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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 15 Documents
 1   2 
Search results for , issue "Vol 43, No 3 (2018): September" : 15 Documents clear
Texture, hedonic test and fatty acid profile of goat cheese with L plantarum TW14 and L. rhamnosus TW2 isolates stored at different temperature conditions Setyawardani, T.; Widayaka, K.; Sumarmono, J.; Rahardjo, A. H. D.; Santoso, S. S.; Sulistyowati, M.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.230-237

Abstract

The purpose of this research was to investigate texture, hedonic test and fatty acids profile of goat cheese stored at cold and frozen temperatures for 60 days. Cheese was manufactured from goat milk with addition of probiotics bacteria L.plantarum TW14 and L.rhamnosusTW2 with a ratio of (1:1/v/v). Treatments were arranged in a factorial design, employing two factors, i.e. temperature (cold; frozen) and storage time (0; 15,30,45 and 60 days). Each treatment was repeated three times. Results showed that goat cheese stored at cold temperature has an average hardness-texture of 374.46 ±77.69 gf while that of the cheese stored at frozen temperature has 221.66 ±38.46 gf, which were significantly different (P<0.05). In term of flavor, texture and overall acceptability, there were no significant effects (P>0.05) of storage temperatures and storage time. However, the taste of cheese stored under cold and frozen temperatures was highly significant different (P<0.01). Fatty acids composition of cheese stored for 60 days in a cold storage showed that the highest fatty acid content was SFA which was 64.34% and USFA was 16.76%. During the 60-day storage, the SFA percentage of was relatively stable while the percentage of oleic acid USFA increased. 
Nitrogen degradation of the limestone-urea mixtures in the rumen of goats Harahap, M. A.; Nuswantara, L. K.; Pangestu, E.; Wahyono, F.; Achmadi, J.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.282-288

Abstract

This experiment was aimed to study the degradation kinetics of limestone-urea mixtures in the goats rumen using the nylon bag technique. Samples of limestone were obtained from two limestone mountains, Pamotan Subdistrict of Central Java Province and Wonosari Subdistrict of Yogyakarta Province. The mixtures were created by combining urea at levels 25, 50, 75and 100%; respectively with two limestones on the basis of their Ca contents: L0U100, LP25U75, LP50U50; LP75U25, LW25U75; LW50U50; and LW75U25. The soluble fraction, potentially degradable fraction, the degradation rate of potentially degradable fraction, and effective degradation of respective dry matter (DM) and nitrogen (N) ruminal degradation kinetics were measured in each mixture. The mixture of LP75U25 had lowest effective and degradation rate of potentially degradable fraction (P<0.05) respectively for DM and N compared with those of other mixtures. In conclusion, the limestone-urea mixture of LP75U25 could be suggested as a dietary supplement of ruminal N slow release.
Amino acid and mineral composition of milk from local Ukrainian cows and their crossbreedings with Brown Swiss and Montbeliarde breeds Borshch, A. A.; Borshch, A.V.; Lutsenko, M.M.; Merzlov, S.V.; Kosior, L.T.; Lastovska, I.A.; Pirova, L.V.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.238-246

Abstract

The aim of this study was to analyze the mineral composition of milk, the amino acid composition of milk protein, and the productivity per 305 days of lactation in crossbred cows compared to pure-breed local Ukrainian analogues. The research was conducted at cows of the Ukrainian Black-Spotted (UBS) dairy breed and crossbred cows of the first-generation obtained as a result of crossing with Brown Swiss breed and at cows of the Ukrainian Red-Spotted (URS) dairy breed and crossbred cows of the first-generation obtained by crossing of the URS dairy breed with Montbeliarde breed. In both farms there were two groups of pure-breed and crossbred cows-analogues. The mineral composition of milk and its technological properties in crossbred cows were higher compared to the pure-breed analogues. The fat content of milk crossbred cows exceeded by 0.08-0.16%, protein exceeded by 0.15-0.22%, and the main trace elements include Ca and P – by 2.66-8.83 and 2.12-7.81 mg/100 g of milk, respectively. Milk protein of crossbred cows was marked by a more balanced composition of essential amino acids (EAAs) and their ratio suitable for processing. The amount of EAAs in 100 g of milk protein of crossbred cows was 3.11-6.25% higher compared to local analogues. Crossbred cows dominated pure-breed analogues by the mineral composition of milk. The authors recommend the use of Montbeliarde or Brown Swiss breeds in commercial herds to improve the technological properties of milk.
Identification of KLF3 gene polymorphism in Indonesian Friesian Holstein cattle Agung, P. P.; Laoditta, F.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.195-200

