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Contact Name
Ahmadi Riyanto
Contact Email
medpub@litbang.deptan.go.id
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ahmadi_puslitbangnak@yahoo.com
Editorial Address
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Location
Kota bogor,
Jawa barat
INDONESIA
Jurnal Ilmu Ternak Veteriner
ISSN : 08537380     EISSN : 2252696X     DOI : -
Core Subject : Health,
Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary
Articles 1,756 Documents
Characteristics of seminal plasma and cryopreservation of anoa (Bubalus sp.) semen obtained by electroejaculation ., Yudi; Yusuf, T.L.; Purwantara, B; Sajuthi, D.; Agil, M
Indonesian Journal of Animal and Veterinary Sciences Vol 16, No 1 (2011)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (191.463 KB) | DOI: 10.14334/jitv.v16i1.633

Abstract

The population of anoa, which is an endemic fauna to Indonesia, was getting decrease caused by the illegal hunting and deforestation. Anoa is included in endangered species by IUCN, and Appendix I by CITES. The experiment aimed to characterize the seminal plasma contents and to cryopreserve the anoa semen for artificial insemination application in captivity. The experiment was carried out in Taman Safari Indonesia (Bogor). Semen was collected from 2 anesthetized males (4-10 years) by electroejaculation. Seminal plasma gained by centrifugation of ejaculate (3000 rpm, 20 minutes), and then was evaluated the biochemical contents. Other ejaculates were evaluated macroscopically and microscopically, and then extended in Tris and Na-citrate media to a total concentration of 100 billion cells mL-1. Extended semen was stored at 4oC, and evaluated the motility and viability every 12 h. Frozen semen was made in Tris medium added with 5% of glycerol. The seminal plasma of anoa contained total lipid, Na, Ca and Mg higher than the buffalo, but its total protein, K and Cl were lower. Electrophoresis of seminal plasma using by SDS-PAGE method showed 10 bands of proteins (17-148 kDa). The motility and viability of chilled-extended semen in Tris and Na-citrate media were not significantly different (P > 0.05) during 72 h of evaluation. Extended semen in both of media may applicable for AI program for 24-48 h. Post thawing motility of frozen semen was still low, 26.00 ± 9.62%. Therefore, it is necessary to improve each stages of semen processing, so the motility will increased and resulted high pregnancy in AI program. Key Words: Anoa, Seminal Plasma, Extended Semen, Frozen Semen, Electroejaclator
Cacao pod based ration supplemented with organic Zn: Growth responce on sheeps Puastuti, Wisri; Yulistiani, Dwi; Mathius, I Wayan; Giyai, Fransiscus; Dihansih, Elis
Indonesian Journal of Animal and Veterinary Sciences Vol 15, No 4 (2010)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (78.482 KB) | DOI: 10.14334/jitv.v15i4.666

Abstract

Cacao pod can be used as feed to replace grass. The aims of study was to evaluate the growth response of sheep fed on cacao pod based rations to replace grass and supplemented with organic Zn. Fresh cacao pod was chopped to the size of 1-2 cm. In untreated cacao pod (without ammoniation), chopped cacao pod was dried then milled, while in ammoniated cacao pod, the chopped cacao pod was mixed with urea, 1.5% (w/w) and kept in anaerobic conditions for 7 days. After 7 days it was dried and milled. Both, without ammoniated and ammoniated cacao pod were used for ration formulation. The rations were formulated in iso protein and iso energy (16% CP and 70% TDN). The study used 25 male lambs Sumatera Composite breed at age 7-8 months old with average body weight 18.8 ± 2.86 kg. Experiments were conducted using a randomized block design with number of animal in each group was 5. Five treatment rations evaluated were: R = grass + concentrate (positive control); K = cacao pod + concentrate; KZ = cacao pod + concentrate + organic Zn; KU = cacao pod ammoniated + concentrate; KUZ = cacao pod ammoniated + concentrate + organic Zn. The treatment rations were offered for 10 weeks. The results showed that the consumption of cacao pod based rations was higher (P < 0.05) than the grass-basal ration (75.19 g/kg BW0,75 vs 70.22 g/kg BW0,75). Suplementation of Zn organic to ammoniated or untreated cacao pod resulted in no significant difference in DM, and CP intake. DM, OM, CP digestibility and N retention were relatively similar among the fifth rations from 53.13 to 63.91%, from 54.69 to 66.49%, from 66.0 to 79.28% and from 9.5 to 13.4 g/h respectively. R ration had similar NH3 levels with KU ration (21.57 mM vs 22.94 mM) but higher than the K, KZ and KUZ ration (16.45 mM, 16.62 mM and 13.61 mM). VFA concentration showed no difference among treatments (from 140.32 to 179.76 mM). Sheep fed ration R had similar ADG with K and KZ (92.19 g/h vs 83.13 g/h; 93.44 g/h) but higher than the ration KU and KZ (73.44 g/h and 61.72 g/h). From this study can be concluded that grass-based rations can be replaced by the cacao pod based rations supplemented with organic Zn and resulted in relatively similar ADG of 92.19 g/h vs 93.44 g/h. Key Words: Cacao Pod, Ammoniation, Organic Zn, Sheep
Morphological characters of Panicum maximum cv Gatton planted from different source of sapling A., Fanindi; E, Sutedi
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (306.541 KB) | DOI: 10.14334/jitv.v19i1.988

Abstract

Morphological characters and agronomic information of Panicum maximum cv Gatton is required in forage crop breeding activities. Therefore a research was conducted to determine the morphology and characteristic of generative phase of Panicum maximum cv Gatton. The study was conducted in greenhouses, using a 40 cm diameter pot. The planting medium was soil from Ciawi (Latosol). Research design was Completely Randomized Design, with 10 replications. Treatments used were 2 types of planting material, 1) derived from the pols (vegetative) and 2 seeds. Plants used were Panicum maximum cv Gatton that have been collected and evaluated at the IRIAP, Ciawi. Variables measured were plant morphology, reproductive traits, seed production per panicle and seed quality. Results showes that morphological characters of cv Gatton derived from seed and pols was not significantly different (p > 0.05), whereas the flowering time and the time of seeds mature after cutting derived from the seeds was faster (p < 0.05) than cultivars derived from pols. While the production of seed derived from the seeds was higher (p < 0.05) when compared to that derived from the pols. Key Words: Morphological Characteristics, Seed, Pols, Panicum maximum
Effect of sun flower oil addition (Helianthus annuus) in diet on nutrient intake, growth performance and characteristics of estrous of pre-mating Garut sheep L, Khotijah; R, Zulihar; MA, Setiadi; KG, Wiryawan; DA, Astuti
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (199.873 KB) | DOI: 10.14334/jitv.v19i1.989

Abstract

Nutritional status of ewes at pre-breeding phase is critical for good reproductive process. Thirty-two ewes Garut sheep (initial body weight 22.5±2.21 kg) were used in a completely randomized design to evaluate the effects of addition of sunflower oil in the diet on nutrient intake, growth performance and characteristics of estrous of pre-mating garut sheep. Ewes were fed grass and supplemented with one of four isonitrogenous high concentrate diets containing four levels of sunflower oil; they were (M0) no addition of sunflower oil; (M1) 2% sunflower oil; (M2) 4% sunflower oil and (M3) 6% sunflower oil addition. Results showed that the addition significantly reduced dry matter intake (p < 0.05), highly significantly reduce crude protein, Ca and P intake (p < 0.01), higly significant increased ether extract intake (p < 0.01) and significantly increased long of estrous (p < 0.05). There was no difference in crude fiber intake, TDN intake, body weight gain, length of estrous onset and response of estrous for ewes fed the four experimental diets. Response on estrous of treatments M0, M1, M2 and M3 were 62.50; 50.00; 75,00 and 62.5% respectively. It is concluded that the addition of sunflower oil up to 6% in the pre-mating ration affects the nutrient intake without interferering the performance, and it tends to improve the charachteristic estrous of Garut ewes. Key Words: Nutrient Intake , Estrous, Garut Sheep, Performance, Sunflower Oil
Formation of germline chimera Gaok chicken used circulation primordial germ cells (circulation PGCs) fresh and thawed T, Kostaman; TL, Yusuf; M, Fahrudin; MA, Setiadi; AR, Setioko
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (214.275 KB) | DOI: 10.14334/jitv.v19i1.990

Abstract

Formation of germline chimeras by transfer of chicken primordial germ cells (PGCs) is one of the effective techniques for preservation and regeneration of genetic resources in chickens. This study attempted to form germline chimeras of Gaok chicken buy purifying circulated PGCs of donor embryo before it is transferred to the recipient (White Leghorn chickens=WL) and studied the ability of recipient embryo on survival in incubators, and hatchability. This study used 200 fertile eggs of Gaok and 90 fertile WL breed all of the eggs was incubated at 380C and 60% humidity in a portable incubator. PGCs-circulation of the blood collected Gaok embryos at stage 14-16 were taken from the dorsal aorta, and then purified by centrifugation method using nycodenz. PGCs-circulation results further purification frozen in liquid nitrogen before being transferred to the recipient embryo. The results showed that for the development of embryos transferred to the fresh circulation of PGCs-circulation as many as 25 cells can survive up to day 14, while one of the transferred of 50 and 100 cells into recipient embryos was hatched (10%). On the contrari recipient embryos that are transferred to the frozen PGCs-circulation the embryos development was shorter, and only survived until day 10th (treatment 25 cells), day 14th (treatment of 50 cells) and day 17th (treatment of 100 cells). It is concluded that the amount of PGCs-circulation embryos transferred to the recipient is one factor that influence the success of the development germline chimeras. Key Words: Gaok Chicken, White Leghorn Chicken, Circulated PGCs, Transfer, Germline Chimera
Carcass profile and chemical characteristic of male Kacang goat (Capra aegragus hircus) I, Mirdhayati; J, Hermanianto; CH, Wijaya; D, Sajuthi
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (209.143 KB) | DOI: 10.14334/jitv.v19i1.991

