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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

84 85 86 87 88

Formation of germline chimera Gaok chicken used circulation primordial germ cells (circulation PGCs) fresh and thawed Kostaman T; Yusuf TL; Fahrudin M; Setiadi MA; Setioko AR
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 1 (2014): MARCH 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Formation of germline chimeras by transfer of chicken primordial germ cells (PGCs) is one of the effective techniques for preservation and regeneration of genetic resources in chickens. This study attempted to form germline chimeras of Gaok chicken buy purifying circulated PGCs of donor embryo before it is transferred to the recipient (White Leghorn chickens=WL) and studied the ability of recipient embryo on survival in incubators, and hatchability. This study used 200 fertile eggs of Gaok and 90 fertile WL breed all of the eggs was incubated at 380C and 60% humidity in a portable incubator. PGCs-circulation of the blood collected Gaok embryos at stage 14-16 were taken from the dorsal aorta, and then purified by centrifugation method using nycodenz. PGCs-circulation results further purification frozen in liquid nitrogen before being transferred to the recipient embryo. The results showed that for the development of embryos transferred to the fresh circulation of PGCs-circulation as many as 25 cells can survive up to day 14, while one of the transferred of 50 and 100 cells into recipient embryos was hatched (10%). On the contrari recipient embryos that are transferred to the frozen PGCs-circulation the embryos development was shorter, and only survived until day 10th (treatment 25 cells), day 14th (treatment of 50 cells) and day 17th (treatment of 100 cells). It is concluded that the amount of PGCs-circulation embryos transferred to the recipient is one factor that influence the success of the development germline chimeras. Key Words: Gaok Chicken, White Leghorn Chicken, Circulated PGCs, Transfer, Germline Chimera

Antigen-capture enzyme-linked immunosorbent assay using monoclonal antibody for detection of bluetongue virus antigen An Indrawati Sendow
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 4 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

antigen-capture enzyme-linked immunosorbent assay (ELISA) using a specific anti-bluetongue virus group was applied to detect bluetongue viral antigen . The test was specific for bluetongue viruses and did not detect the closely related epizootic haemorrhagic disease of deer viruses (EHDV) and other orbiviruses . It was easy to perform and could be established in laboratories which have simple facilities . The antigen-capture ELISA technique is an alternative method to agar gel immunodiffusion and immuno-dot blotting tests to detect bluetongue antigen in infected tissues, Vero cells, Aedes albopictus cells and BHK-21 cell cultures . Keywords : ELISA, monoclonal antibody, antigen detection, BT

Growth of reproductive organ and its effect on laying performance of medium ype layer due to different levels of dietary protein in growing period Edjeng Suprijatna; Dulatip Natawihardja
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 4 (2005): DECEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The purpose of this experiment was to study the optimal of dietary protein level of medium type layer in growing period associated with the growth of reproductive organ and the effect on laying performance. The experiment used 480 pullets of 10 weeks old grouped into three treatments of three protein levels, 12, 15 and 18. In laying period all of the treatments used the same ration with 15% protein. The results showed that dietary protein level in growing period significantly affected the growth of reproduction organ, growth performance and eggs quality. Effects of treatment in laying period was significantly different on laying performance only in early laying period, and subsequent period was not differently different. The conclusion of this experiment was the level of 12% adequate for minimal growth, level of 15% adequate for optimal growth and level 18% showed better performances during laying period. The results suggest that in growing period the dietary protein level is not below 15%. Key Words: Protein, Reproduction Organ, Laying Performance

Molecular characterization of Indonesia avian influenza virus N.L.P.I. Dharmayanti; R Damayanti; R Indriani; A Wiyono; R.M.A. Adjid
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 2 (2005): JUNE 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Avian influenza outbreaks in poultry have been reported in Java island since August 2003. A total of 14 isolates of avian influenza virus has been isolated from October 2003 to October 2004. The viruses have been identified as HPAI H5N1 subtype. All of them were characterized further at genetic level and also for their pathogenicity. Phylogenetic analysis showed all of the avian influenza virus isolates were closely related to avian influenza virus from China (A/Duck/China/E319-2/03(H5N1). Molecular basis of pathogenicity in HA cleavage site indicated that the isolates of avian influenza virus have multiple basic amino acid (B-X-B-R) indicating that all of the isolates representing virulent avian influenza virus (highly pathogenic avian influenza virus). Key Words: Avian Influenza Virus, Molecular Characterization, Poultry, Indonesia

Effect of feeding system and fermented sago waste on performance of broiler chicken I Putu Kompiang; Supriyati .
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 1 (2001): MARCH 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

