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Research Journal of Life Science

rjls
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Published by Universitas Brawijaya

ISSN : - EISSN : 23559926 DOI : https://doi.org/10.21776/ub.rjls

Core Subject : Health, Science, Agriculture,

Agriculture, Biological Sciences & Forestry Biochemistry, Genetics & Molecular Biology Medicine & Pharmacology

Research Journal of Life Science (RJLS) is an open access publishes papers three times a year that emphasize the agriculture, fisheries, animal science, medicine, basic science and food technology. All articles are rigorously reviewed by reviewers. Articles should be created in a language that is easily understood by non-specialist readers in the topic of the article itself, but interested in research. The main objective of RJLS is to provide a platform for the international scholars, academicians and researchers to share the contemporary thoughts and innovations in the field of life science. RJLS aims to promote studies in life science and so become the leading international journals in life science in the world.This journal intends to provide facilities to advance the development and application of interdisciplinary and transdisciplinary research methodologies in life sciences.

Arjuna Subject : Ilmu Pertanian dan Biologi (Semua) - Ilmu Pertanian dan Biologi (Lain-Lain)

Articles 202 Documents

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Antibacterial Activity of Dayak Onion Bulbs (Eleutherine palmifolia (L) Merr) Ethanol Fraction against Pseudomonas fluorescens and Its Secondary Metabolite Analysis Immaria Fransira; Annisa Fitri Anggreini; Uun Yanuhar; Maftuch Maftuch
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Pseudomonas fluorescens is a gram-negative pathogenic bacteria which is one of the problems in fish farming that can causes death in fish. The alternative to overcome this bacterial attack is by using of natural material such as dayak onion bulbs (Eleutherine palmifolia (L) Merr), which are known to have secondary metabolites. The purpose of this research was to determine the antibacterial activity against P. flourescens that contained in the dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction and the content of antibacterial compounds from the best dayak onion bulbs (E. palmifolia (L) Merr) ethanol fraction. The method used in this research was antibacterial test with MIC (Minimum Inhibitory Concentration) followed by disc test and FTIR (Fourier Transform Infrared Spectroscopy) to analysis secondary metabolite. Fractionation was conducted using Chloroform: Methanol (9:1 v/v). MIC test result showed that 100 ppm was the minimum concentration that can inhibit the growth of P. fluorescens. Disc test reported that fraction 4 was the best fraction, with the dominant compounds that contained in the bulbs was phenolic compound based on FTIR test. So, the fraction of dayak onion bulbs (E. palmifolia (L) Merr) can be used as potential leads to discover new drugs.

Active Compounds on Squid (Loligo sp.) Ink Extract Powder as Immunostimulant Candidate to Against Shrimp Disease Rangga Idris Affandi; Mohamad Fadjar; Arning Wilujeng Ekawati
Research Journal of Life Science Vol 6, No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The business of developing shrimp farming cannot be separated from the existence of disease. In shrimp farming health management, disease prevention strategies can using various methods, one of which is immunostimulant. One alternative immunostimulant source that can be used to improve the body defense system in shrimp is squid (Loligo sp.) ink extract powder who has antibacterial ability. This study aims to analyze the presence of active compound in squid ink extract powder can be used as an immunostimulant candidate to against shrimp disease. The method used in this research is descriptive explorative and experimental method. This research was conducted with several stages of squid ink extraction until it becomes powder. Identification of squid ink extract powder is using FTIR and LC-MS test. The results showed that the squid ink extract powder contained alkaloid and carboxylic acid from the FTIR test results. Based on LC-MS test results, it was found that squid ink extract powder contained betaine, cinnamic acid, and choline compounds with large amounts of content. Betaine, cinnamic acid, and choline has several biological activity as antibacterial, antioxidant, antiviral, antifungal, etc. so that it can be used as an immunostimulant against shrimp disease.

Calprotectin and Intestinal Fatty Acid Binding Protein (I-FABP) Level in Preterm Neonates with Necrotizing Enterocolitis Ditya Arisanti; Satrio Wibowo; Soemarno Soemarno
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Necrotizing enterocolitis (NEC) is inflammatory state of intestinal tissue which mostly occurred in preterm neonates and associated with ischemia and inflammation. This study was aimed to investigate calprotectin level (inflammatory marker) and intestinal fatty acid binding protein (I-FABP) (ischemia marker) in preterm neonates with NEC. This research was designed as cross sectional which involve 32 preterm neonates divided into 2 groups as follows: NEC group (n=16 subjects) and control group (n=16 subjects). Diagnosis of NEC was established by clinical and radiological signs (abdominal distension and pneumatosis). Fecal calprotectin and urinary I-FABP were measured using ELISA method. Results showed that fecal calprotectin and urinary I-FABP was significantly higher in NEC group as compared to control group (Mann-Whitney test, p<0.05). Both calprotectin and I-FABP was positively correlated with NEC state (Spearman correlation test, p=0.000, r=0.866). Moreover, calprotectin and I-FABP level was positively correlated with grade (Bell’s classification) and type (Gordon’s classification) of NEC (Spearman correlation test, p<0.05). We concluded that calprotectin and I-FABP level was higher in NEC group. Moreover, I-FABP but not calprotectin, were correlated with grade and type of NEC.

Phytochemical analysis, FTIR fingerprint and bioactivity of crude extract and fractions of Mesua ferrea L. Dwi Hartanti; Zainur Rahman Hakim; Anna Nurlativah; Arinda Nur Cahyani; Fadhilah Zahra Nadia; Asmiyenti Djaliasrin Djalil
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.7

Mesua ferrea L. (Ceylon ironwood, Calophyllaceae) is a popular medicinal plant with a long history in South and Southeast Asia folk medicines. In this study, we report the phytochemical analysis, FTIR fingerprint, as well as antimicrobial and antioxidant activities of the crude extract of leaves of M. ferrea and its fractions. The dried leaves of M. ferrea were extracted with ethanol by the re-maceration method. The crude extract was further partitioned in ethanol-water and ethyl acetate to obtain ethanol and ethyl acetate fractions, respectively. The identification of compounds in the extract and fractions was conducted according to the standard phytochemical screening method. Fourier-transform infrared spectroscopy (FTIR) was utilized to record their metabolites fingerprint. The antimicrobial activity was evaluated with the disk diffusion method against Escherichia coli, Bacillus subtilis, Candida albicans, and Saccharomyces cerevisiae. The antioxidant activity assay was conducted with 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging method. The crude extract of M. ferrea leaves and it fractions contained saponins, phenolic compounds, flavonoids, and terpenoids. The FTIR data supported the presence of terpenoids and glycosides in those samples as well. The extract and fractions demonstrated considerable antimicrobial activity against all tested bacteria and fungi, with MIC values ranged 3.9-31.3 μg/ml. The ethyl acetate fraction of M. ferrea leaves showed a moderate antioxidant activity with an IC50 value of 49.19 μg/ml.

Mental Health of Human trafficking; A Systematic Review Awaliyah Muslimah Suwetty; Asti Melani Astari; Titin Andri Wihastuti
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Human trafficking is being global crime worldwide. The practice of human trafficking in the form of human exploitation implicated in human rights violations. Survivors of human trafficking will experience health problems both physical and mental problems. The purpose of this systematic review was to identify and summarize the mental health problems of human trafficking victims. Systematic search conducted on March 12, 2019 in data of Proquest, Springer Link, and Scopus by keyword *human health of human trafficking* between the years of 2013-2018. The result of this search found 14 journals that match with inclusion criteria. The inclusion criteria were peer-reviewed journal articles, written in English, all forms of exploitation, the samples are aged >15 years old. The results shown the incidence of human trafficking greatly affected the mental health of victim. Victims experienced anxiety, depression, isolation, disorientation, aggression, suicidal ideation, attention deficit, psychotic disorders, behavioral and post-traumatic stress disorder (PTSD). For further research required in efforts to overcome the mental health impact of human trafficking victims. The focus on preventing efforts was through government campaigns, IOM and law enforcement and also the fulfillment of the rights of human trafficking victims

Physical and Organlopetic Properties of Nuggets from Domestic Chicken (Gallus domesticus) Meat with Different Corn Flours as Filler Wahidah Ma'ruf; Djalal Rosyidi; Lilik Eka Radiati; Purwadi Purwadi
Research Journal of Life Science Vol 6, No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The research aims to understand the physical and organoleptic properties of the Indonesian domestic chicken (Gallus domesticus) nugget made with different corn flours as filler. The research was done in two research steps. The first step was to observe the physical properties of the chicken nugget through a completely randomized design, where five different corn flours were used and measured for six times each. The second step was to observe the organoleptic properties of the chicken nugget through two-factorials completely randomized design, where five different panellists (P1, P2, P3, P4, and P5) were the first factor and different corn flours were the second factor. The corn flours used in this research were yellow-corn (J1); fluffy-corn (J2); purple-corn (J3); Provit A1-corn (J4); and Provit A2-corn (J5) flour. The measured variables include cooking loss, flavor, aroma, texture, and color of the chicken nugget. The results showed that J1 chicken nugget had the lowest cooking loss at 1.27%, while the organoleptic measurements showed that J4 chicken nugget had the best organoleptic properties. The research concluded that the usage of Provit A1-corn (J4) flour would give the best overall physical and organoleptic properties of chicken nugget made from domestic chicken meat.

The Effect of Mindfulness Meditation on Mental Illness among Nurse in ICU and ICCU Debby Hatmalyakin; Yulian Wiji Utami; Titin Andri Wihastuti
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Critical conditions as one of the main focused in hospital care. This condition required rapid, comprehensive and quality management that carried out by nurses especially in the treatment of cardiovascular disease. This condition caused pressure on the nurse and causes stress. This stress can be overcome by complementary and alternative therapy in the form of mindfulness meditation. The purpose of this study was to identify the effect of mindfulness meditation therapy in reducing stress on nurses in ICU and ICCU. This study is quasy experiment research design through one group pretest posttest approach with respondents were 14. The results of this study indicated that there was decreased in stress before and after intervention with p-value=0000 and stress before and 1 week after intervention with p-value=0.001. Mindfulness therapy can reduce nurse stress.

Correlation between Density of Vibrio Bacteria with Oscillatoria sp. Abundance on Intensive Litopenaeus vannamei Shrimp Ponds Heri Ariadi; Mohammad Mahmudi; Mohamad Fadjar
Research Journal of Life Science Vol 6, No 2 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

The abundance of Vibrio bacteria and the presence of an excess Oscillatoria sp plankton type are the main problems that often arise in intensive vanamei (Litopenaeus vannamei) shrimp culture. To examine this problem, the purpose of this study was to determine of model approach interaction between the abundance of Vibrio and Oscillatoria sp with the shrimp growth rate in ponds. This research was conducted with the ex-pose facto design on intensive shrimp culture operations in Bayeman Village, Probolinggo. The results data from field research variable are analyzed using a dynamic modeling system. From the modeling analysis results, showed that the Oscillatoria sp abundance pattern tended to increase over with shrimp culture period as following the pattern of tropical status dynamics by the nutrient increase load of 16.37%/week in ponds ecosystem. Meanwhile, the increase of Vibrio colonies density in ponds continued to increase aggregately by 0.99%/week on nine weeks and 12.5% / week on the last eight weeks of shrimp culture periods. So, it can be concluded that the fluctuations density of vibrio and Oscillatoria sp bacteria in ponds is are bioecological responses from increased nutrient loads and other micromaterials in ponds due to the longer period of shrimp culture.

Simulation of Tumor Growth Model and Its Interaction with Natural-Killer Cells and T Cells Cholifatul Maulidiah; Trisilowati Trisilowati; Ummu Habibah
Research Journal of Life Science Vol 6, No 3 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.03.5

This research studies about tumor growth model by involving immune system. Cells in the immune system, for instance natural killer (NK) cells and T cells, have prominent role in recognizing and eliminating tumor cells. In this paper, we construct the tumor growth model consisting of four populations namely tumor cells, NK cells, CD8+T cells, and CD4+T cells which is in the form of a non-linear differential equation. The analysis result shows that there are three tumor free equilibrium points and one coexisting equilibrium point. Some tumor free equilibrium and tumor equilibrium point exist and it is stable under certain conditions. Finally, numerical simulation is carried out to illustrate analysis result. From sensitivity analysis, it is found that the most sensitive parameter that influence the growth rate of tumor cells are the reciprocal carrying capacity of tumor cells and the killing rate of CD8+T cells by tumor cells.

Evaluation of Urine Procalcitonin, Interleukin-6, Heparin Binding Protein, Leukocyturia, and Bacteriuria for Early Detecting Urinary Tract Infection Hani Susianti; I Putu Adi Santosa; Antonius Johanes; Esther Mayrita; Thiba Dina Merdikaningsih
Research Journal of Life Science Vol 6, No 1 (2019)
Publisher : Lembaga Penelitian dan Pengabdian kepada Masyarakat, Universitas Brawijaya

Show Abstract | Download Original | Original Source | Check in Google Scholar | DOI: 10.21776/ub.rjls.2019.006.01.2

Urinary Tract Infection (UTI) is a common infectious disease. Urine culture as a gold standard is less practical and time- consuming. Examination of urine dipstick and microscopic is less accurate. Procalcitonin (PCT), IL-6 and Heparin Binding Protein (HBP) have known increase after infection, which potentially used for early detection of UTI. The aim of this study was to determine the diagnostic performance of urine IL-6, HBP, PCT, leukocyturia and bacteriuria level as biomarkers of UTI. The study was conducted on 51 UTI patients with positive urine culture, 16 UTI patients with negative urine culture, and 16 healthy control. Urine IL-6, HBP, and PCT level were determined using ELISA. Leukocyturia and bacteriuria were measured using automated flowcytometry and fluorescent dye analyzers. The gold standard for UTI diagnosis was urine culture. The sensitivity and specificity of urine PCT, IL-6, HBP, leukocyturia and bacteriuria were 60.78% and 65.62%; 72.55% and 56.25%; 96.07% and 50.00%; 80.39% and 78.12%; 80.39% and 59.09% respectively. The AUC of urine PCT, IL-6, HBP, leukocyturia and bacteriuria were 0.600; 0.605; 0.666; 0.877 and 0.830 respectively. For combinations of two biomarkers, the best sensitivity and specificity was demonstrated by urine PCT and leukocyturia.

Page 12 of 21 | Total Record : 202

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Editorial Address Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location Kota malang, Jawa timur INDONESIA

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Contact Name
Kuswanto

Contact Email
rjls@ub.ac.id

Phone
+62341 575825

Journal Mail Official
rjls@ub.ac.id

Editorial Address
Directorate of Research and Community Service, Gedung Layanan Bersama, 7th Floor, Jl. Veteran Malang, Universitas Brawijaya, 65145, Jawa Timur, Indonesia

Location
Kota malang,

Jawa timur

INDONESIA