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Journal of the Indonesian Tropical Animal Agriculture
Published by Universitas Diponegoro
ISSN : 20878273     EISSN : 24606278     DOI : -
Journal of the Indonesian Tropical Animal Agriculture (JITAA) is a double blind peer-reviewed publication devoted to disseminate all information contributing to the understanding and development of animal agriculture in the tropics by publication of original research papers
Articles 718 Documents
Genetic diversity of fowl adenovirus serotype 8b isolated from cases of inclusion body hepatitis in commercial broiler chickens N. M. Sohaimi; U. C. Clifford; M. Hair-Bejo
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 2 (2022): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.2.97-106

Abstract

Inclusion body hepatitis (IBH) is a devastating disease of chickens caused by Fowl adenovirus (FAdV) and molecular studies of hexon gene is important for classification of FAdV isolates, epidemiology of IBH infection and development of effective control strategy. The objective of the study was to molecularly characterize FAdV isolates obtained from the field outbreaks of IBH in broiler chicken from 2017 to 2019. Liver and gizzard samples named UPM1701, UPM1801, UPM1802, UPM1901 and UPM1902 were collected and processed for FAdV detection and characterization by PCR. Swollen, necrotic and haemorrhagic liver; erosion of koilin layer of gizzard and enlargement of the proventriculus were observed. The samples were positive for FAdV with 98% to 100% identical to serotype 8b reference strains based on NCBI Blast. Thirty-one nucleotide changes that produced 10 amino acid substitutions were observed in L1 loop region of UPM1701, UPM1901 and UPM1902 isolates. All isolates clustered together with FAdV 8b reference strains and shared common ancestor with UPM1137E2 and UPM04217. Gizzard erosion in FAdV 8b infection is not common and conciding with mutations indicate evolving novel pathogenicity. These mutations could have effect on the epidemiology of IBH and be useful in designing effective prevention and control strategy. 
Farmer’s perceptions on risk and determinants of risk management strategy in integrated cattle and crops farming systems W. Roessali; M. Mukson; S. Nurfadillah; K. Budiraharjo
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 2 (2022): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.2.146-154

Abstract

The research was conducted to analyze farmers’ risk perceptions and factors that influence farm-ers' decisions in implementing risk management strategies to integrated farming of cattle and crops. This study used a survey method by conducting interviews to 150 respondents who were divided into two patterns based on the composition of the farming practice. Pattern I practice cattle-rice-corn-rice business and Pattern II practices cattle-rice-corn-soybean in Grobogan District, Central Java, Indone-sia. Data were collected in January - May 2019 and were analyzed using probit model on the risk man-agement strategies adopted consisting of credit, insurance, partnerships, and off-farm income. The re-sults showed that 59.15 percent of pattern I farmers and 47.06 percent of pattern II farmers perceived the risk of farming due to climate, market, biological and financial in the high category. Farmers' deci-sions in implementing risk management strategies were influenced by farmers' perceptions of climate, market, biological and financial risks.
A cost-effective non-enzymatic isolation method for bovine adipose-derived mesenchymal stem cells: A preliminary study for tropical livestock applications H. Zulfiqar; S. Suyatno; S. Irfan; D. A. Lestari; I. Rahmawati; A. Hafid; F. Saputra; P. S. Sushadi; I. M. Nainggolan; T. Kostaman; H. Herdis; T. P. Priyatno; M. Pangestu
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 2 (2026): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.2.125-134

Abstract

Animal-derived mesenchymal stem cells (MSCs) have potential applications in livestock systems, particularly in regenerative medicine and reproductive biotechnology. In tropical settings, the development of cost-effective and enzyme-free isolation methods is important to support research and applications under limited laboratory resources. This study aimed to preliminarily evaluate bovine adipose-derived mesenchymal stem cells (AD-MSCs) isolated using a non-enzymatic method, focusing on in vitro morphology, marker expression, chromosomal profile, and post-cryopreservation viability. Adipose tissue from Ongole Grade cattle was processed using an explant culture approach. The isolated cells exhibited fibroblast-like morphology, expressed CD44 and CD166, and lacked expression of the hematopoietic marker CD45. Flow cytometry analysis further confirmed CD44 expression at the protein level, with a high proportion of CD44-positive cells. Karyotype analysis showed that most cells retained the normal diploid chromosome number (2n = 60), although minor variations in chromosome morphology were observed. Post-thaw viability remained above 90%, with no observable decline when evaluated using the same cell populations. These findings provide preliminary evidence that non-enzymatic isolation can support the establishment and maintenance of bovine ADMSCs. This approach may serve as a cost-effective and practical alternative for MSC isolation and biobanking in tropical livestock research systems.
Physiological condition, gut morphology and immune responses of broilers supplemented with Moringa oleifera leaf extract, whey protein or their combination I. Agusetyaningsih; B. Marifah; E. Widiastuti; H. I. Wahyuni; T. Yudiarti; T. A. Sartono; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 2 (2023): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.2.126-142

Abstract

To improve growth performance and health, the broiler industry has recently incorporated plant sources and dairy milk byproducts as feed additives. The aim of this study was to investigate the effect of dietary supplementation of Moringa oleifera leaf extract (MOLE) and whey protein concentrate (WPC) or their combination on production performance, physiological status, gut integrity and immune responses of broilers. A total of 364 day old broiler chicks were used and assigned according to a com-pletely randomized design with four dietary treatments, including CONT (basal feed as control), MOLE (basal feed + 1% MOLE), WPC (basal feed + 1% WPC), and MOLE-WPC (basal feed + 0.5% MOLE + 0.5% WPC). Daily weight gain and daily feed intake of broilers were higher in WPC and CONT groups (P<0.05) compared to MOLE group. The low-density lipoprotein (LDL) were lower in CONT and WPC (P<0.05) than in MOLE and MOLE-WPC groups. The serum glutamic oxaloacetic transaminase (SGOT) were lower in MOLE group (P<0.05) than in MOLE-WPC. In comparison to the CONT and WPC groups, the villi height and ratio of villi to crypt depth in the duodenum were more favorable in the MOLE and MOLE-WPC groups. The lactose negative enterobacteria have a lower number (P<0.05) in MOLE group. Histologically, the jejunum, ileum, and spleen were in better condi-tion in the MOLE and MOLE-WPC groups (P<0.05) than in the other groups. In conclusion, supple-menting MOLE, WPC or their combination improved the gut integrity and immune organ, but had no appreciable impact on production performance and physiological status in broilers.
Performance, carcass and meat quality of broilers raised at a high stocking density and supplemented with encapsulated Cosmos caudatus K. leaf extract S. D. Pawesti; E. Widiastuti; R. Murwani; H. I. Wahyuni; T. Yudiarti; T. A. Sartono; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 2 (2022): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.2.107-108

Abstract

The aim of this study was to determine the effect of supplementation of encapsulated Cosmos cau-datus K. leaf extract (ECLE) on performance, carcass and meat quality of broilers at high density pens. Three hundred and seventy broiler chicks were divided into 5 treatments and 5 replications. The treat-ment was conducted on days 15 to 42 in T0 group (10 chicks/m2 + basal feed), T1 (16 chicks/m2 + ba-sal feed), T2 (16 chicks/m2 + basal feed containing ECLE 0.5 g/kg feed), T3 (16 chicks/m2 + ECLE 1.0 g/kg feed), and T4 (16 head/m2 + ECLE 1.5 g/kg feed). ECLE reduced feed consumption, increased body weight gain, improved feed conversion ratio (FCR), increased the European Production Efficien-cy Factor (EPEF) and European Broiler Index (EBI) in T2 and T3 (P<0.05). Carcass weight and per-centage were increased in T3 and T4 (P<0.05). Breast percentage was higher (P<0.05) in T0, T1 and T2; wing and thigh percentages were higher (P<0.05) in T3 and T4; drumstick and giblet percentages were lower (P<0.05) in T3. Drumstick’s pH were higher (P<0.05) in T2, T3 and T4. Thigh fat de-creased (P<0.05) at T4. The redness (a*) in T3 and T4 were significantly better. Overall, at high densi-ty pens, ECLE at 0.5 g/kg feed enhanced broiler performance, carcass percentage, and breast meat quality.
Effect of heat shock program on physical semen characteristics and blood constituents of buck rabbits under hot conditions H. Z. Amer; A. S. Morsy
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 2 (2026): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.2.83-93

Abstract

This study aimed to release the heat shock protein 70 by applying heat shock exposure programs at early ages on physical semen characteristics and blood constituents of Hi-Plus buck rabbits under hot conditions. A total number of 40 Hi-Plus buck rabbits were divided into four equals treatments: the 1sttreatment (C), bucks served as control. Rabbits of 2nd, 3rd and 4th treatments were exposed to heat shock program. The 2nd treatment (HSE30) exposed at 30 days of age, the 3rd treatment (HSE60) exposed at 60 days of age and the 4th treatment (HSE30+60) exposed at 30+60 days of age. HSP70 and blood metabolites were increased (P≤ 0.05) in the rabbits of HSE groups compared to control group. Triiodothyronine hormone was increased (P≤0.05) in HSE groups as compared to control group. Corticosterone hormone decreased (P≤0.05) in the rabbits of HSE groups as compared to control group. Physical semen quality was improved (P≤0.05) in rabbits of HSE groups as compared to control group. In conclusion, early heat shock exposure programs for buck rabbits might increase release of HSP70 which positive reflect on enhance physiological responses and physical semen characteristics under severe heat stress conditions.
Sustainability of beef cattle farming production system in South Konawe Regency, Southeast Sulawesi A. E. T. Sulfiar; B. Guntoro; B. A. Atmoko; I. G. S. Budisatria
Journal of the Indonesian Tropical Animal Agriculture Vol 47, No 2 (2022): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.47.2.155-165

Abstract

This research aimed to analyze the sustainability of beef cattle farming in smallholder farms with semi-intensive and intensive production systems in the South Konawe Regency. It was conducted in West Ranomeeto and Konda sub-districts through Participatory Rural Appraisal with 55 farmers in the semi-intensive system and 50 farmers in the intensive system. The secondary data were obtained from literature, reports, and publications. The sustainability analysis was determined based on selected indi-cators in each aspect. The indicators of economic aspect were livestock income, feces utilization, sav-ings and insurance. The indicators of environmental aspect were concentration of E. coli, fecal coli-form, total coliform, nitrate, and Fe. The sindicators of social aspect were land ownership, livestock health, mortality, area of pen, and dry matter consumption. The analysis results showed that the total value of the economic, environmental, and social aspect indicators in semi-intensive and intensive pro-duction systems was -0.45 and +0.17; -1.15 and -3.85; -0.10 and +0.27, respectively. Meanwhile, the total indicator value in both production systems was -0.57 and -1.14. It can be concluded that both beef cattle production systems in South Konawe Regency carried out by smallholder farmers have not sus-tainable.
Cholesterol, fatty acid, and micronutrient profiles of IPB D1 chicken meat fed diets supplemented with lemuru fish oil and microminerals L. Latifudin; Z. Wulandari; C. Budiman; C. Sumantri; S. Sumiati; I. I. Arief; N. Nurwulandari
Journal of the Indonesian Tropical Animal Agriculture Vol 51, No 2 (2026): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.51.2.135-148

Abstract

Chicken meat is an important source of animal protein widely consumed worldwide. The IPB D1 chicken is a crossbreed between local chickens and broilers, characterized by rapid growth, high adaptability, and desirable meat characteristics. This study aimed to analyze the effects of different feed formulations on the micronutrient content and lipid profile of IPB D1 chicken meat. A completely randomized design was used with four treatments and three replications. The dietary treatments consisted of control feed (P0), control feed supplemented with Zn and Cr (P1), feed containing lemuru fish oil (P2), and a combination of lemuru fish oil with Zn and Cr (P3). The results indicated that feed formulation significantly affected mineral content, vitamin E concentration, cholesterol level, and fatty acid composition. Treatment P3 produced the most favorable micronutrient and lipid profile, notably increasing mineral content and omega-3 polyunsaturated fatty acids while improving the omega-6 to omega-3 ratio. The P3 feed formulation was the most effective in enhancing micronutrient content and improving lipid profile characteristics of IPB D1 chicken meat.
Effects of rumen-protected fat on changes of metabolites and reproductive genes in testes of Malin rams A. Ismail; A. I. Muhammad; L. T. Kee; L. T. Chwen; A. A. Samsudin
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 2 (2023): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/vol%viss%ipp%p

Abstract

The effects of RPF on metabolites and reproductive genes in testes of Malin ram was investigated. Twenty Malin rams (36.6kg ± 5.57 kg of bodyweight), were subjected to four dietary treatments; A: basal diet without rumen-protected fat (RPF), B; basal diet with 2% prilled fat, C; basal diet with 2% calcium salt and D; basal diet with 2% canola oil. At the end of the experiment four out of five animals from each group were slaughtered. The testes were excised for metabolites and gene expression stud-ies. The genes tested were associated to testes development and spermatogenesis (ODF1, SERPINA10, CatSper4, AdipoR2 and DAZL). Feeding RPF with calcium salt (Treatment C) has resulted in the up-regulation more than two folds in all reproductive genes. There were metabolites changes occurred between the groups and identified 44 important putative metabolites present in the testes. In conclu-sion, feeding of RPF to the animals as a source of energy has up-regulated the genes and identified the metabolites involve in the male reproductive tissues and activities.
Gomperzt non-linear model for predicting growth performance of commercial broiler chickens A. Setiaji; D. A. Lestari; B. Ma&#039;rifah; L. Krismiyanto; I. Agusetyaningsih; S. Sugiharto
Journal of the Indonesian Tropical Animal Agriculture Vol 48, No 2 (2023): June
Publisher : Diponegoro University

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.14710/jitaa.48.2.143-149

Abstract

An experiment was conducted to estimate growth parameters for commercial broiler chickens in Indonesia. The data was collected from July 2021 to June 2022. A total of 1,570 samples consisting of four strains of broiler chickens were collected from 74 houses. The samples were daily weighed from 0 to 7 days of age, and they were weekly weighed from 1 to 5 weeks of age. A nonlinear Gompertz growth model was fitted to the observed body weights (BW). The results for five growth parameters were as follows: the asymptotic value (A) of the mature live weight ranged from 3.733 to 5.044 kg; the turning point of growth (B) ranged from 4.499 to 4.561; the value growth rate constant (K) ranged from 0.049 to 0.059 kg/week; Inflection points in ranged from 25.292 – 30.970 days, and 1.373 – 1.855 kg for inflection age (IA) and inflection weight (IW), respectively. The model was an excellent fit for the growth data in the commercial broiler with a low Akaike information criterion (AIC), and high coeffi-cient determination (R2).

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