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Biotropika
Published by Universitas Brawijaya
ISSN : 23027282     EISSN : 25498703     DOI : 10.21776/ub.biotropika.
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Immunology & microbiology
Biotropika: Journal of Tropical Biology invites research articles, short communication, and reviews describing new findings/phenomena of biological sciences in tropical regions, specifically in the following subjects, but not limited to biotechnology, biodiversity, microbiology, botany, zoology, biosystematics, ecology, and environmental sciences.
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Articles 544 Documents
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Perubahan Siklus Estrus Akibat Induksi Peningkatan Kadar Prostaglandin F2α (PGF2α) Pada Fase Luteal Kambing Peranakan Boer Wardhani, Aries Erlinda Ratna; W.M, Agung Pramana
Biotropika: Journal of Tropical Biology Vol 2, No 1 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

ABSTRACT The objective of this study to understand the effect of PGF2α injection in early diestrus phase and mid- diestrus to the estrous cycle. Treatment in this study are early diestrus control, mid- diestrus control, administration of PGF2α on early diestrus phase and administration of PGF2α in mid diestrus phase (each group n=3.) 1.5 ml Capriglandin® was injected once intramusculary. Then, phase change observed every day start one day since injection. Estrous phase were determined by observation vaginal smear cytology. Data were analyzed by Mann- Whitney method using Microsoft Excel software and SPSS 16.0 for Windows. There’s no significant difference in diestrus and proestrus length on injected group at early diestrus and control early diestrus group, onset estrous of injected group and control group respectively on day 15 and on day 16. The injected group at mid diestrus and control mid diestrus group have a significant difference at onset estrous and diestrus length but there is no significant difference in proestrus length, onset of estrous in the injection group and control group respectively on day 6.7 and on day 12 day (P < 0.05). It can be concluded that there is no response of single injection of PGF2α when injected at early diestrus, but when injected at mid- diestrus causes a shorter diestrus phase than the control respectively 2,3 and 8 days. Keywords : PGF2α, onset of estrous, estrous synchronization, vaginal smears
the growth and productivity Pratiwi, Anak Agung Ayu Putri Indra; Arisoesilaningsih, Endang
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

This study aimed to determine the growth and productivity of several local hydromacrophytes such as Acorus calamus L.,
Variasi Spasial Pertumbuhan dan Produktivitas Padi Merah Akibat Pengairan Berbeda di Sawah Organik Desa Sengguruh, Kecamatan Kepanjen Kabupaten Malang Pratiwi, Tiara Ayu; Arisoesilaningsih, Endang
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
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Abstract

This study aimed to observe spatial variation of the Aek Sibundong red rice growth and productivity in three different areas of an organic rice field under different water logging levels, as well as to determine the interaction among rice growth with weeds and rice field’s abiotic factors on two planting seasons in the Sengguruh Village, Kepanjen District Malang Regency. Observations were conducted with a purposive sampling based on water logging in three areas. Red rice growths were observed in four growth phases: 20, 48, 82, 102 dap (days after planting) includes plant height, biomass, number of tillers and panicles, grain number and biomass. Weeds (taxa richness, % coverage and biomass) were observed using sampling plots of 0.25 m2. Abiotic factors include soil (pH, soil organic matter and soil bulk density) and rice field water (presence or absence of logging, pH, and conductivity). Data were analyzed using SPSS and PAST software. The result showed there were spatial variations of rice growth in three areas. Rice planted close by irrigation channel has taller plant height and more number of tillers and panicles but not significantly different from those planted in another two areas. However, rice planted close by irrigation channel tends to produces vegetative biomass and grain ten times more than those planted far by irrigation channel. Water logging decreased coverage and taxa richness of weeds. Some weeds after manual weeding had no effect on productivity of panicles and grains. High content of soils organic matter occurred at early vegetative phase and decreased at late reproductive phase, so the lower soils bulk density. Keywords: Growth, productivity, red rice, spatial variation
Activity of Dexamethasone Therapy on Pro-Inflammatory Cytokines Profile of Balb/c Mice with Biliary Atresia Khasanah, Qonitatul
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
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Abstract

