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INDONESIA
Indonesian Journal of Cancer Chemoprevention
Published by Indonesian Society for Cancer Chemoprevention
ISSN : 23558989     EISSN : 20880197     DOI : -
Core Subject : Health, Science,
Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology
Indonesian Journal of Cancer Chemoprevention (IJCC) is an open access, peer-reviewed, triannual journal devoted to publishing articles on Cancer Chemoprevention including Experimental and Clinical Pharmacology, especially concerning Anti-Oxidants, Anti-Aging, Anti-Inflammation, Anti-Angiogenesis, and Anti-Carcinogenesis; Cancer Detection; Stem Cell Biology; Immunology; in vitro and in silico Exploration of Chemopreventive Mechanism; and Natural Products.
Arjuna Subject : -
Articles 334 Documents
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Total Phenolic Content of Ethanol Extract of Artrocarpus camansi Leave and its Effect to SOD (Superoxide Dismutase) Level in Mice Hendri Asrin; Poppy Anjelisa Zaitun Hasibuan; Marianne Marianne
Indonesian Journal of Cancer Chemoprevention Vol 8, No 3 (2017)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev8iss3pp101-109

Abstract

A free radical is one of the triggers of degenerative diseases that become the biggest cause of death. Excessive production of free radicals can be neutralized by antioxidant. Antioxidants can be generated from within the body (intracellular). One of them by the enzyme SOD (superoxide dismutase). However, when the production of free radicals exceeds the ability of intracellular antioxidants to neutralize it, antioxidants from outside (extracellular) is necessary. The ethanol extract of Artocarpus camansi leaves (EEACL) contains phenolic compounds which has very strong antioxidant activity based on in vitro study using the DPPH method, but the in vivo study about the total phenolic content effect of its leaves toward antioxidant activity has not been done. 25 mice were divided into 5 groups consisting of control group, a group was induced by stress and three groups were induced by stress, but given EEACL with each dose of 50 mg/kg bw, 100 mg/kg bw and 150 mg/kg bw. Inducing stress in the form of psychological stress was carried out for 7 days and continued with the EEACL administration for 7 days. The mice were dissected and the livers were isolated, then the liver morphological was examined using Hematoxyllin Eosin (HE) staining method and SOD level was examined with immunohistochemical staining method. The data were analyzed with One Way ANOVA and Duncan test using SPSS program version 19.0. Total phenolic content of EEACL is 235.03 ± 4.306 mg GAE/ g of sample. The average SOD levels in the control group is 94.05 %, stressed group is 55.94 %, stress with EEACL dose 50 mg/kg bw group is 58.40 %, stress with EEACL dose 100 mg/kg bw group is 79.68 %, stress with EEACL dose 150 mg/kg bw group is 80.90 %. Based on statistical result, SOD level increased along with the increase of EEACL dose, but not significantly (p < 0,05). Total phenolic content of EEACL has an influence to SOD levels. SOD level increased along with the increase of EEACL administration dose. The higher dose of EEACL leading to higher levels of SOD in the mouse liver.Keywords : total phenolic content, superoxide dismutase, antioxidant, Artocarpus camansi
Heartwood of Secang (CAESALPINIA SAPPAN L.) Ethanolic Extract Show Selective Cytotoxic Activities on T47D and Widr Cells But Not on Hela Cells Erlina Rivanti; Bani Adlina Shabrina; Ika Nurzijah; Cyndwika Ayu; Adam Hermawan
Indonesian Journal of Cancer Chemoprevention Vol 7, No 2 (2016)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev7iss2pp60-67

Abstract

The present study investigate the selectivity of heartwood of secang ethanolic extract (SEE) on T47D breast cancer cells, WiDr colon cancer cells, and HeLa cervical cancer cells, compared to Vero normal epithelial cells. The cytotoxic effect was evaluated by using MTT assay  with 24-hour treatment to get IC50values. Selectivity was evaluated by using selectivity index (SI). SEE had a potent cytotoxic activity on T47D and WiDr cancer cells (IC50 <100 µg/ml). IC50 value of HeLa cancer cells was observed on moderate cytotoxic (100 <IC50 <1000 µg/ml). SEE demonstrated more selective to T47D and WiDr than Vero cells (SI > 3), while in HeLa cells is not selective (SI < 3). This result indicating its potential of Caesalpinia sappan as a chemopreventive agent in cancer therapy.Keywords: Cancer, selectivity, Secang, T47D, WiDr, HeLa, Vero
Combination of Tangeretin and 5-Fluorouracil Modulates Cell Cycle and Induce Apoptosis on WiDr Cells Luthfia Indriyani; Adam Hermawan; Riris Istighfari Jenie
Indonesian Journal of Cancer Chemoprevention Vol 3, No 1 (2012)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev3iss1pp364-369

