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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 202 Documents

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The Refugia Attract Arthropods in a Paddy Field in Malang, East Java, Indonesia Amin Setyo Leksono; Jati Batoro; Anisa Zairina
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

A study on the effect of refugia areas to attract on Arthropods has been done in a semi-organic paddy field in Malang, from March to June 2017. The arthropod was measured by abundance, diversity and composition variables. Arthropod observations were performed on 6 plots of 1 m2 each, consisting of 3 plots on the side near the Refugia area (treatment) and 3 plots on the opposite side (control). The refugia areas consisted of plants as follows chili (Capsicum frutescens), the wild cosmos (Cosmos caudatus), the long beans (Vigna unguiculata), and Marigold (Tagetes erecta). The visual encounter surveys method was conducted for 15 minutes on each plot. There were 2249 individuals of Arthropod observed visually in the study sites. This study showed that of the abundance (mean ± SE) of Arthropod visitors was lower in plots adjacent to refugia areas (treatment) (33.7± 2.63), than that far from Refugia area (control) (38.33 ± 2.54); while the taxa richness and diversity was higher found in treatment plots. The species richness in the treatment plots (12.2 ± 0.70) was higher than that in control (9.70 ± 0.51). The diversity of Arthropod visitors in the treatment plots (2.10 ± 0.07) was also higher than that in control (1.71 ± 0.07). Proportion of predator in the treatment plots was almost twice higher (33.14) that in the control plots (17.65); while that of herbivore was vice versa. This meant the refugia areas have attracted more predators. The composition of Arthropod visitors was remarkable affected by treatment. The abundance of common predator families such as Coccinellidae, Coenagrionidae and Oxyopidae were higher in treatment plots.

Subchronic Toxicity of the Physalis minima Leaves Setyawati Soeharto; Dian Nugrahenny; Nur Permatasari; Elly Mayangsari
Research Journal of Life Science Vol 5, No 1 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The study was to evaluate the subchronic toxicity of aqueous extract of Physalis minima leaves in female rats. Eighteen female Wistar rats were orally administered with aqueous extract of P. minima leaves at doses of 90, 270 and 450 mg/kg BW, respectively for 90 days. Six female rats without any treatment were provided as a control group. At the end of the experiment, blood samples were collected for hematological and biochemical (aspartate transaminase/AST, alanine transaminase/ALT, creatinine, urea, glucose, cholesterol, and triglyceride) analyses. The results showed that hematological and biochemical biomarkers were not significantly different in treated groups compared to the control group. It is concluded that the aqueous extract of P. minima leaves do not induce hematologic toxicity, hepatotoxicity and renal toxicity in the subchronic toxicity study.

Allele Frequencies of STR CODIS 13 of Madura Ethnic from Bangkalan and Probolinggo Wening Prastowo; Diana Lyrawati; Sri Andarini; Karyono Mintaroem
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

One of forensic identification that can be done is through DNA testing. FBI recommends to use 13 short tandem repeat loci known as CODIS 13 for forensic DNA testing. We tested such system to characterize and determine whether individuals with Madura ethnic background (Madurese) resides in different region differ in CODIS 13 pattern. Employing standard PCR-RFLP for STR CODIS 13 our results showed that indeed the CODIS 13 pattern between the two regions were distinct. Most frequent alleles appeared in Madura ethnic from Bangkalan were at locus D3S1358: allele 16; VWA: 16; FGA: 24 and 26; TH01: 8; TPOX: 9; CSF1PO: 10; D5S818: 11 and 13; D13S317: 14; D7S820: 9; D8S1179: 15; D21S11: 27, 28 and 30; D18S51: 16; and D16S539: 15. Most frequent alleles appeared in each locus of Madura ethnic from Probolinggo were locus D3S1358: allele 17; VWA: 17; FGA: 23 and 26; TH01: 7; TPOX: 10; CSF1PO: 10, 11 and 13; D5S818: 11 and 12; D13S317: 11; D7S820: 11; D8S1179: 11 and 12; D21S11: 30; D18S51: 14; and D16S539: 8. The heterozygosity levels of Madura ethnic from Bangkalan’s CODIS 13 varied from 0.4180 (D5S818) to 0.9102 (FGA). The power of exclusion was between 0.1569 (D5S818) to 0.7667 (FGA). The power of discrimination was between 0.2816 (D5S818) to 0.9811 (FGA). The paternity index was between 0.8591 (D5S818) to 5.5653 (FGA). The heterozygosity index of Madura ethnic from Probolinggo varied between 0.63021 (VWA) to 0.94445 (FGA), power of exclusion was between 0.33418 (VWA) to 0.84779 (FGA), and power of discrimination ranged from 0.60501 (TPOX) to 0.99305 (FGA).