ABSTRACT Biliary atresia is a neonatal obstructive cholangiopathy that progresses to end-stage liver disease. Dexamethasone is one of synthetic glucocorticoid which has function as an anti-inflammatory agent. Here, we investigated whether dexamethasone could modulate the immune activity in mice strain Balb/c with biliary atresia based on the change of quantity of IFN-γ and TNF-α as a pro-inflammatory molecules. This study consists of 2 stages. The first stage is pre-condition which is made the biliary duct become fibrosis by injecting 20 µl of phosphate buffered saline containing 1.5 x 106 fluorescence-forming units Rhesus Rotavirus (RRV) subcutaneously on first day (24 hours) after the mice was born. The second stage is injection with dexamethasone with dose 0,5 mg/kg BW subcutaneously on the 7th-14th day and 14th-21st day. The clinical effect of dexamethasone is investigated on 14th and 21st day by flow cytometry method. Data were analyzed using Kruskal-Wallis test (p<0,05) and Mann-Whitney test using SPSS 16 for Windows. Rotavirus injection subcutaneously was proven to stimulate the production of proinflammatory cytokines, especially in the third week of termination. The result indicated an increasing number of proinflammatory cytokines such as IFN-γ and TNF-α after RRV injection but after injection of dexamethasone the number of those cytokines is decreased. It can be understood that dexamethasone has a capability to reduce the effect of inflammation regard to the decrease of proinflammatory cytokines. Keywords : Biliary atresia, Dexamethasone, Inflammation, Obstructive, Rhesus Rotavirus
Eksplorasi Ektoparasit Pada Ikan Famili Cyprinidae Di Kolam Rumah Makan Wilayah Malang Raya hanum wardany, Karina; Kurniawan, Nia
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
Publisher : Biotropika: Journal of Tropical Biology

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Abstract

Ectoparasites were common found on fishes. Ectoparasites often infect parts of the fins, scales, operculum and gills in fish. This research aims to determine the types of ectoparasites on fish family Cyprinidae and the influence of environmental conditions on the number of ectoparasites. This research was conducted in three pools restaurant in Malang Raya region, then measured abiotic factors of pool water. Fish as the host of ectoparasites, taken using nets and anesthetized by using 3 phenol chloroform. Fish scales and fins were observed. Ectoparasites that attach to the parts taken and put in 70% alcohol. Ectoparasites observed and identified under a microscope. Measurement data of water quality and quantity ectoparasites were analyzed using SPSS 16 program. Argulus japonicus is a species of ectoparasite was founds in B Batu restaurant and I Dau restaurant. While ectoparasites found in G Malang restaurant was Lernaea cyprinacea. Argulus japonicus and Lernaea cyprinacea were exotic species from Japan. The entry of ectoparasites caused by introduction of Koi fish to Indonesia. The results showed that there were significant differences in the number of ectoparasites I Dau with B Batu and G Malang restaurants respectively. The correlation result between the number of ectoparasites and the physico-chemical factors were showed that the pool water DO levels were positively correlated to the number of ectoparasites.   Key word: Ectoparasites, fish family Cyprinidae, restaurant pond at Malang Area, SPSS 16
Aktivitas Ekstrak Etanol Umbi Uwi Ungu (Dioscorea alata L.) terhadap Sel B220+IgE+ pada Mencit BALB/c Model Alergi Pencernaan Christina, Yuyun Ika
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
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Abstract

Uwi ungu (Dioscorea alata L.) merupakan famili Dioscoreaceae yang mengandung diosgenin yang diketahui bermanfaat sebagai agen imunomodulator. Tujuan dari penelitian ini adalah untuk mengetahui adanya perubahan kuantitas sel B220+IgE+ pada organ lymph node mencit BALB/c model alergi pencernaan pasca pemberian ekstrak etanol umbi uwi ungu dan dosis optimum ekstrak etanol umbi uwi ungu. Pemberian ekstrak etanol umbi uwi ungu dilakukan pada hari ke 1-28. Hari ke-15 dilakukan injeksi ovalbumin (OVA) secara intraperitoneal. Kemudian 3 hari setelah injeksi OVA mencit dibedah. Hari ke-22 dilakukan challenge OVA secara intraperitoneal. Hari ke-23 sampai 28 mencit dilakukan injeksi OVA kembali secara oral. Kemudian dilakukan pembedahan pada hari ke-29. Limfosit diisolasi pada organ lymph node dan dilihat profil sel B220+ dan B220+IgE+ melalui analisis flow cytometry. Data dianalisis menggunakan One-way ANOVA (p<0,05) dan uji Tukey menggunakan SPSS 16  for Windows. Jumlah sel B220+IgE+ menurun signifikan pada dosis 0,167 g/kg BB dibandingkan pada dosis 2,008 g/kg BB dan 10,039 g/kg BB. Variasi dosis ekstrak etanol umbi uwi ungu berbeda secara signifikan. Ekstrak etanol umbi uwi ungu (Dioscorea alata L.) mampu menurunkan jumlah relatif sel B220+IgE+ pada dosis 0,167 g/kg BB. Dosis optimum untuk menurunkan level B220+IgE+ adalah pada dosis rendah yaitu 0,167 g/kg BB.Kata kunci : Alergi pencernaan, B220+IgE+, Dioscorea alata L., immunomodulator
Eksplorasi Jenis-Jenis Amfibi di Kawasan OWA Cangar dan Air Terjun Watu Ondo, Gunung Welirang, TAHURA R.Soerjo Izza, Qothrun; Kurniawan, Nia
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
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Abstract

Penelitian ini bertujuan untuk mengetahui jenis amfibi yang dapat ditemukan di kawasan OWA Cangar dan Air Terjun Watu Ondo, TAHURA R.Soerjo, mengetahui karakter habitatnya, serta mengetahui jenis yang berpotensi sebagai bioindikator. Pengambilan data mulai November 2013 hingga Maret 2014 menggunakan metode  Visual Encounter Survey (VES), total usaha 13 jam, 3 pengamat. Lokasi pengambilan data pada ketinggian 1400-1700 mdpl. Faktor abiotik yang diukur meliputi  pH air, suhu (udara & air), dan kelembapan udara. Ditemukan lima jenis amfibi yang seluruhnya tergabung dalam ordo Anura dari lima famili berbeda, yakni katak pohon emas (Philautus aurifasciatus Schlegel, 1837), katak serasah (Leptobrachium hasseltii Tschudi, 1838), bangkong kerdil (Limnonectes microdiscus Boettger, 1892), kongkang jeram (Huia masonii Boulenger, 1884), dan kodok buduk (Duttaphrynus melanostictus Schneider, 1799). Tidak ditemukan satupun spesies dari ordo Caudata maupun Gymnophiona. P. aurifasciatus kerapkali ditemukan pada tumbuhan Psychotria sp., Dypsis lutescens, dan Ficus sp.. L. microdiscus mempunyai habitat daerah hutan dari dataran rendah hingga tinggi. Habitat H. masonii selalu terkait dengan sungai berbatu yang berarus deras dan air jernih. L. hasseltii dapat ditemukan di daerah tinggi di antara serasah hutan. D. melanostictus mempunyai habitat di dekat hunian manusia atau wilayah terganggu. Spesies yang berpotensi besar sebagai bioindikator adalah P. aurifasciatus, H. masonii sebagai indikator perairan jernih, dan D. melanostictus sebagai indikator wilayah terganggu. Kata kunci : Amfibi, Cangar, ketinggian, VES 
Dexamethasone Activities toward Population of B cells, Gr-1, and TNF-α cytokine in Mice (Musmusculus) Balb/c Biliary Atresia Model Rahmawati, Riza
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
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Abstract

Biliary atresia iscondition caused by Rotavirus (RRV) infection. The aims of this study were to know the immune responses of mice model of biliary atresia treated with corticosteroid.Mice were splitinto 3 treatment groups: control (K), RRV injection (R), and RRV injection in the present of dexamethasone(R+D). In R treatment, the baby mice born in <24 hours were injected with 20 µl of phosphate buffered saline containing 1.5 x 106 fluorescence-forming units Rhesus Rotavirus (RRV). First termination was performed in the day 7 to 14, while second termination was done in the day 14 to 21. The dosage of dexamethasone which is applied in this experiment is 0.5mg/kg body weight.Immunocompetent cells were isolated from spleen,  and cell surface molecules were then analyzed by flowcytometry. The data was tested by SPSS 16.0 for Windows program. The results showed that dexamethasone given as corticosteroid for biliarry atresia theurapy couldsuppress TNF-αproduction as well as Gr-1 proliferation. In the other hand dexamethasonecan promote B220+ cell proliferation inrotavirus infected mice. Key word: Baby mice, biliary atresia, dexamethason, flowcytometry, rotavirus
Histopatologi Hati dan Paru Mencit (Mus musculus) yang Terpapar Formalin dan Benzo(α)pyrene Wahyuningroom, Rike; Soewondo, Aris; Widyarti, Sri
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
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Abstract

