomatoes through genetic engineering has been carriedout by inserting DefH9-iaaM parthenocarpic geneinto tomato genome via Agrobacterium tumefaciens mediated transformation.Sixty putative transgenic tomato lines were produced and three events (OvR1#14-4, OvM2#10-1, and OvM2#6-2) were selectedas the best events. The background of the tomato lines was Oval variety, and based on PCR results, the three selected linescontained DefH9-RI-iaaM in their genome. The objective of this research was to determine the integration of DefH9-RI-iaaMgene in the progenies of three transgenic tomatoes lines using PCR technique. The research was conducted in the laboratoryand Biosafety Containment Facility of Indonesian Center for Agricultural Biotechnology and Genetic Resources Research andDevelopment (ICABIOGRAD). Parental variety, Oval (neither transgenic nor in vitro cultured), and elite line of CL 6046 were usedas control plants. The results indicated that the progenies (T1, T2, and T3) of the three tomato lines contained the insert DefH9-RIiaaMgene.
Genetic Diversity Analysis of Jatropha Curcas Provenances Using Randomly Amplified Polymorphic DNA Markers Dani Satyawan; I Made Tasma
Jurnal AgroBiogen Vol 7, No 1 (2011): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n1.2011.p47-55

Abstract

Genetic Diversity Analysis of Jatropha CurcasProvenances Using Randomly Amplified PolymorphicDNA Markers. Dani Satyawan and I Made Tasma.Jatropha curcas nuts are rich in oil that is higly suitable forHak Cipta © 2011, BB-Biogenthe production of bio-diesel or to be used directly inmodified diesel engines. The objective of this study was toassess the extent of genetic diversity among 50 J. curcasprovenances and one accession of J. integerrima usingRAPD markers. The fifty J. curcas provenances werecollected from ecologically diverse regions of Indonesia, andplanted in the Pakuwon Experimental Station (Sukabumi,West Java). Fourteen RAPD primers with 60-80% G+Ccontent were used in this genetic diversity analysis andproduced 64 bands with 95.7% polymorphism level. ThePolymerase Chain Reactions used to generate the RAPDbands sometimes produced inconsistent and nonreproducibleresults, necessitating the duplication of eachreaction to prevent scoring errors. Sixty one validated bandswere subsequently used for genetic diversity analysis usingUnweighted Pair Group Method Arithmetic (UPGMA)method and Dice coefficients. It was shown that thesimilarity coefficients among the provenances ranged from0.2 to 0.98 with an average similarity of 0.75. Dendrogramanalysis produced two major groups of provenances, withone outlier from South Lampung. There was no tendency forprovenances originated from nearby regions to clustertogether in each group, and several provenances showedmore similarities with provenances originated from distantregions. This pattern lent credence to reports that Jatrophawas introduced to Indonesia around four centuries ago andwas mainly spread by humans. Based on the meansimilarities among the accessions and their clusteringpattern, the genetic diversity of the Jatropha collectionappeared to be fairly low. Future additions of geneticmaterials from more diverse genetic background will benecessary to maintain the current progress of Jatrophaimprovement program.
Pemuliaan Mutasi untuk Perbaikan terhadap Umur dan Produktivitas pada Kedelai Asadi Asadi
Jurnal AgroBiogen Vol 9, No 3 (2013): Desember
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n3.2013.p135-142

Abstract

To support the government policy inimproving soybean production, it is suggested to plant earlymaturing (<80 days), drought tolerant and high yieldingvarieties to be applied in the cropping system of rice-ricesoybeanand rice-rice-rice-soybean in lowland, and indryland cropping system of rice-soybean or rice-otherpalawija crops. Mutation breeding in soybean for earlymaturity and high productivity in soybean can be applied toobtain some new varieties. The breeding proceduresincluded selection using bulk method for M1 population,followed by the pedigree method for M2-M5 generations.Evaluation of uniformity (homozygous) of lines is done onthe M4 generation. Yield and adaption testing are conductedduring M5-M8 generations. Through mutation breeding earlymaturity soybean varieties were released elsewhere. Innational research institutes such as The National NuclearEnergy Agency (Batan) Indonesia, soybean mutation breedingactivities were begun since 1972, while in IndonesianCenter for Agricultural Biotechnology and GeneticResources Research and Development (ICABIOGRAD), itwas started in 2009. Batan has released two early maturingsoybean varieties through seed irradiation such as Tenggerin 1991 and Meratus in 1998. While in 2011 ICABIOGRADthrough irradiation of calli-derived embryo zygotic hasselected 50 early maturing and potentially yielding soybeanmutant lines. While through seed irradiation in 2012, 15soybean advanced lines that matured earlier anddemontrated higher yield were also obtained.
Introduksi Gen DefH9-iaaM dan DefH9-RI-iaaM ke dalam Genom Tanaman Tomat Menggunakan Vektor Agrobacterium tumefaciens Ragapadmi S Purnamaningsih
Jurnal AgroBiogen Vol 6, No 1 (2010): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n1.2010.p18-25

Abstract

Introduction of DefH9-iaaM and DefH9-RI-iaaM GeneInto Tomato Genome Using Agrobacterium tumefaciens.Ragapadmi Purnamaningsih. Plant genetic improvementcan be conducted through genetic engineering.Parthenocarpic fruit production could increase fruitproduction and its qulities. IAA genes were introduced intothree tomato cultivars Ratna, Opal and LV 6117 using twoconstract genes DefH9-iaaM and DefH9-RI-iaaM. The iaaMgene is able to increase auxin biosynthesis in transgenicplant cells and organs because indol-eacetamide,synthesized by the product of the iaaM gene, is convertedeither chemically or enzimatically to indole-3-acetic acid(IAA), while the promotor DefH9 enable IAA gene expressedspecifically in the ovules. The objectives of this experimentwas to identify gene introduction into plant genom of threetomato cultivars. The factors tested were two constract ofIAA genes (DefH9-iaaM or DefH9-RI-iaaM), tomato cultivars(Ratna, Opal, and LV 6117) and time of explant inoculation(5, 15, 30 minute). The result showed that the best timeinoculation was 5 minute. Otherwise three tomato cultivarsresponse better to DefH9-RI-iaaM than DefH9-iaaM. The totalefficiency of regeneration and total efficiency oftransformation of both genes were 25.38% and 20.32%. PCRanalysis showed that 10 plant have positive PCR, were 1plant carried (Opal) DefH9-iaaM gene and 9 plant (Ratna,Opal, LV 6117) carried DefH9-RI-iaaM gene.
Seleksi In Vitro Tanaman Lada untuk Ketahanan terhadap Penyakit Busuk Pangkal Batang Ali Husni; Mia Kosmiatin
Jurnal AgroBiogen Vol 1, No 1 (2005): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v1n1.2005.p13-19

Abstract

Root rot caused by Phytopthora capsici is one of the most important diseases of pepper, it can decrease yield up to 52%. Planting resistant plants is an efficient way to control the disease. In vitro selection is a method that can be utilized for selection of resistant plants. The objective of the study was to obtain pepper plants resistant to root rot disease through in vitro selection. The study consisted of four experiments, i.e., (1) induction of embryogenic calli, (2) production of filtrate of P. capsici, (3) in vitro selection and shoots regeneration, and (4) root induction. The results showed that leaf tissue was the best explants for production of embryogenic calli in a medium of Gamborg (macro nutrient) + MS (micro nutrients and vitamins) with 2.4-D 0.1 or 0.5 mg/l. The best filtrate of P. capsici culture used for the selection was that from the 5 day-old inoculum in the V8 medium. In general, addition of P. capsici culture filtrate into the regeneration media influenced the percentage of live calli. The addition of 50% and 75% of P. capsici filtrate was enabling to screening for adaptive calli based on brownish-yellow or yellowish-brown color. These calli produced 32 shoots in the regeneration media without the P. capsici filtrate. Root induction was successfully performed in the MS medium with 0.01 mg/l of NAA.
Plot Refugi untuk Pengelolaan Resistensi Hama terhadap Tanaman Transgenik Bt Bahagiawati Bahagiawati
Jurnal AgroBiogen Vol 7, No 2 (2011): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v7n2.2011.p128-137

Abstract

Refugea Plot as Insect Resistance Management inTransgenic Bt Crops. Bahagiawati. The objective of thisreview is to share information on several cases of targetinsects became resistance to transgenic-Bt crops in the fieldand the refuge strategy used to manage this problem. Btcorn and Bt cotton have been planted widely for severalyears globally. One of the risks of planting transgenic Bt cropis the ability of the target insects adapted to the Bt proteinand caused the resistance breakdown the transgenic Btplants. This phenomenon was hypothesized in early 1990sbased on the cases of several insects resistance to microbialBt sprayed in laboratories and in the field. The mode ofaction of the pest resistance to Bt-toxin have been studied inseveral laboratories. In USA, to avoid the target insectresistance to transgenic Bt crops, a program called InsectResistance Management (IRM) has been applied since 2001for farmers growing Bt crops. Lately, there have been somereports of target insects became resistance to cry1F, cry1Ab,and cry1Ac in transgenic Bt crops. A report informed aboutthe resistance of target insect in Puerto Rico was publishedin 2006, and so in South Africa in 2006/2007, and the last onein India in 2009. To avoid target’s insect become resistanceto Bt crops, a program called structural IRM and unstructuralIRM were introduced and applied in severalcountries. One of the components of IRM is planting refugeplot, a plot that planting with isogenic line of Bt crops in/nearby the area of Bt crops. This review will discuss about thecases of target insect became resistance to Bt crops in thefield, mode of action of insect resistance to Bt, the model ofIRM program in USA and the Philippines and finally therecommendation for Indonesia to prepare its IRM programfor implementing Bt crops.
Identifikasi Molekuler Hawar Daun Bakteri (Xanthomonas oryzae pv. oryzae) dan Uji Patogenisitasnya pada Galur-galur Padi Isogenik Tasliah Tasliah; Mahrup Mahrup; Joko Prasetiyono
Jurnal AgroBiogen Vol 9, No 2 (2013): Agustus
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v9n2.2013.p49-57

Abstract

Identification of Xanthomonas oryzae pv.oryzae (Xoo) based on molecular analysis has beenintroduced just few years ago. This method used somespecific primers for Xoo and can be done quickly. Thepurposes of this research were to identify isolate Xoooriginated from five locations in Indonesia and to determinethe level of pathogenicity of these bacteria. Studies wereconducted in the greenhouse and the Molecular BiologyLaboratory of ICABIOGRAD, from 2011 to 2012. Bacterialisolates were taken from five regions in Indonesia, namely:West Sumatra, West Java, Central Java, South Sulawesi, andWest Kalimantan. The specific primers of Xoo wereXoo2967, Xoo80, and Xoo. Results showed that 216 isolatescould be grown to form yellow colored colonies, whichbelongs to a criterian for Xoo. Molecular analysisdemonstrated that 189 isolates were Xoo and 27 isolateswere not. Amplification of DNA of the isolates resulted a 337bp PCR product for primer Xoo2976, 700 bp for primerXoo80 and 534 bp for primer Xoo. Pathogenicity tests of theXoo isolates showed xa5, Xa7, and Xa21 resistance geneswere still effective againts BLB pathogens originated fromthose five regions, with percentage of resistance were 93.57,77.49, and 85.37%, respectively.
Analisis Integrasi dan Segregasi Gen Ketahanan terhadap Hawar Daun pada Progeni F1 Hasil Persilangan Tanaman Kentang Transgenik dengan Non Transgenik Alberta Dinar Ambarwati; Agus Purwito; Muhamad Herman; S. M. Sumaraow; Hajrial Aswidinnoor
Jurnal AgroBiogen Vol 5, No 1 (2009): April
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v5n1.2009.p25-31

Abstract

Potato late blight, caused by Phytophthora infestans is one of the most devastating plant diseases. Potato yield losses due to this disease ranged from 47-100%. Frequent intervals and high rates of fungicide spray, currently practiced by potato growers to control the disease are expensive. Host resistance is an alternative control measure that is more economically and environmentally sustainable. Development of late blight resistant plants was conducted by crossing RB transgenic Katahdin SP904 andSP951 as male and two susceptible (Atlantic, Granola) varieties as female parents. F1 progenies were molecularly characterized for the integration of the RB transgene and evaluated for their segregations. Crossing data of Atlantic x transgenic Katahdin SP904 and SP951 produced 71 (57.72%)berries with average number of seeds per berry 139.58 and 83 (41.29%) berries with average number of seeds/berry 85.23, respectively. Granola x transgenic Katahdin SP904 and SP951 crosses gave higher results in terms of berry set (79.55 and 84.44%, respectively) than Atlantic x transgenic Katahdin crosses. A total of 554 F1 progenies were analyzed for thepresence of the RB PCR marker. An expected 619-bp and 840-bp band were amplified in the progenies that contain the RB gene. The RB gene was integrated in 65 (45.45%), 77 (47.83%), 47 (45.63%), and 71 (48.30%) F1 progenies of Atlantic x transgenic Katahdin SP904, Atlantic x transgenic Katahdin SP951, Granola x transgenic Katahdin SP904, andGranola x transgenic Katahdin SP951, respectively. Chisquare tests showed that all the four cross combinations followed a 1 : 1 segregation ratio.
Pengaruh Kinetin dan BAP terhadap Pertumbuhan dan Perkembangan Embrio Somatik Tanaman Sagu (Metroxylon sagu Rottb.) Imron Riyadi
Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober
Publisher : Balai Besar Penelitian dan Pengembangan Bioteknologi dan Sumber Daya Genetik Pertanian

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21082/jbio.v6n2.2010.p101-106

Abstract

Effect of Kinetin and BAP to Growth and Development ofSomatic Embryos of Sago Palm (Metroxylon sagu Rottb.).Imron Riyadi. Somatic embryos induction in sago palm(Metroxylon sagu Rottb.) have succesfully developed. Kindsand concentration of plant growth regulators (PGR’s)influence to growth and development of somatic embryos.The research was conducted to determine the optimal concentrationof BAP and kinetin for proliferation, maturationand germination of sago palm somatic embryos. Cotyledonstageof somatic embryo derived from shoot tip culturescultured on Modified Murashige-Skoog (MMS) with halfstrengthmacro-salts and added with 30 g/l sucrose, 2 g/lgelrite, 1 g/l activated charcoal. pH of media was adjusted at5.6 before sterilized. The media were supplemented with0.1-2.0 mg/l BAP and 0.1-2.0 mg/l kinetin in combination with0.01 mg/l ABA each for supporting growth and development.The cultures was incubated at 26+1oC under a 12-hphotoperiod with lighting providing an intensity 20 μmolesphotons/m2/second for 11 weeks with replication 10 times.The results showed that the highest of somatic embryoproliferation was achieved in a culture medium with BAP at0.5 mg/l + 0.01 mg/l ABA with an expression rate of 94%, thebest maturation at 1.0 mg/l kinetin + 0.01 mg/l ABA with anexpression rate of 93.5% and the most germination at 2.0mg/l kinetin + 0.01 mg/l ABA with an expression rate of100%. Transfer of these germinants to gelled media withoutPGR’s led to the development of normal plantlets.

Page 14 of 26 | Total Record : 252

 12   13   14   15   16 

Filter by Year

2005 2021


Reset
Filter By Issues
All Issue Vol 17, No 2 (2021): DESEMBER Vol 17, No 1 (2021): JUNE Vol 16, No 2 (2020): December Vol 16, No 1 (2020): June Vol 15, No 2 (2019): December Vol 15, No 1 (2019): June Vol 14, No 2 (2018): December Vol 14, No 1 (2018): June Vol 14, No 1 (2018): June Vol 13, No 2 (2017): Desember Vol 13, No 1 (2017): Juni Vol 12, No 2 (2016): Desember Vol 12, No 1 (2016): Juni Vol 11, No 3 (2015): Desember Vol 11, No 2 (2015): Agustus Vol 11, No 1 (2015): April Vol 10, No 3 (2014): Desember Vol 10, No 2 (2014): Agustus Vol 10, No 1 (2014): April Vol 9, No 3 (2013): Desember Vol 9, No 2 (2013): Agustus Vol 9, No 1 (2013): April Vol 8, No 3 (2012): Desember Vol 8, No 2 (2012): Agustus Vol 8, No 1 (2012): April Vol 8, No 1 (2012): April Vol 7, No 2 (2011): Oktober Vol 7, No 1 (2011): April Vol 7, No 1 (2011): Jurnal AgroBiogen Vol 6, No 2 (2010): Oktober Vol 6, No 1 (2010): April Vol 5, No 2 (2009): Oktober Vol 5, No 1 (2009): April Vol 4, No 2 (2008): Oktober Vol 4, No 1 (2008): April Vol 3, No 2 (2007): Oktober Vol 3, No 1 (2007): April Vol 2, No 2 (2006): Oktober Vol 2, No 1 (2006): April Vol 1, No 2 (2005): Oktober Vol 1, No 1 (2005): April More Issue