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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 7 Documents
 1 
Search results for , issue "Vol 48, No 1 (2023): March" : 7 Documents clear
Antibody response to Newcastle disease virus recombinant fusion protein in post-vaccinated laying hens J. Jawad; R. W. Astuti; A. Haryanto; N. Wijayanti
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.20-27

Abstract

This research was aimed to analyze antibody response in laying hens post vaccinated by Newcastle Disease Virus (NDV) recombinat Fusion (F) protein which has been succesfully expressed from the F gene of local isolates of NDV from Kulon Progo strain (0663/04/2013), Yogyakarta, Indonesia. The F gene cloned into expression vector plasmid pBT7-N-His. Two types of NDV recombinant vaccine, a concentrated and pure F recombinant protein were used for vaccination. A concentrated recombinant F protein was collected from the centrifugal ultrafiltration process and a pure recombinant F protein was collected from the electroeluted process. Recombinant F protein of NDV was successfully expressed, purified, and visualized by Sodium Dodecyl Sulphate–Polyacrylamide Gel Electrophoresis (SDS-PAGE) with Coomassie Brilliant Blue staining with a molecular weight of 28 kDa. All two types of recombinant F vaccines and a commercial live vaccine as a positive control were injected two times at 14 and 18th weeks old laying hens to analyze the antibody response in serum. In comparison with a commercial live NDV vaccine, indirect Enzyme-linked Immunosorbent Assay (ELISA) revealed that antibody responses were high in both recombinant F protein vaccinated groups. In conclusion, the recombinant F protein has the potential to be developed as a recombinant vaccine candidate to obtain a higher antibody response in laying hens compared to commercially available live NDV vaccines.
Follicle-stimulating hormone receptor gene exploration as possible markers for prolific trait of local goat in Indonesia E. T. Setiatin; D. A. Lestari; D. W. Harjanti; A. Setiaji; E. Kurnianto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.28-36

Abstract

The objective of this study was to discover and identify the effect of SNP of follicle-stimulating hormone receptor (FSHR) gen on prolific traits in Kejobong (KJ) and Etawah grade (EG) doe. A total of 15 blood samples were taken from 11 KJ and 4 EG with various parity and type of birth. The FSHR gene was amplified from the DNA template by Polymerase Chain Reaction (PCR); the PCR products were then sequenced to determine Single Nucleotide Polymorphisms (SNP). Results showed that 3 SNPs were identified, and those SNPs altered amino acid sequence formed 6 haplotypes and divided the doe based on the type of birth. In conclusion, SNPs identified in this study are associated with a prolific trait that can be used as a genetic marker and haplotype 3, 4, and 5 showed the highest prolific on KJ and EG doe.
Feeding selenium-rich fermented palm kernel cake to laying hens produces selenium-rich eggs, increases egg production and quality B. Sundu; H. Hafsah; M. Pamulu; A. Adjis; U. Hatta; E. Kala’langi; G. Sapu; S. Arifuddin; D. Damry; I. G. Putra
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.37-46

Abstract

A study was conducted to determine the effect of selenium (sodium selenite) added to palm kernel cake (PKC) before fermentation on production, selenium content, and quality of eggs. The PKC was added with 0.1% sodium selenite and 1% baking yeast (Fermipan®). The mixture was added with ster-ile distilled water to increase the water content of the substrate. The substrates were fermented at room temperature for 5 days. The fermented products of selenium-rich fermented palm kernel cake (SRFPKC) were dried and fed to the laying hens. The experimental diets used were T-0: without SRF-PKC, T-1: 0.25% SRFPKC, T-2: 0.50% SRFPKC, T-3: 0.75% SRFPKC and T-4: 1.0 % SRFPKC. The diets were given to 180 laying hens aged 22 weeks for 14 weeks. Data on egg production, feed intake and feed conversion ratio were recorded. Feed digestibilities of dry matter and protein were measured based on the method of total fecal collection. Egg qualities and egg weight loss were done using eggs stored for 1 and 30 days. Selenium and cholesterol contents of eggs were also measured. This study used a completely randomized design and was analyzed by analysis of variance. The results showed that the addition of SRFPKC increased egg production, hen day, egg weight, egg selenium, feed digest-ibility, improve FCR (P<0.05) and inhibit the process of decreasing the quality and weight of eggs stored for 30 days (P<0.05). In conclusion, the addition of SRFPKC increased egg production, feed digestibility, and egg selenium, improve FCR and slow down the deterioration of egg quality stored for 30 days.
Dietary supplementation of Spirulina platensis and Saccharomyces cerevisiae on egg quality, physiological condition and ammonia emission of hens at the late laying period H. I. Wahyuni; T. Yudiarti; E. Widiastuti; T. A. Sartono; I. Agusetyaningsih; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.47-57