Abstract

Co-chemotherapeutics approaches are increasing in cancer treatment in order mainly to suppress the resistence phenomenon of cancer treatment and to enhance the cytotoxic effect of the main chemotherapeutics agent. Tangeretin has been known to have cytotoxic effect to some cancer cells through some pathways in the cells. To explore the potential effect of tangeretin as co-chemotherapeutics agent this research was subjected to study the cytotoxic effect of tangeretin in combination with 5-Fluoro Uracil (5-FU) on WiDr colon cancer cells covering the modulation of cell cycle and apoptosis induction. Cytotoxic effect was examined by using MTT assay while apoptotis induction was determined by annexin-V flowcytometry. Under MTT assay, tangeretin showed weak cytotoxic activity on the cells. However, tangeretin significantly enhanced the cytotoxic effect of 5-FU on the cells. This co-chemotherapeutics effect likely correlated with cell cycle modulation effect, especially in inducing polyploidy phenomenon as expressed in the flowcytometric graph of the DNA content. This combination also increased apoptosis induction. These result suggest that tangeretin is potential to be developed as co-chemotherapeutic agent for 5-Fu on colon cancer and further molecular mechanism need to be explored.Keywords: Tangeretin, 5-Fluorourasil, WiDr, cell cycle, apoptosis.
Banana Peels (Musa paradisiaca L.) Extract as Phytoestrogen on Ovariectomized Mice Mammary Gland Development by Inducing c-Myc Expression Nanda Resa Pratama; Yurista Gilang; Rita Riata; Adam Hermawan; Muthi&#039; Ikawati; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 2, No 1 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss1pp151-158

Abstract

Hormone Replacement Therapy (HRT) is therapy for estrogen deficiency and post menopausal syndromes, but high cost and unwell-secured therapy. One of alternative therapy is the usage of phytoestrogens. The banana peel contains flavones, flavonol, flavanone and polimethoxyflavone which are potential as phytoestrogen. The purpose of this study was to examine the estrogenic effect of banana peel extract (BPE) development of mammary gland of ovariectomized rats. Estrogenic effects was examined based on in vivo and in silico experiment. For in vivo experiment, female Sprague-dawley rats aged 50 days were ovariectomized. At 70 days of age, 12 rats were treated with BPE 500 mg/kgBB and 1000mg/kgBB, 5 rats were treated with estradiol 2μg/day while others served as control were treated with CMC-Na 0.5% and sacrificed 2 weeks later. The base line ovariectomized rats and base line non-ovariectomized rats were sacrificed at 70 days of age. The in silico experiment examined by molecular docking between myricetin and estrogen receptor alpha (ER-α). The result of in vivo experiment showed that 1000 mg/kgBW BPE induced c-Myc expression and enhance ovariectomized rat mammary gland development significantly. Meanwhile, molecular docking showed that there are hydrogen bond interaction between bioactive compound in BPE and Estrogen Receptor (ER)-α but less powerfull than estrogen and ER-α interaction. In summary, BPE can act as an estrogen agonist, resulting in the enhancement of c-Myc expression.Keywords: banana peels extract (BPE), phytoestrogen, mammary gland, ovariectomized rats
Essential Oil of Javanese Turmeric (Curcuma xanthorrhiza, Roxb) Decrease Level of LDL-Cholesterol and Body Weight In Rats Endang Darmawan; Suwijiyo Pramono
Indonesian Journal of Cancer Chemoprevention Vol 7, No 1 (2016)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev7iss1pp6-8

Abstract

The aim of the present study was to investigate the effect of the essential oil of Javanese turmeric (Curcuma xanthorrhiza, Roxb) on level of low density lipoprotein-cholesterol (LDL-C) and body weight in rats. The groups were induced by high fat diet for 30 days to produce hyperlipidemia. The rats were treated with essential oil 25 mg/kgBW and simvastatin 25 kg/kgBW for 30 days. The LDL-C and body weight were measured on day one before treatment, and on day thirthy of the treatment. The results showed that the essential oil decreased the level of LDL-C and body weight in rats.Keywords: Javanese turmeric, Curcuma xanthorrhiza, essential oil, lipoprotein, simvastatin
Analysis of the Histopathology, TNF-α of Microglia Cells Expression, NRG-1/erbB Oligodendrocyte, and Ki67/Apoptosis of Dentate Gyrus Rattus novergicus Brain After Acute Traumatic Brain Injury Wibi Riawan; Putri Fitri Alfiantya; Oktavia Rahayu Adianingsih; Zulkarnaen Zulkarnaen; Alif Fariz Jazmi; Sitti Ayu Hemas N.
Indonesian Journal of Cancer Chemoprevention Vol 6, No 1 (2015)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev6iss1pp20-29

