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Jurnal Ilmu Ternak Veteriner

JITV
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Published by Indonesian Center for Animal Research and Development

ISSN : 08537380 EISSN : 2252696X DOI : -

Core Subject : Health,

Veterinary

Aims JITV (Jurnal Ilmu ternak dan Veteriner) or Indonesian Journal of Animal and Veterinary Sciences (IJAVS) aims to publish original research results and reviews on farm tropical animals such as cattle, buffaloes, sheep, goats, pigs, horses, poultry, as well as non domesticated Indonesian endemic animals, such as deers, anoa, babirusa, etc. Scope Indonesian Journal of Animal and Veterinary Sciences . The journal will consider primary research papers from any source if they make an original contribution to the experimental or theoretical understanding and application of theories and methodologies of some aspects of animal science and veterinary

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Hewan dan Zoologi

Articles 1,756 Documents

86 87 88 89 90

Stallion semen cryopreservation using different cryoprotective agents on the skim milk trehalosa extender R.I Arifiantini; I Supriatna
Jurnal Ilmu Ternak dan Veteriner Vol 12, No 2 (2007): JUNE 2007
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Cryoprotective agents (CPAs), protect the sperm during cryopreservation. There are two general classes of CPAs, at first penetrating cryoprotectants, these pass through the sperm membrane and act both intracellular and extracellularly, and the second non-penetrating cryoprotectants, these act only extracellularly. The objective of this study was to evaluate the addition of different CPAs namely glycerol (G), combination of ethylene glycol with glycerol (EG) and dimethilformamide (DMF) using skim milk trehalosa extender. The semen collected from 3 stallions using artificial vagina twice aweeks. The semen was evaluated, centrifugated and diluted in skim milk extender supplemented with 50 mM trehalose and three different CPAs with the concentration of sperm were 200x106 ml-1. Extended semen was then packed into minitub 0.3 ml and equilibrated at 4oC for 2 hours, freeze in the liquid nitrogen vapor for 10 minutes and stored in liquid nitrogen container -196oC. After 24 hours, the semen was thawed at 37oC for 30 second. Results of this experiment indicated that the percentage of motile and viable sperm in skim trehalosa extender using DMF and glycerol better than combination of ethylene glycol and glycerol. Key Words: Cryopreservation, Stallion Sperm, Cryoprotectant

Isolation and identification of lactid acid bacteria originated from king grass (Pennisetum purpureophoides) as candidate of probiotic for livestock Santoso B; Maunatin A; Hariadi BT; Abubakar H
Jurnal Ilmu Ternak dan Veteriner Vol 18, No 2 (2013): JUNE 2013
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A study was conducted to isolate and identify strain of lactic acid bacteria (LAB) isolated from king grass, and to determine their potential as candidate of probiotic for livestock. The LAB was isolated by culturing king grass extract in De Man, Rogosa and Sharpe (MRS) medium. The pure culture LAB was used to identify strain of bacteria using Analytical Profile Index (API) 50 CH kit. The result showed that the strain bacteria was identified as Lactobacillus plantarum. L. plantarum was able to survive in extreme condition at pH 2 and 0.3% bile salt. L. plantarum also survived against pathogenic bacteria i.e. Staphylococcus aureus, Escherechia coli and Salmonella thypi. It is concluded that L. plantarum isolated from king grass could potentially to be used as probiotic for livestock. Key Words: Bile salt, Lactic acid bacteria, Pathogen, Probiotic, pH.

Carcass and physical meat characteristics of thin tail sheep (TTS) based on calpastatin gene (CAST) (Locus intron 5 – exon 6) genotypes variation Muhammad Ihsan Andi Dagong; R Herman; C Sumantri; R.R Noor; M Yamin
Jurnal Ilmu Ternak dan Veteriner Vol 17, No 1 (2012): MARCH 2012
Publisher : Indonesian Center for Animal Research and Development (ICARD)

The quality of sheep carcass is mostly determined by the total lean meat production, meat distribution on the carcass and the quality of meat. Calpastatin gene (CAST) is known to have an association with carcass and meat quality traits. The objective of this research was to identify the association between CAST polymorphisms and carcass characteristics in Thin Tail Sheep (TTS). Thirty three heads of sheep representing three genotypes of CAST (CAST-11, CAST-12 and CAST-22) were identified for carcass and meat characterisation. There was no significant difference between CAST polymorphisms with meat tenderness, pH, water holding capacity and cooking loss, neither with carcass weight and dressing percentage among genotypes. Shoulder proportion of CAST-11 genotype was larger than that of CAST-12 or CAST-22, but the lean meat proportion of CAST-22 genotype in shoulder, rack and loin were higher than those of CAST-11 but not different from CAST-12. The fat percentage of CAST-11 was the highest among the genotypes. CAST-22 genotype has higher lean meat percentage than the CAST-11. Variation in CAST gene could be used as marker assisted selection in sheep for higher lean meat proportion. Key Words: Calpastatin, Polymorphisms, Carcass Characteristics, Meat Quality, Thin Tail Sheep