Abstract

The study was aimed to evaluate the effect of Spirulina platensis and Saccharomyces cerevisiae or its combination on egg quality, physiological condition and ammonia excretion of hens at the late lay-ing period. At 81 weeks old, 144 Lohmann Brown layer chickens were divided into four treatment groups included CON (hens fed basal feed), SP (basal feed + 0.3% S. platensis), SC (basal feed + 0.2% S. cerevisiae), and SPSC (basal feed + 0.3% S. platensis + 0.2% S. cerevisiae). At the end of the study, eggs, intestinal mucosa, digesta, excreta and blood sample were collected. Results showed that albumin index was higher (P<0.05) in SPSC group than in CON, SP and SC. The yolk index and yolk colour were greater (P<0.05) in SP and SPSC groups than in CON and SC. The erythrocyte values were high-er (P<0.05) in SP group compared to CON, SC and SPSC groups. The ileum pH was higher (P<0.05) in SP than in CON, SC and SPSC. Lactic acid bacteria counts were lower (P<0.05) in the caecum of SC and SPSC than in CON and SP groups. The lower (P<0.05) counts of lactose-negative Enterobacte-riaceae were shown in SC and SPSC than in CON. The excreta pH was lower (P<0.05) in SC group compared to CON, SP and SPSC groups. There were better (P<0.05) protein digestibility coefficient and nitrogen retention in SPSC group than others. Faecal ammonia decreased (P<0.05) in SP, SC and SPSC groups. In conclusion, S. platensis improved egg yolk index and colour, increased erythrocyte counts and played an important role in maintaining the balance of bacteria in the intestine resulting in reduced ammonia excretion. Dietary inclusion of S. cerevisiae reduced ammonia excretion of laying hens during the late laying period. 
Association between leptin gene polymorphism and growth traits in Bali cattle T. Kurlyana; T. Hartatik; S. Sumadi
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.1-9

Abstract

Leptin (LEP) gene produces hormone leptin which is secreted by adipose tissue, and plays an im-portant role in energy balance, regulating feed intake, regulating endocrine function, and immune functions. This study aims to identify molecular markers of LEP gene and its association with growth traits based on SNP in Bali cattle. The blood samples were collected from 16 male and 30 female Bali cattle. The growth data were recorded from 2018 to 2020, consist of body weight and body size. Am-plification of leptin gene with polymerase chain reaction (PCR) using pair of primers, Lep-tin_3Forward: 5’- AGCTTGGAAACATGGTGGTC-3’ and Leptin_3Reverse: 5’- CATGATGCTCCCTGGATTCT-3’ with DNA target 898 bp. The SNPs were identified by the direct sequencing technique. Genotypes of the SNPs were identified using sequencing method. Association of LEP genotypes with growth traits was performed using oneway ANOVA. Three DNA polymor-phisms of the LEP gene were found, namely g.2913C/T, g.3260T/C, and g.3549G/A. SNP g.2913C/T was significantly associated (P<0,05) with weaning shoulder height (WSH), weaning body length (WBL), weaning chest circumference (WCC), yearling shoulder height (YSH), yearling body length (YBL), and yearling chest circumference (YCC). Meanwhile, SNPs g.3260T/C and g.3549G/A were not associated with the body weight and body size in Bali cattle. In conclusion, the SNP g.2913C/T can be used as molecular marker for body size in weaning and yearling of Bali Cattle.
Polymorphism of SNP g.8398A>G at prolactin gene and its effect on Indonesian Holstein dairy cow’s milk performance and reproductive traits Y. W. Setyorini; S. Sutopo; E. Kurnianto; S. Sutiyono
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.10-19

Abstract

The objective of this study was to identify the polymorphism of Single Nucleotide Polymorphism (SNP) g.8398A>G at the prolactin gene exon 4 in Indonesian Holstein dairy cows and its effect on milk production, milk quality, and reproductive traits. A total of 140 blood samples were investigated to detect polymorphism by PCR-RFLP method using the RsaI restriction enzyme. Sequencing was per-formed for confirmation of SNP mutation points. The phenotype data collected were milk production, milk quality, and reproductive traits. Data were analyzed using a t-test. The results showed that poly-morphism of SNP g.8398A>G at prolactin gene exon 4 was found in the study populations with AG and GG genotypes. Cows with the GG genotype indicated higher total milk yield and milk protein (P<0.05) than the AG genotype. Polymorphism was not associated with specific gravity, milk fat, milk lactose, total solid non-fat, and reproductive traits. SNP g.8398A>G at prolactin gene exon 4 showed potential as a genetic marker for selecting superior milk production traits.
Growth performance, intestinal morphology, and carcass traits in broiler chicken fed Conocarpus erectus leaf meal M. F. Al-qazzaz; A. M. Humam; H. A. AI- Mashhadani; O. A. Aljumaili; H. N. Ezzat
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 1 (2023): March
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.1.58-73

Abstract

This study evaluated the effects of adding Conocarpus erectus leaf meal to the diet on the perfor-mance, carcass traits, organ weights, and intestinal morphology of broiler chicken. A total of 396 one-day-old Ross 308 broilers were assigned to nine treatments, which included 0, 0.25%, 0.5%, 0.75%, 1%, 1.25%, 1.5%, 1.75%, and 2% C. erectus leaf meal addition to the broiler diet. Feed and bird weights were recorded weekly. On slaughter day, the weights of carcasses and organs were individual-ly reported using a digital scale as well as the intestine samples were pooled for tissue analysis. High levels of C. erectus leaf meal reduced (P<0.01) body weight, body weight gain, and feed conversion ratio. The basal diet and 0.25% C. erectus leaf meal diet reported higher (P<0.01) body weight and body weight gain than did the other treatments. Birds fed 0.25% C. erectus leaf meal supplementation performed similarly to those fed the basal diet. Significantly, with increasing amounts of C. erectus leaf meal in the diets, there was a linear slope decrease in live weight and body weight gain as well as a linear slope rise in the values of feed intake and feed conversion ratio. Carcass trait and relative organ weights were not altered among the dietary treatments. Feeding 1% C. erectus leaf meal diet decreased (P<0.01) relative abdominal fat weight compared to birds fed the control diet. Birds fed dietary C. erectus treatments had higher (P<0.01) villus height, villus width, crypt depth, and lower villus height/crypt depth ratio than did birds fed the control diet. In conclusion, the study indicated that feeding 0.25% C. erectus leaf meal showed no deleterious effects on the growth performance of the broiler. Growth performance and intestinal morphology were linearly reduced when broilers were fed up 2% of C. erectus meal.

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