Abstract

The KLF3 gene was a potential genetic marker candidate due to its involvement in many biological processes such as cell proliferation, cell differentiation, and apoptosis. This study was conducted to identify the KLF3 gene polymorphism in the Indonesian FH cattle. A total of 302 individual cattle blood samples from West Java (n=138), Central Java (n=34), and East Java (n=130) provincies were used to obtainDNA samples. The DNA sequencing was performed using 9 samples (each location of sampling represented by 3 samples) for initial identification of the KLF3 gene polymorphismand followed by PCR-RFLP analysis using all DNA samples. There were two SNPs identified in the intron region of the KLF3 gene i.e. SNP g.59607486delC and SNP g.59607554A>G. The SNP g.59607554A>G could be detected using PCR-RFLP method with HpyCH4IV restriction enzyme and resulted three genotypes of the KLF3 gene (AA, AG, and GG) but its heterozygosity value was low in the Indonesian FH cattle. The AA genotype has the highest frequency (0.73), while the AGand GG genotypes frequency were 0.24 and 0.03, respectively. Information about the KLF3 gene polymorphism in this study can be considered for further study to analyse its association with milk and protein yield traits.
Profit analysis of pig farming in rural comunities in Minahasa Regency of North Sulawesi Santa, N. M.; Wantasen, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.289-295

Abstract

The objective of this study was to analyze the factors influencing the profit of pig farms in rural communities in Minahasa Regency, North Sulawesi based on output sales orientation for breeding stock and combination of breeding-fattening models. The research was conducted from July to December 2016, using survey method with multistage random sampling technique. The selected locations were three Districts of Sonder, Tombulu and Kakas based on the largest population of pigs involving randomly one hundred respondents. The variables were included profit, prices of corn, concentrate, bran, medicine, vitamin, animal housing depreciation, the number of piglets, farm experience and output sales orientation. Data were analyzed on the basis of unit output price Cobb-Douglas Profit Function using multiple linear regressions. Research showed that input prices of production factor and number of piglets were influenced profit (P<0.01). Partially, the input prices of corn, concentrate, bran, medicine, vitamins, output sales orientation, and the number of piglets influenced profit(P<0.01). Average profits of farm were IDR 942,823.70/month for breeding models and IDR 3,679,833.85/month for combination of breeding-fattening models, with average numbers of sows were 2.25 heads
Synthesis of collagen from Bali cattle's hide using a combination of acid and alkali on the extracting process Said, M. I.; Burhan, B.; Tensi, T.; Haerati, H.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.247-256

Abstract

The process of pre-extraction is an important stage in the process of collagen synthesis. This stage increases the sensitivity of collagen molecule chains that can affect production yields and collagen properties. The objectives of the study were to synthesize and evaluate halal collagen from Bali cattle’s hide on different -extracting processes. A total of 5 treatments applied in this study, namely: T1 = Ca(OH)2 5% (b/v) ; T2= Ca(OH)2 15% (b/v) ; T3= Ca(OH)2 5% + CH3COOH 5%(b/v) ; T4= Ca(OH)2 15% + CH3COOH 5% (b/v). Each treatment was repeated 4 times. Data were analyzed in a variety of ways using SPSS program. The observed parameters consisted of: 1) yield, 2) viscosity and 3) pH. The results showed that the difference in real pre-extracting process increased the yield and viscosity, but not the pH value. The application of T4 treatment provided the best results compared to other treatments as well as controls to increase the yield and also improve the collagen's physical properties and pH.
Preliminary study of solute carrier family 23 member 3 (SLC23A3) gene as candidate marker for fatty acid traits in Kampung-Broiler crossbred chickens A. Gunawan; S. Y. Basril; K. Listyarini; A. Furqon; W. Bilyaro; J. Jakaria; M. J. Uddin; C. Sumantri
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.201-210

Abstract

SLC23A3 is one of the key genes which control the properties of the of fatty acids content in the meat. The aim of this study was to identify the association of SNP and mRNA expression of SLC23A3 as a candidate marker for fatty acid traits (FAs). Sixty two F2 Kampung × Broilers crossbred chickens population were used in this study. FA composition was measured at 12 weeks of age from thigh meat of crossbred chicken. The PCR-RFLP and qRT-PCR were used for association study and expression analysis of SLC23A3 gene with divergent fatty acid composition. A SNP in coding region c.22385690 A>C of the SLC23A3 gene was associated (P<0.05) with fatty acid composition including stearic acid (C18:0), elaidic acid (C18:1n9t), and linoleic acid (C18:2n6c). The SLC23A3 was detected in liver from high fatty acids (HFA) and low fatty acid (LFA) composition. However, gene expression of SLC23A3 were not differentially expressed between HFA and LFA. These results will explain better understanding of the key important role of the SLC23A3 in fatty acid traits within the liver and will propose SLC23A3 as a potential genomic selection for selection of chickens with fatty acid composition.
Characteristic of different level of fermented concentrate in the rumen metabolism based on in vitro Sari, N. F.; Ridwan, R.; Rohmatussolihat, R.; Fidriyanto, R.; Astuti, W. D.; Widyastuti, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.296-305

Abstract

Digestibility is a description of the ability of feed material that can be utilized by livestock. Higher digestibility of feed material means the total amount of feed content that can be digested in the digestive tract. This study aimed to obtain the proportion of the use of fermentation concentrate in rumen fermentation based on in vitro. The feed used consisted of 7 treatments as follows P1 (20% Pennisetum purpureum: 80% concentrate), P2 (30% P.purpureum : 70% concentrate), P3 (40% P.purpureum : 60% concentrate), P4 (50% P.purpureum : 50% concentrate), P5 (20% concentrate: 80% P.purpureum), P6 (30% concentrate: 70% P.purpureum) and P7 (40% concentrate: 60% P.purpureum). Dry matter digestibility (DMD) and organic matter digestibility (OMD) were done using Theodorou method. Gas production was measured when the samples were incubated at 39oC in the waterbath incubator at hours 2, 4, 6, 8, 10, 12, 24, and 48. Approximately 0.75 g of feed formulation was mixed with 75 mL of rumen fluid buffer. The rumen fluid is taken from 3 fistulated cattles prior to morning feeding. A complete randomized design with three replicates was used. Asymptotic gas production and kinetics were estimated by the Orskov’s model. The results of this study showed that P2 and P3 treatment showed the best digestibility among others treatment with 71.63% ; 71.06% of DMD and 76.42% ; 71.65% of OMD. In conclusion, P2 and P3 treatment can be used as feed for beef cattle. 
Calcium deposition in egg due to substitution of limestone by eggshell flour in feed of laying hens Kismiati, S.; Yuwanta, T.; Zuprizal, Z.; Supadmo, S.; Atmomarsono, U.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.257-264

Abstract

The aim of this research was to evaluate calcium deposition in egg using eggshell flour as a limestone substitute in feed. Two hundreds laying hen of Isa Brown strain of 25 weeks were used in this study. Treatments were diet with 7.5% limestone as control (T0), 2.5% limestone is substituted with eggshell flour(T1), 5% limestone is substituted with eggshell flour (T2) and limestone is substituted with eggshell flour (T3). A completly randomized design were used to allocated the treatments with 5 replications of each. Each experimental unit consists of 10 laying hens. Parameters measured were egg weight, yolk weight, albumen weight, eggshell weight, calcium of egg (yolk, albumen and eggshell), length, weight and Ca of tibia bone. The results showed that substitution of limestone with eggshell flour had significantly effect (P<0.05) on eggshell weight, Ca deposition on yolk, albumen, and Ca of tibia bone but non significantly effect on egg weight, weight and percentage of yolk, weight and percentage of albumen, percentage and Ca of eggshell, length and weight of tibia bone. In conclusion, calcium deposition in yolk was the highest in the use of 7.5% eggshell flour to substitute limestone but obtained the lowest Ca of bone , while calcium deposition in albumen was the highest in the use of 2.5% eggshell flour.
Polymorphism identification of Diacylglycerol Acyltransferase1 gene and its correlation with fat content of Saanen goat Darmoatmodjo, L. M. Y. D.; Widodo, W.; Asmara, W.
Journal of the Indonesian Tropical Animal Agriculture Vol 43, No 3 (2018): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.43.3.211-217

Abstract

Gene markers are often usefull for selection process of cattle on commercial certain phenotypes. Saanen goat has predominance in milk production traits. Milk fat content is one of the commercial traits of milk production. Diacylglycerol Acyltransferase1 (DGAT1) gene was involved in the final process of triglyceride synthesis and associated with milk fat content. Single nucleotide subtitution or insertion-deletion in certain sequence of DGAT1 gene could be gene markers for milk fat content. The objective of this study was to identify differences variation in milk fat content among individual Saanen goats, to identify exon 14-16 polymorphism of DGAT1 gene and to examine its correlation between polymorphism and milk fat content on Saanen goats. The methodologies was collection blood and milk samples, DNA isolation from blood, amplification of DGAT1 genes, sequencing, detection of polymorphism of exon 14-16 DGAT1 gene with Polymerase Chain Reaction-Single Strand Conformation Polymorphism (PCR-SSCP), and examination milk quality on three lactation months using Lactoscan. The average of daily milk production from 70 Saanen goat was 2340±709 mL, 1880±632 mL, 1432±536 mL and average of milk fat content was 3.07±0.52%, 2.53±0.55%, 3.00±0.55% for the first, second and third months of lactation, respectively. The amplicon size of exon 14-16 of DGAT1 gene was ± 350 bp and showed monomorphic pattern from PCR-SSCP. Variation of milk fat content on Saanen goats not caused by polymorphism of exon 14-16 DGAT1 gene.

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