Abstract

Head trauma or traumatic brain injury (TBI) gives most serious impact on the central nervous system. Several experimental models have been established to mimic different pathogenesis characteristics of TBI. The purpose of this study was to determine whether there is evidence of hystopathological lesions in the brain tissue after Marmorou TBI models. This study uses Rattus norvegicus Sprague Dawley strain. Macroscopic and microscopic observations on the brain tissue were done. Macroscopic lesions were observed in the brain. Microscopic observation was performed with Haematoxylin-Eosin (HE) staining and immunohistochemistry on the distribution of microglia cells and pyramidal cells in the cortex. Meanwhile, the distribution of NRG-1/ErbB, proliferation, and apoptosis were observed in the hippocampus. The results of macroscopic observation showed that there were wounds caused by falling loads and vasodilatation. On microscopic observation, the TBI group showed an increase in neutrophils distribution and distribution of activated microglia to produce TNF-α, and decrease in the number of cortical pyramidal cells significantly. The distribution of NRG-1 tended to decrease after exposure of TBI and had no effect on its receptor, erbB. Exposure of TBI appears to lower the activity of neuronal cells proliferation in dentate gyrus (DG) area and significantly increase the number of apoptotic cells. Marmarou model is a physiological model of TBI that spontaneously occurs following a trauma to the head, for example trauma due to an accident. This data can be used as a preliminary data of inflammation and tissue regeneration of disrupted adult brain. Therefore, this research could be used as the basis in the studies of therapeutic agents in the process of neurogenesis of brain cells.Keywords: traumatic brain injury, ERG-1/ErbB, dentate gyrus, Ki67, TNF-a, microglia
MCF-7 Resistant Doxorubicin are Characterized by Lamelapodia, Strong Adhesion on Substrate and P-gp Overexpression Dyaningtyas Dewi Pamungkas Putri; Sarmoko Sarmoko; Rifki Febriansah; Endah Puspitasari; Nur Ismiyati; Aditya Fitriasari
Indonesian Journal of Cancer Chemoprevention Vol 2, No 3 (2011)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev2iss3pp304-309

Abstract

The prognosis of breast cancer patients is closely associated with the response of tumor cells to chemotherapy agent. Doxorubicin is one of the primary chemotherapeutic agents used for the treatment of breast cancer. Resistance to chemotherapy is believed to cause treatment failure in cancer patients. Furthermore, long time exposure to chemotherapeutic agent induces cancer cells resistance. MCF-7 sensitive cells used as chemoresistance model have overexpression P-gp (P-glycoprotein). Chemoresistance was established by treating MCF-7 cells with 0.5 µg/ml doxorubicin-contained medium for a week. 50% inhibiting concentration (IC50) doxorubicin on MCF-7 cells/DOX were determined using MTT assay. Western blot assay and immunocytochemistry assay was performed to determine the expression of P-gp. Morphological of MCF-7 cell/DOX was changing to become larger and have lamellapodia. IC50 value of doxorubicin was 700 nM on MCF-7/DOX and 400 nM on sensitive MCF-7 cells. The MCF-7/DOX sensitivity to doxorubicin was decreased, shown by 1.5 fold higher IC50 of doxorubicin on MCF-7/DOX compared to MCF-7 sensitive cells. Treatment doxorubicin to sensitive MCF-7 cells leads to the increasing P-gp expression. The P-gp level expression has strong correlation with the low sensitivity of MCF-7/DOX to doxorubicin.Keywords: doxorubicin, resistance cells, sensitive MCF-7 cell
Secretion of Indoleamine 2,3-Dioxygenase, an Immunomodulatory Substance, by Adipose-Derived Mesenchymal Stem Cell Dian Ratih Laksmitawati; Caroline Tan Sardjono; Jeanne A. Pawitan; Mohammad Sadikin; Ferry Sandra
Indonesian Journal of Cancer Chemoprevention Vol 1, No 2 (2010)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev1iss2pp92-98

Abstract

Lipoaspirate, a wasted by product from liposuction procedure recently has been shown to contain abundant adipose-derived-mesenchymal stem cells (MSCs). Mesenchymal stem cells (MSCs) have been studied in many research areas to regenerate many cell lineages. In addition, MSCs have immunomodulatory effect. This capability has been utilized in several clinical studies in hematopoetic stem cell and organ transplantation as a strategy to reduce the risk of Graft versus Host Disease (GvHD). It has been reported that the ‘stimulated’ MSC is able to secrete substances to suppress tissue rejection. One of the substances was known to be indoleamine 2,3-dioxygenase (IDO).  A previous study has characterized the secretion of IDO by bone marrow-derived MSCs stimulated by an inflammatory mediator interferon gamma (IFN-γ). IDO has been detected using Western blot analysis and by High Performance Liquid Chromatography (HPLC) assay. The aim of this study was to detect the presence of IDO in AD-MSCs culture with and without INFγ stimulation. Our study showed that AD-MSC stimulated with IFN-γ significantly secreted high level of IDO as detected by Enzyme-Linked Immuno Sorbent Assay (ELISA). Despite its property as a proinflammatory mediator, IFN-γ has shown to be able to induce IDO secretion in MSC culture which suggests the immuno modulatory effect of MSC. This study clearly demonstrates the potential application of adipose-derived MSC in the immunomodulatory strategy for allogenic transplantation. Keyword : mesenchymal stem cell, indoleamine 2,3-dioxygenase, IFN-γ
Anti-metastatic Profiles of Boesenbergia pandurata towards MCF-7/HER2 Cells Hilyatul Fadliyah; Nindya Budiana Putri; Ziana Walidah; Ika Putri Nurhayati; Muthi Ikawati; Edy Meiyanto
Indonesian Journal of Cancer Chemoprevention Vol 9, No 2 (2018)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev9iss2pp68-77

Abstract

The development of breast cancer at an advanced stage is signed with metastatic phenomenon, triggering the high mortality, mainly for Human Epidermal Growth Factor Receptor (HER)2 positive cancers. Boesenbergia pandurata is well known as medicinal plant possessing anticancer potential due to the cytototoxic and antimetastatic characteristic of its active compound. The aim of this study is to observe the inhibitory effect of Boesenbergia pandurata ethanolic extract (BPEE) in combination with doxorubicin on migration of MCF-7/HER2 cells. The BPEE was prepared by 96% ethanol maceration. Under MTT assay, BPEE decreased the cells viability with IC50 value of 23±3.9 μg/mL. Lamellipodia and wound healing assay analysis showed that 5 μg/mL BPPE and its combination with 10 nM doxorubicin inhibited cells migration after 48 hours observation, while gelatin zymography analysis showed that this combination did not affect the expression of Matrix Metalloproteinase (MMP)2 and MMP9, but single treatment of 5 μg/mL BPEE caused lower expression of both MMPs. The combination of 5 μg/mL BPPE and 10 nM doxorubicin inhibited the cells migration but not affect to the cells viability. Thus, BPEE is potential to be developed as an antimetastatic agent. The mechanism underlying the migratory inhibition effect needs to be explored further.Keywords : Boesenbergia pandurata, doxorubicin, MCF-7/HER2, migrationSubmitted:
Potency of Radical Scavenging Activity and Determination of Total Phenolic Content of Five Ethanolic Extract of Rhizome Zingiberaceae Family Muhammad Da&#039;i; Didiek Setiawan; Rosita Melannisa
Indonesian Journal of Cancer Chemoprevention Vol 4, No 1 (2013)
Publisher : Indonesian Society for Cancer Chemoprevention

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14499/indonesianjcanchemoprev4iss1pp457-462

Abstract

Several studies show that some plants and fruits could protect human body from free radical danger exposure. Zingiberaceae family has some chemical substances that have antiradical activity, such as phenolic compound and flavonoid. The aim of this study is to determine the correlation of total phenolic compound towards its antiradical activity. Antiradical activity assay was determined by DPPH method (1,1 difenil-2-pikrilhidrazil). The total phenolic compound is determined by spectrofotometry using Folin-Ciocalteu reagent and counted as GAE (Gallic Acid Equivalent). Based on this study, the total phenolic concentration of ethanolic extract of jahe (Zingiber officinale (L.) Rosc), kencur (Kaemferia galanga L.), lengkuas (Languas galanga), lempuyang gajah (Zingiber littorale) and lempuyang pahit (Zingiber americana) is 73.99 mg/g; 23.848 mg/g; 37.841 mg/g; 49.725 mg/g and 40.802 mg/g respectively. The radical scavenging activity (IC50) of jahe (27.38 µg/mL), lengkuas (93,27 µg/mL), lempuyang pahit (105,08 µg/mL), meanwhile lempuyang gajah and kencur show low activity of radical scavenging.Keywords: Zingiberaceae, total phenolic compound, antiradical, DPPH

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