An experiment, with a split plot experimental design, was conducted to determine the effect of feeding system (full vs choice feeding) and fermented sago waste (AST 5 vs 0%) on the performance of broiler chickens. Complete feed was formulated tocontain 21% crude protein, 2900 kcal ME/kg, without or with 5% AST. Feed for choice feeding was formulated to contain 2900 kcal ME/kg, without or with 5% AST with crude protein 23 or 17%. Four hundreds DOC broilers were used for each treatment divided into 4 replicates (100 birds/replicate) on litter system. Feed and water were given ad lib. during the 4 weeks trial. Data collected included feed consumption (weekly), body weight (bi-weekly), feed conversion ratio (FCR) and protein efficiency ratio (PER) were calculated biweekly. Feeding system has no significant effect on total feed/energy consumption. Choice feeding reduced total protein consumption (P<0.01; 323.5 vs 354.9 gram/head/4weeks), increased body weight gained (P<0.01; 889.5 vs 835 gram/head/4weeks), improved FCR (P<0.05; 1.90 vs 2.03), and PER (P<0.001; 0.37 vs 0.43). Fermented sago waste (AST) had no significant effect on total feed/energy consumption (P<0.05), reduced total protein consumption (P<0.05; 333.6 vs 349.8 gram/head/4weeks), increased body weight gained (P<0.05; 887.5 vs 837 gram/head/4weeks), improved FCR (P<0.05; 1.88 vs 2.06) and PER (P<0.05; 0.38 vs 0.42). It is concluded that broiler chicken had an ability to determine its protein requirement, and AST supplementation significantly improved performance of the birds. Key words: Choice feeding, fermented sago waste, broiler, FCR, PER

Production and purification of streptavidin with higher biotin-binding activity Simson Tarigan; Sumarningsih . .
Jurnal Ilmu Ternak dan Veteriner Vol 19, No 3 (2014): SEPTEMBER 2014
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The objective of this study was to develop practical, efficient method for production, purification and assay of binding activity of streptavidin. Streptomyces avidinii was first propagated on agar plates, the bacterial cells on the agar were scrapped and suspended in a defined synthetic media (4.4 ml/cm2). After 7 days agitation on a rotary shaker (200 rpm/min) at room temprature (≈28°C), the bacterial cells in the culture were pelleted. The culture supernatant was concentrated to 1/62 original volume with 75% saturation ammonium sulphate. After intensive dialysis against ammonium carbonate buffer pH 11, the suspension was loaded into an iminobiotin agarose column chromatography. The adsorbed protein (streptavidin) was eluted with sodium acetate buffer, pH 4, and the eluate was concentrated with an ultrafiltration divice and suspended in PBS. The strepatavidin-binding activity was assayed by a competitive ELISA, a competition between streptavidin in the sample and the HRP-streptavidin conjugate for the biotin (biotinyl IgG) immobilised on wells of a microtitre plate. The detection limit of this assay measured 0.16 µg/ml streptavidin. The method developed in this study produced 160 µg/ml streptavidin in the culture supernatant. After concentration with the ammonium sulphate, the streptavidin concentration increased to 4 mg/ml (69% recovery). At the final step of purification, streptavidin with 10 mg/ml concentration was obtained. The purity of the streptavidin was higher (95%) with a recovary of 19%. The purified streptavidin in this study appeared as a dimer core streotavidin on SDS PAGE and its binding activity was twice as high as that of a commercial one.

The estimation of Lymnaea rubiginosa and other freshwater snails populations in the rice fields and pond in Bogor, West Java S Widjajanti
Jurnal Ilmu Ternak dan Veteriner Vol 3, No 2 (1998)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The effect of feeding supplement and anthelmintic treatments on the establishment of parasites and the performances of lambs was studied in 24 St . Croix x Local Sumatra Crosses infected with Haemonchus contortus larvae (L3) . The study consisted of a 2 x 2 factorial arrangement involving two levels of supplement (1 .6% and 0.5% body weight ) and two levels of larval infection (0 and 3,000 L3 initial dose followed by a 1,000 L3-weekly dose) .The supplement x infection interaction was significant (P< 0.0001) on the egg counts and total serum proteins, but not on the PCV values (P>0.10) . The interaction was resulted from the shift in the magnitude of difference between supplement or between infection level and not by the shift in the rank between treatments . The 1 .6% body weight (BW) group had lower (P<0.0001) mean egg count than the 0.5% BW group (1,588 vs. 7,880) . Consistently, the blood PCV value and total serum proteins of lambs receiving 1 .6% BW supplement were higher (P<0.0001) than the 0.5% BW supplement group (28 .3 vs . 23 .8 and 5 .2 vs . 4.6, respectively) . Infection resulted in decreased feed and nitrogen (N) intake, but had no effect on fecal-N (P>0.0001). N-excretion (fecal-N + Urine-N) was not altered (P>0.10) by Haemonchus infection, but N-retention decreased in infected lambs due to a reduction in N intake . The effect of supplement and Haemonchus infection was significant (P<0.0001) on daily gain . Lambs on the 1 .6% BW supplement group grew faster than on the 0.5% BW supplement group whether they were infected or not . Infected lamb receiving 0.5% BW supplement lost weight during the experiment . It is concluded that an approach that combines the use of anthelmintics and supplement could ameliorate the influence of gastro-intestinal parasites on the performances of lambs, and reduces the intensity of using anthelmintics in controlling the parasites . Key words : Supplement, anthelmintics, Haemonchus contortus, lambs