ABSTRAK Tujuan dari penelitian ini adalah untuk mengetahui pengaruh pemberian formalin dan benzo(α)pyrene terhadap struktur jaringan hati dan paru mencit (Mus musculus). Mencit jantan berumur dua bulan terpapar formalin dosis 2 mg/kg berat badan dengan perlakuan oral selama 60 hari dan terpapar benzo(α)pyrene dosis 250 mg/kg berat badan dengan perlakuan injeksi intraperitoneal sebanyak empat kali setelah 30 hari dengan selang waktu satu hari. Stuktur histologi hati dan paru mencit dianalisis secara deskriptif. Jumlah hepatosit yang mengalami nekrosis dianalisis dengan menggunakan uji Mann-Whitney pada software SPSS 16.0 for Windows. Hasil dari penelitian ini menunjukkan bahwa pemberian formalin, benzo(α)pyrene dan kombinasi antara formalin dan benzo(α)pyrene meningkatkan jumlah hepatosit yang mengalami nekrosis. Nekrosis terjadi di sekitar area portal triad dan di sekitar area vena sentralis pada hati mencit, sedangkan nekrosis terjadi di sekitar area vena pada paru mencit. Kata kunci: benzo(α)pyrene, formalin, hati, nekrosis, paru
Siklus Estrus Induk Kambing Peranakan Boer F1 Dengan Perlakuan Penyapihan Dini Pada Masa Post Partum Zakaria, Muhammad Rizar; W.M, Agung Pramana; Ciptadi, Gatot
Biotropika: Journal of Tropical Biology Vol 2, No 2 (2014)
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Abstract

Tujuan penelitian ini adalah mengetahui pengaruh penyapihan dini pada masa post partum terhadap siklus estrus induk kambing peranakan Boer F1 (Crossbreed Boer dan PE). Selama post partum, regulasi siklus estrus akan dihambat oleh prolaktin dan oksitosin yang dipengaruhi oleh mekanisme suckling. Penelitian ini menggunakan sampel sebanyak 9 ekor induk kambing dalam 3 kelompok yakni kelompok penyapihan selang waktu 42 hari post partum (PP), 56 hari post partum (PP), dan 91 hari post partum sebagai kontrol. Pengamatan siklus estrus dengan menggunakan vaginal smear. Data dianalisis menggunakan uji Kruskal Wallis dengan pendekatan kualitatif secara deskriptif. Hasil penelitian menunjukkan bahwa, interval siklus estrus kedua pasca sapih pada induk kambing kontrol  saat fase proestrus adalah 2 hari, fase estrus selama 2 hari, fase metestrus selama 2 - 3 hari, dan fase diestrus terjadi selama 14 - 15 hari. Selain itu siklus estrus pertama pasca sapih pada perlakuan penyapihan 42 hari (6 minggu), 56 hari (8 minggu), dan kontrol pada selang 91 hari (13 minggu) post partum adalah berkisar 16,7 ± 2,65 hari, 17,7 ± 1,53 hari , dan 10,3 ± 3,06 hari. Sedangkan siklus estrus kedua pasca sapih secara berturut – turut adalah berkisar 18 ±  1 hari, 19 ± 1,73 hari, dan 20,7 ± 1,58 hari. Hasil uji analisis menunjukkan bahwa tidak ada beda nyata (p>0,05) antar semua kelompok pada siklus estrus pertama pasca sapih. Kesimpulannya, interval siklus estrus kedua pasca sapih pada kelompok induk kambing sapih 42 hari PP adalah 18 hari dan sapih 56 hari PP adalah 19 hari dan kontrol rata – rata berkisar 20,7 hari. Kata kunci : Estrus, Post partum, Vaginal Smear

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