The use of sodium pyruvate and sodium hydroxide to increase the number of colonies and coloured antigen yield of Mycoplasma gallisepticum Soeripto .
Jurnal Ilmu Ternak dan Veteriner Vol 2, No 3 (1997)
Publisher : Indonesian Center for Animal Research and Development (ICARD)

A trial to increase the number of colonies and coloured antigen yield of Mycoplasma gallisepticum in mycoplasma broth medium was conducted by comparing the use of normal medium, medium with sodium pyruvate and medium with sodium hydroxide. The result showed that medium with §odium pyruvate had increased the number of colonies and antigen yield and was highly significant different (P< 0.01) compared to both normal medium and medium with sodium hydroxide. Medium with sodium hydroxide produced the number of colonies and antigen yield lower than medium with sodium pyruvate, but higher and was highly significant different (P< 0.01) compared to the normalmedium. Key words: Sodium pyruvate, sodium hydroxide, Mycoplasma gallisepticum antigen

Morphological diversity and genetic differentiation of PO cattle in smallholder farmers Hartati .; Sumadi .; Subandriyo .; Tety Hartatik
Jurnal Ilmu Ternak dan Veteriner Vol 15, No 1 (2010): MARCH 2010
Publisher : Indonesian Center for Animal Research and Development (ICARD)

PO cattle is one of the local cattle with high genetic diversity. The aim of this research was to study genetic diversity of PO cattle in smallholder farmer based on morfology and molecular markers. This research was conducted at breeding center in East Java and Central Java include Tuban, Lamongan and Blora regency, since June until December 2008. PO bull used were of 18 months until 24 months of age and cow of 24 months until 36 months of age or at first calving as much as 30 head each location. The variables measured were body weight, body length, withers height, hip height, shoulder point width, chest girth, chest depth, canon bone circumference, head length and head width. Data were analyzed using descriptive statistic, average analysis between subpopulations, discriminant and canonical analysis and mahalanobis distance was done by SAS DISCRIM procedure. Whole blood were collected to get the data of polymorphism DNA microsatellite using technology of PCR and electrophoresis using 6 microsatellite loci. Variable measured were number and size of alel, alel frequency, heterozygosity,genetic distance and fixation index (F-stat). Data were analyzed using analysis of Microsate Toolkit, GENEPOPV4, MSA (Microsatellite Analyser) and Mega 4. The result showed that Blora and Tuban subpopulation have genetic diversity which is relative lower compared to that of Lamongan. The genetic distance which was close presented by Tuban and Blora subpopulation while Lamongan and Tuban subpopulation show far genetic distance. The result of canonical analyses showed high correlation on shoulder point width, head width, body length, withers height and hip height variable so this five variable can be used as distinguishing variables among subpopulation. The result of molecular genetics analysis using microsatellite showed that highest frequency of alel presented by HEL9 locus at Tuban population so this loci have the high polymorfism. The genetic differentiation among subpopulation showed by differentiation FST value among six loci indicating the increasing of inbreeding in the three subpopulations. Key words: Morphological Diversity, Genetic Differentiation, PO Cattle

Response of Calliandra calothyrsus to inoculation of mutant strain of rhizobia N.D Purwantari; E Sutedi
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 3 (2005): SEPTEMBER 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Rifampicin mutants of rhizobial strain CB3171rif50 and CB3090rif100 were the most effective nitrogen fixing strain of rhizobia selected under axenic condition. An experiment was conducted to evaluate the symbiotic response of C. Calothyrsus inoculated by CB3171rif50 or CB3090rif100 grown in the field, on latosol soil with pH 5,2. Plants were either (1) inoculated with mutant strain CB3171rif50, (2) inoculated with mutant strain mutant CB3090rif100 (3) uninoculated and without nitrogen addition or (4) uninoculated and with nitrogen fertilizer as a basal fertilizer. Treatments were replicated four times and arranged in Randomized Complete Block Design. Parameters measured were plant height at early stage of the growth, leaf weight, stem weight, nodule number and weight, proportion of nodule formed by inoculant, N and P content of leaf. Results shows that C. calothyrsus was responded to Rhizobium inoculation. The rate of regrowth was faster for the inoculated plant than that of uninoculated and no nitrogen addition. CB3171rif50 produced significantly (P<0.05) the highest leaf fresh and dry weight. At the first harvest (10 month-old of plant) it produced 2106 g/tree that equivalent to 18.72 ton/ha. The lowest was achieved by uninoculated plant which was 556 g/tree that equivalent to 4.94 ton/ha. The production of shoot was increased at the subsequent harvests. CB3171rif50 was out yielded in all harvest, except for the second harvest. The highest total production of leaf fresh weight was obtained by plant inoculated with strain mutan CB3171rif50 which was 50.62 ton ha-1 year-1, followed by CB3090rif100, which was 39.75 ton ha-1 year-1 and the lowest was obtained by uninoculated plant, in a range of 29.64–30.62 ton ha-1 year-1. Nodule recovery shows that nodule samples from uninoculated plant were not resistant to 50 ppm and 100 ppm rifampicin antibiotic that means not producing mutant strain, indicating that those nodules were from the native rhizobial strains. Recovery of nodules showed that proportion of nodules infected by strain mutant CB3171rif50 was 61.3% from nodules tested and nodules infected by CB3090rif100 was 20%. Both mutant strains inoculation have increased the plant production and reinoculation was not necessary till 19 month-old of the plant. Re-inoculation should be considered after this period. Key Words: Calliandra calothyrsus, Rhizobium, Mutant, Inoculation

Effect of micromineral and phenylpropionic acid on performances of coccus and rod-shaped cellulolytic bacteria degrading fibre of forage Amlius Thalib; B Haryanto; S Kompiang; I-W Mathius; A Aini
Jurnal Ilmu Ternak dan Veteriner Vol 5, No 2 (2000): JUNE 2000
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Performances of coccus and rod-shaped cellulolytic bacteria as inoculum for fermentation of fibrous substrate treated with growth or stimulator factors have been conducted by in vitro. The bacteria were firstly separated and purified based on their morphological shape and followed by identification of their gram type. The treatments as follow : control, Cu (1,0 ppm), Zn (6,0 ppm), Se (0,02 ppm), Fe (16 ppm), Co (0,02 ppm), Mn (4,0 ppm), Mo (0,002 ppm), and phenylpropionic acid (PPA) (30 ppm). These factors were added into fermentation media individually (F.P/S) and as mixture (Mix F.P/S). Substrates used were cellulose and rice straw. Measurements were bacterial digestion of drymatter (DMD), bacterial count, volatile fatty acids (VFA) and NH3-N contents. Gram test showed that inoculum cellulolytic cocci and rods are gram-positive and gram-negative consecutively. The results of treatments showed that Cu, Zn, Co, Mo and PPA improve digestibility of cellulose and rice straw substrates by cellulolytic cocci significantly (p<0.05), and beside Mn, these factors increased the total count of cellulolytic cocci in fermentation medium significantly (p<0.05). The growth or stimulator factors increasing digestibility values of the substrates by cellulolytic rods significantly (p<0.05) were Cu, Zn, Se, Fe, Co, Mn and Mo (except Mn for rice straw substrate). Only 4 elements of the growth and stimulator factors increasing the total count of cellulolytic rods during fermentation of cellulose and rice straw substrates significantly (p<0.05) that is Zn, Co, Mn and Mo. Digestibility of cellulose by cocci is higher than by rods (34.65% vs 29.87%), however, the digestibility of rice straw by both bacteria did not show difference. Digestibility of both cellulose and rice straw substrates was improved by cocci and rods combination and improved further when treated with Mix F.P/S. Parameters of fermentation media ecosystem measured (i.e. total count of bacteria, VFA and NH3-N) were generally changed to be better when treated with Mix F.P/S. It is concluded that performances of cocci and rods in digesting fibrous substrate are different, and growth or stimulator factors improved the activities of both group of bacteria significantly. Key words : Cellulolytic cocci, cellulolytic rods, growth and stimulator factors, bacterial digestion

Molecular analysis of Hemagglutinin Gen of Highly Pathogenic Avian Influenza of H5N1 Subtype Isolated from Waterfowls R Susanti; Retno D Soejoedono; I Gusti Ngurah K Mahardika; I Wayan T Wibawan; Maggy T Suhartono
Jurnal Ilmu Ternak dan Veteriner Vol 13, No 3 (2008): SEPTEMBER 2008
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Avian influenza viruses (AIV) subtype H5N1 isolated from waterfowls in West Java pose the known characteristic of highly pathogenic strains, with polybasic amino acid sequence of cleavage site QRERRRKKR and QRESRRKKR. This research aimed to analyze the important domains of hemagglutinin (HA) gene of those isolates. Fragment of HA gene was amplified using RT-PCR method with specifically-designed primer pairs and sequenced using dideoxy termination method with ABI automatic sequencer (Applied Biosystems). Multiple alignment of nucleotide and deduced amino acid sequences were analyzed using ClustalW of MEGA-3.1 program. Some of biological domains of HA, i.e. antigenic sites, receptor binding pocket, and glycosylation sites of the isolates were polymorphic. The viruses also pose conserved glutamine (Q) and glisin (G) residues at the known receptor binding site, at the position 222 and 224 respectively. This findings clearly show that all AIV subtype H5N1 isolaed from waterfowl preservesthe α-2,3NeuAcGal avian receptor specificity. Key Words: Antigenic Sites, Glycosylation Sites, Receptor Binding Pocket, AIV H5N1, Waterfowls

Polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) Gene at Locus (Pit-1-Hinf1) and its effects on dam body weight and milk production of local sheeps C. Sumantri; D. Herdiana; A. Farajallah; D. Rahmat
Jurnal Ilmu Ternak dan Veteriner Vol 14, No 3 (2009): SEPTEMBER 2009
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Pituitary-Specific Transcription Factor-1 (Pit-1) is a transcription factor with critical role in the transcriptional regulation of multiple genes in the pituitary. The objective of this research was to identify polymorphism of Pituitary-Specific Transcription Factor-1 (Pit-1) gene at Locus (Pit-1-Hinf1) and to investigate any possible associations of Pit-1 genotypes on dam body weight, milk production and milk quality in local sheep at the Jonggol Animal Science Teaching and Research Unit (JASTRU), Fact. Anim. Sci. Bogor. Agric. Univ. A total number of 161 blood samples were collected from 3 local sheep, namely Garut from Wanaraja (55 hd), Garut from Margawati (23 hd) and lactating ewes (83 hd) from JASTRU farm in Bogor. Genomic DNAs were extracted by a standard phenol-chloroform protocol and amplified by a polymerase chain reaction (PCR) techniques, then PCR products were digested with a Hinf1 enzyme restriction. Fragments of Pit-1gene at locus Pit-1-Hinf1 was detected by a silver-staining method. A length of 637 base pairs (bps) of the Pit-1 gene of local sheep was successfully amplified. The Hinf1 restriction enzyme cut the PCR product into three different length of fragments succesively at 345, 137, and 115 bps designated as A allele; whilst B allele had four fragments at 283, 137, 115, and 62 bps respectively. The locus of Pit-1-Hinf1 was polymorphic in local sheep from Jonggol, however it was monomorfic in Garut sheep. The frequencies of A and B alleles were 0,806 and 0,194 respectively. Pit-1 genotypes had no significant effect on dam body weight and milk production. This result is indicating that the use of single locus Pit-1-Hinf1 in Pit-1 gen is less effective to be used as a candidate in selecting dam body weight and milk production in these three local sheep. Key words: Local Sheep, Pit-1 Gene, Polymorphism, Dam, Milk

Utilization of probiotics for controlling clostridial necrotic enteritis in broiler chickens Lily Natalia; Adin Priadi
Jurnal Ilmu Ternak dan Veteriner Vol 10, No 1 (2005): MARCH 2005
Publisher : Indonesian Center for Animal Research and Development (ICARD)

Clostridial necrotic enteritis (CNE) is a common disease among rapidly growing broiler chickens. The purpose of this trial was to study the utilisation of probiotics in controlling experimental CNE in broiler chickens. Chicken normal gut bacterial flora (mucosal starter culture selective/MCS) was used as a competitive exclusion treatment in broiler chicken and its influence to the occurence of clostridial necrotic enteritis were observed. The study comprised of 4 broiler cages treatments of probiotics (2 different dose of MCS, commercial probiotic, 1 cage untreated as control). Probiotics were given orally upon arrival. All groups were given live coccidial vaccine (as predisposing factor for CNE) and challenged with 108 Clostridium perfringens tipe A and C spores on day 10 and 12. The results showed that the probiotics could reduced the incidence and severity of CNE after challenge and improved the performance of chickens treated. Untreated group showed 40% of the mortality due to CNE, and 30% of the chicken showed subclinical necrotic enteritis (SNE). Key Words: Clostridial Necrotic Enteritis, Probiotics, Broiler Chickeni

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Home Page

OAI Link

Editorial Team

Contact

Reviewer

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Contact Name Ahmadi Riyanto

Contact Email medpub@litbang.deptan.go.id

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Location Kota bogor, Jawa barat INDONESIA

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Abstract

Contact Name
Ahmadi Riyanto

Contact Email
medpub@litbang.deptan.go.id

Phone
-

Journal Mail Official
ahmadi_puslitbangnak@yahoo.com

Editorial Address
-

Location
Kota bogor,

Jawa barat

INDONESIA