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Dinia R Dwijayanti,
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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
Arjuna Subject : -
Articles 582 Documents
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The Effect of PVP (Polyvinylpyrrolidone) and Incubation Conditions on the Browning and Growth of Porang (Amorphophallus muelleri Blume) Shoot In Vitro Harijati, Nunung; Yapinsky, Clarissa
Biotropika: Journal of Tropical Biology Vol. 14 No. 2 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2026.014.02.17

Abstract

Porang (Amorphophallus muelleri Blume) is a plant with high economic value whose conventional propagation is limited by the availability of planting material. An alternative to overcome this limitation is in vitro propagation. Unfortunately, in vitro propagation is often hampered by tissue browning caused by oxidation of phenolic compounds. This study aimed to determine the effects of polyvinylpyrrolidone (PVP) addition and incubation conditions on porang browning, shoot induction, and shoot propagation. The medium used was Murashige and Skoog (MS) containing 3 ppm BAP and 0.05 ppm NAA. A completely randomised design of two factors was used in this study. The first factor was PVP concentration of 0, 0.3, and 0.5 g/L, and light and dark conditions were the second factor. The explants were bubils, and there were five replicates. The dependent variables were 1) percentage of browning, 2) time of shoot emergence, and 3) number of shoots per explant. The results showed that incubation in dark conditions with  PVP concentration of 0.3 g/L at the shoot induction stage showed the lowest browning percentage, which was 50%. Meanwhile, incubation under light conditions caused the explants to browning of 100% in all PVP treatments. Incubation under light conditions with a PVP concentration of 0.3 g/L was favourable response in accelerating shoot emergence, which was an average of 14 days after culture and produced the highest number of shoots compared to other treatments, averaging eight shoots per explant. To facilitate the large-scale production of superior porang planting material, this study offers a scientific basis for creating an effective and reproducible tissue culture methodology.
Culturable Airborne Bacterial Diversity in Two Different Research Laboratories: The Role of Temperature and Relative Humidity Inayah, Mazidah Noer; Budiarti, Suci Indah; Alfisah, Rizal Khoirun
Biotropika: Journal of Tropical Biology Vol. 14 No. 2 (2026)
Publisher : Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.biotropika.2026.014.02.18

Abstract

Temperature and relative humidity (%RH) are significant variables that affect the composition of airborne bacterial communities. The presence of airborne bacteria in a research laboratory is crucial to investigate, as it affects the laboratory safety. Therefore, the purpose of this study was to examine the effects of temperature and %RH on the structure of airborne bacterial communities in the laboratory and compare the diversity and abundance of airborne bacteria in two research laboratories with different operational characteristics. The exposure plate procedure was used to collect airborne bacterial samples. The types and numbers of bacterial colonies that grew on the media served as data for the abundance and diversity of airborne bacteria, which were evaluated with the statistical analysis software PAST4. The most dominant types of airborne bacteria in both laboratories were identified using 16S rRNA gene sequences. Temperature and %RH data were processed using the GraphPad Prism. According to the findings of this study, the Microbiology Laboratory (LM) has a higher average temperature and a lower %RH than the Biomolecular Laboratory (LB). The average temperature and %RH in the morning (LM: 27.4 oC/ 77.3% and LB: 26.6 oC/ 81.1%) and afternoon (LM: 27.6 oC /76.1% and LB: 26.9 oC/ 79.2%), were not significantly different. However, there were notable differences in the morning and afternoon temperatures and %RH between LM and LB. LM showed greater airborne bacterial diversity than LB, whereas LB exhibited higher bacterial abundance than LM. Gram-positive bacterial species predominated in the airborne bacterial populations in LM and LB. Brevibacterium and Micrococcus were the most common airborne bacteria found in LM and LB, respectively. This finding is intended to provide guidelines for biosafety management and control, particularly in research facilities

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