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Dinia R Dwijayanti,
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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
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Articles 6 Documents
 1 
Search results for , issue "Vol. 12 No. 1 (2024)" : 6 Documents clear
Isolation and Identification of Endophytic Bacteria from Kumis Kucing Leaves (Orthosiphon aristatus Benth.) Setiani, Nur Asni; Rafika Zahraeni; Siti Uswatun Hasanah
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.01

Abstract

Endophytic bacteria are in symbiosis with plants and have the potential to produce secondary metabolites similar to their host. Kumis kucing (Orthosiphon aristatus) is a traditional medicinal plant that has been proven to have many pharmacological activities, including antiviral and antibacterial. This study aims to isolate and characterize endophytic bacteria from kumis kucing leaves. Endophytic bacteria were isolated from kumis kucing leaf by spreading method on Trypticase Soy Agar (TSA) media and incubated for 24 hours at room temperature. Colonies that grew with bacterial morphology were inoculated on TSA media to obtain pure cultures. Pure cultures of isolates were identified through Gram staining, 16S rRNA gene sequencing, and bacterial growth curves. There were eight isolates with bacterial morphology, which were identified by Gram staining. The staining results showed that all were classified as Gram-negative with rod and coccus shapes. The 16S rRNA gene sequencing identified three bacterial isolates: Acinetobacter schindleri, Pantoea agglomerans, and Pseudomonas lurida. The three bacteria have different time to reach stationary phases in order to produce their secondary metabolites.
The Effect of Storage Time on The Quality of Common Carp Sperm (Cyprinus carpio) Strain Punten in NaCL Solution and Coconut Water (Cocos nucifera L.) Extender Triandari, Ade; Isna Hapsari, Ajeng Hanum; Iskandar, Rossdannisa; Warih Marhendra, Agung Pramana; Soewondo, Aris; Sri Rahayu
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.05

Abstract

Cyprinus carpio is a freshwater species with high reproduction and adaptability. These factors make C. carpio being the freshwater fish that majority distributed and has high commercial value in some countries, including Indonesia. The maturation phase of male and female fish gonads does not occur simultaneously. Thus, sperm preservation could be an alternative technique to maintain the breeding process throughout the year. This research aims to determine the effect of storage time on C. carpio sperm quality strain Punten in NaCl solution-coconut water (Cocos nucifera L.). This study used common carp (C. carpio) 8 months of age with 500 g.bw-1. The C. carpio semen sample was divided into four treatment groups (0, 3, 6, and 9-hour storage time). The extender used in this research was composed of coconut water (C. nucifera L.) and NaCl solution with a ratio of 70% : 30%. The samples were stored in a refrigerator at 5oC. Fresh sperm sample was analyzed macroscopically using various parameters, such as pH, volume, color, and consistency. Meanwhile, the post-preserved sperm quality sample was analyzed microscopically (motility, viability, concentration, and abnormality). Furthermore, we also obtained the fertilization rate and hatching rate. The result showed that storage time affects the reduction of C. carpio sperm quality significantly (p<0.05). The highest sperm viability was obtained in NaCl solution-Cocos nucifera with a 3-hour storage time (78.33%±5.49). Our study found that the storage time significantly affected the sperm quality of C. carpio that was given with C. nucifera extender. Adding C. nucifera with 3,6,9 hour storage time also increases the fertilization and hatching rate compared with the group without C. nucifera. It can be shown that the addition of C. nucifera as a natural extender has the essential role of maintaining C. carpio sperm quality after a short storage period.
Diversity and Potential Analysis of Liquid Biopesticide Bacteria for Fusarium sp. Control of Shallot -Basal Rot Nur Aini; Suharjono
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.03

Abstract

Shallot basal rot disease (Allium cepa L.) caused by the fungus Fusarium causes many crop losses thus control efforts are required. Microbial biopesticides are used as an alternative to control pests and plant pathogens that are environmentally friendly and target-specific. This study aims to evaluate the diversity of liquid biopesticide bacteria and their potential to control the Fusarium sp. pathogen of shallots. This study includes shallot sampling, isolation, and purification of liquid biopesticide bacteria, liquid biopesticide antifungal test, double culture antagonistic test of bacterial isolates against Fusarium sp., and identification of potential bacterial isolates based on 16S rDNA sequence similarity. The results showed that two bacterial isolates, B7 and B2, had the highest importance value index of 94.21% and 90.59%, respectively, with a bacterial diversity index of 1.44. Liquid biopesticide microbial biomass has a high inhibition zone in the antifungal test against Fusarium sp. with an average inhibition zone of 6.45 mm. Bacterial isolate B2 has the highest potential inhibition of 71.3% against Fusarium sp. and was identified as Genus Serratia with a similarity value of 96.79%.
Secondary Metabolite Profile in Stem and Root of Keji Plant (Staurogyne elongata [Blume] Kuntze) Safitri, Hana; Malik, Abdul; Mukaromah, Arnia Sari
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.02

Abstract

Keji (Staurogyne elongata [Blume] Kuntze) is an herbal medicinal plant that is often found on the Java to Sumatra islands. The leaves and roots of keji (S. elongata) have been used by the community as diuretic drugs, increasing blood pressure and trusted to treat bladder stones, kidney stones, and joint problems. However, research on secondary metabolites in the stems and roots of keji has never been done. The study aimed to identify the secondary metabolite content and marker compounds in the stems and roots of S. elongata. The research stages were drying and sample grinding, extraction, and metabolite compound analysis using Gas Chromatography-Mass Spectrometry (GC-MS). The results showed nine secondary metabolite compounds found in S. elongata stem, such as phenol, terpenoid, and alcohol. Meanwhile, secondary metabolite compounds of S. elongata root were ten compounds from the coumaran, phenols, and ester group. The 2,2’-methylenebis[6-(1,1-dimethylethyl)-4-methyl-phenol is proposed as a marker compound in S. elongata stem. Meanwhile, no secondary metabolites can be used as marker compounds in S. elongata roots because octadecanoic acid, 2-hydroxy-1-(hydroxymethyl)ethyl ester is the primary metabolite.
Water Quality Evaluation of Central Lombok Awang Bay Using Zooplankton Diversity as a Bioindicator Sagista, Reza; Leksono, Amin Setyo; Retnaningdyah, Catur
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.06

Abstract

The water quality in Awang Bay, West Nusa Tenggara, Indonesia, is considered to be polluted due to human activities. This research aims to evaluate the water quality in Teluk Awang based on the physicochemical properties of water and zooplankton as bioindicators. Sampling was conducted at three beach locations: Awang, Ujung Kelor, and Ekas. The physicochemical water quality parameters measured included water temperature, pH, dissolved oxygen (DO), salinity, and transparency. Additionally, environmental factors were observed based on the naturalness and hemeroby index. The result of the identification and counting of the abundance of each zooplankton can then be used to analyze including taxa richness, total density, the diversity index of Shannon-Wiener (HSW), Margalef diversity index, Simpson diversity index, evenness index (E), and dominance index (C). The resulting research indicated that the physicochemical water qualities have met government quality standards for marine biota needs, except for the level of transparency at Ekas Station 3. Ekas Beach has the highest level of transparency, diversity, taxa richness, and total abundance of zooplankton, indicating the best water quality. Ujung Kelor Beach, which has the highest level of naturalness and the highest DO concentration, has moderate zooplankton diversity, indicating that the water quality is in the medium category. Meanwhile, Awang Beach, which had the highest human activity and the worst water quality, was characterized by low levels of transparency and DO, low diversity, taxa richness, and an abundance of zooplankton. Therefore, diversity, taxa richness, and abundance of zooplankton can be used as a bioindicator for changes in water quality.
Evaluation of Water Quality Based on Macrozoobenthos as a Bioindicator in Ngesong Spring and the Ditch, Batu-East Java Susanto, Muhamad Azmi Dwi; Retnaningdyah, Catur; Leksono, Amin Setyo
Biotropika: Journal of Tropical Biology Vol. 12 No. 1 (2024)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2024.012.01.04

Abstract

Ngesong Spring is a clean spring that is used by society as a spring of clean water and as a tourist attraction. The Ngesong spring has a ditch that flows to the society settlement, where the water is used for agricultural and plantation irrigation. The utilization of the Ngesong Spring as a tourist attraction and its ditch through agriculture, settlements, and society settlements has great potential to cause pollution and degradation of water quality, so it is necessary to evaluate water quality. Therefore, it is necessary to monitor the quality of this water, one of which is the use of the macrozoobenthic community structure as a bioindicator. The research was conducted in four locations, namely, a water spring and the channels that will be used for agriculture, plantations, and settlement activities, in September and October 2022. The variables observed in this study included the physicochemical quality of the water, namely, the water temperature, conductivity, power of hydrogen (pH), dissolved oxygen (DO), biological oxygen demand (BOD), and total suspended solids (TSS), as well as the community structure and macrozoobenthos diversity. The results of this study indicate that 26 macrozoobenthic families belong to 12 orders, for a total of 968 individuals. Water springs had the highest diversity and evenness index values, with H = 2.27 and E = 0.54. The analysis of macrozoobenthic family relationships as an indicator of water quality using the family biotic index (FBI) and average score per taxon (ASPT) showed that the watershed site has very clean water and no organic pollution. At watersheds with good water quality, the families Perlidae, Nemouridae, Limnephilidae, Viviparidae, Chironomidae, and Euphaeidae were found. Moreover, In this study, the locations that have highly organically polluted waters are ditches that have agricultural activities and settlement activities.

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