Water balance of goats in Jeneponto - South Sulawesi under sunlight exposure and water restriction Djoni Prawira Rahardja
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 3 (2007): SEPTEMBER 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Water balance of 5 does of Kacang goat of Jeneponto was studied under the condition of sunlight exposure and water restriction. The study was conducted in dry season with 4 consecutive treatments of 10 d with 4-5 d of adjustment period between two consecutive treatments: (1) indoor and unrestricted water; (2) indoor and restricted water; (3) 10 h outdoor–and unrestricted water; (4) 10 h outdoor – restricted water. The maximum air temperature of outdoor was 39.3OC, and it was 30OC in the indoor environment. In all treatments, the animals were placed in the individual crates. The plasma volume of the goats was higher under sunlight exposure, but it decreased by water restriction, while hematocrite value indicated a reverse responses. Sunlight exposure did not significantly decrease the intake and digestion of organic matter, but water restriction affected significantly and this effect was higher under sunlight exposre. The proportions of water loss through every avenue were maintained relatively constant either under water restriction or sunlight exposure in which the respration rate increased significantly. The findings suggest that sunlight exposure with unrestricted water resulted in a positive water balance without a significant change in organic matter intake and utilization. Water restriction resulted in a negative water balance, reducing organic matter intake and utilization. As the adaptive mechanisms, the goat appeared to be able to withstand in the harsh environment of Jeneponto by expanding plasma volume, increasing body temperature and respiration rate. Key Words: Goat, Water Balance, Sunlight Exposure, Water Restriction, Digestion

Effects of Sallropus androgynus (katuk) leaf extract on growth, fat accumulation and fecal microorganisms in broiler chickens U Santoso; E Handayani; Suharyanto .
Jurnal Ilmu Ternak dan Veteriner Vol 6, No 4 (2001): DECEMBER 2001
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study was conducted to determine the effects of Sauropus androgynus leaf extract on growth, carcass quality and the number of fecal microorganisms in broiler chickens. Forty-eight male Arbor Acres broiler chickens (21-d-old) obtained from a commercial hatchery were used in the present study. Experiment consisted of four treatment groups with four pen replicates of three broilers allotted randomly to each dietary treatment from day 21-42 old. One group was the control with no additional Sauropus androgynus leaf extract (SAE) (P0), and other three groups were given drinking water supplemented with 1.5 g (PI), 3.0 g (P2) or 4.5 g SAE/l water (P3). The diet used was a commercial mix (Crude protein: 19% and Metabolizable Energy 3200 kcal/kg). Feed intake significantly reduced in P2 or P3 as compared with the control (P<0.05). A decrease in feed conversion ratio was observed in treatment groups as compared with Po (P<0.05). Abdominal fat, neck fat and liver fat content were significantly reduced by SAE (P<0.05), while carcass fat content was not significantly different. Number of fecal Escherichia coli in P1 or P3 (P<0,01) and fecal Streptococcus sp and Salmonella sp. were significantly (P<0,01) reduced by SAE supplementation as compared with the control, while fecal Bacillus subtilis in P2 and Lactobacillus sp. in P1 (P<0,01) were significantly higher as compared with other groups. SAE (P2 or P3) also significantly improved meat taste, shank color but lowered meat color (P<0.05) as compared with the control group. It was found that the number of fecal Streptococcus sp was suitable to predict abdominal fat. In conclusion, the inclusion of SAE at 4.5 g/l drinking water resulted in the best performance and carcass quality. Key words: Sauropus androgynus leaf extract, fat accumulation, broilers

A study on the infection rate of coccidia and distribution of coccidiosis of village chickens in South and East Kalimantan Salfina .; A. Hamdan; S. Partoutomo
Jurnal Ilmu Ternak dan Veteriner Vol 1, No 1 (1995)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The infection rate of coccidia and distribution of coccidiosis of village chickens were studied in South Kalimantan (the districts of Hulu Sungai Tengah, Hulu Sungai Selatan, Tanah Laut, and Tapin) and East Kalimantan (the districts of Pasir) . Six hundred and thirty one fecal and intestinal content of clinically infected young and adult birds were sampled, and microscopically and morphologically examined againts the Eimetia species . The result indicated that the average infection rate was 39 .3% where 40 .7% for young and 38 .5% for adult birds with an opg (oocyst per gram) ranging from 40 to 8,200 . Seven species of Eimetia were identified, i .e . E. tenella 23 .6%, E. maxima 13 .6%, E. necattix 11 .3%, E. acervtdina 6 .7%, E. mitis 3 .6%, E. mivati 2.2%, and E. brvnetti 0 .8% . Infection rate of E. tenella was the highest either in South Kalimantan or in East Kalimantan than the other species, while E. brttnetti was the lowest .

Page 86 of 176 | Total Record : 1756

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Ahmadi Riyanto

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Home Page

OAI Link

Editorial Team

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

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Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA