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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 8 Documents

Search results for , issue "Vol 5, No 1 (2018)" : 8 Documents clear

The Relationship Among Four Pillars of Diabetes Mellitus Management with Blood Glucose Levels and Nutritional Status in Middle-Aged Diabetic Adults Kanthi Permaningtyas Tritisari; Ayuningtyas Dian Ariestiningsih; Dian Handayani; Inggita Kusumastuty
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Controlling the blood sugar level of DM patients is an approach to suppress morbidity and mortality. This study aims to know the relationship of four pillars of DM management with blood glucose levels and nutritional status in patients with type 2 DM in East Java. This study is a cross-sectional study with a sample of middle-aged type 2 DM outpatients in 4 cities in East Java, Indonesia. Four pillars of management include education profile, food planning, physical exercise, and drug adherence. The statistical analysis used was Partial Least Square. The results of the study show that four pillars were not associated with fasting blood glucose (FBG) level and 2-hour PPG in the total number of respondents (p>0.005). Interestingly, dietary intake links to 2-Hour PPG in 40-50 years age group (p=0.000) and was associated with body mass index in the total number of respondents (p=0.000). Furthermore, education profile and medication also related to FBG in 40-50 years age group (p=0.000, p=0,003). In conclusion, the success of the four pillars in controlling DM is affected by age. In 40-50 years age group, there is an influence of education profile and drug adherence on the level of FBG.

Knowledge and Socioeconomic Status as The Factors of Pre-hospital Delay in Patients with Acute Coronary Syndrome Yustina Ni Putu Yusniawati; Yuyun Yueniwati; Dewi Kartikawatiningsih
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Acute Coronary Syndrome (ACS) is a cardiac emergency condition that requires fast and precise action while a pre-hospital delay in a patient to come to the ED is still common. This pre-hospital delay is related to the socioeconomic and knowledge factor. The purpose of this study is to determine the relationship of socioeconomic and knowledge with the pre-hospital delay of ACS patients to come to Integrated Heart Service in Emergency Department of Sanglah General Hospital, Denpasar. This research is a quantitative research with cross-sectional design and purposive sampling as data collection technique with 292 patients. The results showed that 55.8% of patients have low socioeconomic status and there are 71.6% of patients who do not know ACS. The pre-hospital delay, in this case, is associated with low socioeconomic status and knowledge of ACS so that health workers need to provide counseling to patients and families about ACS signs and symptoms, healthy lifestyles, and the importance of coming to theemergency department when signs of ACS are felt.

Catechins of GMB-4 Clone Inhibits Adipogenesis Through PPAR-γ and Adiponectin in Primary Culture of Visceral Preadipocyte of Rattus Norvegicus Wistar Aswaty Nur; Retty Ratnawati; Diana Lyrawati
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Catechins of green tea (Camelia sinensis) GMB4 clone may serve as a potential therapeutic antiobesity agent, probably through its effects on preadipocytes. Thus, to evaluate such antiobesity effects, we performed series of in vitro experiments using primary cultures of visceral preadipocytes from Rattus norvegicus strain Wistar. Quick Cell Proliferation assay, Oil Red-O staining, ELISA and immunocytochemistry were used to determine the effects of 25 µM, 50 µM, 75 µM, and 100 µM catechins on primary culture of preadipocytes, particularly on cell viability and differentiation as well as on expression of relevant obesity genes i. e. PPARγ and adiponectin levels. The results showed that there were no significant differences on preadipocytes viability among control and catechins treatments except in cells treated with 50 µM catechins (means±SD=128±2.47) which resulted 28% higher viability than control (p= 0.037). Catechins inhibited preadipocytes differentiation into adipocytes, at 100 µM up to 78% lower than control. The level of PPARy apparently was reduced by catechins, but statistically significant only at 75 μM (p= 0.029). In contrast, the adiponectin level on preadipocytes increased by catechins at 75 µM and 100 µM (0.786±0.126 and 0.673 ±0.319 ng/ml; control: 0.077±0.017, p ≤0.01). In conclusions, our data revealed that desired antiobesity effects of catechins of green tea GMB4 clone on visceral preadipocytes were concentration-dependent; at dosage 50 µM catechins enhanced cell viability; at more than 75 µM inhibited differentiation of preadipocytes and was associated with lower PPARy and higher adiponectin levels.

The Linnaeus’s Reed Snake, Calamaria linnaei Boie (Squamata: Colubridae: Calamariinae) from Ijen Plateau, East Java, Indonesia Irvan Sidik; Sutiman Bambang Sumitro; Nia Kurniawan
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The decisive morphological character of Calamaria linnaei from the Ijen plateau was analyzed in an attempt to evaluate these snakes independently achieved as different populations or whether more likely only cryptic species affected by natural conditions. The evidence was concluded that a cryptic species is very interesting to be regarded as something entirely different. In general, however, the population appears to have more closely related to other congeners populations of the same species. It is suggested that at least temporarily, the C. linnaei population of the Ijen plateau be specifically considered to include in species with high individual variations based on limited geographic boundaries until molecular analysis proves it.

Nigella sativa Extract Increases Antibacterial Activity by Up-Regulating T-reg and Th2 Levels in Salmonella enterica subsp. enterica serovars Typhimurium-Exposed Balb/c Mice Zahra Ahmed; Sumarno Reto Prawiro; Agustina Tri Endharti
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Nigella sativa extract (NSE) is known to be an effective antibacterial and immuno-modulating agent. This study aimed to demonstrate that treatment of Salmonella eterica subsp. eterica serovars typhimurium-infected mice with NSE would cause an increase in T-regulatory (T-reg) and Th2 cells and a decrease in the number of spleen bacterial colonies. Mice were randomly divided into five groups [control (T0), mice infected with S. typhimurium only (T1), and mice infected with S. typhimurium and then treated with NSE 0.52, 5.2, or 52 mg/kg body weight NSE (N1, N2, and N3, respectively)]. Levels of T-reg and Th2 cells were determined via flow cytometry and the number of spleen bacterial colonies was determined by observation. The results showed that treatment with NSE 0.52, 5.2, and 52 mg/kg significantly increased the number of T-reg and Th2 cells (p<0.05) relative to T0. Administration of NSE 5.2 mg/kg increased the number of T-reg cells, while administration of NSE 52 mg/kg caused the greatest increase in the number of Th2 cells in the spleens of S. typhimurium-infected mice. Moreover, no S. typhimurium colonies were found in the spleens of any NSE-treated mice. Our results suggest that NSE has therapeutic potential to ameliorate S. typhimurium infection.

Ketamine Improves Sepsis-induced Immunosuppression by Attenuating Intracellular Calcium Elevation in Rat Peripheral Mononuclear Cells Puspita Abidatul Qodariyah; Afiyf Kaysa Waafi; Christian Julio Suhardi; Karina Survival Rofiq; Aswoco Andyk Asmoro; Edi Widjajanto
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Immune response in sepsis divided into two phases, hyper-inflammation (cytokine storm) and hypo-inflammation (immunosuppression). The mechanism of immunosuppression appears to involve apoptosis of immune cells, particularly mononuclear cells and it predisposes to secondary infection that is believed as the predominant driving force for mortality. We aimed to determine the effect of ketamine on mononuclear cells count related to intracellular calcium contained within these cells during the immunosuppression phase of sepsis.This in vivo study was performed in Rattus Novergicus with Fecal Induced Perionitis (FIP) procedure to induce polymicrobial sepsis. Rats were treated with each respective dose of ketamine (2.5, 5, and 10 mg/kg) 1 hour after sepsis induction. Murine Sepsis Score was measured at 1 and 24 hours post-FIP. After 24 hours, animals were sacrificed, and the percentage of intracellular calcium inside CD4, and CD8 T cells, B cells and monocytes, along with these cells counts were determined with flowcytometry. There are significant elevation of intracellular calcium in CD4 T-cells, B-cells and monocytes after FIP-induction and ketamine treatment suppressed this FIP-induced elevation. Measurement of mononuclear cells count showed a relevant result, in which FIP induced mononuclear cells massive loss and ketamine could inhibit the loss. MSS data showed ketamine 5 mg/kg could improve 24 hours MSS with 100% survivability.These findings suggest that ketamine have an inhibitory effect in mononuclear-cells apoptosis mechanism through attenuating intracellular calcium elevation in polymicrobial sepsis. These inhibitory effects of ketamine might correlate with a better survival and clinical outcome.

The Combination of Acupressure and Affirmation Relaxation as an Alternative Method to Increase Breast Milk Production and Breastfeeding Self-efficacy Dita Rahmaika Arumsari; I Wayan Agung Indrawan; Endang Sri Wahyuni
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Breastfeeding has been normative prevailing standard for feeding and fulfilling nutrition for baby due to its important benefit for mothers and her infant. The most common problem in breastfeeding is lack supply of breast milk after giving birth. The aim of this research was to prove that combination of acupressure and affirmation relaxation method was able to increase breast milk and improve breastfeeding self-efficacy of primiparous mothers in the postpartum period. The study design was true experimental with pre-test and post-test design. There were 24 primiparous mothers who were divided into two groups. Weighing test was used to measure breast milk production and breastfeeding self-efficacy was measured using Indonesian version of Breastfeeding Self-Efficacy Scale. Data were analyzed using Unpaired t-test and Mann Whitney test (α = 0.05). Based on the finding, we could conclude that combination of acupressure and affirmation relaxations could improve breast milk production (p = 0.033) and breastfeeding self-efficacy (p = 0.045) for primiparous mother in the postpartum period encountered in several Independent Midwifery Practice in Malang. This method might be used as a non-pharmacological treatment for postpartum mothers. Health practitioners should disseminate this technique so that postpartum mothers can do that by themselves.

Subchronic Toxicity of the Physalis minima Leaves Setyawati Soeharto; Dian Nugrahenny; Nur Permatasari; Elly Mayangsari
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The study was to evaluate the subchronic toxicity of aqueous extract of Physalis minima leaves in female rats. Eighteen female Wistar rats were orally administered with aqueous extract of P. minima leaves at doses of 90, 270 and 450 mg/kg BW, respectively for 90 days. Six female rats without any treatment were provided as a control group. At the end of the experiment, blood samples were collected for hematological and biochemical (aspartate transaminase/AST, alanine transaminase/ALT, creatinine, urea, glucose, cholesterol, and triglyceride) analyses. The results showed that hematological and biochemical biomarkers were not significantly different in treated groups compared to the control group. It is concluded that the aqueous extract of P. minima leaves do not induce hematologic toxicity, hepatotoxicity and renal toxicity in the subchronic toxicity study.

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Kuswanto

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Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

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Journal Mail Official rjls@ub.ac.id

Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Kuswanto

Contact Email
rjls@ub.ac.id

Phone
+62341 575825

Journal Mail Official
rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA