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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Core Subject : Science, Agriculture,
Agriculture, Biological Sciences & Forestry Earth & Planetary Sciences
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)
Articles 676 Documents
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A SUPPORTING AID FOR BEEF CATTLE INVESTMENT OF FARM HOUSEHOLD IN CENTRAL JAVA Ekowati, T.; Darwanto, D.H.; Nurtini, S.; Suryantini, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 37, No 1 (2012): (March)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.37.1.41-49

Abstract

The research was conducted to analyze some factors influencing production, income, farmhousehold consumption and investment of farm household beef cattle in Central Java. Five districts werepurposively chosen for research location based on the number of beef cattle population, namelyRembang, Blora, Grobogan, Boyolali and Wonogiri. Forty respondents of each district were chosenrandomly using quota sampling. Data were analyzed through Simultaneous Regression and estimated byTwo Stage Least Square (TSLS). The results showed that independent variables were simultaneouslysignificant to dependent variables (production, income, farm household consumption and investment)with the Probability F test 0.0000 and adjusted R2 were 91%; 89%; 96%; 62%, respectively. Thesimulation’s analysis of agribusiness implementation consisted of 1) decreasing 15% of service perconception, 2) increasing of beef cattle breed and number of beef cattle 15% respectively, 3) raising ofprice of rice and number of household member 15% respectively and 4) increasing of income and priceof beef cattle 10% respectively influenced to farm household consumption and investment 0.446% and5.14%, respectively, meanwhile production and income did not change. The research can be concludedthat the independent variables simultaneously significant influenced to production, income, farmhousehold consumption and beef cattle investment. The simulation of changing usage of input factor andprice significantly influenced to farm household consumption and beef cattle investment.
Hydrolyzation of duck meat protein using Bacillus cereus TD5B protease, pepsin, trypsin and their potency as an angiotensin converting enzyme inhibitor Winarti, A.; Rahmawati, F.; Fitriyanto, N. A.; Jamhari, J.; Erwanto, Y.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 3 (2019): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.3.266-276

Abstract

This study was aimed to explore their potency of protein-bioactive of native ducks-meat after enzymatic hydrolysis by Bacillus-cereus TD5B-protease, Pepsin, and Trypsin as an angiotensin-converting enzyme (ACE) inhibitor. The samples: ducks-meats from 10 months age of male Mojosari and Magelang-Duck. The experiments: individually hydrolysis of meat-protein using protease-enzyme (0.1 % w/w) from Bacillus-cereus TD5B, Pepsin, or Trypsin. The observed parameters: protein concentration, protein molecular weight, ACE-inhibitor activity, and IC-value (IC50). Data of protein concentration were statistically analyzed using T-Test, while data of SDS-PAGE and ACE-inhibiting activity were analyzed descriptively. The results showed that soluble protein concentration increased due to the hydrolysis process, from 0.826±0.108 mg/mL to 1.050±0.197 mg/mL (Microbial-protease), 2.122±0.141 mg/mL (pepsin), 1.641±0.071 mg/mL (trypsin) for Mojosari-duck and 0.642±0.038 mg/mL to 1.171±0.534 mg/mL(Microbial-protease), 2.100±0.376 mg/mL(pepsin), 1.725±0.092 mg/mL(trypsin) for Magelang-duck. The SDS-PAGE pattern showed that there was a decrease of molecular weight of duck-meats due to the hydrolysis process, from the range of 196.53-43.88 kDa to the range of 71.35-10.12 kDa. Duck-meat protein hydrolysate had ACE-inhibiting activity 71.7%(Mojosari-Microbial-Protease) IC50 54μg/mL, 57%(Mojosari-Pepsin) IC50 151μg/mL, 75.8%(Mojosari-Trypsin) IC50 51μg/mL and 52.8%(Magelang-Microbial-Protease) IC50 83μg/mL, 78,5%(Magelang-Pepsin) IC50 85μg/mL, 83.9%(Magelang-Trypsin) IC50 22μg/mL. In conclusion, hydrolysate of Magelang duck-meat used Trypsin had better potency as an ACE-inhibitor. 
Back-Matter (June 2017) Kurnianto, Edy
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 2 (2017): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar

Abstract

THE CHROME-TANNED GOAT LEATHER FOR HIGH QUALITY OF BATIK Pancapalaga, W.; Bintoro, V. P.; Pramono, Y. B.; Triatmojo, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 39, No 3 (2014): September
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.39.3.188-193

Abstract

in which the first stage aimed to evaluate sodium silica as batik wax releasing agent on chrome leather.Completely randomized design (CRD) was applied in this study. The concentration of sodium silica wastreated as treatments, in which Tl = 0, T2 = 2 g/L, T3: 4 g/L and T4 = 6 g/L. The second stage of thestudy was aimed to evaluate the type of dye used in batik method dyeing on chrome-tanned goat leather.CRD was used and the treatments were types of dye, i.e. T'1 = acid, T'2= indigosol, T'3 = napthol, T'4 =remazol. In both studies, 9 replicates were applied for each treatment. The use of sodium silicateconcentration of 2 g/L resulted in the percentage of release wax of 91.4% and did not degrade the qualityof chrome leather. The type of acid dyes and naphthol provided the best adhesion and color brightnessand resistance to washing treatment, water, sweat, bend, and rub at 4/5 to 5 on a scale of gray. Inconclusion, chrome tanned goat leather with batik dyeing method was recommended to become one ofthe raw materials of leather goods.
IDENTIFICATION OF MYOSTATIN GENE c.960delG LOCUS POLYMORPHISM IN INDONESIAN LOCAL SHEEP BY USING PCR-SSCP METHOD Sumantri, C.; Jakaria, J.; Yamin, M.; Nuraini, H.; Andreas, E.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 3 (2011): (September)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.3.145-151

Abstract

Myostatin (MSTN) is a protein inhibit muscle growth. This protein is a member of a superfamily ofmolecules called transforming growth factors beta (TGF-b). Deletion in c.960delG (1-bp deletion atposition 960) disrupts the reading frame from amino acid (aa) position 320 to ending in a premature stopcodon in aa position 359 have been found in Norwegian White Sheep. This deletion in the myostatingene is responsible to increase muscle mass, also known as 'double muscling', in sheep. The purpose ofthis study was to identify the polymorphism of myostatin gene in c.960delG locus of local sheep inIndonesia. The 832 DNA sampels from sheep were collected from 13 populations belonging to thePriangan (86), Javanese Thin Tail (389 i.e. Jonggol, Banjar, Ciomas), Javanese Fat Tail (94), West NusaTenggara (136), Rote Island/East Nusa Tenggara (35), Kisar Island/Southwest Maluku (22),Donggala/South East Celebes (45) and Batur cross breed/Wonosobo, Central Java (25). A gene fragmentof MSTN c.960delG length 299 bp was successfully amplified by using the technique of PCR(polymerase chain reaction) and genotyped by SSCP (single strand conformation polymorphism). Theresult showed no polymorphism in this gene. All sheep tested had G/G genotype for c.960delG locus.
Calliandra calothyrsus and Artocarpus heterophyllus as anti-parasite for Bligon Goat Setyono, W.; Kustantinah, K.; Indarto, E.; Dono, N. D.; Zuprizal, Z.; Zulfa, I. H.
Journal of the Indonesian Tropical Animal Agriculture Vol 44, No 4 (2019): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.44.4.400-407

Abstract

This study investigated the nutritional potency and efficacy of tannin-containing forages as anti-parasites sources to support the performance of female Bligon goats. Fifteen female Bligon goats were divided into 3 different groups based on feeding treatments. The first group (KG) served as the control and was fed 100% king grass (dry matter basis). The second group (KGC) was fed 50% king grass and 50% Calliandra calothyrsus leaves (dry matter basis). The third group (KGA) was fed 50% king grass and 50% Artocarpus heterophyllus leaves (dry matter basis). The study showed that supplementation with tannin-containing forages could improve feed consumption and the digestibility of dry matter (DM), organic matter (OM), and crude protein (CP). The groups that received tannin-containing forages had higher body weight gains at 22.85 g/day and 29.52 g/day for KGC and KGA respectively than the control group. The anti-parasites effects were successfully indicated by the declining number of coccidian oocysts in the feces (number/gram feces), which were 3,166; 841; and 450 for KG, KGC, and KGA respectively. Tannin-containing forage supplementation could improve the consumption and digestibility of DM, OM and CP. Supplementation improved the daily body weight gain. The number of coccidian oocysts in animals receiving supplementation was also reduced. The greatest effects on fecal coccidian oocyst reduction were attained with Jackfruit leaves (Artocarpus heterophyllus) supplementation
Effect of white kabesak (Acacia leucophloea Roxb) leaves level in the diet on feed intake and body weight gain of Kacang goat Lawa, E. D. W.; Marjuki, M.; Hartutik, H.; Chuzaemi, S.
Journal of the Indonesian Tropical Animal Agriculture Vol 42, No 4 (2017): December
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.42.4.255-262

Abstract

This study was conducted to analysis the effect of levels of white kabesak (Acacia leucophloea Roxb.) Willd. leaves in the diet on feed intake, digestibility and body weight gain of Kacang goats. The completely randomized block design using 5 treatments and 5 replications was used in this experiment. The treatments were 5 concentrate feeds containing different levels of white kabesak leaves i.e. 0, 10, 20, 30 and 40% in the dry matter (DM) basis (representing T0, T1, T2, T3 and T4 treatments, respectively). The feeds were set up to contain 11.5-12.5% of crude protein (CP). Concentrate feed and native grass was fed at ratio of 60 : 40 was fed to 5 local male goats (age 1-1.5 years old and initial weight of 16.7±5.0 kg). The results showed that DM, organic matter (OM), and CP intake of T0 was not significantly different from those of T1 goats, but it was significantly higher (P<0.05) than those of T2, T3 and T4. The DM, OM, CP, and crude fiber (CF) digestibility as well as body weight gain in T2 goats were significantly higher (P<0.05) and had feed conversion ratio that was significantly better (P<0.05) compared to those of the other feed treatments. In conclusion, the most optimum level of white kabesak (Acacia leucophloea Roxb.) Willd. leaves used in concentrate feed for goat was 20 %.
THE POTENCY OF SUMBA ONGOLE (SO) CATTLE: A STUDY OF GENETIC CHARACTERIZATION AND CARCASS PRODUCTIVITY Agung, P. P.; Anwar, S.; Wulandari, A. S.; Sudiro, A.; Said, S.; Tappa, B.
Journal of the Indonesian Tropical Animal Agriculture Vol 40, No 2 (2015): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.40.2.71-78

Abstract

A study was conducted to assess the genetic characterization of the Sumba Ongole (SO) cattlebased on DNA microsatellites and also to study the potency of SO cattle based on carcass productivity.Blood samples were collected from 28 individual cattle and 12 microsatellite primers as recommendedby FAO were used to identify the genetic characterization of the SO cattle population. Data of carcassproductivity were collected from 506 individual cattle that slaughtered in Karawaci abattoir, Banten,Indonesia. The heterozygosity values of microsatellite loci ranged from 0.143 to 1.000 (mean 0.536).The highest PIC values was 0.814 (locus TGLA122), while the lowest was 0.280 (locus BM1818).Cattle in range of 351-475 kg slaughter weight was most slaughtered in year 2013 and 2014 with carcasspercentage ranged from 52.89% to 53.43%. The highest carcass percentage (56.34%) was obtained fromcattle in range of 626-650 kg slaughter weight while the lowest (51.42%) was obtained from cattle inrange of 250-275 kg slaughter weight. The results of genetic characterization showed that allmicrosatellite locus were highly polymorphic and highly informative for detecting the level of geneticdiversity in the SO cattle population. The results of carcass productivity showed that the SO cattle hasexcellent potential as beef cattle compare with other local breeds cattle in Indonesia.
THE PERFORMANCE OF JAVA AND ONGOLE CROSSBRED BULL UNDER INTENSIVE FEEDING MANAGEMENT Lestari, C.M.S.; Adiwinarti, R.; Arifin, M.; Purnomoadi, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 36, No 2 (2011): (June)
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.36.2.109-113

Abstract

This study was set up to evaluate the performance of Java and Ongole Crossbred (OC) bulls fedconcentrate and rice straw. A total of four Java bulls and four OC bulls were used in this experiment. Thebulls were fed concentrates (50% of the total dry matter feed requirement) and rice straw (ad libitum).The concentrates were consisted of rice bran, beer waste product, copra meal, minerals, with crudeprotein (CP) and total digestible nutrients (TDN) contents of 15.32% and 73.09%, respectively. Theaverage daily gain (ADG), dry matter intake (DMI), protein and energy intake, and feed conversion ratio(FCR) were observed. The results of this study showed that the ADG, DMI, CP and TDN intake, andFCR were not significantly different (p> 0.05). The ADG of Java and OC bulls were 0.58 kg and 0.78kg, respectively. The averages of DMI, CP and TDN intake were 6.59 kg (2.09% of BW), 0.81 kg and4.34 kg for Java bulls whereas for OC bulls were 6.42 kg (2.11% of BW), 0.78 kg, and 4.20 kg,respectively. The FCR of Java bulls was 11.49 and those of OC bulls was 9.21. It can be concluded thatJava and OC bulls raised intensively and fed concentrate and rice straw had the similar performance.
Expression of Recombinant Fusion Protein from Local Isolate of Newcastle Disease Virus and Antibody Response to Recombinant Fusion Protein in Broiler Chickens Post-Vaccination Astuti, R.W.; Wijayanti, N.; Haryanto, A.
Journal of the Indonesian Tropical Animal Agriculture Vol 45, No 2 (2020): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.45.2.78-90

Abstract

This research aimed to express and purify the recombinant Fusion (F) protein of Newcastle Disease Virus (NDV) from a local isolate in Galur, Kulon Progo, Indonesia (0663/04/2013) from recombinant vector plasmid pBT7-N-His F, and to study the antibody response in the broiler sera which were injected with pure recombinant F protein compared with treated broilers that were vaccinated with commercial inactive NDV vaccines and control broilers without vaccination. The results showed that the recombinant F protein of NDV was successfully expressed, purified and visualized by SDS-PAGE with Coomassie Brilliant Blue staining and Westernblotting methods as a specific recombinant F protein with a molecular weight of 28 kDa. The pure recombinant F protein then was injected into broilers to determine the antibody response in broiler serum. Indirect ELISA showed that the production of antibodies was high in F protein vaccinated groups in comparison with other treated and control groups. The recombinant F protein has potential to be developed as a recombinant vaccine candidate after truncating the 6x His-tag part to obtain higher antibody respond if compared with antibody production in broiler serum post vaccinated with some commercially available broiler vaccines.

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