Abstract

The purpose of this research was to characterize the carcass profile and chemical characteristic of male Kacang goat (local name for Capra aegragus hircus) from two different slaughter ages, namely < 1.5 and > 1.5 years old. Kacang goats generally is slaughtered for consumption at these ages. The body part used for chemical analysis was the carcass leg. Data was analyzed statistically by t test. Results showed that there was no profile differences of the carcass from both group ages obtained from similar rearing system and at common range of body weight for slaughtering. Chemical characteristics measured by the contents of protein, fat, moisture, ash and cholesterol level in goat meat showed no differences between the two slaughter ages. Dominant amino acids found in the goat meat was glutamic acid, aspartic acid, leucyne and lysine. Saturated and unsaturated fatty acid profiles in the goat meat did not show any different between the two slaughter ages, except for eicosatrienoic acid, in which the meat from the younger goat had significantly higher content of eicosatrienoic acid. The meat of male Kacang goat of both groups is a potential source of healthy meat, based on its low fat content, functional amino acid composition, higher desirable fatty acids and fatty acid ratio of omega 6 : omega 3 which corresponds to the values recommended by American Heart Association 2008. Key Words: Male Kacang Goat (Capra aegragus hircus), Carcass, Cholesterol, Amino Acids, Fatty Acids
Genetic polymorphism of the β-lactoglobuline gene in Friesian Holstein cows HS, Nury; A, Anggraeni
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (379.997 KB) | DOI: 10.14334/jitv.v19i1.992

Abstract

Milk protein in dairy cattle consists of casein and whey. Whey in milk of dairy cattle is about 20% with the main component is β-lactoglobulin (7-12%). Polymorphism of the β-lactoglobulin gene affects protein and whey production in milk. Selection at a molecular base to improve whey and protein in milk requires information on genetic diversity of the β-lactoglobulin gene, besides other protein genes. This study was aimed to identify genetic polymorphism of the β-lactoglobulin gene in 88 heads of Friesian Holstein (FH) cows at Cikole Dairy Cattle Breeding and Improvement Station (Cikole DCBIS), Lembang. Genotyping was done using Polymerase Chain Reaction - Restriction Fragment Length Polymorphism (PCR-RFLP) method, and a HaeIII restriction enzyme. Genotype frequency, allele frequency, and heterozygosity value were calculated by Nei method. Hardy-Weinberg equilibrium was calculated by chi-square test. Result showed that the β-lactoglobulin gene in the observed HF cattle was polymorphic, in which there were two alleles (A and B) and three genotypes (AA, AB and BB). Frequencies of the A and B alleles were 0.40 and 0.60 respectively; while those of the AA, AB, and BB genotypes were succesively 0.10; 0.60 and 0.30. Heterozygosity value was obtained for 0.483. The β-lactoglobulin gene was in Hardy - Weinberg equilibrium (χ2cal < χ2table). It is concluded that polymorphic of the β-lactoglobulin gene can be used as an initial information for molecular selection on milk protein composition in FH cows. Key Words: HF Cattle, β-lactoglobuline Gene, Genetic Polymorphism, PCR-RFLP
Biolarvacidal activity of methanol extract of Kipahit leaves (Tithonia diversifolia) against larvae of Chrysomya bezziana fly AH, Wardhana; N, Diana
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (264.467 KB) | DOI: 10.14334/jitv.v19i1.993

Abstract

Myiasis or “belatungan” is the infestation of live human and vertebrate animal tissue with dipterous larvae, Chrysomya bezziana. In general, synthetic insecticides were applied to control the disease. However, it causes negative impact on animal product, so that it is required to find an alternative treatment using herbal medicine. The aim of this study was to access activity of methanol extract of Kipahit leaves (Tithonia diversifolia) against various stages of C. bezziana larvae (L1, L2 and L3). Five treatments were applied with five replications, i.e. control/water (P0), 0,5%, 1%, 2% of the extract for PI, PII and PIII, respectively. Another treatment was 0,05% Asuntol for positive control (PIV). Each treatment was added with1% DMSO and twenty larvae were examined for each replication. Bioassay of L1 and L2 was addressed to investigate effect of intestinal toxicity by mixing the extract with Meat-Blood Mixture (MBM) and Larval Rearing Media (LRM) for L1 and L2, respectively. Bioassay of L3 was to investigate effect of contact toxicity through soaking the larvae into the extract solution for 10 seconds followed by incubating in vermiculite at 36oC. All living larvae after being treated by various concentration of the extract were reared to pupae and allowed to emerge as imago. Number of living larvae and pupae, pupae weight and number of imago were observed. All data were analysed using ANOVA followed by Dunnett test at 95% confidential limit. For L2, larval mortality were counted and probit analysed using POLO-PC software, therefore the lethal concentration (LC50 and LC95) and lethal time (LT50 and LT95) were defined. Results demonstrated that 1% of the extract was the most effective concentration which was able to kill the larvae and decrease the pupae weight. It also caused to fail pupation and imago emergence. The further study might be pursued to investigate in vivo assay of the extract in infested livestock. Key Words: Tithonia diversifolia, Myiasis, Chrysomya bezziana, Biolarvasidal
Antigen detection of rabies virus in brain smear using direct Rapid Immunohistochemistry Test R, Damayanti; I, Rahmadani; Y, Fitria
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (397.552 KB) | DOI: 10.14334/jitv.v19i1.994

Abstract

Rabies is zoonotic disease caused by a fatal, neurotropic virus. Rabies virus is classified into the Genus of Lyssavirus under the yang family of Rhabdoviridae. Rabies affecting hot- blooded animals, as well as human. Dogs, cats, monkeys are the vectors or reservoirs for rabies and the virus was transmitted through the saliva after infected animal’s bites. The aim of this study was to conduct rapid diagnosis to detect rabies viral antigen in brain smear using immunohistochemical (IHC) method namely direct Rapid Immunohistochemical Test (dRIT). A total number of 119 brain samples were achieved from Bukittinggi Veterinary Laboratory, West Sumatra. Standardisation and validation of the method were compared to Fluorescent Antibody Test (FAT) as a golden standard for rabies diagnosis. Results show that dRIT was a very good method, it can be performed within two hours without the need of fluorescent microscope. The samples were tested using FAT and from 119 samples tested, 80 (67.23%) samples were positive for rabies and 39 (32.77%) samples were negative for rabies whereas using dRIT showed that 78 (65.54%) samples were positive for rabies and 41 (34.45%) samples were negative for rabies. The dRIT results were validated by comparing them with FAT results as a golden standard for rabies. The relative sensitivity of dRIT to FAT was 97.5% and the relative specificity to FAT was 100% (with Kappa value of 0.976, stated as excellent). The achievement showed that dRIT is very potential diagnostic tool and is highly recommended to be used widely as a rapid diagnosis tool for rabies. Key Words: Rabies, Antigen Detection, Immunohistochemistry, Direct Rapid Immunohistochemical Test (dRIT)
Efficacy of application of vaccine AI H5N1 clade 2.1.3 on Mojosari ducks challenge against AI H5N1 clade 2.3.2 in laboratory conditions R, Indriani; NLPI, Dharmayanti; R.M.A., Adjid
Indonesian Journal of Animal and Veterinary Sciences Vol 19, No 1 (2014)
Publisher : Indonesian Animal Sciences Society

Show Abstract | Download Original | Original Source | Check in Google Scholar | Full PDF (364.093 KB) | DOI: 10.14334/jitv.v19i1.995

Abstract

Influenza virus HPAI H5N1 clade 2.3.2 outbreaks since September 2012 caused high mortality in ducks. Vaccination is one of strategies recommended by government. However, AI H5N1 clade 2.3.2 vaccine not yet available during this research, while AI H5N1 clade 2.1.3 vaccines available in markets. Therefore it was important to do study on efficay of HPAI H5N1 clade 2.1.3. vaccines on duck at laboratory scale. Three groups of Mojosari duck were used in this study, they were 1 group vaccinated with A Vaccine, 1 group vaccinated with B Vaccine, and 1 group as control (not vaccinated). Vacination groups consisted of 9 DOD and control group was consisted of 6 DOD. Vaccination was conducted when the duck at three weeks old of age using single dose recommended by producer. At three weeks later (ducks at 6 weeks old of age) all Groups of ducks were challenged with virus HPAI H5N1 clade 2.3.2 at dose 106 EID50/ml by drops intranasaly. Result showed that Group 1 (vaccinated with A Vaccine) produced 67% protection (3 out of 9 ducks died), Group 2 (vaccinated with B Vaccine) produced 100% protection (non out of 9 ducks died), and Group 3 (control, not vaccinated) produce 0% protection (all of 9 ducks died). This study give an alternative of choise to use AI H5N1 Clade 2.1.3 vaccine with high protection when AI H5N1 Clade 2.3.2 vaccine not available in markets to controll high mortality in ducks caused by HPAI H5N1 clade 2.3.2 outbreaks. Key Words: Duck, HPAI, AI, Avian Influenza, Vaccine

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