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Current Biochemistry
Published by Institut Pertanian Bogor
ISSN : 23557877     EISSN : 23557931     DOI : -
Core Subject : Science, Education,
Biochemistry, Genetics & Molecular Biology Education
Current Biochemistry (CB) publishes the results of original research that contribute significantly to the understanding of the chemical compound and reaction that occur within living organism. Preference will be accorded to manuscripts that develop new concepts or experimantal approaches, particularly in the advancing areas of biochemistry science. Manuscripts that are primarily theoretical in nature or in the field of bioinformatics must be directed toward explaining important results previously not understood, making important predictions that can be experimentally tested, or developing segnificant advances in theory of general interest to biochemists. Submission of manuscripts in emerging areas in biochemistry, chemical biology, biophysics, proteomics, model studies and structures, cellular and molecular biology, computational biochemistry, biotechnology, and new methods development is encouraged especially if they address basic biochemical mechanisms.
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Articles 111 Documents
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Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Oczhinvia Dwitasari; Djarot Sasongko Hami Seno; Mega Safithri
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment
Identification of Bioactive Compounds and α-Glucosidase Inhibition Activity of Caesalpinia bonduc Seed Extract In vitro Dwitasari, Oczhinvia; Hami Seno, Djarot Sasongko; Safithri, Mega
Current Biochemistry Vol. 5 No. 2 (2018)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

This study is aimed for finding alternative ways to treat diabetes. Many studies have beendone before, but focused on identification of α-glucosidase inhibition mechanism. This study wasconducted by extracting Caesalpinia bonduc seed using two methods maceration by ethanol 96%and the others boil by water. The ethanol extract was then fractioned into three fractions, n-hexane,ethyl acetate, and ethanol. To find the bioactive compounds, the ethanol extract was then tested byseveral phytochemistry tests. The phytochemistry test showed positive results for alkaloid, flavonoid,saponin and triterpenoid. The inhibition mechanism was tested using pNPG. The inhibition testshown that the mechanism was a competitive inhibition, by the Dixon plot. The calculated inhibitionconcentrations for 50% substrate (IC50) for ethyl acetate fraction are 1655.8079 μg/ml for 5 mMsubstrate and 803.9521 μg/ml for 10 mM substrate. From the results of this study, we concluded thatthe extract of Caesalpinia bonduc has inhibition activity toward α-glucosidase enzyme. Keywords: α-glucosidase inhibition, Caesalpinia bonduc seed extract, diabetes treatment
Antibacterial Activity of Leaf Extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. Against Streptococcus pneumoniae Nuke Annisa Nasution; I Made Artika; Dodi Safari
Current Biochemistry Vol. 7 No. 1 (2020)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.7.1.1

Abstract

Antibacterial resistance in Streptococcus pneumoniae has been increasing and is one of ongoing global concern. The need to find new antibacterial agents against Streptococcus pneumoniae is of paramount importance. Medicinal plants are prospective sources of antibacterial agents. The aims of the present study were to determine the activity of leaf extraxt of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. against Streptococcus pneumoniae. Leaves of Anredera cordifolia (Ten.) Steenis were extracted using 96% ethanol, while the leaves of Muntingia calabura L were extracted using 100% methanol. The leaf extracts of the two plants obtained were bioassayed for antibacterial activity against Streptococcus pneumoniae ATCC 49619 and a clinical isolate Streptococcus pneumoniae PU 067. Results showed that leaf extracts of both Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. have antibacterial activity in vitro against Streptococcus pneumoniae ATCC 49619 at crude extract concentrations of 25%, 50%, 75% and 100% (w/v). Both plants extracts showed strongest activity against S. pneumoniae ATCC 49619 at extract concentration of 75%. In addition, the extracts of both plants have inhibitory activity against growth of the clinical isolate Streptococcus pneumoniae PU 067. Both plant extracts showed strongest activity against S. pneumoniae PU 067 at extract concentration of 100%. Therefore, leaf extracts of Anredera cordifolia (Ten.) Steenis and Muntingia calabura L. can potentially be used as a source of antibacterial agent for Streptococcus pneumoniae. Keywords: Antibacterial agent, Anredera cordifolia (Ten.) Steenis, Muntingia calabura L., Streptococcus pneumoniae.
Antibiotic-Resistant-Bacterial Infection of Diabetes Mellitus-Induced Ulcers: A Narrative Review on a Healing Ointment Jaka Aryantara Kuntala; Mutmainnah Agustiawan Umar; Qurrotu Ayni; Dimas Andrianto
Current Biochemistry Vol. 7 No. 2 (2020)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.7.2.4

Abstract

Gangrene is the main reason why diabetes mellitus patient’s leg amputation should be done. Natural compounds which are contained in coffee grounds extract can actually act as antibacterial substances by inhibiting the growth of Methicillin-Resistant Staphylococcus aureus (MRSA) that resistant to several antibiotics. Coffee grounds become waste caused by people are underusing this material and the high production level of coffee grounds in Indonesia. The purpose of this study was to examine the content of antimicrobial compounds from coffee grounds that can heal wounds caused by diabetes mellitus and to create a formulation of coffee ground based ointment for diabetes mellitus wound healing. Methods used in this research are narrative review from literatures, normality test, and T-test. The conclusion of this narrative review, natural compounds such a trigonelline, caffeine, and chlorogenic acid found in coffee grounds are capable of MRSA growth inhibition at concentration 44%. Coffee grounds based ointment with 1% (w/w) of ointment preparation is expected to reduce coffee grounds waste by developing new product as diabetes mellitus wound healer.
Antibacterial Activity of Ethanol Extract of Spirulina platensis Prayoga Pannindrya; Mega Safithri; Kustiariyah Tarman
Current Biochemistry Vol. 7 No. 2 (2020)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.7.1.1

Abstract

Spirulina is a microalgae that has been widely used as various supplements and medicines because of its high nutritional content. The need for new antibacterial sources to solve the problem of antibiotic resistance, makes the potential of Spirulina as an antibacterial agent necessary to be researched. This study aims to analyze the antibacterial activity of ethanol extract of Spirulina platensis obtained from the Indonesian region against Staphylococcus aureus and Escherichia coli. Spirulina samples were macerated in ethanol solvent in a ratio of 1:10 (w / v). The antibacterial test used was the disc diffusion method with clindamycin positive control. Antibacterial test results showed that S. platensis in this study did not have antibacterial activity against E. coli and S. aureus.
Characterization and Bioactivity Test of Pectin from Musa balbisiana Peel Extracted using Various Acid Solvents Husnawati Husnawati; Ika Yuni Astutik; Laksmi Ambarsari
Current Biochemistry Vol. 6 No. 1 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.1.1

Abstract

Banana (Musaceaea) is one of the highest production fruits in Indonesia, which amounted to 7,162,685 tons in 2017. Banana peel waste produced can reach 40% of the total production of fresh bananas. Kepok banana peel contains a lot of nutrients and pectin. Pectin levels in banana peel ranged from 1.92% to 3.25% of dry weight. This study aims to characterize pectin from kepok banana peel (Musa balbisiana) extracted using nitric acid, citric acid, and HCl and screening potential of pectin bioactivity based on LC50 values. The highest pectin yield was obtained from extraction using pH 4 citrate buffer which was 3.68 ± 1.23%. In general, the characteristics of the best pectin from kepok banana peel are pectin extracted with HNO3 pH 4 with methoxyl degree 3.74 ± 0.34%, galacturonate content 87.64 ± 8.36%, and esterification degree 24.24 ± 0.098%. Based on the LC50 value the pectin extraction results can be classified into two, namely commercial pectin and pectin HCl pH 1.5 which is low bioactivity (LC50 > 100 ppm), and pH 4 HNO3 pectin and pH 4 citrate buffer pectin which has high bioactivity (LC50 ≤ 30 ppm). Keywords: Cytotoxicity, galakturonat, kepok banana pectin, methoxyl
Antioxidant Status of Sprague-Dawley Female Rat with Curcuminoids Nanoparticles of Balittro Curcuma Laksmi Ambarsari; Riska Febrianti; Edy Djauhari Purwakusumah
Current Biochemistry Vol. 6 No. 1 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.1.2

Abstract

Curcuma (Curcuma xanthorrhiza) is an Indonesian herbs plant with antioxidant activity. This research aimed to measure the increase of curcuminoids bioavailability through the effectivity of nanoparticle curcuminoids as an antioxidant for carbon tetrachloride (CCl4) induced rats. Nanoparticle curcuminoids were produced by homogenization-ultrasonication method showed results particle size of curcuminoids nanoparticle was 141.85±38.82 nm with polydispersity index was 0.233 and entrapment efficiency was 71%. During the treatment, the rat’s body weight was increased. Clinical symptoms of rats (behavior and feces) were normal. Nanoparticle curcuminoids can decrease malondialdehyde concentrations and increase peroxide, glutathione peroxide, and superoxide dismutase activity. Keywords : antioxidant, curcuminoid, solid lipid nanoparticle, Curcuma.
Bioethanol Production from Tapioca-Waste as Potential Additive Fuel for LCGC (Low-Cost Green Car) Danty Oktiana Prastiwi; Novia Anggita; Yudha Putra Arishandy
Current Biochemistry Vol. 6 No. 1 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.1.6

Abstract

The majority style of people in transportation is 24% contribute to increasing premium demand as energy. Unfortunately, fossil fuel is not renewable and limited quantity. Its means, potential biomass of tapioca waste should be an alternative energy source to solve that problem. Today, the biosystem of bioethanol production needs to be optimized to maximize filtration and minimize the production cost. First, composition and time incubation needs to be optimized. The research found that to fermented 50 g waste on 200 mL water, we need 2 g of yeast, and the time of harvesting optimum is fourth days with 17% ethanol. Without acid hydrolyze, tapioca waste in yeast fermentation serves 2600 ppm glucose on the first day and increases significantly on the second day with 2964.77 ppm glucose. This concentration found by DNS (Dinitrosalicylic acid) method. Yeast is the subject that converts glucose on medium to be ethanol. Secondly, that medium distillate gets pure ethanol. Thirdly, this ethanol mix with premium in some concentration, include 0%, 10%, 20%, 30%, 40%, 50%. This variant would do test emission to understand the advantages of tapioca bioethanol compare to fossil fuel. The result of this research should be support government to enhance LCGC (Low-cost green car) program to achieve lower air pollutants, green energy resolution, and cleaner production in tapioca industry. Key Word: bioethanol, emission, formulation, LCGC, tapioca,
Inhibition of α-glucosidase Activity Seed Extract from Mahogany (Swietenia macrophylla King.) Waras Nurcholis; Rini Muthoharoh; Antonius Padua Ratu
Current Biochemistry Vol. 6 No. 1 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.1.7

Abstract

Diabetes mellitus is a chronic disorder that affects the metabolism of blood glucose inside the body. One of the alternative treatments is by using traditional medicinal plants, which have hypoglycemia effects. A mahogany seed is one of the traditional medicinal plants that has been proven to treat diabetes and has been used for generations. In the aqueous extract, ethanol 96 %, ethyl acetate, and hexane from mahogany seeds (Swietenia macrophylla King.) the inhibition of α-glucosidase activity are checked by measuring p-nitrophenol from p-nitrophenyl-α-D-glucopyranose (pNPG) on 400 nm. The percentage value of the inhibition for α-glucosidase ranged from 6,36-56,77 %, with 96 % ethanol extract that has the highest value and the hexane extract with the lowest value. Phytochemical screening revealed the presence of alkaloids, flavonoids, saponins, and triterpenoids, which could be responsible for the bioactivities shown by the 96 % ethanol extract and aqueous extract of S. macrophylla. Keywords : α-glucosidase activity, diabetes mellitus, mahogany seeds (Swietenia macrophylla King.), phytochemical screening.
Potency Evaluation of Nanoparticles Moringa Leaves Extract as a Bioactive Candidate of Eco-Friendly Antifouling Paint Laksmi Ambarsari; Riksa Nur Wahyuni; Agung Isnanto; Rifany Fairuz Aqilah
Current Biochemistry Vol. 6 No. 2 (2019)
Publisher : IPB University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.29244/cb.6.2.2

Abstract

Biofouling has become a serious problem for the maritime industry. To minimize the impact of biofouling are protected by antifouling coating made from Tributytlin - polishing copolymer paints (TBT - SPC paint) which are heavy metal groups. New alternative to replace the antifouling material is with the flavonoids in Moringa leaf extract. The objective of this research to analyze the potential of Moringa leaf ethanol extract, effectiveness and stability of Moringa leaves ethanol extract as antifouling and its potential to be a bioactive candidate in antifouling coating. Moringa leaves were extracted by maceration method using 96% ethanol and the yield was obtained about 9.32%. Flavonoid compounds were isolated using a liquid-liquid extraction method and obtained a yield of 60.11%. Flavonoid compounds are encapsulated in nanoparticles with chitosan. PSA analysis shows particle size nanoparticle moringa leaf extract of 31.26 nm. Activity of antifouling is determined by inhibition and degradation test using P. aeruginosa and B. subtilis. Moringa leaf nanoparticles and Moringa leaf flavonoid extract caused biofilm inhibition of 43.55% and 10.29% for P. aeruginosa, while for B. subtillis 25.85% and 82.58%. The result of biofilm degradation by Moringa leaf nanoparticles and Moringa leaf flavonoid extract was 6.9% and 2.2% for P. aeruginosa, while B. subtillis was 87.85% and 65.91%. Keyword: antifouling coat, moringa leaves, nanoparticles.

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