Hematological Analysis of Nile Tilapia (Oreochromis niloticus) and Striped Catfish (Pangasius hypophthalmus) using Hematology Analyzer Tool Software at Fish Breeding Center Jojogan, Tuban, East Java Maftuch Maftuch
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Nile tilapia and Striped Catfish can be classified as freshwater fish species that have great cultivation potential. Inadequate cultivation management leads to the occurrence of disease attacks on fish. Hematological examination was applied to determine the level of fish health. This research was aimed to know the difference between erythrocyte cell and leucocyte cell count by using Hematology Analyzer Tool Software (computer based) and hand tally counter (human based), and to know the description of hematocrit component, hemoglobin and leucocytes differential on tilapia fish and Striped Catfish. The methods employed in this research were experimental method and descriptive method. The results showed that the calculation of erythrocyte cells and leucocyte cells by using Hematology Analyzer Tool Software was similar to the calculation by using hand tally counter. It was because the result of t value was smaller than t of the 5% table. Based on the description of the hematocrit component, hemoglobin and leucocyte differentials, it could be said that nile tilapia and Striped Catfish were within the range of abnormalities.

Analysis of The Secondary Metabolite of Kersen Leaf Extracts (Muntingia calabura L.) and Its Potential as Anti-Bacteria to Inhibit Aeromonas hydrophila Gede Angga Krishna Fariestha; Sri Andayani; Uun Yanuhar
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Aeromonas hydrophila is a gram-negative bacterium that massively attack aquatic organisms in fresh water, briny water and sea waters. Infection due to this bacterium caused septicemia in the host's body to the point of damaging the body's organs. Hence, alternative material is required in order to cope with this bacterium, which was by using natural material such as kersen leaf (Muntingia calabura L). The purpose of the research is to determine the effect of secondary metabolite contained in kersen leaf as anti-bacterial against Aeromonas hydrophila. This research used a method of MIC (Minimum Inhibitory Concentration) as an anti-bacterial test. In order to examine the secondary metabolite content, phytochemical screening and FTIR (Fourier Transform Infrared Spectroscopy) methods were used. The results obtained through the MIC test of 125 ppm is the minimum concentration capable of inhibiting the growth of Aeromonas hydrophila. Kersen leaf extracts is bacteriostatic anti-bacteria which only inhibit bacterial growth up to 24 hours. Phytochemical screening reported that kersen leaf extracts were positive of flavonoids, tannins, saponins, alkaloids and triterpenoids. Through FTIR test, it is known that phenol compounds were contained in kersen leaf extracts. This phenol is one of the metabolites with the function of anti-bacterial on this study.

Influence of Light Intensity on Lipid Productivity and Fatty Acids Profile of Choricystis sp. LBB13-AL045 for Biodiesel Production Swastika Praharyawan; Delicia Yunita Rahman; Dwi Susilaningsih
Research Journal of Life Science Vol 5, No 2 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The influence of various light intensity on the lipid productivity, fatty acids profile, and biodiesel properties of Choricystis sp. AL045 were examined. Choricystis sp. LBB13-AL045 was a high lipid content microalgal strain (up to 42.49 ± 0.41% per dry weight basis) with great performance of its growth (specific growth rate and biomass productivity were up to 0.802 ± 0.013 day-1 and 108.57 ± 8.07 mg L-1 day-1, respectively). Such results indicated its high lipid productivity and its potency to be used for biodiesel production. The treatment of various light intensities on the microalgal culture resulted obvious differences in lipid productivity and fatty acids composition. Maximum lipid productivity (46.13 ± 3.43 mg L-1 day-1) was at 405 µmol m-2 s-1 of light intensity. The fatty acids profiles of four light intensities treatments were quite similar; the major components of fatty acid obtained from Choricystis sp. LBB13-AL045 oil were oleic acid (C18:1) and palmitic acid (C16:0), which provided a strong indication of high-quality biodiesel. Biodiesel properties were determined by empirical equations and found to be within the limits of biodiesel standard SNI 7182:2015, ASTMD6751-08 and EN14214.

The Effect of Ulva lactuta Polysaccharide Extract on Total Haemocyte Count and Phagocytic Activity of L. vannamei Suleman Suleman; Sri Andayani; Ating Yuniarti
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

This research aimed to evaluate the effect of polysaccharide extract of Ulva lactuta on the total haemocytes count (THC) and the phagocytic activities (PA) on Litopenaeus vannamei. The concentration of the extract used in this research was 5 ppm and it was applied through the immersion. The haemolymph was collected after the administration in the different periods at hour of 4 and 24. The results showed that the administration of polysaccharide extracts increased THC and PA in L. vannamei significantly (p<0.05). THC and PA of white-leg shrimp with polysaccharide extract was higher than that without the administration of the extract. The enhancement of THC was found after the administration at hour 4 from 5.00 cells. ml-1 to 5.26 x 104 cells. ml-1. However, the THC level decreased after 24 hours at the value of 5.19 x 104 cells. The increasing pattern was also true for PA of L. vannamei, from 40.44% to 53.67% (hour 4). At the hour of 24, the PA was found decreased to the level and then decreased 45.67 ±1.53. On the contrary, there were no enhancement of THC and PA in the treatment without polysaccharide extract of Ulva lactuta.

Antioxidant Effect of Centella asiatica Ethanolic Extract to Superoxide Dismutase (SOD) Level on Cyprinus carpio Liver Diklawati Jatayu; Happy Nursyam; Asus Maizar Suryanto Hertika
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Centella asiatica is a herbal medicine has been used for many studies in some animals as antioxidant properties. This study was aimed to enhance the level of SOD in Cyprinus carpio after oral administration of C. asiatica extract. In the present study, phytochemical screening of bioactive constituents, antioxidant assay, and their effect on SOD in C. carpio liver were investigated. C. asiatica extract was obtained by maseration for 24 h using ethanol as a solvent. C. asiatica extract with 50, 100, 150 and 300 mg kg-1 body weight were administered every 3 days for two weeks. On the last day of experiment, fishes were killed and liver were removed from the body. The results showed that C. asiatica extract had some bioactive compounds such as flavonoid, alkaloid, terpenoid, tanin, saponin and DPPH scavenging activity with an IC50 value of 125 μg mL-1. C. asiatica extract also enhanced the level of SOD in fish liver. The SOD level was significantly difference compared to the control group with P<005. These results indicate that the ethanolic extract of C. asiatica had a potential antioxidant properties by scavenging free radical and enhancing the SOD level as antioxidant deffense inside the body.

Association Between Time Spent for Internet Gaming, Grade Point Average and Internet Gaming Disorder Risk Among Medical Students Nia Kurnianingsih; Retty Ratnawati; Dearisa Surya Yudhantara; Rizqi Bagus Setyo Prawiro; Maryam Permatasari; Heidyana Rachma; Adilla Surya Ariadi
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The negative and positive consequences of internet games were less explored among medical students’ population. The aim of this current study was to investigated the association between time spent for playing internet games and Grade Point Average (GPA) as well as Internet Gaming Disorder (IGD) risk among medical students. A total of 438 students in academic year 2016-2017 completed the self-report questionnaire, that covered socio-demographic information, GPA, time spent for internet gaming/day, and IGD screening. The statistical analysis used c2 test. The average age of the respondents was 20.2 ±1.57 year. The respondents comprised of 37.13% male and 62.87% female students. The percentage of male and female players was 97.5% and 93.8% respectively. Male students were more at risk of IGD (29.56%) compared to their female counterparts (10.04%). Although time spent for internet gaming/day was associated with IGD risk (p<0.001), it was not associated with GPA (p=0.501). There was no significant GPA difference between students who played internet games <3 hours/day and >3 hours/day and those who never play at all. Further study is required to explore the factors related to GPA among medical students.

Activity of Compounds on Seaweed Eucheuma cottonii Extract as Antioxidant Candidate to Prevent Effects of Free Radical in Water Pollution Dewi Wulandari; Yuni Kilawati; Mohamad Fadjar
Research Journal of Life Science Vol 5, No 3 (2018)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Water pollution can make many problems such as, the incidence of disease and poisoning. Pollution in water can produce free radicals and it is can trigger of disease for aquatic organisms. There is some efforts that can be done to provide this problem, such as chemical compound that can reduce the reaction of free radicals. Antioxidants are one of the chemical compounds that can reduce the activity of free radicals. Eucheuma cottonii is the one of a seaweed that has many in antioxidant compounds, such as phenol compounds, but it is also rich in iodine fiber and other important minerals. The method used in this research is descriptive explorative and experimental method. This research was conducted with several stages of seaweed extraction. Identification of Eucheuma cottonii extract is using FTIR test. The last stage is an antioxidant activity test that includes DPPH test (1,1-diphenyl-2-picrilhidrazil) and Inhibition Concentration 50 (IC 50). The results obtained in this study were based on FTIR test of antioxidant compound in Eucheuma cottonii seaweed extract. The one of compounds that have antioxidant activity include galaktosa-4-sulfat. Based on the results of antioxidant activity test using DPPH obtained that seaweed extract Eucheuma cottonii active as an antioxidant to ward off free radicals in the waters. The concentration of Eucheuma cottonii seaweed extract for preventive 50% concentration of DPPH is 39,926 ppm.

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Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Kuswanto

Contact Email
rjls@ub.ac.id

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+62341 575825

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rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA